2i6y - Revision history

OCA at 09:01, 21 February 2024

OCA — Wed, 21 Feb 2024 09:01:57 GMT

←Older revision		Revision as of 09:01, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='2i6y' size='340' side='right'caption='[[2i6y]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2i6y' size='340' side='right'caption='[[2i6y]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i6y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus~~ tuberculosis~~" (zopf 1883) klein 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I6Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i6y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I6Y FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>mbtI, MT2454, Rv2386c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1773~~ "~~Bacillus tuberculosis~~" ~~(Zopf 1883) Klein 1884])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'~~>[~~https://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase~~]~~, with EC number [https://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [https://pdbe.org/2i6y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB], [https://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i6y ProSAT], [https://www.topsan.org/Proteins/TBSGC/2i6y TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [https://pdbe.org/2i6y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB], [https://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i6y ProSAT], [https://www.topsan.org/Proteins/TBSGC/2i6y TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MBTI_MYCTU MBTI_MYCTU]] Mediates the production of salicylate from chorismate via an isochorismate intermediate. Presents both isochorismate synthase and isochorismate-pyruvate lyase activities. Salycilate is the starter unit in the production of the virulence-conferring salicylate-based siderophore mycobactin.<ref>PMID:16923875</ref>	+	[https://www.uniprot.org/uniprot/MBTI_MYCTU MBTI_MYCTU] Mediates the production of salicylate from chorismate via an isochorismate intermediate. Presents both isochorismate synthase and isochorismate-pyruvate lyase activities. Salycilate is the starter unit in the production of the virulence-conferring salicylate-based siderophore mycobactin.<ref>PMID:16923875</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i6y ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i6y ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	MbtI (rv2386c) from Mycobacterium tuberculosis catalyzes the initial transformation in mycobactin biosynthesis by converting chorismate to salicylate. We report here the structure of MbtI at 2.5 A resolution and demonstrate that isochorismate is a kinetically competent intermediate in the synthesis of salicylate from chorismate. At pH values below 7.5 isochorismate is the dominant product while above this pH value the enzyme converts chorismate to salicylate without the accumulation of isochorismate in solution. The salicylate and isochorismate synthase activities of MbtI are Mg2+-dependent, and in the absence of Mg2+ MbtI has a promiscuous chorismate mutase activity similar to that of the isochorismate pyruvate lyase, PchB, from Pseudomonas aeruginosa. MbtI is part of a larger family of chorismate-binding enzymes descended from a common ancestor (the MST family), that includes the isochorismate synthases and anthranilate synthases. The lack of active site residues unique to pyruvate eliminating members of this family, combined with the observed chorismate mutase activity, suggests that MbtI may exploit a sigmatropic pyruvate elimination mechanism similar to that proposed for PchB. Using a combination of structural, kinetic, and sequence based studies we propose a mechanism for MbtI applicable to all members of the MST enzyme family.
-
-	Structure and mechanism of MbtI, the salicylate synthase from Mycobacterium tuberculosis.,Zwahlen J, Kolappan S, Zhou R, Kisker C, Tonge PJ Biochemistry. 2007 Jan 30;46(4):954-64. PMID:17240979<ref>PMID:17240979</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2i6y" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Anthranilate synthase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kisker, C]]	+	[[Category: Mycobacterium tuberculosis]]
-	[[Category: Subramaniapillai, K]]	+	[[Category: Kisker C]]
-	[[Category: Tonge~~, P J~~]]	+	[[Category: Subramaniapillai K]]
-	[[Category: Zhou, R]]	+	[[Category: Tonge PJ]]
-	[[Category: Zwahlen, J]]	+	[[Category: Zhou R]]
-	~~[[Category: Beta sheet]]~~	+	[[Category: Zwahlen J]]
-	~~[[Category: Lyase~~]]	+

OCA at 07:29, 24 March 2021

OCA — Wed, 24 Mar 2021 07:29:29 GMT

←Older revision		Revision as of 07:29, 24 March 2021
Line 1:		Line 1:

	==Structure and Mechanism of Mycobacterium tuberculosis Salicylate Synthase, MbtI==		==Structure and Mechanism of Mycobacterium tuberculosis Salicylate Synthase, MbtI==
-	<StructureSection load='2i6y' size='340' side='right' caption='[[2i6y]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='2i6y' size='340' side='right'caption='[[2i6y]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i6y]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2I6Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i6y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I6Y FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mbtI, MT2454, Rv2386c ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mbtI, MT2454, Rv2386c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [~~http~~://pdbe.org/2i6y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2i6y ProSAT], [~~http~~://www.topsan.org/Proteins/TBSGC/2i6y TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [https://pdbe.org/2i6y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB], [https://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i6y ProSAT], [https://www.topsan.org/Proteins/TBSGC/2i6y TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MBTI_MYCTU MBTI_MYCTU]] Mediates the production of salicylate from chorismate via an isochorismate intermediate. Presents both isochorismate synthase and isochorismate-pyruvate lyase activities. Salycilate is the starter unit in the production of the virulence-conferring salicylate-based siderophore mycobactin.<ref>PMID:16923875</ref>	+	[[https://www.uniprot.org/uniprot/MBTI_MYCTU MBTI_MYCTU]] Mediates the production of salicylate from chorismate via an isochorismate intermediate. Presents both isochorismate synthase and isochorismate-pyruvate lyase activities. Salycilate is the starter unit in the production of the virulence-conferring salicylate-based siderophore mycobactin.<ref>PMID:16923875</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/2i6y_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/2i6y_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Anthranilate synthase]]		[[Category: Anthranilate synthase]]
		+	[[Category: Large Structures]]
	[[Category: Kisker, C]]		[[Category: Kisker, C]]
	[[Category: Subramaniapillai, K]]		[[Category: Subramaniapillai, K]]

OCA at 08:24, 18 October 2017

OCA — Wed, 18 Oct 2017 08:24:19 GMT

←Older revision		Revision as of 08:24, 18 October 2017
Line 1:		Line 1:
		+
	==Structure and Mechanism of Mycobacterium tuberculosis Salicylate Synthase, MbtI==		==Structure and Mechanism of Mycobacterium tuberculosis Salicylate Synthase, MbtI==
	<StructureSection load='2i6y' size='340' side='right' caption='[[2i6y]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2i6y' size='340' side='right' caption='[[2i6y]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mbtI, MT2454, Rv2386c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mbtI, MT2454, Rv2386c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [http://pdbe.org/2i6y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB], [http://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [http://www.topsan.org/Proteins/TBSGC/2i6y TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [http://pdbe.org/2i6y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB], [http://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i6y ProSAT], [http://www.topsan.org/Proteins/TBSGC/2i6y TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 13:14, 8 February 2016

OCA — Mon, 08 Feb 2016 13:14:53 GMT

←Older revision		Revision as of 13:14, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i6y ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:55, 10 September 2015

OCA — Thu, 10 Sep 2015 11:55:35 GMT

←Older revision		Revision as of 11:55, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2i6y' size='340' side='right' caption='[[2i6y]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2i6y' size='340' side='right' caption='[[2i6y]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2i6y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis Mycobacterium~~ tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I6Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2i6y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I6Y FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mbtI, MT2454, Rv2386c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 ~~Mycobacterium~~ tuberculosis])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mbtI, MT2454, Rv2386c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB], [http://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [http://www.topsan.org/Proteins/TBSGC/2i6y TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [http://pdbe.org/2i6y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB], [http://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [http://www.topsan.org/Proteins/TBSGC/2i6y TOPSAN]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MBTI_MYCTU MBTI_MYCTU]] Mediates the production of salicylate from chorismate via an isochorismate intermediate. Presents both isochorismate synthase and isochorismate-pyruvate lyase activities. Salycilate is the starter unit in the production of the virulence-conferring salicylate-based siderophore mycobactin.<ref>PMID:16923875</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 25:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2i6y" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Anthranilate synthase]]		[[Category: Anthranilate synthase]]
-	[[Category: Mycobacterium tuberculosis]]
	[[Category: Kisker, C]]		[[Category: Kisker, C]]
	[[Category: Subramaniapillai, K]]		[[Category: Subramaniapillai, K]]

OCA at 08:21, 16 January 2015

OCA — Fri, 16 Jan 2015 08:21:36 GMT

←Older revision		Revision as of 08:21, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2i6y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I6Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[2i6y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I6Y FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mbtI, MT2454, Rv2386c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mbtI, MT2454, Rv2386c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB], [http://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [http://www.topsan.org/Proteins/TBSGC/2i6y TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB], [http://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [http://www.topsan.org/Proteins/TBSGC/2i6y TOPSAN]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	[[Category: Anthranilate synthase]]		[[Category: Anthranilate synthase]]
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
-	[[Category: Kisker, C.]]	+	[[Category: Kisker, C]]
-	[[Category: Subramaniapillai, K.]]	+	[[Category: Subramaniapillai, K]]
-	[[Category: Tonge, P J.]]	+	[[Category: Tonge, P J]]
-	[[Category: Zhou, R.]]	+	[[Category: Zhou, R]]
-	[[Category: Zwahlen, J.]]	+	[[Category: Zwahlen, J]]
	[[Category: Beta sheet]]		[[Category: Beta sheet]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 09:32, 30 September 2014

OCA — Tue, 30 Sep 2014 09:32:45 GMT

←Older revision		Revision as of 09:32, 30 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2i6y.~~png\|left~~\|200px]]	+	==Structure and Mechanism of Mycobacterium tuberculosis Salicylate Synthase, MbtI==
		+	<StructureSection load='2i6y' size='340' side='right' caption='[[2i6y]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2i6y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I6Y FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mbtI, MT2454, Rv2386c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Anthranilate_synthase Anthranilate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.27 4.1.3.27] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i6y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2i6y RCSB], [http://www.ebi.ac.uk/pdbsum/2i6y PDBsum], [http://www.topsan.org/Proteins/TBSGC/2i6y TOPSAN]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/2i6y_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MbtI (rv2386c) from Mycobacterium tuberculosis catalyzes the initial transformation in mycobactin biosynthesis by converting chorismate to salicylate. We report here the structure of MbtI at 2.5 A resolution and demonstrate that isochorismate is a kinetically competent intermediate in the synthesis of salicylate from chorismate. At pH values below 7.5 isochorismate is the dominant product while above this pH value the enzyme converts chorismate to salicylate without the accumulation of isochorismate in solution. The salicylate and isochorismate synthase activities of MbtI are Mg2+-dependent, and in the absence of Mg2+ MbtI has a promiscuous chorismate mutase activity similar to that of the isochorismate pyruvate lyase, PchB, from Pseudomonas aeruginosa. MbtI is part of a larger family of chorismate-binding enzymes descended from a common ancestor (the MST family), that includes the isochorismate synthases and anthranilate synthases. The lack of active site residues unique to pyruvate eliminating members of this family, combined with the observed chorismate mutase activity, suggests that MbtI may exploit a sigmatropic pyruvate elimination mechanism similar to that proposed for PchB. Using a combination of structural, kinetic, and sequence based studies we propose a mechanism for MbtI applicable to all members of the MST enzyme family.

-	~~{{STRUCTURE_2i6y\| PDB=2i6y \| SCENE= }}~~	+	Structure and mechanism of MbtI, the salicylate synthase from Mycobacterium tuberculosis.,Zwahlen J, Kolappan S, Zhou R, Kisker C, Tonge PJ Biochemistry. 2007 Jan 30;46(4):954-64. PMID:17240979<ref>PMID:17240979</ref>

-	~~===Structure and Mechanism of Mycobacterium tuberculosis Salicylate Synthase~~, ~~MbtI===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17240979}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2i6y]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017240979<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Anthranilate synthase]]		[[Category: Anthranilate synthase]]
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]

OCA: Protected "2i6y" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 11:47:49 GMT

Protected "2i6y" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:47, 7 January 2013

OCA at 11:47, 7 January 2013

OCA — Mon, 07 Jan 2013 11:47:45 GMT

←Older revision		Revision as of 11:47, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2i6y.png\|left\|200px]]		[[Image:2i6y.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2i6y", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2i6y\| PDB=2i6y \| SCENE= }}		{{STRUCTURE_2i6y\| PDB=2i6y \| SCENE= }}

	===Structure and Mechanism of Mycobacterium tuberculosis Salicylate Synthase, MbtI===		===Structure and Mechanism of Mycobacterium tuberculosis Salicylate Synthase, MbtI===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17240979}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17240979 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17240979}}		{{ABSTRACT_PUBMED_17240979}}

	==About this Structure==		==About this Structure==
-	~~2I6Y~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA].	+	[[2i6y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17240979~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017240979</ref><references group="xtra"/>
	[[Category: Anthranilate synthase]]		[[Category: Anthranilate synthase]]
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
Line 32:		Line 20:
	[[Category: Zwahlen, J.]]		[[Category: Zwahlen, J.]]
	[[Category: Beta sheet]]		[[Category: Beta sheet]]
-		+	[[Category: Lyase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 22:20:51 2009''~~	+

OCA at 20:20, 16 February 2009

OCA — Mon, 16 Feb 2009 20:20:56 GMT

←Older revision		Revision as of 20:20, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2I6Y is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA].	+	2I6Y is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Y OCA].

	==Reference==		==Reference==
-	~~Structure and mechanism of MbtI, the salicylate synthase from Mycobacterium tuberculosis., Zwahlen J, Kolappan S, Zhou R, Kisker C, Tonge PJ, Biochemistry. 2007 Jan 30;46(4):954-64.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17240979 17240979]~~	+	<ref group="xtra">PMID:17240979</ref><references group="xtra"/>
	[[Category: Anthranilate synthase]]		[[Category: Anthranilate synthase]]
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
-	[[Category: Single protein]]
	[[Category: Kisker, C.]]		[[Category: Kisker, C.]]
	[[Category: Subramaniapillai, K.]]		[[Category: Subramaniapillai, K.]]
Line 34:		Line 33:
	[[Category: Beta sheet]]		[[Category: Beta sheet]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon ~~Jul 28 03~~:21:~~15 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:20:51 2009''