2hz6 - Revision history

OCA at 09:00, 21 February 2024

OCA — Wed, 21 Feb 2024 09:00:40 GMT

←Older revision		Revision as of 09:00, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='2hz6' size='340' side='right'caption='[[2hz6]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='2hz6' size='340' side='right'caption='[[2hz6]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [https://pdbe.org/2hz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [https://www.ebi.ac.uk/pdbsum/2hz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hz6 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [https://pdbe.org/2hz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [https://www.ebi.ac.uk/pdbsum/2hz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hz6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ERN1_HUMAN ERN1_HUMAN]] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.<ref>PMID:9637683</ref> <ref>PMID:11175748</ref> <ref>PMID:12637535</ref> [UniProtKB:Q9EQY0]	+	[https://www.uniprot.org/uniprot/ERN1_HUMAN ERN1_HUMAN] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.<ref>PMID:9637683</ref> <ref>PMID:11175748</ref> <ref>PMID:12637535</ref> [UniProtKB:Q9EQY0]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hz6 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hz6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1) and PKR-related ER kinase (PERK) are two type I transmembrane ER-localized protein kinase receptors that signal the UPR through a process that involves homodimerization and autophosphorylation. To elucidate the molecular basis of the ER transmembrane signaling event, we determined the x-ray crystal structure of the luminal domain of human IRE1alpha. The monomer of the luminal domain comprises a unique fold of a triangular assembly of beta-sheet clusters. Structural analysis identified an extensive dimerization interface stabilized by hydrogen bonds and hydrophobic interactions. Dimerization creates an MHC-like groove at the interface. However, because this groove is too narrow for peptide binding and the purified luminal domain forms high-affinity dimers in vitro, peptide binding to this groove is not required for dimerization. Consistent with our structural observations, mutations that disrupt the dimerization interface produced IRE1alpha molecules that failed to either dimerize or activate the UPR upon ER stress. In addition, mutations in a structurally homologous region within PERK also prevented dimerization. Our structural, biochemical, and functional studies in vivo altogether demonstrate that IRE1 and PERK have conserved a common molecular interface necessary and sufficient for dimerization and UPR signaling.
-
-	The crystal structure of human IRE1 luminal domain reveals a conserved dimerization interface required for activation of the unfolded protein response.,Zhou J, Liu CY, Back SH, Clark RL, Peisach D, Xu Z, Kaufman RJ Proc Natl Acad Sci U S A. 2006 Sep 26;103(39):14343-8. Epub 2006 Sep 14. PMID:16973740<ref>PMID:16973740</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2hz6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kaufman~~, R J~~]]	+	[[Category: Kaufman RJ]]
-	[[Category: Xu, Z]]	+	[[Category: Xu Z]]
-	[[Category: Zhou, J]]	+	[[Category: Zhou J]]
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Triangular beta-sheet cluster~~]]	+

OCA at 17:48, 20 October 2021

OCA — Wed, 20 Oct 2021 17:48:23 GMT

←Older revision		Revision as of 17:48, 20 October 2021
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	<StructureSection load='2hz6' size='340' side='right'caption='[[2hz6]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='2hz6' size='340' side='right'caption='[[2hz6]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [~~http~~://pdbe.org/2hz6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2hz6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2hz6 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [https://pdbe.org/2hz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [https://www.ebi.ac.uk/pdbsum/2hz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hz6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ERN1_HUMAN ERN1_HUMAN]] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.<ref>PMID:9637683</ref> <ref>PMID:11175748</ref> <ref>PMID:12637535</ref> [UniProtKB:Q9EQY0]	+	[[https://www.uniprot.org/uniprot/ERN1_HUMAN ERN1_HUMAN]] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.<ref>PMID:9637683</ref> <ref>PMID:11175748</ref> <ref>PMID:12637535</ref> [UniProtKB:Q9EQY0]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:40, 8 April 2020

OCA — Wed, 08 Apr 2020 07:40:35 GMT

←Older revision		Revision as of 07:40, 8 April 2020
Line 1:		Line 1:

	==The crystal structure of human IRE1-alpha luminal domain==		==The crystal structure of human IRE1-alpha luminal domain==
-	<StructureSection load='2hz6' size='340' side='right' caption='[[2hz6]], [[Resolution\|resolution]] 3.10Å' scene=''>	+	<StructureSection load='2hz6' size='340' side='right'caption='[[2hz6]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [http://pdbe.org/2hz6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [http://www.ebi.ac.uk/pdbsum/2hz6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hz6 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [http://pdbe.org/2hz6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [http://www.ebi.ac.uk/pdbsum/2hz6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hz6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Kaufman, R J]]		[[Category: Kaufman, R J]]
	[[Category: Xu, Z]]		[[Category: Xu, Z]]

OCA at 08:22, 4 July 2018

OCA — Wed, 04 Jul 2018 08:22:00 GMT

←Older revision		Revision as of 08:22, 4 July 2018
Line 1:		Line 1:
		+
	==The crystal structure of human IRE1-alpha luminal domain==		==The crystal structure of human IRE1-alpha luminal domain==
	<StructureSection load='2hz6' size='340' side='right' caption='[[2hz6]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='2hz6' size='340' side='right' caption='[[2hz6]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [http://pdbe.org/2hz6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [http://www.ebi.ac.uk/pdbsum/2hz6 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [http://pdbe.org/2hz6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [http://www.ebi.ac.uk/pdbsum/2hz6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hz6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hz/2hz6_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hz/2hz6_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hz6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:49, 11 September 2015

OCA — Fri, 11 Sep 2015 10:49:53 GMT

←Older revision		Revision as of 10:49, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2hz6' size='340' side='right' caption='[[2hz6]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='2hz6' size='340' side='right' caption='[[2hz6]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [http://www.ebi.ac.uk/pdbsum/2hz6 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [http://pdbe.org/2hz6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [http://www.ebi.ac.uk/pdbsum/2hz6 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ERN1_HUMAN ERN1_HUMAN]] Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.<ref>PMID:9637683</ref> <ref>PMID:11175748</ref> <ref>PMID:12637535</ref> [UniProtKB:Q9EQY0]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 23:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2hz6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 30:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Kaufman, R J]]		[[Category: Kaufman, R J]]
	[[Category: Xu, Z]]		[[Category: Xu, Z]]

OCA at 08:26, 16 January 2015

OCA — Fri, 16 Jan 2015 08:26:13 GMT

←Older revision		Revision as of 08:26, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [http://www.ebi.ac.uk/pdbsum/2hz6 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [http://www.ebi.ac.uk/pdbsum/2hz6 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Kaufman, R J.]]	+	[[Category: Kaufman, R J]]
-	[[Category: Xu, Z.]]	+	[[Category: Xu, Z]]
-	[[Category: Zhou, J.]]	+	[[Category: Zhou, J]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]
	[[Category: Triangular beta-sheet cluster]]		[[Category: Triangular beta-sheet cluster]]

OCA at 02:29, 29 September 2014

OCA — Mon, 29 Sep 2014 02:29:20 GMT

←Older revision		Revision as of 02:29, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2hz6.~~png\|left~~\|200px]]	+	==The crystal structure of human IRE1-alpha luminal domain==
		+	<StructureSection load='2hz6' size='340' side='right' caption='[[2hz6]], [[Resolution\|resolution]] 3.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2hz6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HZ6 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hz6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2hz6 RCSB], [http://www.ebi.ac.uk/pdbsum/2hz6 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hz/2hz6_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1) and PKR-related ER kinase (PERK) are two type I transmembrane ER-localized protein kinase receptors that signal the UPR through a process that involves homodimerization and autophosphorylation. To elucidate the molecular basis of the ER transmembrane signaling event, we determined the x-ray crystal structure of the luminal domain of human IRE1alpha. The monomer of the luminal domain comprises a unique fold of a triangular assembly of beta-sheet clusters. Structural analysis identified an extensive dimerization interface stabilized by hydrogen bonds and hydrophobic interactions. Dimerization creates an MHC-like groove at the interface. However, because this groove is too narrow for peptide binding and the purified luminal domain forms high-affinity dimers in vitro, peptide binding to this groove is not required for dimerization. Consistent with our structural observations, mutations that disrupt the dimerization interface produced IRE1alpha molecules that failed to either dimerize or activate the UPR upon ER stress. In addition, mutations in a structurally homologous region within PERK also prevented dimerization. Our structural, biochemical, and functional studies in vivo altogether demonstrate that IRE1 and PERK have conserved a common molecular interface necessary and sufficient for dimerization and UPR signaling.

-	~~{{STRUCTURE_2hz6\| PDB=2hz6 \| SCENE= }}~~	+	The crystal structure of human IRE1 luminal domain reveals a conserved dimerization interface required for activation of the unfolded protein response.,Zhou J, Liu CY, Back SH, Clark RL, Peisach D, Xu Z, Kaufman RJ Proc Natl Acad Sci U S A. 2006 Sep 26;103(39):14343-8. Epub 2006 Sep 14. PMID:16973740<ref>PMID:16973740</ref>

-	~~===The crystal structure~~ of ~~human IRE1-alpha luminal domain===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16973740}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2hz6]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA].~~	+

	==See Also==		==See Also==
	*[[Ire1\|Ire1]]		*[[Ire1\|Ire1]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016973740<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Kaufman, R J.]]		[[Category: Kaufman, R J.]]

OCA: Protected "2hz6" [edit=sysop:move=sysop]

OCA — Sat, 20 Oct 2012 18:19:01 GMT

Protected "2hz6" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:19, 20 October 2012

OCA at 18:18, 20 October 2012

OCA — Sat, 20 Oct 2012 18:18:59 GMT

←Older revision		Revision as of 18:18, 20 October 2012
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-	{{Seed}}
	[[Image:2hz6.png\|left\|200px]]		[[Image:2hz6.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2hz6\| PDB=2hz6 \| SCENE= }}		{{STRUCTURE_2hz6\| PDB=2hz6 \| SCENE= }}

	===The crystal structure of human IRE1-alpha luminal domain===		===The crystal structure of human IRE1-alpha luminal domain===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16973740}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16973740 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_16973740}}		{{ABSTRACT_PUBMED_16973740}}

	==About this Structure==		==About this Structure==
-	~~2HZ6~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA].	+	[[2hz6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA].
		+
		+	==See Also==
		+	*[[Ire1\|Ire1]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16973740~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016973740</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Kaufman, R J.]]		[[Category: Kaufman, R J.]]
	[[Category: Xu, Z.]]		[[Category: Xu, Z.]]
	[[Category: Zhou, J.]]		[[Category: Zhou, J.]]
		+	[[Category: Signaling protein]]
	[[Category: Triangular beta-sheet cluster]]		[[Category: Triangular beta-sheet cluster]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:18:29 2009''

OCA at 18:18, 17 February 2009

OCA — Tue, 17 Feb 2009 18:18:30 GMT

←Older revision		Revision as of 18:18, 17 February 2009
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	==About this Structure==		==About this Structure==
-	2HZ6 is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA].	+	2HZ6 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZ6 OCA].

	==Reference==		==Reference==
-	The crystal structure of human IRE1 luminal domain reveals a conserved dimerization interface required for activation of the unfolded protein response., Zhou J, Liu CY, Back SH, Clark RL, Peisach D, Xu Z, Kaufman RJ, Proc Natl Acad Sci U S A. 2006 Sep 26;103(39):14343-8. Epub 2006 Sep 14. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16973740 16973740]~~	+	<ref group="xtra">PMID:16973740</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Single protein]]
	[[Category: Kaufman, R J.]]		[[Category: Kaufman, R J.]]
	[[Category: Xu, Z.]]		[[Category: Xu, Z.]]
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	[[Category: Triangular beta-sheet cluster]]		[[Category: Triangular beta-sheet cluster]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 04~~:11:~~01 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:18:29 2009''