2hin - Revision history

OCA at 09:32, 14 February 2024

OCA — Wed, 14 Feb 2024 09:32:53 GMT

←Older revision		Revision as of 09:32, 14 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hin]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_N15 Escherichia virus N15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HIN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hin]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_N15 Escherichia virus N15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HIN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.05Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hin OCA], [https://pdbe.org/2hin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hin RCSB], [https://www.ebi.ac.uk/pdbsum/2hin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hin ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hin OCA], [https://pdbe.org/2hin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hin RCSB], [https://www.ebi.ac.uk/pdbsum/2hin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hin ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q37964_BPN15 Q37964_BPN15]		[https://www.uniprot.org/uniprot/Q37964_BPN15 Q37964_BPN15]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Bacteriophage Cro proteins bind to target DNA as dimers but do not all dimerize with equal strength, and differ in fold in the region of the dimer interface. We report the structure of the Cro protein from Enterobacteria phage N15 at 1.05 A resolution. The subunit fold contains five alpha-helices and is closely similar to the structure of P22 Cro (1.3 A backbone room mean square difference over 52 residues), but quite different from that of lambda Cro, a structurally diverged member of this family with a mixed alpha-helix/beta-sheet fold. N15 Cro crystallizes as a biological dimer with an extensive interface (1303 A(2) change in accessible surface area per dimer) and also dimerizes in solution with a K(d) of 5.1 +/- 1.5 microM. Its dimerization is much stronger than that of its structural homolog P22 Cro, which does not self-associate detectably in solution. Instead, the level of self-association and interfacial area for N15 Cro is similar to that of lambda Cro, even though these two orthologs do not share the same fold and have dimer interfaces that are qualitatively different in structure. The common Cro ancestor is thought to be an all-helical monomer similar to P22 Cro. We propose that two Cro descendants independently developed stronger dimerization by entirely different mechanisms.
-
-	N15 Cro and lambda Cro: orthologous DNA-binding domains with completely different but equally effective homodimer interfaces.,Dubrava MS, Ingram WM, Roberts SA, Weichsel A, Montfort WR, Cordes MH Protein Sci. 2008 May;17(5):803-12. Epub 2008 Mar 27. PMID:18369196<ref>PMID:18369196</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2hin" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:26, 21 December 2022

OCA — Wed, 21 Dec 2022 10:26:37 GMT

←Older revision		Revision as of 10:26, 21 December 2022
Line 3:		Line 3:
	<StructureSection load='2hin' size='340' side='right'caption='[[2hin]], [[Resolution\|resolution]] 1.05Å' scene=''>		<StructureSection load='2hin' size='340' side='right'caption='[[2hin]], [[Resolution\|resolution]] 1.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hin]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bpn15 Bpn15~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hin]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_N15 Escherichia virus N15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HIN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cro (gene 39) ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40631 BPN15])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hin OCA], [https://pdbe.org/2hin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hin RCSB], [https://www.ebi.ac.uk/pdbsum/2hin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hin ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hin OCA], [https://pdbe.org/2hin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hin RCSB], [https://www.ebi.ac.uk/pdbsum/2hin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hin ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q37964_BPN15 Q37964_BPN15]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bpn15~~]]	+	[[Category: Escherichia virus N15]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cordes~~, M H~~]]	+	[[Category: Cordes MH]]
-	[[Category: Dubrava~~, M S~~]]	+	[[Category: Dubrava MS]]
-	[[Category: Ingram~~, W M~~]]	+	[[Category: Ingram WM]]
-	[[Category: Montfort~~, W R~~]]	+	[[Category: Montfort WR]]
-	[[Category: Roberts~~, S A~~]]	+	[[Category: Roberts SA]]
-	[[Category: Weichsel, A]]	+	[[Category: Weichsel A]]
-	~~[[Category: Dimer interface]]~~	+
-	~~[[Category: Helix-turn-helix]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription factor~~]]	+

OCA at 07:41, 17 March 2021

OCA — Wed, 17 Mar 2021 07:41:06 GMT

←Older revision		Revision as of 07:41, 17 March 2021
Line 1:		Line 1:

	==Structure of N15 Cro at 1.05 A: an ortholog of lambda Cro with a completely different but equally effective dimerization mechanism==		==Structure of N15 Cro at 1.05 A: an ortholog of lambda Cro with a completely different but equally effective dimerization mechanism==
-	<StructureSection load='2hin' size='340' side='right' caption='[[2hin]], [[Resolution\|resolution]] 1.05Å' scene=''>	+	<StructureSection load='2hin' size='340' side='right'caption='[[2hin]], [[Resolution\|resolution]] 1.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hin]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bpn15 Bpn15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hin]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpn15 Bpn15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HIN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cro (gene 39) ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40631 BPN15])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cro (gene 39) ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40631 BPN15])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2hin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hin OCA], [~~http~~://pdbe.org/2hin PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2hin RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2hin PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2hin ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hin OCA], [https://pdbe.org/2hin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hin RCSB], [https://www.ebi.ac.uk/pdbsum/2hin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hin ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Bpn15]]		[[Category: Bpn15]]
		+	[[Category: Large Structures]]
	[[Category: Cordes, M H]]		[[Category: Cordes, M H]]
	[[Category: Dubrava, M S]]		[[Category: Dubrava, M S]]

OCA at 06:58, 27 June 2018

OCA — Wed, 27 Jun 2018 06:58:31 GMT

←Older revision		Revision as of 06:58, 27 June 2018
Line 1:		Line 1:
		+
	==Structure of N15 Cro at 1.05 A: an ortholog of lambda Cro with a completely different but equally effective dimerization mechanism==		==Structure of N15 Cro at 1.05 A: an ortholog of lambda Cro with a completely different but equally effective dimerization mechanism==
	<StructureSection load='2hin' size='340' side='right' caption='[[2hin]], [[Resolution\|resolution]] 1.05Å' scene=''>		<StructureSection load='2hin' size='340' side='right' caption='[[2hin]], [[Resolution\|resolution]] 1.05Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cro (gene 39) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40631 BPN15])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cro (gene 39) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40631 BPN15])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hin OCA], [http://pdbe.org/2hin PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hin RCSB], [http://www.ebi.ac.uk/pdbsum/2hin PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hin OCA], [http://pdbe.org/2hin PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hin RCSB], [http://www.ebi.ac.uk/pdbsum/2hin PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hin ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:22, 11 September 2015

OCA — Fri, 11 Sep 2015 07:22:50 GMT

←Older revision		Revision as of 07:22, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2hin' size='340' side='right' caption='[[2hin]], [[Resolution\|resolution]] 1.05Å' scene=''>		<StructureSection load='2hin' size='340' side='right' caption='[[2hin]], [[Resolution\|resolution]] 1.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hin]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_n15 Enterobacteria phage n15~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hin]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpn15 Bpn15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HIN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cro (gene 39) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40631 ~~Enterobacteria phage N15~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cro (gene 39) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40631 BPN15])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hin OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hin RCSB], [http://www.ebi.ac.uk/pdbsum/2hin PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hin OCA], [http://pdbe.org/2hin PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hin RCSB], [http://www.ebi.ac.uk/pdbsum/2hin PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2hin" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage n15~~]]	+	[[Category: Bpn15]]
	[[Category: Cordes, M H]]		[[Category: Cordes, M H]]
	[[Category: Dubrava, M S]]		[[Category: Dubrava, M S]]

OCA at 09:34, 16 January 2015

OCA — Fri, 16 Jan 2015 09:34:13 GMT

←Older revision		Revision as of 09:34, 16 January 2015
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage n15]]		[[Category: Enterobacteria phage n15]]
-	[[Category: Cordes, M H.]]	+	[[Category: Cordes, M H]]
-	[[Category: Dubrava, M S.]]	+	[[Category: Dubrava, M S]]
-	[[Category: Ingram, W M.]]	+	[[Category: Ingram, W M]]
-	[[Category: Montfort, W R.]]	+	[[Category: Montfort, W R]]
-	[[Category: Roberts, S A.]]	+	[[Category: Roberts, S A]]
-	[[Category: Weichsel, A.]]	+	[[Category: Weichsel, A]]
	[[Category: Dimer interface]]		[[Category: Dimer interface]]
	[[Category: Helix-turn-helix]]		[[Category: Helix-turn-helix]]
	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcription factor]]		[[Category: Transcription factor]]

OCA at 15:40, 12 October 2014

OCA — Sun, 12 Oct 2014 15:40:49 GMT

←Older revision		Revision as of 15:40, 12 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2hin.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of N15 Cro at 1.05 A: an ortholog of lambda Cro with a completely different but equally effective dimerization mechanism==
		+	<StructureSection load='2hin' size='340' side='right' caption='[[2hin]], [[Resolution\|resolution]] 1.05Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2hin]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_n15 Enterobacteria phage n15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HIN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cro (gene 39) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=40631 Enterobacteria phage N15])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hin OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hin RCSB], [http://www.ebi.ac.uk/pdbsum/2hin PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteriophage Cro proteins bind to target DNA as dimers but do not all dimerize with equal strength, and differ in fold in the region of the dimer interface. We report the structure of the Cro protein from Enterobacteria phage N15 at 1.05 A resolution. The subunit fold contains five alpha-helices and is closely similar to the structure of P22 Cro (1.3 A backbone room mean square difference over 52 residues), but quite different from that of lambda Cro, a structurally diverged member of this family with a mixed alpha-helix/beta-sheet fold. N15 Cro crystallizes as a biological dimer with an extensive interface (1303 A(2) change in accessible surface area per dimer) and also dimerizes in solution with a K(d) of 5.1 +/- 1.5 microM. Its dimerization is much stronger than that of its structural homolog P22 Cro, which does not self-associate detectably in solution. Instead, the level of self-association and interfacial area for N15 Cro is similar to that of lambda Cro, even though these two orthologs do not share the same fold and have dimer interfaces that are qualitatively different in structure. The common Cro ancestor is thought to be an all-helical monomer similar to P22 Cro. We propose that two Cro descendants independently developed stronger dimerization by entirely different mechanisms.

-	~~{{STRUCTURE_2hin\| PDB=2hin \| SCENE= }}~~	+	N15 Cro and lambda Cro: orthologous DNA-binding domains with completely different but equally effective homodimer interfaces.,Dubrava MS, Ingram WM, Roberts SA, Weichsel A, Montfort WR, Cordes MH Protein Sci. 2008 May;17(5):803-12. Epub 2008 Mar 27. PMID:18369196<ref>PMID:18369196</ref>

-	~~===Structure~~ of ~~N15 Cro at 1~~.~~05 A: an ortholog~~ of ~~lambda Cro with a completely different but equally effective dimerization mechanism===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18369196}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[2hin]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Enterobacteria_phage_n15 Enterobacteria phage n15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIN OCA].~~	+	</StructureSection>
	[[Category: Enterobacteria phage n15]]		[[Category: Enterobacteria phage n15]]
	[[Category: Cordes, M H.]]		[[Category: Cordes, M H.]]

OCA: Protected "2hin" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 12:46:53 GMT

Protected "2hin" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:46, 7 January 2013

OCA at 12:46, 7 January 2013

OCA — Mon, 07 Jan 2013 12:46:50 GMT

←Older revision		Revision as of 12:46, 7 January 2013
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-	{{Seed}}
	[[Image:2hin.png\|left\|200px]]		[[Image:2hin.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2hin\| PDB=2hin \| SCENE= }}		{{STRUCTURE_2hin\| PDB=2hin \| SCENE= }}

	===Structure of N15 Cro at 1.05 A: an ortholog of lambda Cro with a completely different but equally effective dimerization mechanism===		===Structure of N15 Cro at 1.05 A: an ortholog of lambda Cro with a completely different but equally effective dimerization mechanism===

-
-	<!--
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	{{ABSTRACT_PUBMED_18369196}}		{{ABSTRACT_PUBMED_18369196}}

	==About this Structure==		==About this Structure==
-	~~2HIN is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_n15 Enterobacteria phage n15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIN OCA].	+	[[2hin]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_n15 Enterobacteria phage n15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIN OCA].
-		+
-	~~==Reference==~~	+
-	N15 Cro and lambda Cro: orthologous DNA-binding domains with completely different but equally effective homodimer interfaces., Dubrava MS, Ingram WM, Roberts SA, Weichsel A, Montfort WR, Cordes MH, Protein Sci. 2008 May;17(5):803-12. Epub 2008 Mar 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18369196 18369196]	+
	[[Category: Enterobacteria phage n15]]		[[Category: Enterobacteria phage n15]]
-	[[Category: Single protein]]
	[[Category: Cordes, M H.]]		[[Category: Cordes, M H.]]
	[[Category: Dubrava, M S.]]		[[Category: Dubrava, M S.]]
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	[[Category: Dimer interface]]		[[Category: Dimer interface]]
	[[Category: Helix-turn-helix]]		[[Category: Helix-turn-helix]]
		+	[[Category: Transcription]]
	[[Category: Transcription factor]]		[[Category: Transcription factor]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:54:20 2008''

OCA at 20:54, 28 July 2008

OCA — Mon, 28 Jul 2008 20:54:21 GMT

←Older revision		Revision as of 20:54, 28 July 2008
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-	[[Image:2hin.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2hin.png\|left\|200px]]

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	{{STRUCTURE_2hin\| PDB=2hin \| SCENE= }}		{{STRUCTURE_2hin\| PDB=2hin \| SCENE= }}

-	~~'''~~Structure of N15 Cro at 1.05 A: an ortholog of lambda Cro with a completely different but equally effective dimerization mechanism~~'''~~	+	===Structure of N15 Cro at 1.05 A: an ortholog of lambda Cro with a completely different but equally effective dimerization mechanism===


-	~~==Overview==~~	+	<!--
-	~~Bacteriophage Cro proteins bind to target DNA as dimers but do not all dimerize with equal strength~~, ~~and differ in fold in~~ the ~~region of the dimer interface. We report the structure of the Cro protein from Enterobacteria phage N15 at 1.05 A resolution. The subunit fold contains five alpha-helices and is closely similar~~ to the ~~structure of P22 Cro~~ (1.3 A backbone room mean square difference over 52 residues), but quite different from that of lambda Cro, a structurally diverged member of this family with a mixed alpha-helix/beta-sheet fold. N15 Cro crystallizes as ~~a biological dimer with an extensive interface (1303 A(2) change in accessible surface area per dimer) and also dimerizes in solution with a K(d) of 5.1 +~~/~~- 1~~.~~5 microM~~. ~~Its dimerization is much stronger than that of its structural homolog P22 Cro~~, ~~which does not self-associate detectably in solution. Instead, the level of self-association and interfacial area for N15 Cro~~ is ~~similar to that of lambda Cro, even though these two orthologs do not share~~ the ~~same fold and have dimer interfaces that are qualitatively different in structure~~. ~~The common Cro ancestor is thought to be an all~~-~~helical monomer similar to P22 Cro. We propose that two Cro descendants independently developed stronger dimerization by entirely different mechanisms.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_18369196}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18369196 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18369196}}

	==About this Structure==		==About this Structure==
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	[[Category: Helix-turn-helix]]		[[Category: Helix-turn-helix]]
	[[Category: Transcription factor]]		[[Category: Transcription factor]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu May 22 22~~:24:33 2008''	+
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