2iov - Revision history

OCA at 08:53, 25 October 2023

OCA — Wed, 25 Oct 2023 08:53:34 GMT

←Older revision		Revision as of 08:53, 25 October 2023
Line 3:		Line 3:
	<StructureSection load='2iov' size='340' side='right'caption='[[2iov]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2iov' size='340' side='right'caption='[[2iov]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2iov]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp.~~_sc22~~ Echinophyllia sp. ~~sc22~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IOV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2iov]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._SC22 Echinophyllia sp. SC22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IOV FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dronpa ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iov OCA], [https://pdbe.org/2iov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iov RCSB], [https://www.ebi.ac.uk/pdbsum/2iov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iov ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iov OCA], [https://pdbe.org/2iov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iov RCSB], [https://www.ebi.ac.uk/pdbsum/2iov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iov ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5TLG6_9CNID Q5TLG6_9CNID]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Echinophyllia sp. ~~sc22~~]]	+	[[Category: Echinophyllia sp. SC22]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Andresen, M]]	+	[[Category: Andresen M]]
-	[[Category: Eggeling, C]]	+	[[Category: Eggeling C]]
-	[[Category: Hell~~, S W~~]]	+	[[Category: Hell SW]]
-	[[Category: Jakobs, S]]	+	[[Category: Jakobs S]]
-	[[Category: Stiel~~, A C~~]]	+	[[Category: Stiel AC]]
-	[[Category: Trowitzsch, S]]	+	[[Category: Trowitzsch S]]
-	[[Category: Wahl~~, M C~~]]	+	[[Category: Wahl MC]]
-	[[Category: Weber, G]]	+	[[Category: Weber G]]
-	~~[[Category: Green-fluorescent protein-like protein]]~~	+
-	~~[[Category: Luminescent protein]]~~	+
-	~~[[Category: Reversibly switchable fluorescent protein~~]]	+

OCA at 11:20, 31 March 2021

OCA — Wed, 31 Mar 2021 11:20:14 GMT

←Older revision		Revision as of 11:20, 31 March 2021
Line 1:		Line 1:

	==Bright-state structure of the reversibly switchable fluorescent protein Dronpa==		==Bright-state structure of the reversibly switchable fluorescent protein Dronpa==
-	<StructureSection load='2iov' size='340' side='right' caption='[[2iov]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='2iov' size='340' side='right'caption='[[2iov]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2iov]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2IOV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2iov]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IOV FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dronpa ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dronpa ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2iov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iov OCA], [~~http~~://pdbe.org/2iov PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2iov RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2iov PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2iov ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iov OCA], [https://pdbe.org/2iov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iov RCSB], [https://www.ebi.ac.uk/pdbsum/2iov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iov ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/io/2iov_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/io/2iov_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 2iov" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2iov" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dronpa\|Dronpa]]
		+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Echinophyllia sp. sc22]]		[[Category: Echinophyllia sp. sc22]]
		+	[[Category: Large Structures]]
	[[Category: Andresen, M]]		[[Category: Andresen, M]]
	[[Category: Eggeling, C]]		[[Category: Eggeling, C]]

OCA at 08:33, 18 October 2017

OCA — Wed, 18 Oct 2017 08:33:29 GMT

←Older revision		Revision as of 08:33, 18 October 2017
Line 1:		Line 1:
		+
	==Bright-state structure of the reversibly switchable fluorescent protein Dronpa==		==Bright-state structure of the reversibly switchable fluorescent protein Dronpa==
	<StructureSection load='2iov' size='340' side='right' caption='[[2iov]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2iov' size='340' side='right' caption='[[2iov]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dronpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dronpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iov OCA], [http://pdbe.org/2iov PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iov RCSB], [http://www.ebi.ac.uk/pdbsum/2iov PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iov OCA], [http://pdbe.org/2iov PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iov RCSB], [http://www.ebi.ac.uk/pdbsum/2iov PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2iov ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iov ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 2iov" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2iov" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Dronpa\|Dronpa]]
-	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:37, 11 September 2015

OCA — Fri, 11 Sep 2015 11:37:08 GMT

←Older revision		Revision as of 11:37, 11 September 2015
Line 5:		Line 5:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dronpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dronpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iov OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iov RCSB], [http://www.ebi.ac.uk/pdbsum/2iov PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iov OCA], [http://pdbe.org/2iov PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iov RCSB], [http://www.ebi.ac.uk/pdbsum/2iov PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2iov" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 09:21, 16 January 2015

OCA — Fri, 16 Jan 2015 09:21:14 GMT

←Older revision		Revision as of 09:21, 16 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2iov]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IOV FirstGlance]. <br>		<table><tr><td colspan='2'>[[2iov]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IOV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dronpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dronpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iov OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iov RCSB], [http://www.ebi.ac.uk/pdbsum/2iov PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iov OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iov RCSB], [http://www.ebi.ac.uk/pdbsum/2iov PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Echinophyllia sp. sc22]]		[[Category: Echinophyllia sp. sc22]]
-	[[Category: Andresen, M.]]	+	[[Category: Andresen, M]]
-	[[Category: Eggeling, C.]]	+	[[Category: Eggeling, C]]
-	[[Category: Hell, S W.]]	+	[[Category: Hell, S W]]
-	[[Category: Jakobs, S.]]	+	[[Category: Jakobs, S]]
-	[[Category: Stiel, A C.]]	+	[[Category: Stiel, A C]]
-	[[Category: Trowitzsch, S.]]	+	[[Category: Trowitzsch, S]]
-	[[Category: Wahl, M C.]]	+	[[Category: Wahl, M C]]
-	[[Category: Weber, G.]]	+	[[Category: Weber, G]]
	[[Category: Green-fluorescent protein-like protein]]		[[Category: Green-fluorescent protein-like protein]]
	[[Category: Luminescent protein]]		[[Category: Luminescent protein]]
	[[Category: Reversibly switchable fluorescent protein]]		[[Category: Reversibly switchable fluorescent protein]]

OCA at 05:33, 3 October 2014

OCA — Fri, 03 Oct 2014 05:33:22 GMT

←Older revision		Revision as of 05:33, 3 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2iov.~~png~~\|~~left~~\|200px]]	+	==Bright-state structure of the reversibly switchable fluorescent protein Dronpa==
		+	<StructureSection load='2iov' size='340' side='right' caption='[[2iov]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2iov]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IOV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dronpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iov OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iov RCSB], [http://www.ebi.ac.uk/pdbsum/2iov PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/io/2iov_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RSFPs (reversibly switchable fluorescent proteins) may be repeatedly converted between a fluorescent and a non-fluorescent state by irradiation and have attracted widespread interest for many new applications. The RSFP Dronpa may be switched with blue light from a fluorescent state into a non-fluorescent state, and back again with UV light. To obtain insight into the underlying molecular mechanism of this switching, we have determined the crystal structure of the fluorescent equilibrium state of Dronpa. Its bicyclic chromophore is formed spontaneously from the Cys62-Tyr63-Gly64 tripeptide. In the fluorescent state, it adopts a slightly non-coplanar cis conformation within the interior of a typical GFP (green fluorescent protein) b-can fold. Dronpa shares some structural features with asFP595, another RSFP whose chromophore has previously been demonstrated to undergo a cis-trans isomerization upon photoswitching. Based on the structural comparison with asFP595, we have generated new Dronpa variants with an up to more than 1000-fold accelerated switching behaviour. The mutations which were introduced at position Val157 or Met159 apparently reduce the steric hindrance for a cis-trans isomerization of the chromophore, thus lowering the energy barrier for the blue light-driven on-to-off transition. The findings reported in the present study support the view that a cis-trans isomerization is one of the key events common to the switching mechanism in RSFPs.

-	~~{{STRUCTURE_2iov\| PDB=2iov \| SCENE= }}~~	+	1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants.,Stiel AC, Trowitzsch S, Weber G, Andresen M, Eggeling C, Hell SW, Jakobs S, Wahl MC Biochem J. 2007 Feb 15;402(1):35-42. PMID:17117927<ref>PMID:17117927</ref>

-	~~===Bright-state structure~~ of the ~~reversibly switchable fluorescent protein Dronpa===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17117927}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2iov]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Echinophyllia_sp~~.~~_sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2IOV OCA].~~	+

	==See Also==		==See Also==
-	*[[Alyssa Marsico/Sandbox 1\|Alyssa Marsico/Sandbox 1]]
-	*[[Devon McCarthy/Sandbox 1\|Devon McCarthy/Sandbox 1]]
	*[[Dronpa\|Dronpa]]		*[[Dronpa\|Dronpa]]
	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]		*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
-	*[[Sandbox104\|Sandbox104]]	+	== References ==
-	*[[User:Joanne Lau/Sandbox 5\|User:Joanne Lau/Sandbox 5]]	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:017117927<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Echinophyllia sp. sc22]]		[[Category: Echinophyllia sp. sc22]]
	[[Category: Andresen, M.]]		[[Category: Andresen, M.]]

OCA at 18:36, 20 October 2012

OCA — Sat, 20 Oct 2012 18:36:21 GMT

←Older revision		Revision as of 18:36, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2iov]] is a 4 chain structure ~~of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Dronpa]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]]~~ with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOV OCA].	+	[[2iov]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOV OCA].

	==See Also==		==See Also==

OCA: Protected "2iov" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 04:37:04 GMT

Protected "2iov" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:37, 27 July 2012

OCA at 04:37, 27 July 2012

OCA — Fri, 27 Jul 2012 04:37:03 GMT

←Older revision		Revision as of 04:37, 27 July 2012
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-	{{Seed}}
	[[Image:2iov.png\|left\|200px]]		[[Image:2iov.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2iov", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_2iov\| PDB=2iov \| SCENE= }}		{{STRUCTURE_2iov\| PDB=2iov \| SCENE= }}

	===Bright-state structure of the reversibly switchable fluorescent protein Dronpa===		===Bright-state structure of the reversibly switchable fluorescent protein Dronpa===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17117927}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17117927 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17117927}}		{{ABSTRACT_PUBMED_17117927}}

	==About this Structure==		==About this Structure==
-	~~2IOV~~ is a 4 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOV OCA].	+	[[2iov]] is a 4 chain structure of [[Alyssa Marsico/Sandbox 1]], [[Devon McCarthy/Sandbox 1]], [[Dronpa]], [[Green Fluorescent Protein]], [[Sandbox104]] and [[User:Joanne Lau/Sandbox 5]] with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IOV OCA].
		+
		+	==See Also==
		+	*[[Alyssa Marsico/Sandbox 1\|Alyssa Marsico/Sandbox 1]]
		+	*[[Devon McCarthy/Sandbox 1\|Devon McCarthy/Sandbox 1]]
		+	*[[Dronpa\|Dronpa]]
		+	*[[Green Fluorescent Protein\|Green Fluorescent Protein]]
		+	*[[Sandbox104\|Sandbox104]]
		+	*[[User:Joanne Lau/Sandbox 5\|User:Joanne Lau/Sandbox 5]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17117927~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017117927</ref><references group="xtra"/>
	[[Category: Echinophyllia sp. sc22]]		[[Category: Echinophyllia sp. sc22]]
	[[Category: Andresen, M.]]		[[Category: Andresen, M.]]
Line 36:		Line 32:
	[[Category: Luminescent protein]]		[[Category: Luminescent protein]]
	[[Category: Reversibly switchable fluorescent protein]]		[[Category: Reversibly switchable fluorescent protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 1 09:49:40 2009''

OCA at 06:49, 1 July 2009

OCA — Wed, 01 Jul 2009 06:49:43 GMT

←Older revision		Revision as of 06:49, 1 July 2009
Line 34:		Line 34:
	[[Category: Weber, G.]]		[[Category: Weber, G.]]
	[[Category: Green-fluorescent protein-like protein]]		[[Category: Green-fluorescent protein-like protein]]
		+	[[Category: Luminescent protein]]
	[[Category: Reversibly switchable fluorescent protein]]		[[Category: Reversibly switchable fluorescent protein]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Feb 17 21~~:03:44 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 1 09:49:40 2009''