2i1t - Revision history

OCA at 16:37, 13 December 2023

OCA — Wed, 13 Dec 2023 16:37:59 GMT

←Older revision		Revision as of 16:37, 13 December 2023
Line 1:		Line 1:

	==Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels==		==Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels==
-	<StructureSection load='2i1t' size='340' side='right'caption='[[2i1t~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2i1t' size='340' side='right'caption='[[2i1t]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i1t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chilobrachys_jingzhao~~ Chilobrachys ~~jingzhao~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I1T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i1t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chilobrachys_guangxiensis Chilobrachys guangxiensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I1T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [https://pdbe.org/2i1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [https://www.ebi.ac.uk/pdbsum/2i1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i1t ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [https://pdbe.org/2i1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [https://www.ebi.ac.uk/pdbsum/2i1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i1t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/JZTX3_CHIGU JZTX3_CHIGU]] Selectively inhibits activation of voltage-gated sodium channels (VGSC) (Nav1.5/SCN5A) in rat cardiac myocytes, followed by shifting activated voltage in a depolarizing direction. The binding site on VGSC is suggested to be site 4 located at the extracellular S3-S4 loop of the channel. Also binds to voltage-gated potassium channels (Kv2.1/KCNB1).<ref>PMID:17150181</ref>	+	[https://www.uniprot.org/uniprot/JZTX3_CHIGU JZTX3_CHIGU] Selectively inhibits activation of voltage-gated sodium channels (VGSC) (Nav1.5/SCN5A) in rat cardiac myocytes, followed by shifting activated voltage in a depolarizing direction. The binding site on VGSC is suggested to be site 4 located at the extracellular S3-S4 loop of the channel. Also binds to voltage-gated potassium channels (Kv2.1/KCNB1).<ref>PMID:17150181</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	We have isolated a cardiotoxin, denoted jingzhaotoxin-III (JZTX-III), from the venom of the Chinese spider Chilobrachys jingzhao. The toxin contains 36 residues stabilized by three intracellular disulfide bridges (I-IV, II-V, and III-VI), assigned by a chemical strategy of partial reduction and sequence analysis. Cloned and sequenced using 3'-rapid amplification of cDNA ends and 5'-rapid amplification of cDNA ends, the full-length cDNA encoded a 63-residue precursor of JZTX-III. Different from other spider peptides, it contains an uncommon endoproteolytic site (-X-Ser-) anterior to mature protein and the intervening regions of 5 residues, which is the smallest in spider toxin cDNAs identified to date. Under whole cell recording, JZTX-III showed no effects on voltage-gated sodium channels (VGSCs) or calcium channels in dorsal root ganglion neurons, whereas it significantly inhibited tetrodotoxin-resistant VGSCs with an IC(50) value of 0.38 microm in rat cardiac myocytes. Different from scorpion beta-toxins, it caused a 10-mV depolarizing shift in the channel activation threshold. The binding site for JZTX-III on VGSCs is further suggested to be site 4 with a simple competitive assay, which at 10 microm eliminated the slowing currents induced by Buthus martensi Karsch I (BMK-I, scorpion alpha-like toxin) completely. JZTX-III shows higher selectivity for VGSC isoforms than other spider toxins affecting VGSCs, and the toxin hopefully represents an important ligand for discriminating cardiac VGSC subtype.	+
-		+
-	Jingzhaotoxin-III, a novel spider toxin inhibiting activation of voltage-gated sodium channel in rat cardiac myocytes.,Xiao Y, Tang J, Yang Y, Wang M, Hu W, Xie J, Zeng X, Liang S J Biol Chem. 2004 Jun 18;279(25):26220-6. Epub 2004 Apr 14. PMID:15084603<ref>PMID:15084603</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 2i1t" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chilobrachys ~~jingzhao~~]]	+	[[Category: Chilobrachys guangxiensis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Liang, S]]	+	[[Category: Liang S]]
-	[[Category: Liao, Z]]	+	[[Category: Liao Z]]
-	[[Category: Peng, K]]	+	[[Category: Peng K]]
-	~~[[Category: Cardiac myocyte]]~~	+
-	~~[[Category: Jingzhaotoxin-iii]]~~	+
-	~~[[Category: Kv2 1 channel]]~~	+
-	~~[[Category: Nav channel]]~~	+
-	~~[[Category: Solution structure]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 10:24, 12 January 2022

OCA — Wed, 12 Jan 2022 10:24:20 GMT

←Older revision		Revision as of 10:24, 12 January 2022
Line 3:		Line 3:
	<StructureSection load='2i1t' size='340' side='right'caption='[[2i1t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2i1t' size='340' side='right'caption='[[2i1t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i1t]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chilobrachys_jingzhao Chilobrachys jingzhao]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2I1T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i1t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chilobrachys_jingzhao Chilobrachys jingzhao]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I1T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [~~http~~://pdbe.org/2i1t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2i1t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2i1t ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [https://pdbe.org/2i1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [https://www.ebi.ac.uk/pdbsum/2i1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i1t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/JZTX3_CHIGU JZTX3_CHIGU]] Selectively inhibits activation of voltage-gated sodium channels (VGSC) (Nav1.5/SCN5A) in rat cardiac myocytes, followed by shifting activated voltage in a depolarizing direction. The binding site on VGSC is suggested to be site 4 located at the extracellular S3-S4 loop of the channel. Also binds to voltage-gated potassium channels (Kv2.1/KCNB1).<ref>PMID:17150181</ref>	+	[[https://www.uniprot.org/uniprot/JZTX3_CHIGU JZTX3_CHIGU]] Selectively inhibits activation of voltage-gated sodium channels (VGSC) (Nav1.5/SCN5A) in rat cardiac myocytes, followed by shifting activated voltage in a depolarizing direction. The binding site on VGSC is suggested to be site 4 located at the extracellular S3-S4 loop of the channel. Also binds to voltage-gated potassium channels (Kv2.1/KCNB1).<ref>PMID:17150181</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:41, 8 April 2020

OCA — Wed, 08 Apr 2020 07:41:22 GMT

←Older revision		Revision as of 07:41, 8 April 2020
Line 1:		Line 1:

	==Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels==		==Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels==
-	<StructureSection load='2i1t' size='340' side='right' caption='[[2i1t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2i1t' size='340' side='right'caption='[[2i1t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chilobrachys_jingzhao Chilobrachys jingzhao]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2I1T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chilobrachys_jingzhao Chilobrachys jingzhao]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2I1T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [http://pdbe.org/2i1t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [http://www.ebi.ac.uk/pdbsum/2i1t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i1t ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [http://pdbe.org/2i1t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [http://www.ebi.ac.uk/pdbsum/2i1t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i1t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Chilobrachys jingzhao]]		[[Category: Chilobrachys jingzhao]]
		+	[[Category: Large Structures]]
	[[Category: Liang, S]]		[[Category: Liang, S]]
	[[Category: Liao, Z]]		[[Category: Liao, Z]]

OCA at 08:22, 4 July 2018

OCA — Wed, 04 Jul 2018 08:22:21 GMT

←Older revision		Revision as of 08:22, 4 July 2018
Line 1:		Line 1:
		+
	==Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels==		==Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels==
	<StructureSection load='2i1t' size='340' side='right' caption='[[2i1t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2i1t' size='340' side='right' caption='[[2i1t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chilobrachys_guangxiensis~~ Chilobrachys ~~guangxiensis~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I1T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chilobrachys_jingzhao Chilobrachys jingzhao]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I1T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [http://pdbe.org/2i1t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [http://www.ebi.ac.uk/pdbsum/2i1t PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [http://pdbe.org/2i1t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [http://www.ebi.ac.uk/pdbsum/2i1t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i1t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chilobrachys ~~guangxiensis~~]]	+	[[Category: Chilobrachys jingzhao]]
	[[Category: Liang, S]]		[[Category: Liang, S]]
	[[Category: Liao, Z]]		[[Category: Liao, Z]]

OCA at 12:47, 10 September 2015

OCA — Thu, 10 Sep 2015 12:47:43 GMT

←Older revision		Revision as of 12:47, 10 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2i1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chilobrachys_guangxiensis Chilobrachys guangxiensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I1T FirstGlance]. <br>		<table><tr><td colspan='2'>[[2i1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chilobrachys_guangxiensis Chilobrachys guangxiensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I1T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [http://www.ebi.ac.uk/pdbsum/2i1t PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [http://pdbe.org/2i1t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [http://www.ebi.ac.uk/pdbsum/2i1t PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~JZTX3_CHIJI JZTX3_CHIJI~~]] Selectively inhibits activation of voltage-gated sodium channels (VGSC) (Nav1.5/SCN5A) in rat cardiac myocytes, followed by shifting activated voltage in a depolarizing direction. The binding site on VGSC is suggested to be site 4 located at the extracellular S3-S4 loop of the channel. Also binds to voltage-gated potassium channels (Kv2.1/KCNB1).<ref>PMID:17150181</ref>	+	[[http://www.uniprot.org/uniprot/JZTX3_CHIGU JZTX3_CHIGU]] Selectively inhibits activation of voltage-gated sodium channels (VGSC) (Nav1.5/SCN5A) in rat cardiac myocytes, followed by shifting activated voltage in a depolarizing direction. The binding site on VGSC is suggested to be site 4 located at the extracellular S3-S4 loop of the channel. Also binds to voltage-gated potassium channels (Kv2.1/KCNB1).<ref>PMID:17150181</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2i1t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:14, 25 December 2014

OCA — Thu, 25 Dec 2014 00:14:23 GMT

←Older revision		Revision as of 00:14, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [http://www.ebi.ac.uk/pdbsum/2i1t PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [http://www.ebi.ac.uk/pdbsum/2i1t PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/JZTX3_CHIJI JZTX3_CHIJI]] Selectively inhibits activation of voltage-gated sodium channels (VGSC) (Nav1.5/SCN5A) in rat cardiac myocytes, followed by shifting activated voltage in a depolarizing direction. The binding site on VGSC is suggested to be site 4 located at the extracellular S3-S4 loop of the channel. Also binds to voltage-gated potassium channels (Kv2.1/KCNB1).<ref>PMID:17150181</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:31, 22 December 2014

OCA — Mon, 22 Dec 2014 06:31:08 GMT

←Older revision		Revision as of 06:31, 22 December 2014
Line 1:		Line 1:
-	[[~~Image~~:2i1t.~~png\|left\|200px~~]]	+	==Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels==
		+	<StructureSection load='2i1t' size='340' side='right' caption='[[2i1t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2i1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chilobrachys_guangxiensis Chilobrachys guangxiensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I1T FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB], [http://www.ebi.ac.uk/pdbsum/2i1t PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have isolated a cardiotoxin, denoted jingzhaotoxin-III (JZTX-III), from the venom of the Chinese spider Chilobrachys jingzhao. The toxin contains 36 residues stabilized by three intracellular disulfide bridges (I-IV, II-V, and III-VI), assigned by a chemical strategy of partial reduction and sequence analysis. Cloned and sequenced using 3'-rapid amplification of cDNA ends and 5'-rapid amplification of cDNA ends, the full-length cDNA encoded a 63-residue precursor of JZTX-III. Different from other spider peptides, it contains an uncommon endoproteolytic site (-X-Ser-) anterior to mature protein and the intervening regions of 5 residues, which is the smallest in spider toxin cDNAs identified to date. Under whole cell recording, JZTX-III showed no effects on voltage-gated sodium channels (VGSCs) or calcium channels in dorsal root ganglion neurons, whereas it significantly inhibited tetrodotoxin-resistant VGSCs with an IC(50) value of 0.38 microm in rat cardiac myocytes. Different from scorpion beta-toxins, it caused a 10-mV depolarizing shift in the channel activation threshold. The binding site for JZTX-III on VGSCs is further suggested to be site 4 with a simple competitive assay, which at 10 microm eliminated the slowing currents induced by Buthus martensi Karsch I (BMK-I, scorpion alpha-like toxin) completely. JZTX-III shows higher selectivity for VGSC isoforms than other spider toxins affecting VGSCs, and the toxin hopefully represents an important ligand for discriminating cardiac VGSC subtype.

-	~~{{STRUCTURE_2i1t\| PDB=2i1t \| SCENE= }}~~	+	Jingzhaotoxin-III, a novel spider toxin inhibiting activation of voltage-gated sodium channel in rat cardiac myocytes.,Xiao Y, Tang J, Yang Y, Wang M, Hu W, Xie J, Zeng X, Liang S J Biol Chem. 2004 Jun 18;279(25):26220-6. Epub 2004 Apr 14. PMID:15084603<ref>PMID:15084603</ref>

-	~~===Solution structure of Jingzhaotoxin-III~~, a ~~novel toxin inhibiting both Nav and Kv channels===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15084603}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[2i1t]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Chilobrachys_guangxiensis Chilobrachys guangxiensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA].~~	+	</StructureSection>
	[[Category: Chilobrachys guangxiensis]]		[[Category: Chilobrachys guangxiensis]]
-	[[Category: Liang, S.]]	+	[[Category: Liang, S]]
-	[[Category: Liao, Z.]]	+	[[Category: Liao, Z]]
-	[[Category: Peng, K.]]	+	[[Category: Peng, K]]
	[[Category: Cardiac myocyte]]		[[Category: Cardiac myocyte]]
	[[Category: Jingzhaotoxin-iii]]		[[Category: Jingzhaotoxin-iii]]

OCA: Protected "2i1t" [edit=sysop:move=sysop]

OCA — Wed, 24 Oct 2012 08:00:57 GMT

Protected "2i1t" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:00, 24 October 2012

OCA at 08:00, 24 October 2012

OCA — Wed, 24 Oct 2012 08:00:52 GMT

←Older revision		Revision as of 08:00, 24 October 2012
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-	[[Image:2i1t.~~gif~~\|left\|200px]]	+	[[Image:2i1t.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2i1t", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2i1t\| PDB=2i1t \| SCENE= }}		{{STRUCTURE_2i1t\| PDB=2i1t \| SCENE= }}

-	~~'''~~Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels~~'''~~	+	===Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels===

-		+	{{ABSTRACT_PUBMED_15084603}}
-	~~==Overview==~~	+
-	We have isolated a cardiotoxin, denoted jingzhaotoxin-III (JZTX-III), from the venom of the Chinese spider Chilobrachys jingzhao. The toxin contains 36 residues stabilized by three intracellular disulfide bridges (I-IV, II-V, and III-VI), assigned by a chemical strategy of partial reduction and sequence analysis. Cloned and sequenced using 3'-rapid amplification of cDNA ends and 5'-rapid amplification of cDNA ends, the full-length cDNA encoded a 63-residue precursor of JZTX-III. Different from other spider peptides, it contains an uncommon endoproteolytic site (-X-Ser-) anterior to mature protein and the intervening regions of 5 residues, which is the smallest in spider toxin cDNAs identified to date. Under whole cell recording, JZTX-III showed no effects on voltage-gated sodium channels (VGSCs) or calcium channels in dorsal root ganglion neurons, whereas it significantly inhibited tetrodotoxin-resistant VGSCs with an IC(50) value of 0.38 microm in rat cardiac myocytes. Different from scorpion beta-toxins, it caused a 10-mV depolarizing shift in the channel activation threshold. The binding site for JZTX-III on VGSCs is further suggested to be site 4 with a simple competitive assay, which at 10 microm eliminated the slowing currents induced by Buthus martensi Karsch I (BMK-I, scorpion alpha-like toxin) completely. JZTX-III shows higher selectivity for VGSC isoforms than other spider toxins affecting VGSCs, and the toxin hopefully represents an important ligand for discriminating cardiac VGSC subtype.	+

	==About this Structure==		==About this Structure==
-	~~2I1T is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/~~Chilobrachys_jingzhao~~ Chilobrachys ~~jingzhao~~]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA].	+	[[2i1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chilobrachys_guangxiensis Chilobrachys guangxiensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I1T OCA].
-		+	[[Category: Chilobrachys guangxiensis]]
-	~~==Reference==~~	+
-	Jingzhaotoxin-III, a novel spider toxin inhibiting activation of voltage-gated sodium channel in rat cardiac myocytes., Xiao Y, Tang J, Yang Y, Wang M, Hu W, Xie J, Zeng X, Liang S, J Biol Chem. 2004 Jun 18;279(25):26220-6. Epub 2004 Apr 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15084603 15084603]	+
-	[[Category: Chilobrachys ~~jingzhao]]~~	+
-	~~[[Category: Single protein~~]]	+
	[[Category: Liang, S.]]		[[Category: Liang, S.]]
	[[Category: Liao, Z.]]		[[Category: Liao, Z.]]
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	[[Category: Nav channel]]		[[Category: Nav channel]]
	[[Category: Solution structure]]		[[Category: Solution structure]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Sun May 4 06:58:33 2008''~~	+	[[Category: Toxin]]

OCA at 03:58, 4 May 2008

OCA — Sun, 04 May 2008 03:58:33 GMT

←Older revision		Revision as of 03:58, 4 May 2008
Line 1:		Line 1:
	[[Image:2i1t.gif\|left\|200px]]		[[Image:2i1t.gif\|left\|200px]]

-	{{Structure	+	<!--
-	\|PDB~~= 2i1t \|SIZE=350\|CAPTION= <~~scene ~~name='initialview01'>2i1t</scene>~~	+	The line below this paragraph, containing "STRUCTURE_2i1t", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND=~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	\|~~ACTIVITY~~=	+	or leave the SCENE parameter empty for the default display.
-	\|~~GENE~~=	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_2i1t\| PDB=2i1t \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1t OCA], [http://www.ebi.ac.uk/pdbsum/2i1t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i1t RCSB]</span>	+
-	}}	+

	'''Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels'''		'''Solution structure of Jingzhaotoxin-III, a novel toxin inhibiting both Nav and Kv channels'''
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	[[Category: Liao, Z.]]		[[Category: Liao, Z.]]
	[[Category: Peng, K.]]		[[Category: Peng, K.]]
-	[[Category: ~~cardiac~~ myocyte]]	+	[[Category: Cardiac myocyte]]
-	[[Category: ~~jingzhaotoxin~~-iii]]	+	[[Category: Jingzhaotoxin-iii]]
-	[[Category: ~~kv2~~ 1 channel]]	+	[[Category: Kv2 1 channel]]
-	[[Category: ~~nav~~ channel]]	+	[[Category: Nav channel]]
-	[[Category: ~~solution~~ structure]]	+	[[Category: Solution structure]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:58:33 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 03~~:38:32 2008''	+