2i68 - Revision history

OCA at 13:52, 13 March 2024

OCA — Wed, 13 Mar 2024 13:52:43 GMT

←Older revision		Revision as of 13:52, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='2i68' size='340' side='right'caption='[[2i68]], [[Resolution\|resolution]] 7.50Å' scene=''>		<StructureSection load='2i68' size='340' side='right'caption='[[2i68]], [[Resolution\|resolution]] 7.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i68]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I68 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i68]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I68 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~emrE~~, ~~EB, mvrC (~~[~~https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=~~5&~~id=562 "Bacillus coli" Migula 1895])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution\|Resolution]] 7.5Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i68 OCA], [https://pdbe.org/2i68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i68 RCSB], [https://www.ebi.ac.uk/pdbsum/2i68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i68 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i68 OCA], [https://pdbe.org/2i68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i68 RCSB], [https://www.ebi.ac.uk/pdbsum/2i68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i68 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI]] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>	+	[https://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i68 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i68 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Small multidrug resistance (SMR) transporters contribute to bacterial resistance by coupling the efflux of a wide range of toxic aromatic cations, some of which are commonly used as antibiotics and antiseptics, to proton influx. EmrE is a prototypical small multidrug resistance transporter comprising four transmembrane segments (M1-M4) that forms dimers. It was suggested recently that EmrE molecules in the dimer have different topologies, i.e. monomers have opposite orientations with respect to the membrane plane. A 3-D structure of EmrE acquired by electron cryo-microscopy (cryo-EM) at 7.5 Angstroms resolution in the membrane plane showed that parts of the structure are related by quasi-symmetry. We used this symmetry relationship, combined with sequence conservation data, to assign the transmembrane segments in EmrE to the densities seen in the cryo-EM structure. A C alpha model of the transmembrane region was constructed by considering the evolutionary conservation pattern of each helix. The model is validated by much of the biochemical data on EmrE with most of the positions that were identified as affecting substrate translocation being located around the substrate-binding cavity. A suggested mechanism for proton-coupled substrate translocation in small multidrug resistance antiporters provides a mechanistic rationale to the experimentally observed inverted topology.
-
-	Quasi-symmetry in the cryo-EM structure of EmrE provides the key to modeling its transmembrane domain.,Fleishman SJ, Harrington SE, Enosh A, Halperin D, Tate CG, Ben-Tal N J Mol Biol. 2006 Nov 17;364(1):54-67. Epub 2006 Aug 30. PMID:17005200<ref>PMID:17005200</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2i68" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ben-Tal, N]]	+	[[Category: Ben-Tal N]]
-	[[Category: Enosh, A]]	+	[[Category: Enosh A]]
-	[[Category: Fleishman~~, S J~~]]	+	[[Category: Fleishman SJ]]
-	[[Category: Halperin, D]]	+	[[Category: Halperin D]]
-	[[Category: Harrington~~, S E~~]]	+	[[Category: Harrington SE]]
-	[[Category: Tate~~, C G]]~~	+	[[Category: Tate CG]]
-	~~[[Category: Dual topology]]~~	+
-	~~[[Category: Homodimer]]~~	+
-	~~[[Category: Small-multidrug resistance]]~~	+
-	~~[[Category: Transmembrane protein]]~~	+
-	~~[[Category: Transport protein]]~~	+
-	~~[[Category: Transporter~~]]	+

OCA at 07:28, 24 March 2021

OCA — Wed, 24 Mar 2021 07:28:59 GMT

←Older revision		Revision as of 07:28, 24 March 2021
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	==Cryo-EM based theoretical model structure of transmembrane domain of the multidrug-resistance antiporter from E. coli EmrE==		==Cryo-EM based theoretical model structure of transmembrane domain of the multidrug-resistance antiporter from E. coli EmrE==
-	<StructureSection load='2i68' size='340' side='right' caption='[[2i68]], [[Resolution\|resolution]] 7.50Å' scene=''>	+	<StructureSection load='2i68' size='340' side='right'caption='[[2i68]], [[Resolution\|resolution]] 7.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i68]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2I68 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i68]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I68 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, EB, mvrC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, EB, mvrC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i68 OCA], [~~http~~://pdbe.org/2i68 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2i68 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2i68 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2i68 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i68 OCA], [https://pdbe.org/2i68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i68 RCSB], [https://www.ebi.ac.uk/pdbsum/2i68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i68 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI]] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>	+	[[https://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI]] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Ben-Tal, N]]		[[Category: Ben-Tal, N]]
	[[Category: Enosh, A]]		[[Category: Enosh, A]]

OCA at 08:39, 4 July 2018

OCA — Wed, 04 Jul 2018 08:39:41 GMT

←Older revision		Revision as of 08:39, 4 July 2018
Line 1:		Line 1:
		+
	==Cryo-EM based theoretical model structure of transmembrane domain of the multidrug-resistance antiporter from E. coli EmrE==		==Cryo-EM based theoretical model structure of transmembrane domain of the multidrug-resistance antiporter from E. coli EmrE==
-	<StructureSection load='2i68' size='340' side='right' caption='[[2i68]]' scene=''>	+	<StructureSection load='2i68' size='340' side='right' caption='[[2i68]], [[Resolution\|resolution]] 7.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2i68]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I68 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2i68]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I68 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, EB, mvrC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, EB, mvrC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i68 OCA], [http://pdbe.org/2i68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i68 RCSB], [http://www.ebi.ac.uk/pdbsum/2i68 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i68 OCA], [http://pdbe.org/2i68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i68 RCSB], [http://www.ebi.ac.uk/pdbsum/2i68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i68 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/2i68_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/2i68_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i68 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:05, 11 September 2015

OCA — Fri, 11 Sep 2015 11:05:24 GMT

←Older revision		Revision as of 11:05, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2i68' size='340' side='right' caption='[[2i68]]' scene=''>		<StructureSection load='2i68' size='340' side='right' caption='[[2i68]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i68]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I68 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i68]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I68 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, EB, mvrC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, EB, mvrC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i68 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i68 RCSB], [http://www.ebi.ac.uk/pdbsum/2i68 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i68 OCA], [http://pdbe.org/2i68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i68 RCSB], [http://www.ebi.ac.uk/pdbsum/2i68 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2i68" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Ben-Tal, N]]		[[Category: Ben-Tal, N]]
	[[Category: Enosh, A]]		[[Category: Enosh, A]]

OCA at 15:36, 25 December 2014

OCA — Thu, 25 Dec 2014 15:36:03 GMT

←Older revision		Revision as of 15:36, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2i68]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I68 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2i68]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I68 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, EB, mvrC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, EB, mvrC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i68 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i68 RCSB], [http://www.ebi.ac.uk/pdbsum/2i68 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i68 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i68 RCSB], [http://www.ebi.ac.uk/pdbsum/2i68 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI]] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Ben-Tal, N.]]	+	[[Category: Ben-Tal, N]]
-	[[Category: Enosh, A.]]	+	[[Category: Enosh, A]]
-	[[Category: Fleishman, S J.]]	+	[[Category: Fleishman, S J]]
-	[[Category: Halperin, D.]]	+	[[Category: Halperin, D]]
-	[[Category: Harrington, S E.]]	+	[[Category: Harrington, S E]]
-	[[Category: Tate, C G.]]	+	[[Category: Tate, C G]]
	[[Category: Dual topology]]		[[Category: Dual topology]]
	[[Category: Homodimer]]		[[Category: Homodimer]]

OCA at 08:21, 30 September 2014

OCA — Tue, 30 Sep 2014 08:21:45 GMT

←Older revision		Revision as of 08:21, 30 September 2014
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-	[[~~Image~~:2i68.~~png\|left~~\|200px]]	+	==Cryo-EM based theoretical model structure of transmembrane domain of the multidrug-resistance antiporter from E. coli EmrE==
		+	<StructureSection load='2i68' size='340' side='right' caption='[[2i68]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2i68]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I68 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">emrE, EB, mvrC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i68 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i68 RCSB], [http://www.ebi.ac.uk/pdbsum/2i68 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/2i68_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Small multidrug resistance (SMR) transporters contribute to bacterial resistance by coupling the efflux of a wide range of toxic aromatic cations, some of which are commonly used as antibiotics and antiseptics, to proton influx. EmrE is a prototypical small multidrug resistance transporter comprising four transmembrane segments (M1-M4) that forms dimers. It was suggested recently that EmrE molecules in the dimer have different topologies, i.e. monomers have opposite orientations with respect to the membrane plane. A 3-D structure of EmrE acquired by electron cryo-microscopy (cryo-EM) at 7.5 Angstroms resolution in the membrane plane showed that parts of the structure are related by quasi-symmetry. We used this symmetry relationship, combined with sequence conservation data, to assign the transmembrane segments in EmrE to the densities seen in the cryo-EM structure. A C alpha model of the transmembrane region was constructed by considering the evolutionary conservation pattern of each helix. The model is validated by much of the biochemical data on EmrE with most of the positions that were identified as affecting substrate translocation being located around the substrate-binding cavity. A suggested mechanism for proton-coupled substrate translocation in small multidrug resistance antiporters provides a mechanistic rationale to the experimentally observed inverted topology.

-	~~{{STRUCTURE_2i68\| PDB=2i68 \| SCENE= }}~~	+	Quasi-symmetry in the cryo-EM structure of EmrE provides the key to modeling its transmembrane domain.,Fleishman SJ, Harrington SE, Enosh A, Halperin D, Tate CG, Ben-Tal N J Mol Biol. 2006 Nov 17;364(1):54-67. Epub 2006 Aug 30. PMID:17005200<ref>PMID:17005200</ref>

-	~~===Cryo-EM based theoretical model structure of transmembrane domain~~ of the ~~multidrug-resistance antiporter from E~~. ~~coli EmrE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17005200}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2i68]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017005200<~~/~~ref~~><~~ref group="xtra">PMID:014633977</ref><references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ben-Tal, N.]]		[[Category: Ben-Tal, N.]]

OCA at 13:21, 30 January 2013

OCA — Wed, 30 Jan 2013 13:21:46 GMT

←Older revision		Revision as of 13:21, 30 January 2013
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	[[Image:2i68.png\|left\|200px]]		[[Image:2i68.png\|left\|200px]]

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	{{STRUCTURE_2i68\| PDB=2i68 \| SCENE= }}		{{STRUCTURE_2i68\| PDB=2i68 \| SCENE= }}

	===Cryo-EM based theoretical model structure of transmembrane domain of the multidrug-resistance antiporter from E. coli EmrE===		===Cryo-EM based theoretical model structure of transmembrane domain of the multidrug-resistance antiporter from E. coli EmrE===

-
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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17005200~~</ref><ref group="xtra">PMID:~~14633977~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017005200</ref><ref group="xtra">PMID:014633977</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ben-Tal, N.]]		[[Category: Ben-Tal, N.]]

OCA: Protected "2i68" [edit=sysop:move=sysop]

OCA — Mon, 14 Mar 2011 19:26:11 GMT

Protected "2i68" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:26, 14 March 2011

OCA at 19:26, 14 March 2011

OCA — Mon, 14 Mar 2011 19:26:09 GMT

←Older revision		Revision as of 19:26, 14 March 2011
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-	{{Seed}}
	[[Image:2i68.png\|left\|200px]]		[[Image:2i68.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~2I68~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA].	+	[[2i68]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA].

	==Reference==		==Reference==
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	[[Category: Small-multidrug resistance]]		[[Category: Small-multidrug resistance]]
	[[Category: Transmembrane protein]]		[[Category: Transmembrane protein]]
		+	[[Category: Transport protein]]
	[[Category: Transporter]]		[[Category: Transporter]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:34:52 2009''

OCA at 18:34, 17 February 2009

OCA — Tue, 17 Feb 2009 18:34:55 GMT

←Older revision		Revision as of 18:34, 17 February 2009
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	==About this Structure==		==About this Structure==
-	2I68 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA].	+	2I68 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I68 OCA].

	==Reference==		==Reference==
-	Quasi-symmetry in the cryo-EM structure of EmrE provides the key to modeling its transmembrane domain., Fleishman SJ, Harrington SE, Enosh A, Halperin D, Tate CG, Ben-Tal N, J Mol Biol. 2006 Nov 17;364(1):54-67. Epub 2006 Aug 30. PMID:~~[http:~~/~~/www.ncbi.nlm.nih.gov/pubmed/17005200 17005200]~~	+	<ref group="xtra">PMID:17005200</ref><ref group="xtra">PMID:14633977</ref><references group="xtra"/>
-		+
-	Three-dimensional structure of the bacterial multidrug transporter EmrE shows it is an asymmetric homodimer., Ubarretxena-Belandia I, Baldwin JM, Schuldiner S, Tate CG, EMBO J. 2003 Dec 1;22(23):6175-81. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/14633977 14633977]~~	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Ben-Tal, N.]]		[[Category: Ben-Tal, N.]]
	[[Category: Enosh, A.]]		[[Category: Enosh, A.]]
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	[[Category: Transporter]]		[[Category: Transporter]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 04~~:28:~~30 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:34:52 2009''