2j7k - Revision history

OCA at 14:37, 13 December 2023

OCA — Wed, 13 Dec 2023 14:37:22 GMT

←Older revision		Revision as of 14:37, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='2j7k' size='340' side='right'caption='[[2j7k]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='2j7k' size='340' side='right'caption='[[2j7k]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j7k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Thet8 Thet8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J7K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j7k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J7K FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1fnm\|1fnm]], [[1ip8\|1ip8]], [[1ipm\|1ipm]], [[1ipo\|1ipo]], [[1ipr\|1ipr]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1ktv\|1ktv]], [[1pn6\|1pn6]], [[2bcw\|2bcw]], [[2bm0\|2bm0]], [[2bm1\|2bm1]], [[2bv3\|2bv3]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [https://pdbe.org/2j7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [https://www.ebi.ac.uk/pdbsum/2j7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j7k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [https://pdbe.org/2j7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [https://www.ebi.ac.uk/pdbsum/2j7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j7k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/EFG_THETH EFG_THETH]] Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.	+	[https://www.uniprot.org/uniprot/EFG_THETH EFG_THETH] Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j7k ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j7k ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Elongation factor G (EF-G) is a G protein factor that catalyzes the translocation step in protein synthesis on the ribosome. Its GTP conformation in the absence of the ribosome is currently unknown. We present the structure of a mutant EF-G (T84A) in complex with the non-hydrolysable GTP analogue GDPNP. The crystal structure provides a first insight into conformational changes induced in EF-G by GTP. Comparison of this structure with that of EF-G in complex with GDP suggests that the GTP and GDP conformations in solution are very similar and that the major contribution to the active GTPase conformation, which is quite different, therefore comes from its interaction with the ribosome.
-
-	Crystal structure of a mutant elongation factor G trapped with a GTP analogue.,Hansson S, Singh R, Gudkov AT, Liljas A, Logan DT FEBS Lett. 2005 Aug 15;579(20):4492-7. PMID:16083884<ref>PMID:16083884</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2j7k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Elongation factor 3D structures\|Elongation factor 3D structures]]		*[[Elongation factor 3D structures\|Elongation factor 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Protein-synthesizing GTPase]]~~	+	[[Category: Thermus thermophilus HB8]]
-	~~[[Category: Thet8~~]]	+	[[Category: Hansson S]]
-	[[Category: Hansson, S]]	+	[[Category: Logan DT]]
-	[[Category: Logan~~, D T]]~~	+
-	~~[[Category: Elongation factor]]~~	+
-	~~[[Category: Gtp-binding]]~~	+
-	~~[[Category: Mutation]]~~	+
-	~~[[Category: Nucleotide- binding]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: P-loop]]~~	+
-	~~[[Category: Protein biosynthesis]]~~	+
-	~~[[Category: Thr84ala]]~~	+
-	~~[[Category: Translation~~]]	+

OCA at 08:03, 19 January 2022

OCA — Wed, 19 Jan 2022 08:03:31 GMT

←Older revision		Revision as of 08:03, 19 January 2022
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	<StructureSection load='2j7k' size='340' side='right'caption='[[2j7k]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='2j7k' size='340' side='right'caption='[[2j7k]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j7k]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2J7K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j7k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J7K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fnm\|1fnm]], [[1ip8\|1ip8]], [[1ipm\|1ipm]], [[1ipo\|1ipo]], [[1ipr\|1ipr]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1ktv\|1ktv]], [[1pn6\|1pn6]], [[2bcw\|2bcw]], [[2bm0\|2bm0]], [[2bm1\|2bm1]], [[2bv3\|2bv3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1fnm\|1fnm]], [[1ip8\|1ip8]], [[1ipm\|1ipm]], [[1ipo\|1ipo]], [[1ipr\|1ipr]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1ktv\|1ktv]], [[1pn6\|1pn6]], [[2bcw\|2bcw]], [[2bm0\|2bm0]], [[2bm1\|2bm1]], [[2bv3\|2bv3]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [~~http~~://pdbe.org/2j7k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2j7k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2j7k ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [https://pdbe.org/2j7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [https://www.ebi.ac.uk/pdbsum/2j7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j7k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/EFG_THETH EFG_THETH]] Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.	+	[[https://www.uniprot.org/uniprot/EFG_THETH EFG_THETH]] Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:42, 15 April 2020

OCA — Wed, 15 Apr 2020 07:42:52 GMT

←Older revision		Revision as of 07:42, 15 April 2020
Line 1:		Line 1:

	==Crystal structure of the T84A mutant EF-G:GDPCP complex==		==Crystal structure of the T84A mutant EF-G:GDPCP complex==
-	<StructureSection load='2j7k' size='340' side='right' caption='[[2j7k]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='2j7k' size='340' side='right'caption='[[2j7k]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j7k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2J7K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j7k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2J7K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fnm\|1fnm]], [[1ip8\|1ip8]], [[1ipm\|1ipm]], [[1ipo\|1ipo]], [[1ipr\|1ipr]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1ktv\|1ktv]], [[1pn6\|1pn6]], [[2bcw\|2bcw]], [[2bm0\|2bm0]], [[2bm1\|2bm1]], [[2bv3\|2bv3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fnm\|1fnm]], [[1ip8\|1ip8]], [[1ipm\|1ipm]], [[1ipo\|1ipo]], [[1ipr\|1ipr]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1ktv\|1ktv]], [[1pn6\|1pn6]], [[2bcw\|2bcw]], [[2bm0\|2bm0]], [[2bm1\|2bm1]], [[2bv3\|2bv3]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [http://pdbe.org/2j7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [http://www.ebi.ac.uk/pdbsum/2j7k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j7k ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [http://pdbe.org/2j7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [http://www.ebi.ac.uk/pdbsum/2j7k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j7k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Elongation factor\|Elongation factor]]	+	*[[Elongation factor 3D structures\|Elongation factor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Protein-synthesizing GTPase]]		[[Category: Protein-synthesizing GTPase]]
	[[Category: Thet8]]		[[Category: Thet8]]

OCA at 06:32, 11 July 2018

OCA — Wed, 11 Jul 2018 06:32:21 GMT

←Older revision		Revision as of 06:32, 11 July 2018
Line 1:		Line 1:
-	==~~CRYSTAL STRUCTURE OF THE~~ T84A ~~MUTANT~~ EF-G:GDPCP ~~COMPLEX~~==	+
		+	==Crystal structure of the T84A mutant EF-G:GDPCP complex==
	<StructureSection load='2j7k' size='340' side='right' caption='[[2j7k]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='2j7k' size='340' side='right' caption='[[2j7k]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fnm\|1fnm]], [[1ip8\|1ip8]], [[1ipm\|1ipm]], [[1ipo\|1ipo]], [[1ipr\|1ipr]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1ktv\|1ktv]], [[1pn6\|1pn6]], [[2bcw\|2bcw]], [[2bm0\|2bm0]], [[2bm1\|2bm1]], [[2bv3\|2bv3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fnm\|1fnm]], [[1ip8\|1ip8]], [[1ipm\|1ipm]], [[1ipo\|1ipo]], [[1ipr\|1ipr]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1ktv\|1ktv]], [[1pn6\|1pn6]], [[2bcw\|2bcw]], [[2bm0\|2bm0]], [[2bm1\|2bm1]], [[2bv3\|2bv3]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [http://pdbe.org/2j7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [http://www.ebi.ac.uk/pdbsum/2j7k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [http://pdbe.org/2j7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [http://www.ebi.ac.uk/pdbsum/2j7k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j7k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j7/2j7k_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j7/2j7k_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 05:37, 9 February 2016

OCA — Tue, 09 Feb 2016 05:37:19 GMT

←Older revision		Revision as of 05:37, 9 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j7k ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:08, 10 September 2015

OCA — Thu, 10 Sep 2015 19:08:54 GMT

←Older revision		Revision as of 19:08, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2j7k' size='340' side='right' caption='[[2j7k]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='2j7k' size='340' side='right' caption='[[2j7k]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j7k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus Thermus thermophilus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J7K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j7k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J7K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fnm\|1fnm]], [[1ip8\|1ip8]], [[1ipm\|1ipm]], [[1ipo\|1ipo]], [[1ipr\|1ipr]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1ktv\|1ktv]], [[1pn6\|1pn6]], [[2bcw\|2bcw]], [[2bm0\|2bm0]], [[2bm1\|2bm1]], [[2bv3\|2bv3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fnm\|1fnm]], [[1ip8\|1ip8]], [[1ipm\|1ipm]], [[1ipo\|1ipo]], [[1ipr\|1ipr]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1ktv\|1ktv]], [[1pn6\|1pn6]], [[2bcw\|2bcw]], [[2bm0\|2bm0]], [[2bm1\|2bm1]], [[2bv3\|2bv3]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [http://www.ebi.ac.uk/pdbsum/2j7k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [http://pdbe.org/2j7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [http://www.ebi.ac.uk/pdbsum/2j7k PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2j7k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Protein-synthesizing GTPase]]		[[Category: Protein-synthesizing GTPase]]
-	[[Category: ~~Thermus thermophilus~~]]	+	[[Category: Thet8]]
	[[Category: Hansson, S]]		[[Category: Hansson, S]]
	[[Category: Logan, D T]]		[[Category: Logan, D T]]

OCA at 04:47, 25 December 2014

OCA — Thu, 25 Dec 2014 04:47:07 GMT

←Older revision		Revision as of 04:47, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2j7k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J7K FirstGlance]. <br>		<table><tr><td colspan='2'>[[2j7k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J7K FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fnm\|1fnm]], [[1ip8\|1ip8]], [[1ipm\|1ipm]], [[1ipo\|1ipo]], [[1ipr\|1ipr]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1ktv\|1ktv]], [[1pn6\|1pn6]], [[2bcw\|2bcw]], [[2bm0\|2bm0]], [[2bm1\|2bm1]], [[2bv3\|2bv3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fnm\|1fnm]], [[1ip8\|1ip8]], [[1ipm\|1ipm]], [[1ipo\|1ipo]], [[1ipr\|1ipr]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1ktv\|1ktv]], [[1pn6\|1pn6]], [[2bcw\|2bcw]], [[2bm0\|2bm0]], [[2bm1\|2bm1]], [[2bv3\|2bv3]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [http://www.ebi.ac.uk/pdbsum/2j7k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [http://www.ebi.ac.uk/pdbsum/2j7k PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/EFG_THETH EFG_THETH]] Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Protein-synthesizing GTPase]]		[[Category: Protein-synthesizing GTPase]]
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
-	[[Category: Hansson, S.]]	+	[[Category: Hansson, S]]
-	[[Category: Logan, D T.]]	+	[[Category: Logan, D T]]
	[[Category: Elongation factor]]		[[Category: Elongation factor]]
	[[Category: Gtp-binding]]		[[Category: Gtp-binding]]

OCA at 04:28, 29 September 2014

OCA — Mon, 29 Sep 2014 04:28:00 GMT

←Older revision		Revision as of 04:28, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2j7k.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE T84A MUTANT EF-G:GDPCP COMPLEX==
		+	<StructureSection load='2j7k' size='340' side='right' caption='[[2j7k]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2j7k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J7K FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fnm\|1fnm]], [[1ip8\|1ip8]], [[1ipm\|1ipm]], [[1ipo\|1ipo]], [[1ipr\|1ipr]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1ktv\|1ktv]], [[1pn6\|1pn6]], [[2bcw\|2bcw]], [[2bm0\|2bm0]], [[2bm1\|2bm1]], [[2bv3\|2bv3]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j7k RCSB], [http://www.ebi.ac.uk/pdbsum/2j7k PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j7/2j7k_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Elongation factor G (EF-G) is a G protein factor that catalyzes the translocation step in protein synthesis on the ribosome. Its GTP conformation in the absence of the ribosome is currently unknown. We present the structure of a mutant EF-G (T84A) in complex with the non-hydrolysable GTP analogue GDPNP. The crystal structure provides a first insight into conformational changes induced in EF-G by GTP. Comparison of this structure with that of EF-G in complex with GDP suggests that the GTP and GDP conformations in solution are very similar and that the major contribution to the active GTPase conformation, which is quite different, therefore comes from its interaction with the ribosome.

-	~~{{STRUCTURE_2j7k\| PDB=2j7k \| SCENE= }}~~	+	Crystal structure of a mutant elongation factor G trapped with a GTP analogue.,Hansson S, Singh R, Gudkov AT, Liljas A, Logan DT FEBS Lett. 2005 Aug 15;579(20):4492-7. PMID:16083884<ref>PMID:16083884</ref>

-	~~===CRYSTAL STRUCTURE OF THE T84A MUTANT EF-G:GDPCP COMPLEX===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16083884}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2j7k]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermus_thermophilus Thermus thermophilus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA].~~	+

	==See Also==		==See Also==
	*[[Elongation factor\|Elongation factor]]		*[[Elongation factor\|Elongation factor]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016083884<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Protein-synthesizing GTPase]]		[[Category: Protein-synthesizing GTPase]]
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]

OCA at 12:49, 20 October 2012

OCA — Sat, 20 Oct 2012 12:49:21 GMT

←Older revision		Revision as of 12:49, 20 October 2012
Line 1:		Line 1:
	[[Image:2j7k.png\|left\|200px]]		[[Image:2j7k.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2j7k", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2j7k\| PDB=2j7k \| SCENE= }}		{{STRUCTURE_2j7k\| PDB=2j7k \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE T84A MUTANT EF-G:GDPCP COMPLEX===		===CRYSTAL STRUCTURE OF THE T84A MUTANT EF-G:GDPCP COMPLEX===

		+	{{ABSTRACT_PUBMED_16083884}}

	==About this Structure==		==About this Structure==
	[[2j7k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA].		[[2j7k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7K OCA].
		+
		+	==See Also==
		+	*[[Elongation factor\|Elongation factor]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16083884~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016083884</ref><references group="xtra"/>
	[[Category: Protein-synthesizing GTPase]]		[[Category: Protein-synthesizing GTPase]]
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]

OCA: Protected "2j7k" [edit=sysop:move=sysop]

OCA — Mon, 14 Mar 2011 12:49:20 GMT

Protected "2j7k" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:49, 14 March 2011