2hh7 - Revision history

OCA at 09:32, 14 February 2024

OCA — Wed, 14 Feb 2024 09:32:38 GMT

←Older revision		Revision as of 09:32, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='2hh7' size='340' side='right'caption='[[2hh7]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='2hh7' size='340' side='right'caption='[[2hh7]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus~~ tuberculosis~~" (zopf 1883) klein 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh7 OCA], [https://pdbe.org/2hh7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hh7 RCSB], [https://www.ebi.ac.uk/pdbsum/2hh7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hh7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh7 OCA], [https://pdbe.org/2hh7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hh7 RCSB], [https://www.ebi.ac.uk/pdbsum/2hh7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hh7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CSOR_MYCTU CSOR_MYCTU]] Copper-sensitive repressor that has a key role in copper homeostasis. It is part of the cso operon involved in the cellular response to increasing concentrations of copper inside the bacterium, which can be highly toxic. In the presence of copper, CsoR fully dissociates from the promoter in the cso operon, leading to the transcription of its genes. Binds to a GC-rich pseudopallindromic sequence, 5'-GTAGCCCACCCCCAGTGGGGTGGGA-3', in the cso promoter region.<ref>PMID:17143269</ref>	+	[https://www.uniprot.org/uniprot/CSOR_MYCTU CSOR_MYCTU] Copper-sensitive repressor that has a key role in copper homeostasis. It is part of the cso operon involved in the cellular response to increasing concentrations of copper inside the bacterium, which can be highly toxic. In the presence of copper, CsoR fully dissociates from the promoter in the cso operon, leading to the transcription of its genes. Binds to a GC-rich pseudopallindromic sequence, 5'-GTAGCCCACCCCCAGTGGGGTGGGA-3', in the cso promoter region.<ref>PMID:17143269</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hh7 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hh7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Copper is an essential element that becomes highly cytotoxic when concentrations exceed the capacity of cells to sequester the ion. Here, we identify a new copper-specific repressor (CsoR) of a copper-sensitive operon (cso) in Mycobacterium tuberculosis (Mtb) that is representative of a large, previously uncharacterized family of proteins (DUF156). Electronic and X-ray absorption spectroscopies reveal that CsoR binds a single-monomer mole equivalent of Cu(I) to form a trigonally coordinated (S(2)N) Cu(I) complex. The 2.6-A crystal structure of copper-loaded CsoR shows a homodimeric antiparallel four-helix bundle architecture that represents a novel DNA-binding fold. The Cu(I) is coordinated by Cys36, Cys65' and His61' in a subunit bridging site. Cu(I) binding negatively regulates the binding of CsoR to a DNA fragment encompassing the operator-promoter region of the Mtb cso operon; this results in derepression of the operon in Mtb and the heterologous host Mycobacterium smegmatis. Substitution of Cys36 or His61 with alanine abolishes Cu(I)- and CsoR-dependent regulation in vivo and in vitro. Potential roles of CsoR in Mtb pathogenesis are discussed.
-
-	CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator.,Liu T, Ramesh A, Ma Z, Ward SK, Zhang L, George GN, Talaat AM, Sacchettini JC, Giedroc DP Nat Chem Biol. 2007 Jan;3(1):60-8. Epub 2006 Dec 3. PMID:17143269<ref>PMID:17143269</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2hh7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ramesh, A~~]]	+	[[Category: Mycobacterium tuberculosis]]
-	[[Category: ~~Sacchettini, J C~~]]	+	[[Category: Ramesh A]]
-	[[Category: ~~4-helix bundle]]~~	+	[[Category: Sacchettini JC]]
-	~~[[Category: Unknown function~~]]	+

OCA at 11:08, 5 January 2022

OCA — Wed, 05 Jan 2022 11:08:53 GMT

←Older revision		Revision as of 11:08, 5 January 2022
Line 3:		Line 3:
	<StructureSection load='2hh7' size='340' side='right'caption='[[2hh7]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='2hh7' size='340' side='right'caption='[[2hh7]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh7 OCA], [~~http~~://pdbe.org/2hh7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2hh7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2hh7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2hh7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh7 OCA], [https://pdbe.org/2hh7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hh7 RCSB], [https://www.ebi.ac.uk/pdbsum/2hh7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hh7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CSOR_MYCTU CSOR_MYCTU]] Copper-sensitive repressor that has a key role in copper homeostasis. It is part of the cso operon involved in the cellular response to increasing concentrations of copper inside the bacterium, which can be highly toxic. In the presence of copper, CsoR fully dissociates from the promoter in the cso operon, leading to the transcription of its genes. Binds to a GC-rich pseudopallindromic sequence, 5'-GTAGCCCACCCCCAGTGGGGTGGGA-3', in the cso promoter region.<ref>PMID:17143269</ref>	+	[[https://www.uniprot.org/uniprot/CSOR_MYCTU CSOR_MYCTU]] Copper-sensitive repressor that has a key role in copper homeostasis. It is part of the cso operon involved in the cellular response to increasing concentrations of copper inside the bacterium, which can be highly toxic. In the presence of copper, CsoR fully dissociates from the promoter in the cso operon, leading to the transcription of its genes. Binds to a GC-rich pseudopallindromic sequence, 5'-GTAGCCCACCCCCAGTGGGGTGGGA-3', in the cso promoter region.<ref>PMID:17143269</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	==See Also==		==See Also==
	*[[Copper homeostasis protein\|Copper homeostasis protein]]		*[[Copper homeostasis protein\|Copper homeostasis protein]]
-	*[[Molecular Playground/CsoR and RcnR\|Molecular Playground/CsoR and RcnR]]
-	*[[Molecular Playground/Metal Responsive Regulators-Fall2001\|Molecular Playground/Metal Responsive Regulators-Fall2001]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:09, 1 April 2020

OCA — Wed, 01 Apr 2020 10:09:46 GMT

←Older revision		Revision as of 10:09, 1 April 2020
Line 1:		Line 1:

	==Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.==		==Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.==
-	<StructureSection load='2hh7' size='340' side='right' caption='[[2hh7]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='2hh7' size='340' side='right'caption='[[2hh7]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>
Line 37:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ramesh, A]]		[[Category: Ramesh, A]]
	[[Category: Sacchettini, J C]]		[[Category: Sacchettini, J C]]
	[[Category: 4-helix bundle]]		[[Category: 4-helix bundle]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 06:55, 27 June 2018

OCA — Wed, 27 Jun 2018 06:55:13 GMT

←Older revision		Revision as of 06:55, 27 June 2018
Line 1:		Line 1:
		+
	==Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.==		==Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.==
	<StructureSection load='2hh7' size='340' side='right' caption='[[2hh7]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='2hh7' size='340' side='right' caption='[[2hh7]], [[Resolution\|resolution]] 2.55Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh7 OCA], [http://pdbe.org/2hh7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hh7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hh7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh7 OCA], [http://pdbe.org/2hh7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hh7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hh7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hh7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/2hh7_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/2hh7_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hh7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 37:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Ramesh, A]]		[[Category: Ramesh, A]]
-	[[Category: Sacchettini,J C]]	+	[[Category: Sacchettini, J C]]
	[[Category: 4-helix bundle]]		[[Category: 4-helix bundle]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 08:08, 11 September 2015

OCA — Fri, 11 Sep 2015 08:08:00 GMT

←Older revision		Revision as of 08:08, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2hh7' size='340' side='right' caption='[[2hh7]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='2hh7' size='340' side='right' caption='[[2hh7]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis Mycobacterium~~ tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hh7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hh7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh7 OCA], [http://pdbe.org/2hh7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hh7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hh7 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CSOR_MYCTU CSOR_MYCTU]] Copper-sensitive repressor that has a key role in copper homeostasis. It is part of the cso operon involved in the cellular response to increasing concentrations of copper inside the bacterium, which can be highly toxic. In the presence of copper, CsoR fully dissociates from the promoter in the cso operon, leading to the transcription of its genes. Binds to a GC-rich pseudopallindromic sequence, 5'-GTAGCCCACCCCCAGTGGGGTGGGA-3', in the cso promoter region.<ref>PMID:17143269</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2hh7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Mycobacterium tuberculosis]]
	[[Category: Ramesh, A]]		[[Category: Ramesh, A]]
	[[Category: Sacchettini,J C]]		[[Category: Sacchettini,J C]]
	[[Category: 4-helix bundle]]		[[Category: 4-helix bundle]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 13:39, 25 December 2014

OCA — Thu, 25 Dec 2014 13:39:44 GMT

←Older revision		Revision as of 13:39, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hh7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hh7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hh7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hh7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
-	[[Category: Ramesh, A.]]	+	[[Category: Ramesh, A]]
-	[[Category: Sacchettini,J C.]]	+	[[Category: Sacchettini,J C]]
	[[Category: 4-helix bundle]]		[[Category: 4-helix bundle]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 08:20, 30 September 2014

OCA — Tue, 30 Sep 2014 08:20:50 GMT

←Older revision		Revision as of 08:20, 30 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2hh7\| PDB=2hh7 \| SCENE= }}~~	+	==Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.==
-	===Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.===	+	<StructureSection load='2hh7' size='340' side='right' caption='[[2hh7]], [[Resolution\|resolution]] 2.55Å' scene=''>
-	~~{{ABSTRACT_PUBMED_17143269}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2hh7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HH7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hh7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hh7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hh7 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/2hh7_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Copper is an essential element that becomes highly cytotoxic when concentrations exceed the capacity of cells to sequester the ion. Here, we identify a new copper-specific repressor (CsoR) of a copper-sensitive operon (cso) in Mycobacterium tuberculosis (Mtb) that is representative of a large, previously uncharacterized family of proteins (DUF156). Electronic and X-ray absorption spectroscopies reveal that CsoR binds a single-monomer mole equivalent of Cu(I) to form a trigonally coordinated (S(2)N) Cu(I) complex. The 2.6-A crystal structure of copper-loaded CsoR shows a homodimeric antiparallel four-helix bundle architecture that represents a novel DNA-binding fold. The Cu(I) is coordinated by Cys36, Cys65' and His61' in a subunit bridging site. Cu(I) binding negatively regulates the binding of CsoR to a DNA fragment encompassing the operator-promoter region of the Mtb cso operon; this results in derepression of the operon in Mtb and the heterologous host Mycobacterium smegmatis. Substitution of Cys36 or His61 with alanine abolishes Cu(I)- and CsoR-dependent regulation in vivo and in vitro. Potential roles of CsoR in Mtb pathogenesis are discussed.

-	~~==About this Structure==~~	+	CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator.,Liu T, Ramesh A, Ma Z, Ward SK, Zhang L, George GN, Talaat AM, Sacchettini JC, Giedroc DP Nat Chem Biol. 2007 Jan;3(1):60-8. Epub 2006 Dec 3. PMID:17143269<ref>PMID:17143269</ref>
-	~~[[2hh7]]~~ is a 1 ~~chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=2HH7 OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
Line 10:		Line 29:
	*[[Molecular Playground/CsoR and RcnR\|Molecular Playground/CsoR and RcnR]]		*[[Molecular Playground/CsoR and RcnR\|Molecular Playground/CsoR and RcnR]]
	*[[Molecular Playground/Metal Responsive Regulators-Fall2001\|Molecular Playground/Metal Responsive Regulators-Fall2001]]		*[[Molecular Playground/Metal Responsive Regulators-Fall2001\|Molecular Playground/Metal Responsive Regulators-Fall2001]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:017143269<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Ramesh, A.]]		[[Category: Ramesh, A.]]

OCA at 09:36, 11 March 2013

OCA — Mon, 11 Mar 2013 09:36:59 GMT

←Older revision		Revision as of 09:36, 11 March 2013
Line 1:		Line 1:
-	[[Image:2hh7.png\|left\|200px]]
-
	{{STRUCTURE_2hh7\| PDB=2hh7 \| SCENE= }}		{{STRUCTURE_2hh7\| PDB=2hh7 \| SCENE= }}
-
	===Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.===		===Crystal Structure of Cu(I) bound CsoR from Mycobacterium tuberculosis.===
-
	{{ABSTRACT_PUBMED_17143269}}		{{ABSTRACT_PUBMED_17143269}}

OCA at 17:20, 20 October 2012

OCA — Sat, 20 Oct 2012 17:20:49 GMT

←Older revision		Revision as of 17:20, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2hh7]] is a 1 chain structure ~~of [[Copper homeostasis protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA].	+	[[2hh7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HH7 OCA].

	==See Also==		==See Also==

OCA: Protected "2hh7" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 00:37:02 GMT

Protected "2hh7" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:37, 27 July 2012