2j1d - Revision history

OCA at 09:33, 9 May 2024

OCA — Thu, 09 May 2024 09:33:20 GMT

←Older revision		Revision as of 09:33, 9 May 2024
Line 3:		Line 3:
	<StructureSection load='2j1d' size='340' side='right'caption='[[2j1d]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2j1d' size='340' side='right'caption='[[2j1d]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J1D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J1D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.25Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [https://pdbe.org/2j1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [https://www.ebi.ac.uk/pdbsum/2j1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j1d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [https://pdbe.org/2j1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [https://www.ebi.ac.uk/pdbsum/2j1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j1d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DAAM1_HUMAN DAAM1_HUMAN]] Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments.<ref>PMID:16630611</ref> <ref>PMID:17482208</ref>	+	[https://www.uniprot.org/uniprot/DAAM1_HUMAN DAAM1_HUMAN] Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments.<ref>PMID:16630611</ref> <ref>PMID:17482208</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Eck~~, M J~~]]	+	[[Category: Eck MJ]]
-	[[Category: Lu, J]]	+	[[Category: Lu J]]
-	[[Category: Meng, W]]	+	[[Category: Meng W]]
-	[[Category: Poy, F]]	+	[[Category: Poy F]]
-	~~[[Category: Actin assembly]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 07:58, 19 January 2022

OCA — Wed, 19 Jan 2022 07:58:47 GMT

←Older revision		Revision as of 07:58, 19 January 2022
Line 3:		Line 3:
	<StructureSection load='2j1d' size='340' side='right'caption='[[2j1d]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2j1d' size='340' side='right'caption='[[2j1d]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2J1D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J1D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [~~http~~://pdbe.org/2j1d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2j1d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2j1d ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [https://pdbe.org/2j1d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [https://www.ebi.ac.uk/pdbsum/2j1d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j1d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DAAM1_HUMAN DAAM1_HUMAN]] Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments.<ref>PMID:16630611</ref> <ref>PMID:17482208</ref>	+	[[https://www.uniprot.org/uniprot/DAAM1_HUMAN DAAM1_HUMAN]] Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments.<ref>PMID:16630611</ref> <ref>PMID:17482208</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:36, 15 April 2020

OCA — Wed, 15 Apr 2020 07:36:35 GMT

←Older revision		Revision as of 07:36, 15 April 2020
Line 1:		Line 1:

	==Crystallization of hDaam1 C-terminal Fragment==		==Crystallization of hDaam1 C-terminal Fragment==
-	<StructureSection load='2j1d' size='340' side='right' caption='[[2j1d]], [[Resolution\|resolution]] 2.25Å' scene=''>	+	<StructureSection load='2j1d' size='340' side='right'caption='[[2j1d]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2J1D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2J1D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [http://pdbe.org/2j1d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [http://www.ebi.ac.uk/pdbsum/2j1d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j1d ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [http://pdbe.org/2j1d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [http://www.ebi.ac.uk/pdbsum/2j1d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j1d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Eck, M J]]		[[Category: Eck, M J]]
	[[Category: Lu, J]]		[[Category: Lu, J]]

OCA at 06:42, 11 July 2018

OCA — Wed, 11 Jul 2018 06:42:29 GMT

←Older revision		Revision as of 06:42, 11 July 2018
Line 1:		Line 1:
-	==~~CRYSTALLIZATION OF HDAAM1~~ C-~~TERMINAL FRAGMENT~~==	+
		+	==Crystallization of hDaam1 C-terminal Fragment==
	<StructureSection load='2j1d' size='340' side='right' caption='[[2j1d]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2j1d' size='340' side='right' caption='[[2j1d]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J1D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J1D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [http://pdbe.org/2j1d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [http://www.ebi.ac.uk/pdbsum/2j1d PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [http://pdbe.org/2j1d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [http://www.ebi.ac.uk/pdbsum/2j1d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j1d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j1/2j1d_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j1/2j1d_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:34, 7 February 2016

OCA — Sun, 07 Feb 2016 06:34:54 GMT

←Older revision		Revision as of 06:34, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j1d ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:47, 9 September 2015

OCA — Wed, 09 Sep 2015 22:47:11 GMT

←Older revision		Revision as of 22:47, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='2j1d' size='340' side='right' caption='[[2j1d]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2j1d' size='340' side='right' caption='[[2j1d]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J1D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J1D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [http://www.ebi.ac.uk/pdbsum/2j1d PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [http://pdbe.org/2j1d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [http://www.ebi.ac.uk/pdbsum/2j1d PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2j1d" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Eck, M J]]		[[Category: Eck, M J]]
	[[Category: Lu, J]]		[[Category: Lu, J]]

OCA at 08:40, 24 December 2014

OCA — Wed, 24 Dec 2014 08:40:28 GMT

←Older revision		Revision as of 08:40, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J1D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J1D FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [http://www.ebi.ac.uk/pdbsum/2j1d PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [http://www.ebi.ac.uk/pdbsum/2j1d PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DAAM1_HUMAN DAAM1_HUMAN]] Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments.<ref>PMID:16630611</ref> <ref>PMID:17482208</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Eck, M J.]]	+	[[Category: Eck, M J]]
-	[[Category: Lu, J.]]	+	[[Category: Lu, J]]
-	[[Category: Meng, W.]]	+	[[Category: Meng, W]]
-	[[Category: Poy, F.]]	+	[[Category: Poy, F]]
	[[Category: Actin assembly]]		[[Category: Actin assembly]]
	[[Category: Protein binding]]		[[Category: Protein binding]]

OCA at 09:23, 30 September 2014

OCA — Tue, 30 Sep 2014 09:23:00 GMT

←Older revision		Revision as of 09:23, 30 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2j1d.~~png\|left~~\|200px]]	+	==CRYSTALLIZATION OF HDAAM1 C-TERMINAL FRAGMENT==
		+	<StructureSection load='2j1d' size='340' side='right' caption='[[2j1d]], [[Resolution\|resolution]] 2.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2j1d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J1D FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j1d RCSB], [http://www.ebi.ac.uk/pdbsum/2j1d PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j1/2j1d_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Daam1 (dishevelled-associated activator of morphogenesis-1) is a diaphanous-related formin first studied as a novel dishevelled binding protein and shown to be crucial for the planar cell polarity (PCP) pathway in Xenopus. Daam1, like other formins, directs nucleation and elongation of new actin filaments using its conserved formin-homology-2 (FH2) domain. Here we report the crystal structure of a large C-terminal fragment of human Daam1 containing the FH2 domain. The structure, determined at 2.25 A resolution using the single-wavelength anomalous diffraction (SAD) phasing method, reveals a "tethered dimer" architecture that is similar to that previously described for the FH2 domain of the yeast formin Bni1, which shares approximately 21% sequence identity with Daam1. Despite the overall similarity with the dimeric FH2 domain of Bni1 and with a truncated monomeric structure of mDia1, the Daam1 FH2 structure reveals a number of differences in secondary structure elements and in the "lasso/post" dimerization interface that may be functionally important. Most strikingly, the two halves of the crystallographic dimer pack together in a manner that occludes their actin binding surfaces. This "locked" conformation is stabilized by two novel, interacting beta-strands formed by the ends of the linkers that connect the two sides of the dimer. The Daam1 FH2 domain has weak actin assembly activity as compared with other mammalian formins, but mutations that disrupt the beta-strand lock increase activity about tenfold to a level comparable to other formins, suggesting that this occluded conformation may represent an auto-inhibited conformation of the Daam1 FH2 domain.

-	~~{{STRUCTURE_2j1d\| PDB=2j1d \| SCENE= }}~~	+	Structure of the FH2 domain of Daam1: implications for formin regulation of actin assembly.,Lu J, Meng W, Poy F, Maiti S, Goode BL, Eck MJ J Mol Biol. 2007 Jun 22;369(5):1258-69. Epub 2007 Apr 5. PMID:17482208<ref>PMID:17482208</ref>

-	~~===CRYSTALLIZATION OF HDAAM1 C-TERMINAL FRAGMENT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17482208}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2j1d]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017482208<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Eck, M J.]]		[[Category: Eck, M J.]]

OCA: Protected "2j1d" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 11:39:26 GMT

Protected "2j1d" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:39, 7 January 2013

OCA at 11:39, 7 January 2013

OCA — Mon, 07 Jan 2013 11:39:22 GMT

←Older revision		Revision as of 11:39, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2j1d.png\|left\|200px]]		[[Image:2j1d.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2j1d", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2j1d\| PDB=2j1d \| SCENE= }}		{{STRUCTURE_2j1d\| PDB=2j1d \| SCENE= }}

	===CRYSTALLIZATION OF HDAAM1 C-TERMINAL FRAGMENT===		===CRYSTALLIZATION OF HDAAM1 C-TERMINAL FRAGMENT===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17482208}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17482208 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17482208}}		{{ABSTRACT_PUBMED_17482208}}

	==About this Structure==		==About this Structure==
-	~~2J1D~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA].	+	[[2j1d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1D OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17482208~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017482208</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Eck, M J.]]		[[Category: Eck, M J.]]
Line 30:		Line 18:
	[[Category: Poy, F.]]		[[Category: Poy, F.]]
	[[Category: Actin assembly]]		[[Category: Actin assembly]]
-	[[Category: Alternative splicing]]
-	[[Category: Coiled coil]]
-	[[Category: Fh2 domain]]
-	[[Category: Formin]]
	[[Category: Protein binding]]		[[Category: Protein binding]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 22 10:52:35 2009''