2hk0 - Revision history

OCA at 10:03, 12 May 2021

OCA — Wed, 12 May 2021 10:03:23 GMT

←Older revision		Revision as of 10:03, 12 May 2021
Line 3:		Line 3:
	<StructureSection load='2hk0' size='340' side='right'caption='[[2hk0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2hk0' size='340' side='right'caption='[[2hk0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hk0]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"achromobacter_radiobacter"_(beijerinck_and_van_delden_1902)_bergey_et_al._1934 "achromobacter radiobacter" (beijerinck and van delden 1902) bergey et al. 1934]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HK0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hk0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"achromobacter_radiobacter"_(beijerinck_and_van_delden_1902)_bergey_et_al._1934 "achromobacter radiobacter" (beijerinck and van delden 1902) bergey et al. 1934]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HK0 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hk1\|2hk1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2hk1\|2hk1]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2hk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hk0 OCA], [~~http~~://pdbe.org/2hk0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2hk0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2hk0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2hk0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hk0 OCA], [https://pdbe.org/2hk0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hk0 RCSB], [https://www.ebi.ac.uk/pdbsum/2hk0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hk0 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 09:02, 24 July 2019

OCA — Wed, 24 Jul 2019 09:02:23 GMT

←Older revision		Revision as of 09:02, 24 July 2019
Line 1:		Line 1:

	==Crystal structure of D-psicose 3-epimerase (DPEase) in the absence of substrate==		==Crystal structure of D-psicose 3-epimerase (DPEase) in the absence of substrate==
-	<StructureSection load='2hk0' size='340' side='right' caption='[[2hk0]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='2hk0' size='340' side='right'caption='[[2hk0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hk0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_radiobacter"_(beijerinck_and_van_delden_1902)_bergey_et_al._1934 "achromobacter radiobacter" (beijerinck and van delden 1902) bergey et al. 1934]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HK0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hk0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_radiobacter"_(beijerinck_and_van_delden_1902)_bergey_et_al._1934 "achromobacter radiobacter" (beijerinck and van delden 1902) bergey et al. 1934]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HK0 FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/2hk0_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/2hk0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Cha, S S]]		[[Category: Cha, S S]]
	[[Category: Kim, H J]]		[[Category: Kim, H J]]

OCA at 08:13, 18 October 2017

OCA — Wed, 18 Oct 2017 08:13:39 GMT

←Older revision		Revision as of 08:13, 18 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of D-psicose 3-epimerase (DPEase) in the absence of substrate==		==Crystal structure of D-psicose 3-epimerase (DPEase) in the absence of substrate==
	<StructureSection load='2hk0' size='340' side='right' caption='[[2hk0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2hk0' size='340' side='right' caption='[[2hk0]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hk1\|2hk1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hk1\|2hk1]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hk0 OCA], [http://pdbe.org/2hk0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hk0 RCSB], [http://www.ebi.ac.uk/pdbsum/2hk0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hk0 OCA], [http://pdbe.org/2hk0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hk0 RCSB], [http://www.ebi.ac.uk/pdbsum/2hk0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hk0 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hk0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:30, 11 September 2015

OCA — Fri, 11 Sep 2015 10:30:16 GMT

←Older revision		Revision as of 10:30, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2hk0' size='340' side='right' caption='[[2hk0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2hk0' size='340' side='right' caption='[[2hk0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hk0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Agrobacterium_tumefaciens Agrobacterium tumefaciens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HK0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hk0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_radiobacter"_(beijerinck_and_van_delden_1902)_bergey_et_al._1934 "achromobacter radiobacter" (beijerinck and van delden 1902) bergey et al. 1934]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HK0 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hk1\|2hk1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hk1\|2hk1]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hk0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hk0 RCSB], [http://www.ebi.ac.uk/pdbsum/2hk0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hk0 OCA], [http://pdbe.org/2hk0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hk0 RCSB], [http://www.ebi.ac.uk/pdbsum/2hk0 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2hk0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Agrobacterium tumefaciens]]
	[[Category: Cha, S S]]		[[Category: Cha, S S]]
	[[Category: Kim, H J]]		[[Category: Kim, H J]]

OCA at 08:04, 16 January 2015

OCA — Fri, 16 Jan 2015 08:04:19 GMT

←Older revision		Revision as of 08:04, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hk0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HK0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hk0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HK0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hk1\|2hk1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hk1\|2hk1]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hk0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hk0 RCSB], [http://www.ebi.ac.uk/pdbsum/2hk0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hk0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hk0 RCSB], [http://www.ebi.ac.uk/pdbsum/2hk0 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Agrobacterium tumefaciens]]		[[Category: Agrobacterium tumefaciens]]
-	[[Category: Cha, S S.]]	+	[[Category: Cha, S S]]
-	[[Category: Kim, H J.]]	+	[[Category: Kim, H J]]
-	[[Category: Kim, K.]]	+	[[Category: Kim, K]]
-	[[Category: Oh, D K.]]	+	[[Category: Oh, D K]]
-	[[Category: Rhee, S.]]	+	[[Category: Rhee, S]]
	[[Category: Isomerase]]		[[Category: Isomerase]]
	[[Category: Tim-barrel]]		[[Category: Tim-barrel]]

OCA at 07:46, 30 September 2014

OCA — Tue, 30 Sep 2014 07:46:06 GMT

←Older revision		Revision as of 07:46, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2hk0.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of D-psicose 3-epimerase (DPEase) in the absence of substrate==
		+	<StructureSection load='2hk0' size='340' side='right' caption='[[2hk0]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2hk0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HK0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hk1\|2hk1]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hk0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hk0 RCSB], [http://www.ebi.ac.uk/pdbsum/2hk0 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/2hk0_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	D-psicose, a rare sugar produced by the enzymatic reaction of D-tagatose 3-epimerase (DTEase), has been used extensively for the bioproduction of various rare carbohydrates. Recently characterized D-psicose 3-epimerase (DPEase) from Agrobacterium tumefaciens was found to belong to the DTEase family and to catalyze the interconversion of D-fructose and D-psicose by epimerizing the C-3 position, with marked efficiency for D-psicose. The crystal structures of DPEase and its complex with the true substrate D-fructose were determined; DPEase is a tetramer and each monomer belongs to a TIM-barrel fold. The active site in each subunit is distinct from that of other TIM-barrel enzymes, which use phosphorylated ligands as the substrate. It contains a metal ion with octahedral coordination to two water molecules and four residues that are absolutely conserved across the DTEase family. Upon binding of D-fructose, the substrate displaces water molecules in the active site, with a conformation mimicking the intermediate cis-enediolate. Subsequently, Trp112 and Pro113 in the beta4-alpha4 loop undergo significant structural changes, sealing off the active site. Structural evidence and site-directed mutagenesis of the putative catalytic residues suggest that the metal ion plays a pivotal role in catalysis by anchoring the bound D-fructose, and Glu150 and Glu244 carry out an epimerization reaction at the C-3 position.

-	~~{{STRUCTURE_2hk0\| PDB=2hk0 \| SCENE= }}~~	+	Crystal structure of D-psicose 3-epimerase from Agrobacterium tumefaciens and its complex with true substrate D-fructose: a pivotal role of metal in catalysis, an active site for the non-phosphorylated substrate, and its conformational changes.,Kim K, Kim HJ, Oh DK, Cha SS, Rhee S J Mol Biol. 2006 Sep 1;361(5):920-31. Epub 2006 Jul 28. PMID:16876192<ref>PMID:16876192</ref>

-	~~===Crystal structure~~ of ~~D-psicose 3-epimerase (DPEase) in~~ the ~~absence of substrate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16876192}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2hk0]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016876192<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Agrobacterium tumefaciens]]		[[Category: Agrobacterium tumefaciens]]
	[[Category: Cha, S S.]]		[[Category: Cha, S S.]]

OCA: Protected "2hk0" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 10:19:38 GMT

Protected "2hk0" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:19, 7 January 2013

OCA at 10:19, 7 January 2013

OCA — Mon, 07 Jan 2013 10:19:34 GMT

←Older revision		Revision as of 10:19, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2hk0.png\|left\|200px]]		[[Image:2hk0.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2hk0", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2hk0\| PDB=2hk0 \| SCENE= }}		{{STRUCTURE_2hk0\| PDB=2hk0 \| SCENE= }}

	===Crystal structure of D-psicose 3-epimerase (DPEase) in the absence of substrate===		===Crystal structure of D-psicose 3-epimerase (DPEase) in the absence of substrate===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16876192}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16876192 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16876192}}		{{ABSTRACT_PUBMED_16876192}}

	==About this Structure==		==About this Structure==
-	~~2HK0~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA].	+	[[2hk0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16876192~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016876192</ref><references group="xtra"/>
	[[Category: Agrobacterium tumefaciens]]		[[Category: Agrobacterium tumefaciens]]
	[[Category: Cha, S S.]]		[[Category: Cha, S S.]]
Line 30:		Line 18:
	[[Category: Oh, D K.]]		[[Category: Oh, D K.]]
	[[Category: Rhee, S.]]		[[Category: Rhee, S.]]
		+	[[Category: Isomerase]]
	[[Category: Tim-barrel]]		[[Category: Tim-barrel]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:56:25 2009''

OCA at 17:56, 17 February 2009

OCA — Tue, 17 Feb 2009 17:56:26 GMT

←Older revision		Revision as of 17:56, 17 February 2009
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	==About this Structure==		==About this Structure==
-	2HK0 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA].	+	2HK0 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HK0 OCA].

	==Reference==		==Reference==
-	Crystal structure of D-psicose 3-epimerase from Agrobacterium tumefaciens and its complex with true substrate D-fructose: a pivotal role of metal in catalysis, an active site for the non-phosphorylated substrate, and its conformational changes., Kim K, Kim HJ, Oh DK, Cha SS, Rhee S, J Mol Biol. 2006 Sep 1;361(5):920-31. Epub 2006 Jul 28. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16876192 16876192]~~	+	<ref group="xtra">PMID:16876192</ref><references group="xtra"/>
	[[Category: Agrobacterium tumefaciens]]		[[Category: Agrobacterium tumefaciens]]
-	[[Category: Single protein]]
	[[Category: Cha, S S.]]		[[Category: Cha, S S.]]
	[[Category: Kim, H J.]]		[[Category: Kim, H J.]]
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	[[Category: Tim-barrel]]		[[Category: Tim-barrel]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 03~~:50:~~33 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:56:25 2009''

OCA at 00:50, 29 July 2008

OCA — Tue, 29 Jul 2008 00:50:35 GMT

←Older revision		Revision as of 00:50, 29 July 2008
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-	[[Image:2hk0.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2hk0.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2hk0\| PDB=2hk0 \| SCENE= }}		{{STRUCTURE_2hk0\| PDB=2hk0 \| SCENE= }}

-	~~'''~~Crystal structure of D-psicose 3-epimerase (DPEase) in the absence of substrate~~'''~~	+	===Crystal structure of D-psicose 3-epimerase (DPEase) in the absence of substrate===


-	~~==Overview==~~	+	<!--
-	D-~~psicose, a rare sugar produced by the enzymatic reaction of D~~-~~tagatose 3-epimerase (DTEase)~~, ~~has been used extensively for~~ the ~~bioproduction of various rare carbohydrates. Recently characterized D-psicose 3-epimerase (DPEase) from Agrobacterium tumefaciens was found to belong~~ to the DTEase family and to catalyze the interconversion of D-fructose and D-psicose by epimerizing the C-3 position, with marked efficiency for D-psicose. The crystal structures of DPEase and its complex with the true substrate D-fructose were determined; DPEase is a tetramer and each monomer belongs to a TIM-barrel fold. The active site in each subunit is distinct from that of other TIM-barrel enzymes, which use phosphorylated ligands as ~~the substrate~~. ~~It contains a metal ion with octahedral coordination to two water molecules and four residues that are absolutely conserved across the DTEase family~~. ~~Upon binding of D-fructose~~, the ~~substrate displaces water molecules in the active site, with a conformation mimicking the intermediate cis-enediolate~~. ~~Subsequently, Trp112 and Pro113 in the beta4~~-~~alpha4 loop undergo significant structural changes, sealing off the active site. Structural evidence and site~~-directed mutagenesis of the putative catalytic residues suggest that the metal ion plays a pivotal role in catalysis by anchoring the bound D-fructose, and Glu150 and Glu244 carry out an epimerization reaction at the C-3 position.	+	The line below this paragraph, {{ABSTRACT_PUBMED_16876192}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16876192 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16876192}}

	==About this Structure==		==About this Structure==
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	[[Category: Rhee, S.]]		[[Category: Rhee, S.]]
	[[Category: Tim-barrel]]		[[Category: Tim-barrel]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 06~~:22:58 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:50:33 2008''