2hos - Revision history

OCA at 10:00, 30 August 2023

OCA — Wed, 30 Aug 2023 10:00:05 GMT

←Older revision		Revision as of 10:00, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='2hos' size='340' side='right'caption='[[2hos]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2hos' size='340' side='right'caption='[[2hos]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hos]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HOS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hos]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HOS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=3MO:3-METHYL~~-1~~,3-OXAZOLIDIN-2-ONE'>3MO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2hdd\|2hdd]], [[2hot\|2hot]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3MO:3-METHYL-1,3-OXAZOLIDIN-2-ONE'>3MO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">en ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hos OCA], [https://pdbe.org/2hos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hos RCSB], [https://www.ebi.ac.uk/pdbsum/2hos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hos ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hos OCA], [https://pdbe.org/2hos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hos RCSB], [https://www.ebi.ac.uk/pdbsum/2hos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hos ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HMEN_DROME HMEN_DROME]] This protein specifies the body segmentation pattern. It is required for the development of the central nervous system. Transcriptional regulator that represses activated promoters. Wg signaling operates by inactivating the SGG repression of EN autoactivation.	+	[https://www.uniprot.org/uniprot/HMEN_DROME HMEN_DROME] This protein specifies the body segmentation pattern. It is required for the development of the central nervous system. Transcriptional regulator that represses activated promoters. Wg signaling operates by inactivating the SGG repression of EN autoactivation.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drome~~]]	+	[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Feldman~~, M E~~]]	+	[[Category: Feldman ME]]
-	[[Category: Shokat~~, K M~~]]	+	[[Category: Shokat KM]]
-	[[Category: Simon~~, M D]]~~	+	[[Category: Simon MD]]
-	~~[[Category: Homeodomain]]~~	+
-	~~[[Category: Phage display]]~~	+
-	~~[[Category: Transcription-dna complex~~]]	+

OCA at 07:19, 24 March 2021

OCA — Wed, 24 Mar 2021 07:19:02 GMT

←Older revision		Revision as of 07:19, 24 March 2021
Line 1:		Line 1:

	==Phage-Selected Homeodomain Bound to Unmodified DNA==		==Phage-Selected Homeodomain Bound to Unmodified DNA==
-	<StructureSection load='2hos' size='340' side='right' caption='[[2hos]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='2hos' size='340' side='right'caption='[[2hos]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hos]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HOS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hos]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HOS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3MO:3-METHYL-1,3-OXAZOLIDIN-2-ONE'>3MO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3MO:3-METHYL-1,3-OXAZOLIDIN-2-ONE'>3MO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hdd\|2hdd]], [[2hot\|2hot]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2hdd\|2hdd]], [[2hot\|2hot]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">en ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">en ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hos OCA], [~~http~~://pdbe.org/2hos PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2hos RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2hos PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2hos ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hos OCA], [https://pdbe.org/2hos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hos RCSB], [https://www.ebi.ac.uk/pdbsum/2hos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hos ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HMEN_DROME HMEN_DROME]] This protein specifies the body segmentation pattern. It is required for the development of the central nervous system. Transcriptional regulator that represses activated promoters. Wg signaling operates by inactivating the SGG repression of EN autoactivation.	+	[[https://www.uniprot.org/uniprot/HMEN_DROME HMEN_DROME]] This protein specifies the body segmentation pattern. It is required for the development of the central nervous system. Transcriptional regulator that represses activated promoters. Wg signaling operates by inactivating the SGG repression of EN autoactivation.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ho/2hos_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ho/2hos_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Drome]]		[[Category: Drome]]
		+	[[Category: Large Structures]]
	[[Category: Feldman, M E]]		[[Category: Feldman, M E]]
	[[Category: Shokat, K M]]		[[Category: Shokat, K M]]

OCA at 08:14, 18 October 2017

OCA — Wed, 18 Oct 2017 08:14:59 GMT

←Older revision		Revision as of 08:14, 18 October 2017
Line 1:		Line 1:
		+
	==Phage-Selected Homeodomain Bound to Unmodified DNA==		==Phage-Selected Homeodomain Bound to Unmodified DNA==
	<StructureSection load='2hos' size='340' side='right' caption='[[2hos]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2hos' size='340' side='right' caption='[[2hos]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hdd\|2hdd]], [[2hot\|2hot]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hdd\|2hdd]], [[2hot\|2hot]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">en ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">en ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hos OCA], [http://pdbe.org/2hos PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hos RCSB], [http://www.ebi.ac.uk/pdbsum/2hos PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hos OCA], [http://pdbe.org/2hos PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hos RCSB], [http://www.ebi.ac.uk/pdbsum/2hos PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hos ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 16:28, 8 February 2016

OCA — Mon, 08 Feb 2016 16:28:28 GMT

←Older revision		Revision as of 16:28, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hos ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:23, 10 September 2015

OCA — Thu, 10 Sep 2015 13:23:48 GMT

←Older revision		Revision as of 13:23, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2hos' size='340' side='right' caption='[[2hos]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2hos' size='340' side='right' caption='[[2hos]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hos]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Drosophila_melanogaster Drosophila melanogaster~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HOS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hos]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HOS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3MO:3-METHYL-1,3-OXAZOLIDIN-2-ONE'>3MO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3MO:3-METHYL-1,3-OXAZOLIDIN-2-ONE'>3MO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hdd\|2hdd]], [[2hot\|2hot]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hdd\|2hdd]], [[2hot\|2hot]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">en ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 ~~Drosophila melanogaster~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">en ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hos OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hos RCSB], [http://www.ebi.ac.uk/pdbsum/2hos PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hos OCA], [http://pdbe.org/2hos PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hos RCSB], [http://www.ebi.ac.uk/pdbsum/2hos PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2hos" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drosophila melanogaster~~]]	+	[[Category: Drome]]
	[[Category: Feldman, M E]]		[[Category: Feldman, M E]]
	[[Category: Shokat, K M]]		[[Category: Shokat, K M]]

OCA at 00:39, 25 December 2014

OCA — Thu, 25 Dec 2014 00:39:03 GMT

←Older revision		Revision as of 00:39, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hos]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HOS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hos]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HOS FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3MO:3-METHYL-1,3-OXAZOLIDIN-2-ONE'>3MO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3MO:3-METHYL-1,3-OXAZOLIDIN-2-ONE'>3MO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hdd\|2hdd]], [[2hot\|2hot]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hdd\|2hdd]], [[2hot\|2hot]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">en ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">en ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hos OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hos RCSB], [http://www.ebi.ac.uk/pdbsum/2hos PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hos OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hos RCSB], [http://www.ebi.ac.uk/pdbsum/2hos PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HMEN_DROME HMEN_DROME]] This protein specifies the body segmentation pattern. It is required for the development of the central nervous system. Transcriptional regulator that represses activated promoters. Wg signaling operates by inactivating the SGG repression of EN autoactivation.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
-	[[Category: Feldman, M E.]]	+	[[Category: Feldman, M E]]
-	[[Category: Shokat, K M.]]	+	[[Category: Shokat, K M]]
-	[[Category: Simon, M D.]]	+	[[Category: Simon, M D]]
	[[Category: Homeodomain]]		[[Category: Homeodomain]]
	[[Category: Phage display]]		[[Category: Phage display]]
	[[Category: Transcription-dna complex]]		[[Category: Transcription-dna complex]]

OCA at 07:52, 30 September 2014

OCA — Tue, 30 Sep 2014 07:52:26 GMT

←Older revision		Revision as of 07:52, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2hos.~~png~~\|~~left~~\|200px]]	+	==Phage-Selected Homeodomain Bound to Unmodified DNA==
		+	<StructureSection load='2hos' size='340' side='right' caption='[[2hos]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2hos]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HOS FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3MO:3-METHYL-1,3-OXAZOLIDIN-2-ONE'>3MO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hdd\|2hdd]], [[2hot\|2hot]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">en ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hos OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hos RCSB], [http://www.ebi.ac.uk/pdbsum/2hos PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ho/2hos_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The homeodomain (HD)-DNA interface has been conserved over 500 million years of evolution. Despite this conservation, we have successfully re-engineered the engrailed HD to specifically recognize an unnatural nucleotide using a phage display selection. Here we report the synthesis of novel nucleosides and the selection of mutant HDs that bind these nucleotides using phage display. The high-resolution crystal structure of one mutant in complex with modified and unmodified DNA demonstrates that, even with the substantial perturbation to the interface, this selected mutant retains a canonical HD structure. Dissection of the contributions due to each of the selected mutations reveals that the majority of the modification-specific binding is accomplished by a single mutation (I47G) but that the remaining mutations retune the stability of the HD. These results afford a detailed look at a re-engineered protein-DNA interaction and provide insight into the opportunities for re-engineering highly conserved interfaces.

-	~~{{STRUCTURE_2hos\| PDB=2hos \| SCENE= }}~~	+	Structure and properties of a re-engineered homeodomain protein-DNA interface.,Simon MD, Feldman ME, Rauh D, Maris AE, Wemmer DE, Shokat KM ACS Chem Biol. 2006 Dec 15;1(12):755-60. PMID:17240973<ref>PMID:17240973</ref>

-	~~===Phage-Selected Homeodomain Bound to Unmodified DNA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17240973}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2hos]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Drosophila_melanogaster Drosophila melanogaster]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017240973<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Feldman, M E.]]		[[Category: Feldman, M E.]]

OCA: Protected "2hos" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 12:59:52 GMT

Protected "2hos" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:59, 7 January 2013

OCA at 12:59, 7 January 2013

OCA — Mon, 07 Jan 2013 12:59:49 GMT

←Older revision		Revision as of 12:59, 7 January 2013
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-	{{Seed}}
	[[Image:2hos.png\|left\|200px]]		[[Image:2hos.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2hos\| PDB=2hos \| SCENE= }}		{{STRUCTURE_2hos\| PDB=2hos \| SCENE= }}

	===Phage-Selected Homeodomain Bound to Unmodified DNA===		===Phage-Selected Homeodomain Bound to Unmodified DNA===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17240973}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17240973 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17240973}}		{{ABSTRACT_PUBMED_17240973}}

	==About this Structure==		==About this Structure==
-	~~2HOS~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA].	+	[[2hos]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17240973~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017240973</ref><references group="xtra"/>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Feldman, M E.]]		[[Category: Feldman, M E.]]
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	[[Category: Homeodomain]]		[[Category: Homeodomain]]
	[[Category: Phage display]]		[[Category: Phage display]]
-		+	[[Category: Transcription-dna complex]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 00:26:37 2009''~~	+

OCA at 22:26, 16 February 2009

OCA — Mon, 16 Feb 2009 22:26:38 GMT

←Older revision		Revision as of 22:26, 16 February 2009
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	==About this Structure==		==About this Structure==
-	2HOS is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA].	+	2HOS is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HOS OCA].

	==Reference==		==Reference==
-	~~Structure and properties of a re-engineered homeodomain protein-DNA interface., Simon MD, Feldman ME, Rauh D, Maris AE, Wemmer DE, Shokat KM, ACS Chem Biol. 2006 Dec 15;1(12):755-60.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17240973 17240973]~~	+	<ref group="xtra">PMID:17240973</ref><references group="xtra"/>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
-	[[Category: Single protein]]
	[[Category: Feldman, M E.]]		[[Category: Feldman, M E.]]
	[[Category: Shokat, K M.]]		[[Category: Shokat, K M.]]
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	[[Category: Phage display]]		[[Category: Phage display]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 04~~:51:~~39 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:26:37 2009''