2i18 - Revision history

OCA at 06:41, 1 May 2024

OCA — Wed, 01 May 2024 06:41:12 GMT

←Older revision		Revision as of 06:41, 1 May 2024
Line 1:		Line 1:

	==The refined structure of C-terminal domain of an EF-hand Calcium binding Protein from Entamoeba Histolytica==		==The refined structure of C-terminal domain of an EF-hand Calcium binding Protein from Entamoeba Histolytica==
-	<StructureSection load='2i18' size='340' side='right'caption='[[2i18~~]], [[NMR_Ensembles_of_Models \| 16 NMR models~~]]' scene=''>	+	<StructureSection load='2i18' size='340' side='right'caption='[[2i18]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2i18]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ev7 2ev7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I18 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I18 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2i18]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ev7 2ev7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I18 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I18 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1jfk\|1jfk]], [[1jfj\|1jfj]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i18 OCA], [https://pdbe.org/2i18 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i18 RCSB], [https://www.ebi.ac.uk/pdbsum/2i18 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i18 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i18 OCA], [https://pdbe.org/2i18 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i18 RCSB], [https://www.ebi.ac.uk/pdbsum/2i18 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i18 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CALBP_ENTHI CALBP_ENTHI]] Could play a role in the transduction of secondary messages on binding of calcium.	+	[https://www.uniprot.org/uniprot/CALBP_ENTHI CALBP_ENTHI] Could play a role in the transduction of secondary messages on binding of calcium.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i18 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i18 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Lanthanide ions (Ln(3+)), which have ionic radii similar to those of Ca(2+), can displace the latter in a calcium binding protein, without affecting its tertiary structure. The paramagnetic Ln(3+) possesses large anisotropic magnetic susceptibilities and produce pseudocontact shifts (PCSs), which have r(-3) dependence. The PCS can be seen for spins as far as 45 A from the paramagnetic ion. They aid in structure refinement of proteins by providing long-range distance constraints. Besides, they can be used to determine the interdomain orientation in multidomain proteins. This is particularly important in the context of a calcium binding protein from Entamoeba histolytica (EhCaBP), which consists of two globular domains connected by a flexible linker region containing 8 residues. As a first step to obtain the interdomain orientation in EhCaBP, a suite of 2D and 3D heteronuclear experiments were recorded on EhCaBP by displacing calcium with Ce(3+), Ho(3+), Er(3+), Tm(3+), Dy(3+), and Yb(3+) ions in separate experiments, and the PCS of (1)H(N) and (15)N spins were measured. Such data have been used in the refinement of the individual domain structures of the protein in parallel with the calculation of the respective magnetic anisotropy tensorial values, which differ substantially (2.1-2.8 times) from what is found in other Ca(2+) binding loops. This study provides a structural basis for such variations in the magnetic anisotropy tensorial values.
-
-	Structural basis for the observed differential magnetic anisotropic tensorial values in calcium binding proteins.,Mustafi SM, Mukherjee S, Chary KV, Cavallaro G Proteins. 2006 Nov 15;65(3):656-69. PMID:16981203<ref>PMID:16981203</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2i18" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Entamoeba histolytica]]		[[Category: Entamoeba histolytica]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chary~~, K V.R~~]]	+	[[Category: Chary KVR]]
-	[[Category: Mukherjee, S]]	+	[[Category: Mukherjee S]]
-	[[Category: Mustafi~~, S M]]~~	+	[[Category: Mustafi SM]]
-	~~[[Category: Alpha helix]]~~	+
-	~~[[Category: Beta sheet]]~~	+
-	~~[[Category: Calcium binding loop]]~~	+
-	~~[[Category: Metal binding protein~~]]	+

OCA at 20:42, 20 October 2021

OCA — Wed, 20 Oct 2021 20:42:21 GMT

←Older revision		Revision as of 20:42, 20 October 2021
Line 3:		Line 3:
	<StructureSection load='2i18' size='340' side='right'caption='[[2i18]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='2i18' size='340' side='right'caption='[[2i18]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i18]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ev7 2ev7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I18 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2I18 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i18]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ev7 2ev7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I18 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I18 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jfk\|1jfk]], [[1jfj\|1jfj]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jfk\|1jfk]], [[1jfj\|1jfj]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2i18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i18 OCA], [~~http~~://pdbe.org/2i18 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2i18 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2i18 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2i18 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i18 OCA], [https://pdbe.org/2i18 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i18 RCSB], [https://www.ebi.ac.uk/pdbsum/2i18 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i18 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CALBP_ENTHI CALBP_ENTHI]] Could play a role in the transduction of secondary messages on binding of calcium.	+	[[https://www.uniprot.org/uniprot/CALBP_ENTHI CALBP_ENTHI]] Could play a role in the transduction of secondary messages on binding of calcium.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 19:35, 11 December 2019

OCA — Wed, 11 Dec 2019 19:35:59 GMT

←Older revision		Revision as of 19:35, 11 December 2019
Line 1:		Line 1:

	==The refined structure of C-terminal domain of an EF-hand Calcium binding Protein from Entamoeba Histolytica==		==The refined structure of C-terminal domain of an EF-hand Calcium binding Protein from Entamoeba Histolytica==
-	<StructureSection load='2i18' size='340' side='right' caption='[[2i18]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>	+	<StructureSection load='2i18' size='340' side='right'caption='[[2i18]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2i18]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ev7 2ev7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I18 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2i18]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ev7 2ev7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I18 FirstGlance]. <br>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Entamoeba histolytica]]		[[Category: Entamoeba histolytica]]
		+	[[Category: Large Structures]]
	[[Category: Chary, K V.R]]		[[Category: Chary, K V.R]]
	[[Category: Mukherjee, S]]		[[Category: Mukherjee, S]]

OCA at 06:38, 31 January 2018

OCA — Wed, 31 Jan 2018 06:38:49 GMT

←Older revision		Revision as of 06:38, 31 January 2018
Line 1:		Line 1:
		+
	==The refined structure of C-terminal domain of an EF-hand Calcium binding Protein from Entamoeba Histolytica==		==The refined structure of C-terminal domain of an EF-hand Calcium binding Protein from Entamoeba Histolytica==
	<StructureSection load='2i18' size='340' side='right' caption='[[2i18]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='2i18' size='340' side='right' caption='[[2i18]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jfk\|1jfk]], [[1jfj\|1jfj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jfk\|1jfk]], [[1jfj\|1jfj]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i18 OCA], [http://pdbe.org/2i18 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i18 RCSB], [http://www.ebi.ac.uk/pdbsum/2i18 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i18 OCA], [http://pdbe.org/2i18 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i18 RCSB], [http://www.ebi.ac.uk/pdbsum/2i18 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i18 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i1/2i18_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i1/2i18_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:15, 8 February 2016

OCA — Mon, 08 Feb 2016 08:15:40 GMT

←Older revision		Revision as of 08:15, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i18 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:37, 10 September 2015

OCA — Thu, 10 Sep 2015 09:37:23 GMT

←Older revision		Revision as of 09:37, 10 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jfk\|1jfk]], [[1jfj\|1jfj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jfk\|1jfk]], [[1jfj\|1jfj]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i18 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i18 RCSB], [http://www.ebi.ac.uk/pdbsum/2i18 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i18 OCA], [http://pdbe.org/2i18 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i18 RCSB], [http://www.ebi.ac.uk/pdbsum/2i18 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2i18" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:54, 24 December 2014

OCA — Wed, 24 Dec 2014 21:54:28 GMT

←Older revision		Revision as of 21:54, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2i18]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ev7 2ev7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I18 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2i18]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ev7 2ev7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I18 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jfk\|1jfk]], [[1jfj\|1jfj]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jfk\|1jfk]], [[1jfj\|1jfj]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i18 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i18 RCSB], [http://www.ebi.ac.uk/pdbsum/2i18 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i18 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i18 RCSB], [http://www.ebi.ac.uk/pdbsum/2i18 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CALBP_ENTHI CALBP_ENTHI]] Could play a role in the transduction of secondary messages on binding of calcium.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	[[Category: Entamoeba histolytica]]		[[Category: Entamoeba histolytica]]
	[[Category: Chary, K V.R]]		[[Category: Chary, K V.R]]
-	[[Category: Mukherjee, S.]]	+	[[Category: Mukherjee, S]]
-	[[Category: Mustafi, S M.]]	+	[[Category: Mustafi, S M]]
	[[Category: Alpha helix]]		[[Category: Alpha helix]]
	[[Category: Beta sheet]]		[[Category: Beta sheet]]
	[[Category: Calcium binding loop]]		[[Category: Calcium binding loop]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]

OCA at 10:47, 30 September 2014

OCA — Tue, 30 Sep 2014 10:47:38 GMT

←Older revision		Revision as of 10:47, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2i18.~~png~~\|~~left~~\|200px]]	+	==The refined structure of C-terminal domain of an EF-hand Calcium binding Protein from Entamoeba Histolytica==
		+	<StructureSection load='2i18' size='340' side='right' caption='[[2i18]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2i18]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ev7 2ev7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I18 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LA:LANTHANUM+(III)+ION'>LA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jfk\|1jfk]], [[1jfj\|1jfj]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i18 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i18 RCSB], [http://www.ebi.ac.uk/pdbsum/2i18 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i1/2i18_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lanthanide ions (Ln(3+)), which have ionic radii similar to those of Ca(2+), can displace the latter in a calcium binding protein, without affecting its tertiary structure. The paramagnetic Ln(3+) possesses large anisotropic magnetic susceptibilities and produce pseudocontact shifts (PCSs), which have r(-3) dependence. The PCS can be seen for spins as far as 45 A from the paramagnetic ion. They aid in structure refinement of proteins by providing long-range distance constraints. Besides, they can be used to determine the interdomain orientation in multidomain proteins. This is particularly important in the context of a calcium binding protein from Entamoeba histolytica (EhCaBP), which consists of two globular domains connected by a flexible linker region containing 8 residues. As a first step to obtain the interdomain orientation in EhCaBP, a suite of 2D and 3D heteronuclear experiments were recorded on EhCaBP by displacing calcium with Ce(3+), Ho(3+), Er(3+), Tm(3+), Dy(3+), and Yb(3+) ions in separate experiments, and the PCS of (1)H(N) and (15)N spins were measured. Such data have been used in the refinement of the individual domain structures of the protein in parallel with the calculation of the respective magnetic anisotropy tensorial values, which differ substantially (2.1-2.8 times) from what is found in other Ca(2+) binding loops. This study provides a structural basis for such variations in the magnetic anisotropy tensorial values.

-	~~{{STRUCTURE_2i18\| PDB=2i18 \| SCENE= }}~~	+	Structural basis for the observed differential magnetic anisotropic tensorial values in calcium binding proteins.,Mustafi SM, Mukherjee S, Chary KV, Cavallaro G Proteins. 2006 Nov 15;65(3):656-69. PMID:16981203<ref>PMID:16981203</ref>

-	~~===The refined structure~~ of ~~C-terminal domain of an EF-hand Calcium binding Protein from Entamoeba Histolytica===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16981203}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2i18]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. This structure supersedes~~ the ~~now removed PDB entry [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/send-pdb?obs=1&id=2ev7 2ev7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I18 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016981203<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Entamoeba histolytica]]		[[Category: Entamoeba histolytica]]
	[[Category: Chary, K V.R]]		[[Category: Chary, K V.R]]

OCA: Protected "2i18" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 15:16:50 GMT

Protected "2i18" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:16, 7 January 2013

OCA at 15:16, 7 January 2013

OCA — Mon, 07 Jan 2013 15:16:48 GMT

←Older revision		Revision as of 15:16, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2i18.png\|left\|200px]]		[[Image:2i18.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2i18", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2i18\| PDB=2i18 \| SCENE= }}		{{STRUCTURE_2i18\| PDB=2i18 \| SCENE= }}

	===The refined structure of C-terminal domain of an EF-hand Calcium binding Protein from Entamoeba Histolytica===		===The refined structure of C-terminal domain of an EF-hand Calcium binding Protein from Entamoeba Histolytica===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16981203}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16981203 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16981203}}		{{ABSTRACT_PUBMED_16981203}}

	==About this Structure==		==About this Structure==
-	~~2I18~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ev7 2ev7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I18 OCA].	+	[[2i18]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ev7 2ev7]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I18 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16981203~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016981203</ref><references group="xtra"/>
	[[Category: Entamoeba histolytica]]		[[Category: Entamoeba histolytica]]
	[[Category: Chary, K V.R]]		[[Category: Chary, K V.R]]
Line 31:		Line 19:
	[[Category: Beta sheet]]		[[Category: Beta sheet]]
	[[Category: Calcium binding loop]]		[[Category: Calcium binding loop]]
-		+	[[Category: Metal binding protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 20:29:40 2009''~~	+