2izl - Revision history

OCA at 09:04, 21 February 2024

OCA — Wed, 21 Feb 2024 09:04:35 GMT

←Older revision		Revision as of 09:04, 21 February 2024
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	<StructureSection load='2izl' size='340' side='right'caption='[[2izl]], [[Resolution\|resolution]] 1.48Å' scene=''>		<StructureSection load='2izl' size='340' side='right'caption='[[2izl]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2izl]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2IZL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2izl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IZL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMI:2-IMINOBIOTIN'>IMI</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.48Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2izl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izl OCA], [~~http~~://pdbe.org/2izl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2izl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2izl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2izl ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMI:2-IMINOBIOTIN'>IMI</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2izl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izl OCA], [https://pdbe.org/2izl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2izl RCSB], [https://www.ebi.ac.uk/pdbsum/2izl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2izl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2izl ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2izl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The remarkable stability of the streptavidin tetramer towards subunit dissociation becomes even greater upon binding of biotin. At two equivalent extensive monomer-monomer interfaces, monomers tightly associate into dimers that in turn associate into the tetramer at a less extensive dimer-dimer interface. To probe the structural basis for the enhancement of the stability of streptavidin by biotin, the crystal structures of apostreptavidin and its complexes with biotin and other small molecule and cyclic peptide ligands were determined and compared at resolutions as high as 1.36 A over a range of pH values from as low as 1.39. At low pH dramatic changes occur in the conformation and intersubunit hydrogen bonds involving the loop comprising Asp61 to Ser69. The hydrogen-bonded salt bridge between Asp61 Odelta2 and His87 Ndelta1, observed at higher pH, is replaced with a strong hydrogen bond between Asp61 Odelta1 and Asn85 Odelta1. Through crystallography at multiple pH values, the pH where this conformational change occurs, and thus the pKa of Asp61, was determined in crystals of space group I222 and/or I4122 of apostreptavidin and complexes. A range in pKa values for Asp61 was observed in these structures, the lowest being 1.78+/-0.19 for I222 streptavidin-biotin in 2.9 M (NH4)2SO4. At low pH the decrease in pKa of Asp61 and preservation of the intersubunit Asp61 Odelta2-Ndelta1 His87 hydrogen-bonded salt bridge in streptavidin-biotin versus apostreptavidin or streptavidin-peptide complexes is associated with an ordering of the flexible flap comprising residues Ala46 to Glu51, that in turn orders the Arg84 side-chain of a neighboring loop through resulting hydrogen bonds. Ordering of Arg84 in close proximity to the strong intersubunit interface appears to stabilize the conformation associated with the Asp61 Odelta2-Ndelta1 His87 hydrogen-bonded salt bridge. Thus, in addition to the established role of biotin in tetramer stabilization by direct mediation of intersubunit interactions at the weak interface through contact with Trp120, biotin may enhance tetramer stability at the strong interface more indirectly by ordering loop residues.
-
-	Binding of biotin to streptavidin stabilizes intersubunit salt bridges between Asp61 and His87 at low pH.,Katz BA J Mol Biol. 1997 Dec 19;274(5):776-800. PMID:9405158<ref>PMID:9405158</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2izl" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Avidin 3D structures\|Avidin 3D structures]]		*[[Avidin 3D structures\|Avidin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
-	[[Category: Katz~~, B A]]~~	+	[[Category: Katz BA]]
-	~~[[Category: Biotin-binding protein]]~~	+
-	~~[[Category: Streptavidin-2-iminobiotin~~]]	+

OCA at 10:42, 16 September 2020

OCA — Wed, 16 Sep 2020 10:42:09 GMT

←Older revision		Revision as of 10:42, 16 September 2020
Line 1:		Line 1:

	==STREPTAVIDIN-2-IMINOBIOTIN PH 7.3 I222 COMPLEX==		==STREPTAVIDIN-2-IMINOBIOTIN PH 7.3 I222 COMPLEX==
-	<StructureSection load='2izl' size='340' side='right' caption='[[2izl]], [[Resolution\|resolution]] 1.48Å' scene=''>	+	<StructureSection load='2izl' size='340' side='right'caption='[[2izl]], [[Resolution\|resolution]] 1.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2izl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2IZL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2izl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2IZL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMI:2-IMINOBIOTIN'>IMI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMI:2-IMINOBIOTIN'>IMI</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2izl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izl OCA], [http://pdbe.org/2izl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2izl RCSB], [http://www.ebi.ac.uk/pdbsum/2izl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2izl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2izl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izl OCA], [http://pdbe.org/2izl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2izl RCSB], [http://www.ebi.ac.uk/pdbsum/2izl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2izl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Avidin\|Avidin]]	+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: Katz, B A]]		[[Category: Katz, B A]]
	[[Category: Biotin-binding protein]]		[[Category: Biotin-binding protein]]
	[[Category: Streptavidin-2-iminobiotin]]		[[Category: Streptavidin-2-iminobiotin]]

OCA at 07:54, 12 December 2018

OCA — Wed, 12 Dec 2018 07:54:34 GMT

←Older revision		Revision as of 07:54, 12 December 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iz/2izl_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iz/2izl_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 07:17, 9 March 2017

OCA — Thu, 09 Mar 2017 07:17:53 GMT

←Older revision		Revision as of 07:17, 9 March 2017
Line 1:		Line 1:
		+
	==STREPTAVIDIN-2-IMINOBIOTIN PH 7.3 I222 COMPLEX==		==STREPTAVIDIN-2-IMINOBIOTIN PH 7.3 I222 COMPLEX==
	<StructureSection load='2izl' size='340' side='right' caption='[[2izl]], [[Resolution\|resolution]] 1.48Å' scene=''>		<StructureSection load='2izl' size='340' side='right' caption='[[2izl]], [[Resolution\|resolution]] 1.48Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2izl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IZL FirstGlance]. <br>		<table><tr><td colspan='2'>[[2izl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IZL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMI:2-IMINOBIOTIN'>IMI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMI:2-IMINOBIOTIN'>IMI</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2izl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izl OCA], [http://pdbe.org/2izl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2izl RCSB], [http://www.ebi.ac.uk/pdbsum/2izl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2izl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izl OCA], [http://pdbe.org/2izl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2izl RCSB], [http://www.ebi.ac.uk/pdbsum/2izl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2izl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 00:39, 8 February 2016

OCA — Mon, 08 Feb 2016 00:39:56 GMT

←Older revision		Revision as of 00:39, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2izl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:17, 10 September 2015

OCA — Thu, 10 Sep 2015 06:17:31 GMT

←Older revision		Revision as of 06:17, 10 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[2izl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IZL FirstGlance]. <br>		<table><tr><td colspan='2'>[[2izl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IZL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMI:2-IMINOBIOTIN'>IMI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMI:2-IMINOBIOTIN'>IMI</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2izl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2izl RCSB], [http://www.ebi.ac.uk/pdbsum/2izl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2izl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izl OCA], [http://pdbe.org/2izl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2izl RCSB], [http://www.ebi.ac.uk/pdbsum/2izl PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2izl" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 19:18, 24 December 2014

OCA — Wed, 24 Dec 2014 19:18:53 GMT

←Older revision		Revision as of 19:18, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2izl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IZL FirstGlance]. <br>		<table><tr><td colspan='2'>[[2izl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IZL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMI:2-IMINOBIOTIN'>IMI</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMI:2-IMINOBIOTIN'>IMI</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2izl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2izl RCSB], [http://www.ebi.ac.uk/pdbsum/2izl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2izl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2izl RCSB], [http://www.ebi.ac.uk/pdbsum/2izl PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
-	[[Category: Katz, B A.]]	+	[[Category: Katz, B A]]
	[[Category: Biotin-binding protein]]		[[Category: Biotin-binding protein]]
	[[Category: Streptavidin-2-iminobiotin]]		[[Category: Streptavidin-2-iminobiotin]]

OCA at 04:01, 29 September 2014

OCA — Mon, 29 Sep 2014 04:01:50 GMT

←Older revision		Revision as of 04:01, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2izl.~~png\|left~~\|200px]]	+	==STREPTAVIDIN-2-IMINOBIOTIN PH 7.3 I222 COMPLEX==
		+	<StructureSection load='2izl' size='340' side='right' caption='[[2izl]], [[Resolution\|resolution]] 1.48Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2izl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IZL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMI:2-IMINOBIOTIN'>IMI</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2izl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2izl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2izl RCSB], [http://www.ebi.ac.uk/pdbsum/2izl PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iz/2izl_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The remarkable stability of the streptavidin tetramer towards subunit dissociation becomes even greater upon binding of biotin. At two equivalent extensive monomer-monomer interfaces, monomers tightly associate into dimers that in turn associate into the tetramer at a less extensive dimer-dimer interface. To probe the structural basis for the enhancement of the stability of streptavidin by biotin, the crystal structures of apostreptavidin and its complexes with biotin and other small molecule and cyclic peptide ligands were determined and compared at resolutions as high as 1.36 A over a range of pH values from as low as 1.39. At low pH dramatic changes occur in the conformation and intersubunit hydrogen bonds involving the loop comprising Asp61 to Ser69. The hydrogen-bonded salt bridge between Asp61 Odelta2 and His87 Ndelta1, observed at higher pH, is replaced with a strong hydrogen bond between Asp61 Odelta1 and Asn85 Odelta1. Through crystallography at multiple pH values, the pH where this conformational change occurs, and thus the pKa of Asp61, was determined in crystals of space group I222 and/or I4122 of apostreptavidin and complexes. A range in pKa values for Asp61 was observed in these structures, the lowest being 1.78+/-0.19 for I222 streptavidin-biotin in 2.9 M (NH4)2SO4. At low pH the decrease in pKa of Asp61 and preservation of the intersubunit Asp61 Odelta2-Ndelta1 His87 hydrogen-bonded salt bridge in streptavidin-biotin versus apostreptavidin or streptavidin-peptide complexes is associated with an ordering of the flexible flap comprising residues Ala46 to Glu51, that in turn orders the Arg84 side-chain of a neighboring loop through resulting hydrogen bonds. Ordering of Arg84 in close proximity to the strong intersubunit interface appears to stabilize the conformation associated with the Asp61 Odelta2-Ndelta1 His87 hydrogen-bonded salt bridge. Thus, in addition to the established role of biotin in tetramer stabilization by direct mediation of intersubunit interactions at the weak interface through contact with Trp120, biotin may enhance tetramer stability at the strong interface more indirectly by ordering loop residues.

-	~~{{STRUCTURE_2izl\| PDB=2izl \| SCENE= }}~~	+	Binding of biotin to streptavidin stabilizes intersubunit salt bridges between Asp61 and His87 at low pH.,Katz BA J Mol Biol. 1997 Dec 19;274(5):776-800. PMID:9405158<ref>PMID:9405158</ref>

-	~~===STREPTAVIDIN-2-IMINOBIOTIN PH 7.3 I222 COMPLEX===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9405158}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2izl]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_avidinii Streptomyces avidinii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA].~~	+

	==See Also==		==See Also==
	*[[Avidin\|Avidin]]		*[[Avidin\|Avidin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:009405158<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: Katz, B A.]]		[[Category: Katz, B A.]]
	[[Category: Biotin-binding protein]]		[[Category: Biotin-binding protein]]
	[[Category: Streptavidin-2-iminobiotin]]		[[Category: Streptavidin-2-iminobiotin]]

OCA at 08:19, 20 October 2012

OCA — Sat, 20 Oct 2012 08:19:59 GMT

←Older revision		Revision as of 08:19, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2izl]] is a 2 chain structure ~~of [[Avidin]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA].	+	[[2izl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IZL OCA].

	==See Also==		==See Also==

OCA at 19:12, 25 July 2012

OCA — Wed, 25 Jul 2012 19:12:01 GMT

←Older revision		Revision as of 19:12, 25 July 2012
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	[[Image:2izl.png\|left\|200px]]		[[Image:2izl.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2izl\| PDB=2izl \| SCENE= }}		{{STRUCTURE_2izl\| PDB=2izl \| SCENE= }}

	===STREPTAVIDIN-2-IMINOBIOTIN PH 7.3 I222 COMPLEX===		===STREPTAVIDIN-2-IMINOBIOTIN PH 7.3 I222 COMPLEX===

-
-	<!--
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	{{ABSTRACT_PUBMED_9405158}}		{{ABSTRACT_PUBMED_9405158}}

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	==See Also==		==See Also==
-	*[[Avidin]]	+	*[[Avidin\|Avidin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9405158~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009405158</ref><references group="xtra"/>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: Katz, B A.]]		[[Category: Katz, B A.]]
	[[Category: Biotin-binding protein]]		[[Category: Biotin-binding protein]]
	[[Category: Streptavidin-2-iminobiotin]]		[[Category: Streptavidin-2-iminobiotin]]