2hxu - Revision history

OCA at 10:03, 30 August 2023

OCA — Wed, 30 Aug 2023 10:03:19 GMT

←Older revision		Revision as of 10:03, 30 August 2023
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	<StructureSection load='2hxu' size='340' side='right'caption='[[2hxu]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2hxu' size='340' side='right'caption='[[2hxu]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hxu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Xance Xance~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HXU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hxu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris Xanthomonas campestris pv. campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HXU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2hne\|2hne]]</div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [https://pdbe.org/2hxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [https://www.ebi.ac.uk/pdbsum/2hxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hxu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [https://pdbe.org/2hxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [https://www.ebi.ac.uk/pdbsum/2hxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hxu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FUCD_XANCP FUCD_XANCP]] Plays a role in the catabolism of L-fucose. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion. L-fuconate is the preferred substrate with 15-fold lower activity observed for L-galactonate and 8-fold lower activity with D-arabinonate. No activity detected with D-fuconate. Can also catalyze the epimerization of L-talonate and D-ribonate, but at slow rates.<ref>PMID:17144652</ref>	+	[https://www.uniprot.org/uniprot/FUCD_XANCP FUCD_XANCP] Plays a role in the catabolism of L-fucose. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion. L-fuconate is the preferred substrate with 15-fold lower activity observed for L-galactonate and 8-fold lower activity with D-arabinonate. No activity detected with D-fuconate. Can also catalyze the epimerization of L-talonate and D-ribonate, but at slow rates.<ref>PMID:17144652</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Xance~~]]	+	[[Category: Xanthomonas campestris pv. campestris]]
-	[[Category: Almo~~, S C~~]]	+	[[Category: Almo SC]]
-	[[Category: Fedorov~~, A A~~]]	+	[[Category: Fedorov AA]]
-	[[Category: Fedorov~~, E V~~]]	+	[[Category: Fedorov EV]]
-	[[Category: Gerlt~~, J A~~]]	+	[[Category: Gerlt JA]]
-	[[Category: Rakus~~, J F~~]]	+	[[Category: Rakus JF]]
-	[[Category: Yew~~, W S]]~~	+	[[Category: Yew WS]]
-	~~[[Category: Enolase superfamily]]~~	+
-	~~[[Category: L-fuconate]]~~	+
-	~~[[Category: L-fuconate dehydratase]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 17:47, 20 October 2021

OCA — Wed, 20 Oct 2021 17:47:58 GMT

←Older revision		Revision as of 17:47, 20 October 2021
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	<StructureSection load='2hxu' size='340' side='right'caption='[[2hxu]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2hxu' size='340' side='right'caption='[[2hxu]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hxu]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Xance Xance]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2HXU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hxu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xance Xance]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HXU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hne\|2hne]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2hne\|2hne]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [~~http~~://pdbe.org/2hxu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2hxu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2hxu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [https://pdbe.org/2hxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [https://www.ebi.ac.uk/pdbsum/2hxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hxu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FUCD_XANCP FUCD_XANCP]] Plays a role in the catabolism of L-fucose. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion. L-fuconate is the preferred substrate with 15-fold lower activity observed for L-galactonate and 8-fold lower activity with D-arabinonate. No activity detected with D-fuconate. Can also catalyze the epimerization of L-talonate and D-ribonate, but at slow rates.<ref>PMID:17144652</ref>	+	[[https://www.uniprot.org/uniprot/FUCD_XANCP FUCD_XANCP]] Plays a role in the catabolism of L-fucose. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion. L-fuconate is the preferred substrate with 15-fold lower activity observed for L-galactonate and 8-fold lower activity with D-arabinonate. No activity detected with D-fuconate. Can also catalyze the epimerization of L-talonate and D-ribonate, but at slow rates.<ref>PMID:17144652</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:40, 8 April 2020

OCA — Wed, 08 Apr 2020 07:40:23 GMT

←Older revision		Revision as of 07:40, 8 April 2020
Line 1:		Line 1:

	==Crystal structure of K220A mutant of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and L-fuconate==		==Crystal structure of K220A mutant of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and L-fuconate==
-	<StructureSection load='2hxu' size='340' side='right' caption='[[2hxu]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='2hxu' size='340' side='right'caption='[[2hxu]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xance Xance]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HXU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xance Xance]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2HXU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hne\|2hne]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hne\|2hne]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [http://pdbe.org/2hxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [http://www.ebi.ac.uk/pdbsum/2hxu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hxu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [http://pdbe.org/2hxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [http://www.ebi.ac.uk/pdbsum/2hxu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hxu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Xance]]		[[Category: Xance]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]

OCA at 08:02, 4 July 2018

OCA — Wed, 04 Jul 2018 08:02:13 GMT

←Older revision		Revision as of 08:02, 4 July 2018
Line 1:		Line 1:
		+
	==Crystal structure of K220A mutant of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and L-fuconate==		==Crystal structure of K220A mutant of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and L-fuconate==
	<StructureSection load='2hxu' size='340' side='right' caption='[[2hxu]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2hxu' size='340' side='right' caption='[[2hxu]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hne\|2hne]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hne\|2hne]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [http://pdbe.org/2hxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [http://www.ebi.ac.uk/pdbsum/2hxu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [http://pdbe.org/2hxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [http://www.ebi.ac.uk/pdbsum/2hxu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hxu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hx/2hxu_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hx/2hxu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hxu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:24, 11 September 2015

OCA — Fri, 11 Sep 2015 14:24:56 GMT

←Older revision		Revision as of 14:24, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2hxu' size='340' side='right' caption='[[2hxu]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2hxu' size='340' side='right' caption='[[2hxu]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Xanthomonas_campestris_pv._campestris Xanthomonas campestris pv. campestris~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HXU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xance Xance]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HXU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hne\|2hne]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hne\|2hne]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [http://www.ebi.ac.uk/pdbsum/2hxu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [http://pdbe.org/2hxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [http://www.ebi.ac.uk/pdbsum/2hxu PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FUCD_XANCP FUCD_XANCP]] Plays a role in the catabolism of L-fucose. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion. L-fuconate is the preferred substrate with 15-fold lower activity observed for L-galactonate and 8-fold lower activity with D-arabinonate. No activity detected with D-fuconate. Can also catalyze the epimerization of L-talonate and D-ribonate, but at slow rates.<ref>PMID:17144652</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 25:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2hxu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Xanthomonas campestris pv. campestris~~]]	+	[[Category: Xance]]
	[[Category: Almo, S C]]		[[Category: Almo, S C]]
	[[Category: Fedorov, A A]]		[[Category: Fedorov, A A]]

OCA at 17:40, 25 December 2014

OCA — Thu, 25 Dec 2014 17:40:57 GMT

←Older revision		Revision as of 17:40, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris Xanthomonas campestris pv. campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HXU FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris Xanthomonas campestris pv. campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HXU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hne\|2hne]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hne\|2hne]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [http://www.ebi.ac.uk/pdbsum/2hxu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [http://www.ebi.ac.uk/pdbsum/2hxu PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Xanthomonas campestris pv. campestris]]		[[Category: Xanthomonas campestris pv. campestris]]
-	[[Category: Almo, S C.]]	+	[[Category: Almo, S C]]
-	[[Category: Fedorov, A A.]]	+	[[Category: Fedorov, A A]]
-	[[Category: Fedorov, E V.]]	+	[[Category: Fedorov, E V]]
-	[[Category: Gerlt, J A.]]	+	[[Category: Gerlt, J A]]
-	[[Category: Rakus, J F.]]	+	[[Category: Rakus, J F]]
-	[[Category: Yew, W S.]]	+	[[Category: Yew, W S]]
	[[Category: Enolase superfamily]]		[[Category: Enolase superfamily]]
	[[Category: L-fuconate]]		[[Category: L-fuconate]]
	[[Category: L-fuconate dehydratase]]		[[Category: L-fuconate dehydratase]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 08:35, 30 September 2014

OCA — Tue, 30 Sep 2014 08:35:05 GMT

←Older revision		Revision as of 08:35, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2hxu.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of K220A mutant of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and L-fuconate==
		+	<StructureSection load='2hxu' size='340' side='right' caption='[[2hxu]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2hxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris Xanthomonas campestris pv. campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HXU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LFC:6-DEOXY-L-GALACTONIC+ACID'>LFC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hne\|2hne]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hxu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hxu RCSB], [http://www.ebi.ac.uk/pdbsum/2hxu PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hx/2hxu_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many members of the mechanistically diverse enolase superfamily have unknown functions. In this report we use both genome (operon) context and screening of a library of acid sugars to assign the L-fuconate dehydratase (FucD) function to a member of the mandelate racemase (MR) subgroup of the superfamily encoded by the Xanthomonas campestris pv. campestris str. ATCC 33913 genome (GI:21233491). Orthologues of FucD are found in both bacteria and eukaryotes, the latter including the rTS beta protein in Homo sapiens that has been implicated in regulating thymidylate synthase activity. As suggested by sequence alignments and confirmed by high-resolution structures in the presence of active site ligands, FucD and MR share the same active site motif of functional groups: three carboxylate ligands for the essential Mg2+ located at the ends of the third, fourth, and fifth beta-strands in the (beta/alpha)7beta-barrel domain (Asp 248, Glu 274, and Glu 301, respectively), a Lys-x-Lys motif at the end of the second beta-strand (Lys 218 and Lys 220), a His-Asp dyad at the end of the seventh and beta-strands (His 351 and Asp 324, respectively), and a Glu at the end of the eighth beta-strand (Glu 382). The mechanism of the FucD reaction involves initial abstraction of the 2-proton by Lys 220, acid catalysis of the vinylogous beta-elimination of the 3-OH group by His 351, and stereospecific ketonization of the resulting enol, likely by the conjugate acid of Lys 220, to yield the 2-keto-3-deoxy-L-fuconate product. Screening of the library of acid sugars revealed substrate and functional promiscuity: In addition to L-fuconate, FucD also catalyzes the dehydration of L-galactonate, D-arabinonate, D-altronate, L-talonate, and D-ribonate. The dehydrations of L-fuconate, L-galactonate, and D-arabinonate are initiated by abstraction of the 2-protons by Lys 220. The dehydrations of L-talonate and D-ribonate are initiated by abstraction of the 2-protons by His 351; however, protonation of the enediolate intermediates by the conjugate acid of Lys 220 yields L-galactonate and D-arabinonate in competition with dehydration. The functional promiscuity discovered for FucD highlights possible structural mechanisms for evolution of function in the enolase superfamily.

-	~~{{STRUCTURE_2hxu\| PDB=2hxu \| SCENE= }}~~	+	Evolution of enzymatic activities in the enolase superfamily: L-fuconate dehydratase from Xanthomonas campestris.,Yew WS, Fedorov AA, Fedorov EV, Rakus JF, Pierce RW, Almo SC, Gerlt JA Biochemistry. 2006 Dec 12;45(49):14582-97. PMID:17144652<ref>PMID:17144652</ref>

-	~~===Crystal structure~~ of ~~K220A mutant of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and L-fuconate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17144652}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2hxu]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Xanthomonas_campestris_pv~~.~~_campestris Xanthomonas campestris pv. campestris]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017144652<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Xanthomonas campestris pv. campestris]]		[[Category: Xanthomonas campestris pv. campestris]]
	[[Category: Almo, S C.]]		[[Category: Almo, S C.]]

OCA: Protected "2hxu" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 13:34:19 GMT

Protected "2hxu" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:34, 7 January 2013

OCA at 13:34, 7 January 2013

OCA — Mon, 07 Jan 2013 13:34:16 GMT

←Older revision		Revision as of 13:34, 7 January 2013
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-	{{Seed}}
	[[Image:2hxu.png\|left\|200px]]		[[Image:2hxu.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2hxu", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2hxu\| PDB=2hxu \| SCENE= }}		{{STRUCTURE_2hxu\| PDB=2hxu \| SCENE= }}

	===Crystal structure of K220A mutant of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and L-fuconate===		===Crystal structure of K220A mutant of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and L-fuconate===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17144652}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17144652 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17144652}}		{{ABSTRACT_PUBMED_17144652}}

	==About this Structure==		==About this Structure==
-	~~2HXU~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris Xanthomonas campestris pv. campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA].	+	[[2hxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris Xanthomonas campestris pv. campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17144652~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017144652</ref><references group="xtra"/>
	[[Category: Xanthomonas campestris pv. campestris]]		[[Category: Xanthomonas campestris pv. campestris]]
	[[Category: Almo, S C.]]		[[Category: Almo, S C.]]
Line 34:		Line 22:
	[[Category: L-fuconate]]		[[Category: L-fuconate]]
	[[Category: L-fuconate dehydratase]]		[[Category: L-fuconate dehydratase]]
-		+	[[Category: Unknown function]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 00:30:14 2009''~~	+

OCA at 22:30, 17 February 2009

OCA — Tue, 17 Feb 2009 22:30:14 GMT

←Older revision		Revision as of 22:30, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2HXU is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris Xanthomonas campestris pv. campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA].	+	2HXU is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris Xanthomonas campestris pv. campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HXU OCA].

	==Reference==		==Reference==
-	Evolution of enzymatic activities in the enolase superfamily: L-fuconate dehydratase from Xanthomonas campestris., Yew WS, Fedorov AA, Fedorov EV, Rakus JF, Pierce RW, Almo SC, Gerlt JA, Biochemistry. 2006 Dec 12;45(49):14582-97. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17144652 17144652]~~	+	<ref group="xtra">PMID:17144652</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Xanthomonas campestris pv. campestris]]		[[Category: Xanthomonas campestris pv. campestris]]
	[[Category: Almo, S C.]]		[[Category: Almo, S C.]]
Line 36:		Line 35:
	[[Category: L-fuconate dehydratase]]		[[Category: L-fuconate dehydratase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 07~~:53:~~15 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:30:14 2009''