2hy7 - Revision history

OCA at 09:35, 14 February 2024

OCA — Wed, 14 Feb 2024 09:35:13 GMT

←Older revision		Revision as of 09:35, 14 February 2024
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	<StructureSection load='2hy7' size='340' side='right'caption='[[2hy7]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2hy7' size='340' side='right'caption='[[2hy7]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hy7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_campestris"_pammel_1895 "bacillus~~ campestris~~" pammel 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HY7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hy7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HY7 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>gumK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~339~~ "~~Bacillus campestris~~" ~~Pammel 1895])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'~~>[~~https://en.wikipedia.org/wiki/Glucuronosyltransferase Glucuronosyltransferase~~]~~, with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.~~1.~~17 2.4.1.17] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hy7 OCA], [https://pdbe.org/2hy7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hy7 RCSB], [https://www.ebi.ac.uk/pdbsum/2hy7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hy7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hy7 OCA], [https://pdbe.org/2hy7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hy7 RCSB], [https://www.ebi.ac.uk/pdbsum/2hy7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hy7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GUMK_XANCE GUMK_XANCE]] Catalyzes the transfer of a glucuronic acid (GlcA) residue from UDP-glucuronate to mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl to form the lipid-linked tetrasaccharide GlcA-Man-Glc(2)-PP-Pol, with a glucuronic acid-beta-mannose linkage. Is involved in the biosynthesis of the exopolysaccharide xanthan, since it catalyzes the fourth glycosylation step in the assembly of the pentasaccharide-P-P-polyisoprenyl repeating unit of xanthan. Is unable to use the trisaccharide acceptor freed from the pyrophosphate lipid moiety. Does not show specificity for the lipidic portion of the acceptor. Shows diminished activity when tested with 6-O-acetyl-mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl, a putative intermediate in the synthesis of xanthan; this could indicate that acetylation of the internal mannose takes place after the formation of the GumK product.<ref>PMID:14736729</ref>	+	[https://www.uniprot.org/uniprot/GUMK_XANCE GUMK_XANCE] Catalyzes the transfer of a glucuronic acid (GlcA) residue from UDP-glucuronate to mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl to form the lipid-linked tetrasaccharide GlcA-Man-Glc(2)-PP-Pol, with a glucuronic acid-beta-mannose linkage. Is involved in the biosynthesis of the exopolysaccharide xanthan, since it catalyzes the fourth glycosylation step in the assembly of the pentasaccharide-P-P-polyisoprenyl repeating unit of xanthan. Is unable to use the trisaccharide acceptor freed from the pyrophosphate lipid moiety. Does not show specificity for the lipidic portion of the acceptor. Shows diminished activity when tested with 6-O-acetyl-mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl, a putative intermediate in the synthesis of xanthan; this could indicate that acetylation of the internal mannose takes place after the formation of the GumK product.<ref>PMID:14736729</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hy7 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hy7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Xanthomonas campestris GumK (beta-1,2-glucuronosyltransferase) is a 44-kDa membrane-associated protein that is involved in the biosynthesis of xanthan, an exopolysaccharide crucial for this bacterium's phytopathogenicity. Xanthan also has many important industrial applications. The GumK enzyme is the founding member of the glycosyltransferase family 70 of carbohydrate-active enzymes, which is composed of bacterial glycosyltransferases involved in exopolysaccharide synthesis. No x-ray structures have been reported for this family. To better understand the mechanism of action of the bacterial glycosyltransferases in this family, the x-ray crystal structure of apo-GumK was solved at 1.9 angstroms resolution. The enzyme has two well defined Rossmann domains with a catalytic cleft between them, which is a typical feature of the glycosyltransferase B superfamily. Additionally, the crystal structure of GumK complexed with UDP was solved at 2.28 angstroms resolution. We identified a number of catalytically important residues, including Asp157, which serves as the general base in the transfer reaction. Residues Met231, Met273, Glu272, Tyr292, Met306, Lys307, and Gln310 interact with UDP, and mutation of these residues affected protein activity both in vitro and in vivo. The biological and structural data reported here shed light on the molecular basis for donor and acceptor selectivity in this glycosyltransferase family. These results also provide a rationale to obtain new polysaccharides by varying residues in the conserved alpha/beta/alpha structural motif of GumK.
-
-	Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase.,Barreras M, Salinas SR, Abdian PL, Kampel MA, Ielpi L J Biol Chem. 2008 Sep 5;283(36):25027-35. Epub 2008 Jul 2. PMID:18596046<ref>PMID:18596046</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2hy7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus campestris pammel 1895]]
-	[[Category: Glucuronosyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Barreras, M~~]]	+	[[Category: Xanthomonas campestris]]
-	[[Category: ~~Glycosyltransferase]]~~	+	[[Category: Barreras M]]
-	~~[[Category: Membrane-associated protein]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Xanthan]]~~	+
-	~~[[Category: Xanthomonas campestri~~]]	+

OCA at 07:24, 24 March 2021

OCA — Wed, 24 Mar 2021 07:24:19 GMT

OCA at 08:35, 4 July 2018

OCA — Wed, 04 Jul 2018 08:35:08 GMT

←Older revision		Revision as of 08:35, 4 July 2018
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		+
	==Crystal Structure of GumK, a beta-glucuronosyltransferase from Xanthomonas campestris==		==Crystal Structure of GumK, a beta-glucuronosyltransferase from Xanthomonas campestris==
	<StructureSection load='2hy7' size='340' side='right' caption='[[2hy7]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2hy7' size='340' side='right' caption='[[2hy7]], [[Resolution\|resolution]] 1.90Å' scene=''>
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	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gumK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=339 "Bacillus campestris" Pammel 1895])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gumK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=339 "Bacillus campestris" Pammel 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucuronosyltransferase Glucuronosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.17 2.4.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucuronosyltransferase Glucuronosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.17 2.4.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hy7 OCA], [http://pdbe.org/2hy7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hy7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hy7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hy7 OCA], [http://pdbe.org/2hy7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hy7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hy7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hy7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hy/2hy7_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hy/2hy7_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 07:02, 10 February 2016

OCA — Wed, 10 Feb 2016 07:02:50 GMT

←Older revision		Revision as of 07:02, 10 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hy7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:30, 11 September 2015

OCA — Fri, 11 Sep 2015 06:30:46 GMT

←Older revision		Revision as of 06:30, 11 September 2015
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	<StructureSection load='2hy7' size='340' side='right' caption='[[2hy7]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2hy7' size='340' side='right' caption='[[2hy7]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hy7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Xanthomonas_campestris Xanthomonas~~ campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HY7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hy7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_campestris"_pammel_1895 "bacillus campestris" pammel 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HY7 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gumK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=339 ~~Xanthomonas~~ campestris])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gumK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=339 "Bacillus campestris" Pammel 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucuronosyltransferase Glucuronosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.17 2.4.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucuronosyltransferase Glucuronosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.17 2.4.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hy7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hy7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hy7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hy7 OCA], [http://pdbe.org/2hy7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hy7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hy7 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q8GCH2_XANCP Q8GCH2_XANCP~~]] Catalyzes the transfer of a glucuronic acid (GlcA) residue from UDP-glucuronate to mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl to form the lipid-linked tetrasaccharide GlcA-Man-Glc(2)-PP-Pol, with a glucuronic acid-beta-mannose linkage. Is involved in the biosynthesis of the exopolysaccharide xanthan, since it catalyzes the fourth glycosylation step in the assembly of the pentasaccharide-P-P-polyisoprenyl repeating unit of xanthan. Is unable to use the trisaccharide acceptor freed from the pyrophosphate lipid moiety. Does not show specificity for the lipidic portion of the acceptor. Shows diminished activity when tested with 6-O-acetyl-mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl, a putative intermediate in the synthesis of xanthan; this could indicate that acetylation of the internal mannose takes place after the formation of the GumK product.<ref>PMID:14736729</ref>	+	[[http://www.uniprot.org/uniprot/GUMK_XANCE GUMK_XANCE]] Catalyzes the transfer of a glucuronic acid (GlcA) residue from UDP-glucuronate to mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl to form the lipid-linked tetrasaccharide GlcA-Man-Glc(2)-PP-Pol, with a glucuronic acid-beta-mannose linkage. Is involved in the biosynthesis of the exopolysaccharide xanthan, since it catalyzes the fourth glycosylation step in the assembly of the pentasaccharide-P-P-polyisoprenyl repeating unit of xanthan. Is unable to use the trisaccharide acceptor freed from the pyrophosphate lipid moiety. Does not show specificity for the lipidic portion of the acceptor. Shows diminished activity when tested with 6-O-acetyl-mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl, a putative intermediate in the synthesis of xanthan; this could indicate that acetylation of the internal mannose takes place after the formation of the GumK product.<ref>PMID:14736729</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2hy7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus campestris pammel 1895]]
	[[Category: Glucuronosyltransferase]]		[[Category: Glucuronosyltransferase]]
-	[[Category: Xanthomonas campestris]]
	[[Category: Barreras, M]]		[[Category: Barreras, M]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]

OCA at 12:36, 25 December 2014

OCA — Thu, 25 Dec 2014 12:36:28 GMT

←Older revision		Revision as of 12:36, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hy7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HY7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hy7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HY7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gumK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=339 Xanthomonas campestris])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gumK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=339 Xanthomonas campestris])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucuronosyltransferase Glucuronosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.17 2.4.1.17] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucuronosyltransferase Glucuronosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.17 2.4.1.17] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hy7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hy7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hy7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hy7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hy7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hy7 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q8GCH2_XANCP Q8GCH2_XANCP]] Catalyzes the transfer of a glucuronic acid (GlcA) residue from UDP-glucuronate to mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl to form the lipid-linked tetrasaccharide GlcA-Man-Glc(2)-PP-Pol, with a glucuronic acid-beta-mannose linkage. Is involved in the biosynthesis of the exopolysaccharide xanthan, since it catalyzes the fourth glycosylation step in the assembly of the pentasaccharide-P-P-polyisoprenyl repeating unit of xanthan. Is unable to use the trisaccharide acceptor freed from the pyrophosphate lipid moiety. Does not show specificity for the lipidic portion of the acceptor. Shows diminished activity when tested with 6-O-acetyl-mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl, a putative intermediate in the synthesis of xanthan; this could indicate that acetylation of the internal mannose takes place after the formation of the GumK product.<ref>PMID:14736729</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Glucuronosyltransferase]]		[[Category: Glucuronosyltransferase]]
	[[Category: Xanthomonas campestris]]		[[Category: Xanthomonas campestris]]
-	[[Category: Barreras, M~~.]]~~	+	[[Category: Barreras, M]]
-	~~[[Category: Glucuronosyltransferase~~]]	+
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
	[[Category: Membrane-associated protein]]		[[Category: Membrane-associated protein]]

OCA at 10:13, 30 September 2014

OCA — Tue, 30 Sep 2014 10:13:30 GMT

←Older revision		Revision as of 10:13, 30 September 2014
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-	[[~~Image~~:2hy7.~~png\|left~~\|200px]]	+	==Crystal Structure of GumK, a beta-glucuronosyltransferase from Xanthomonas campestris==
		+	<StructureSection load='2hy7' size='340' side='right' caption='[[2hy7]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2hy7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HY7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gumK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=339 Xanthomonas campestris])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucuronosyltransferase Glucuronosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.17 2.4.1.17] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hy7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hy7 RCSB], [http://www.ebi.ac.uk/pdbsum/2hy7 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hy/2hy7_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Xanthomonas campestris GumK (beta-1,2-glucuronosyltransferase) is a 44-kDa membrane-associated protein that is involved in the biosynthesis of xanthan, an exopolysaccharide crucial for this bacterium's phytopathogenicity. Xanthan also has many important industrial applications. The GumK enzyme is the founding member of the glycosyltransferase family 70 of carbohydrate-active enzymes, which is composed of bacterial glycosyltransferases involved in exopolysaccharide synthesis. No x-ray structures have been reported for this family. To better understand the mechanism of action of the bacterial glycosyltransferases in this family, the x-ray crystal structure of apo-GumK was solved at 1.9 angstroms resolution. The enzyme has two well defined Rossmann domains with a catalytic cleft between them, which is a typical feature of the glycosyltransferase B superfamily. Additionally, the crystal structure of GumK complexed with UDP was solved at 2.28 angstroms resolution. We identified a number of catalytically important residues, including Asp157, which serves as the general base in the transfer reaction. Residues Met231, Met273, Glu272, Tyr292, Met306, Lys307, and Gln310 interact with UDP, and mutation of these residues affected protein activity both in vitro and in vivo. The biological and structural data reported here shed light on the molecular basis for donor and acceptor selectivity in this glycosyltransferase family. These results also provide a rationale to obtain new polysaccharides by varying residues in the conserved alpha/beta/alpha structural motif of GumK.

-	~~{{STRUCTURE_2hy7\| PDB=2hy7 \| SCENE= }}~~	+	Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase.,Barreras M, Salinas SR, Abdian PL, Kampel MA, Ielpi L J Biol Chem. 2008 Sep 5;283(36):25027-35. Epub 2008 Jul 2. PMID:18596046<ref>PMID:18596046</ref>

-	~~===Crystal Structure of GumK~~, a ~~beta-glucuronosyltransferase from Xanthomonas campestris===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18596046}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2hy7]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Xanthomonas_campestris Xanthomonas campestris]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018596046<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Glucuronosyltransferase]]		[[Category: Glucuronosyltransferase]]
	[[Category: Xanthomonas campestris]]		[[Category: Xanthomonas campestris]]

OCA: Protected "2hy7" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 12:02:46 GMT

Protected "2hy7" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:02, 7 January 2013

OCA at 12:02, 7 January 2013

OCA — Mon, 07 Jan 2013 12:02:44 GMT

←Older revision		Revision as of 12:02, 7 January 2013
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-	{{Seed}}
	[[Image:2hy7.png\|left\|200px]]		[[Image:2hy7.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2hy7\| PDB=2hy7 \| SCENE= }}		{{STRUCTURE_2hy7\| PDB=2hy7 \| SCENE= }}

	===Crystal Structure of GumK, a beta-glucuronosyltransferase from Xanthomonas campestris===		===Crystal Structure of GumK, a beta-glucuronosyltransferase from Xanthomonas campestris===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18596046}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18596046 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_18596046}}		{{ABSTRACT_PUBMED_18596046}}

	==About this Structure==		==About this Structure==
-	~~2HY7~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA].	+	[[2hy7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18596046~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018596046</ref><references group="xtra"/>
	[[Category: Glucuronosyltransferase]]		[[Category: Glucuronosyltransferase]]
	[[Category: Xanthomonas campestris]]		[[Category: Xanthomonas campestris]]
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	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
	[[Category: Membrane-associated protein]]		[[Category: Membrane-associated protein]]
		+	[[Category: Transferase]]
	[[Category: Xanthan]]		[[Category: Xanthan]]
	[[Category: Xanthomonas campestri]]		[[Category: Xanthomonas campestri]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:20:55 2009''

OCA at 14:20, 17 February 2009

OCA — Tue, 17 Feb 2009 14:20:56 GMT

←Older revision		Revision as of 14:20, 17 February 2009
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	==About this Structure==		==About this Structure==
-	2HY7 is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA].	+	2HY7 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA].

	==Reference==		==Reference==
-	~~Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase., Barreras M, Salinas SR, Abdian PL, Kampel MA, Ielpi L, J Biol Chem. 2008 Sep 5;283(36):25027-35. Epub 2008 Jul 2.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18596046 18596046]~~	+	<ref group="xtra">PMID:18596046</ref><references group="xtra"/>
	[[Category: Glucuronosyltransferase]]		[[Category: Glucuronosyltransferase]]
-	[[Category: Single protein]]
	[[Category: Xanthomonas campestris]]		[[Category: Xanthomonas campestris]]
	[[Category: Barreras, M.]]		[[Category: Barreras, M.]]
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	[[Category: Xanthomonas campestri]]		[[Category: Xanthomonas campestri]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Oct 1 21~~:21:~~04 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:20:55 2009''

←Older revision		Revision as of 07:24, 24 March 2021
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	==Crystal Structure of GumK, a beta-glucuronosyltransferase from Xanthomonas campestris==		==Crystal Structure of GumK, a beta-glucuronosyltransferase from Xanthomonas campestris==
-	<StructureSection load='2hy7' size='340' side='right' caption='[[2hy7]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='2hy7' size='340' side='right'caption='[[2hy7]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hy7]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_campestris"_pammel_1895 "bacillus campestris" pammel 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HY7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hy7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_campestris"_pammel_1895 "bacillus campestris" pammel 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HY7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HY7 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gumK ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=339 "Bacillus campestris" Pammel 1895])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gumK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=339 "Bacillus campestris" Pammel 1895])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glucuronosyltransferase Glucuronosyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.17 2.4.1.17] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glucuronosyltransferase Glucuronosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.17 2.4.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2hy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hy7 OCA], [~~http~~://pdbe.org/2hy7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2hy7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2hy7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2hy7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hy7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hy7 OCA], [https://pdbe.org/2hy7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hy7 RCSB], [https://www.ebi.ac.uk/pdbsum/2hy7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hy7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GUMK_XANCE GUMK_XANCE]] Catalyzes the transfer of a glucuronic acid (GlcA) residue from UDP-glucuronate to mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl to form the lipid-linked tetrasaccharide GlcA-Man-Glc(2)-PP-Pol, with a glucuronic acid-beta-mannose linkage. Is involved in the biosynthesis of the exopolysaccharide xanthan, since it catalyzes the fourth glycosylation step in the assembly of the pentasaccharide-P-P-polyisoprenyl repeating unit of xanthan. Is unable to use the trisaccharide acceptor freed from the pyrophosphate lipid moiety. Does not show specificity for the lipidic portion of the acceptor. Shows diminished activity when tested with 6-O-acetyl-mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl, a putative intermediate in the synthesis of xanthan; this could indicate that acetylation of the internal mannose takes place after the formation of the GumK product.<ref>PMID:14736729</ref>	+	[[https://www.uniprot.org/uniprot/GUMK_XANCE GUMK_XANCE]] Catalyzes the transfer of a glucuronic acid (GlcA) residue from UDP-glucuronate to mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl to form the lipid-linked tetrasaccharide GlcA-Man-Glc(2)-PP-Pol, with a glucuronic acid-beta-mannose linkage. Is involved in the biosynthesis of the exopolysaccharide xanthan, since it catalyzes the fourth glycosylation step in the assembly of the pentasaccharide-P-P-polyisoprenyl repeating unit of xanthan. Is unable to use the trisaccharide acceptor freed from the pyrophosphate lipid moiety. Does not show specificity for the lipidic portion of the acceptor. Shows diminished activity when tested with 6-O-acetyl-mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl, a putative intermediate in the synthesis of xanthan; this could indicate that acetylation of the internal mannose takes place after the formation of the GumK product.<ref>PMID:14736729</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Bacillus campestris pammel 1895]]		[[Category: Bacillus campestris pammel 1895]]
	[[Category: Glucuronosyltransferase]]		[[Category: Glucuronosyltransferase]]
		+	[[Category: Large Structures]]
	[[Category: Barreras, M]]		[[Category: Barreras, M]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]