2j9h - Revision history

OCA at 14:39, 13 December 2023

OCA — Wed, 13 Dec 2023 14:39:45 GMT

←Older revision		Revision as of 14:39, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='2j9h' size='340' side='right'caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2j9h' size='340' side='right'caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j9h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J9H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j9h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J9H FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GTX:S~~-~~HEXYLGLUTATHIONE'>GTX</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[10gs\|10gs]], [[11gs\|11gs]], [[12gs\|12gs]], [[13gs\|13gs]], [[14gs\|14gs]], [[16gs\|16gs]], [[17gs\|17gs]], [[18gs\|18gs]], [[19gs\|19gs]], [[1aqv\|1aqv]], [[1aqw\|1aqw]], [[1aqx\|1aqx]], [[1eog\|1eog]], [[1eoh\|1eoh]], [[1gss\|1gss]], [[1kbn\|1kbn]], [[1lbk\|1lbk]], [[1md3\|1md3]], [[1md4\|1md4]], [[1pgt\|1pgt]], [[1px6\|1px6]], [[1px7\|1px7]], [[1zgn\|1zgn]], [[20gs\|20gs]], [[21gs\|21gs]], [[22gs\|22gs]], [[2a2r\|2a2r]], [[2a2s\|2a2s]], [[2gss\|2gss]], [[2pgt\|2pgt]], [[3gss\|3gss]], [[3pgt\|3pgt]], [[4gss\|4gss]], [[4pgt\|4pgt]], [[5gss\|5gss]], [[6gss\|6gss]], [[7gss\|7gss]], [[8gss\|8gss]], [[9gss\|9gss]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9h OCA], [https://pdbe.org/2j9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j9h RCSB], [https://www.ebi.ac.uk/pdbsum/2j9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j9h ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9h OCA], [https://pdbe.org/2j9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j9h RCSB], [https://www.ebi.ac.uk/pdbsum/2j9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j9h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GSTP1_HUMAN GSTP1_HUMAN]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.<ref>PMID:21668448</ref>	+	[https://www.uniprot.org/uniprot/GSTP1_HUMAN GSTP1_HUMAN] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.<ref>PMID:21668448</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Glutathione transferase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hegazy~~, U M~~]]	+	[[Category: Hegazy UM]]
-	[[Category: Hellman, U]]	+	[[Category: Hellman U]]
-	[[Category: Mannervik, B]]	+	[[Category: Mannervik B]]
-	[[Category: Tars, K]]	+	[[Category: Tars K]]
-	~~[[Category: Glutathione]]~~	+
-	~~[[Category: P1-1]]~~	+
-	~~[[Category: Polymorphism]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 11:25, 31 March 2021

OCA — Wed, 31 Mar 2021 11:25:34 GMT

←Older revision		Revision as of 11:25, 31 March 2021
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	<StructureSection load='2j9h' size='340' side='right'caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2j9h' size='340' side='right'caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j9h]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9H OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2J9H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j9h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J9H FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[10gs\|10gs]], [[11gs\|11gs]], [[12gs\|12gs]], [[13gs\|13gs]], [[14gs\|14gs]], [[16gs\|16gs]], [[17gs\|17gs]], [[18gs\|18gs]], [[19gs\|19gs]], [[1aqv\|1aqv]], [[1aqw\|1aqw]], [[1aqx\|1aqx]], [[1eog\|1eog]], [[1eoh\|1eoh]], [[1gss\|1gss]], [[1kbn\|1kbn]], [[1lbk\|1lbk]], [[1md3\|1md3]], [[1md4\|1md4]], [[1pgt\|1pgt]], [[1px6\|1px6]], [[1px7\|1px7]], [[1zgn\|1zgn]], [[20gs\|20gs]], [[21gs\|21gs]], [[22gs\|22gs]], [[2a2r\|2a2r]], [[2a2s\|2a2s]], [[2gss\|2gss]], [[2pgt\|2pgt]], [[3gss\|3gss]], [[3pgt\|3pgt]], [[4gss\|4gss]], [[4pgt\|4pgt]], [[5gss\|5gss]], [[6gss\|6gss]], [[7gss\|7gss]], [[8gss\|8gss]], [[9gss\|9gss]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[10gs\|10gs]], [[11gs\|11gs]], [[12gs\|12gs]], [[13gs\|13gs]], [[14gs\|14gs]], [[16gs\|16gs]], [[17gs\|17gs]], [[18gs\|18gs]], [[19gs\|19gs]], [[1aqv\|1aqv]], [[1aqw\|1aqw]], [[1aqx\|1aqx]], [[1eog\|1eog]], [[1eoh\|1eoh]], [[1gss\|1gss]], [[1kbn\|1kbn]], [[1lbk\|1lbk]], [[1md3\|1md3]], [[1md4\|1md4]], [[1pgt\|1pgt]], [[1px6\|1px6]], [[1px7\|1px7]], [[1zgn\|1zgn]], [[20gs\|20gs]], [[21gs\|21gs]], [[22gs\|22gs]], [[2a2r\|2a2r]], [[2a2s\|2a2s]], [[2gss\|2gss]], [[2pgt\|2pgt]], [[3gss\|3gss]], [[3pgt\|3pgt]], [[4gss\|4gss]], [[4pgt\|4pgt]], [[5gss\|5gss]], [[6gss\|6gss]], [[7gss\|7gss]], [[8gss\|8gss]], [[9gss\|9gss]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2j9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9h OCA], [~~http~~://pdbe.org/2j9h PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2j9h RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2j9h PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2j9h ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9h OCA], [https://pdbe.org/2j9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j9h RCSB], [https://www.ebi.ac.uk/pdbsum/2j9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j9h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GSTP1_HUMAN GSTP1_HUMAN]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.<ref>PMID:21668448</ref>	+	[[https://www.uniprot.org/uniprot/GSTP1_HUMAN GSTP1_HUMAN]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.<ref>PMID:21668448</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
-	*[[Glutathione S-transferase\|Glutathione S-transferase]]	+	*[[Glutathione S-transferase 3D structures\|Glutathione S-transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:05, 10 May 2019

OCA — Fri, 10 May 2019 12:05:20 GMT

←Older revision		Revision as of 12:05, 10 May 2019
Line 1:		Line 1:

	==Crystal structure of human glutathione-S-transferase P1-1 cys-free mutant in complex with S-hexylglutathione at 2.4 A resolution==		==Crystal structure of human glutathione-S-transferase P1-1 cys-free mutant in complex with S-hexylglutathione at 2.4 A resolution==
-	<StructureSection load='2j9h' size='340' side='right' caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='2j9h' size='340' side='right'caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2j9h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J9H FirstGlance]. <br>		<table><tr><td colspan='2'>[[2j9h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J9H FirstGlance]. <br>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 2j9h" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2j9h" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutathione S-transferase\|Glutathione S-transferase]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Hegazy, U M]]		[[Category: Hegazy, U M]]
	[[Category: Hellman, U]]		[[Category: Hellman, U]]

OCA at 07:22, 17 January 2018

OCA — Wed, 17 Jan 2018 07:22:40 GMT

←Older revision		Revision as of 07:22, 17 January 2018
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j9/2j9h_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j9/2j9h_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 15:55, 16 November 2017

OCA — Thu, 16 Nov 2017 15:55:57 GMT

←Older revision		Revision as of 15:55, 16 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of human glutathione-S-transferase P1-1 cys-free mutant in complex with S-hexylglutathione at 2.4 A resolution==		==Crystal structure of human glutathione-S-transferase P1-1 cys-free mutant in complex with S-hexylglutathione at 2.4 A resolution==
	<StructureSection load='2j9h' size='340' side='right' caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2j9h' size='340' side='right' caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[10gs\|10gs]], [[11gs\|11gs]], [[12gs\|12gs]], [[13gs\|13gs]], [[14gs\|14gs]], [[16gs\|16gs]], [[17gs\|17gs]], [[18gs\|18gs]], [[19gs\|19gs]], [[1aqv\|1aqv]], [[1aqw\|1aqw]], [[1aqx\|1aqx]], [[1eog\|1eog]], [[1eoh\|1eoh]], [[1gss\|1gss]], [[1kbn\|1kbn]], [[1lbk\|1lbk]], [[1md3\|1md3]], [[1md4\|1md4]], [[1pgt\|1pgt]], [[1px6\|1px6]], [[1px7\|1px7]], [[1zgn\|1zgn]], [[20gs\|20gs]], [[21gs\|21gs]], [[22gs\|22gs]], [[2a2r\|2a2r]], [[2a2s\|2a2s]], [[2gss\|2gss]], [[2pgt\|2pgt]], [[3gss\|3gss]], [[3pgt\|3pgt]], [[4gss\|4gss]], [[4pgt\|4pgt]], [[5gss\|5gss]], [[6gss\|6gss]], [[7gss\|7gss]], [[8gss\|8gss]], [[9gss\|9gss]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[10gs\|10gs]], [[11gs\|11gs]], [[12gs\|12gs]], [[13gs\|13gs]], [[14gs\|14gs]], [[16gs\|16gs]], [[17gs\|17gs]], [[18gs\|18gs]], [[19gs\|19gs]], [[1aqv\|1aqv]], [[1aqw\|1aqw]], [[1aqx\|1aqx]], [[1eog\|1eog]], [[1eoh\|1eoh]], [[1gss\|1gss]], [[1kbn\|1kbn]], [[1lbk\|1lbk]], [[1md3\|1md3]], [[1md4\|1md4]], [[1pgt\|1pgt]], [[1px6\|1px6]], [[1px7\|1px7]], [[1zgn\|1zgn]], [[20gs\|20gs]], [[21gs\|21gs]], [[22gs\|22gs]], [[2a2r\|2a2r]], [[2a2s\|2a2s]], [[2gss\|2gss]], [[2pgt\|2pgt]], [[3gss\|3gss]], [[3pgt\|3pgt]], [[4gss\|4gss]], [[4pgt\|4pgt]], [[5gss\|5gss]], [[6gss\|6gss]], [[7gss\|7gss]], [[8gss\|8gss]], [[9gss\|9gss]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9h OCA], [http://pdbe.org/2j9h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j9h RCSB], [http://www.ebi.ac.uk/pdbsum/2j9h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9h OCA], [http://pdbe.org/2j9h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j9h RCSB], [http://www.ebi.ac.uk/pdbsum/2j9h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j9h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 2j9h" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2j9h" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Glutathione S-transferase\|Glutathione S-transferase]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Glutathione]]		[[Category: Glutathione]]
	[[Category: P1-1]]		[[Category: P1-1]]
		+	[[Category: Polymorphism]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 14:31, 8 February 2016

OCA — Mon, 08 Feb 2016 14:31:10 GMT

←Older revision		Revision as of 14:31, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j9h ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:34, 30 September 2015

OCA — Wed, 30 Sep 2015 06:34:50 GMT

←Older revision		Revision as of 06:34, 30 September 2015
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-	==~~CRYSTAL STRUCTURE OF HUMAN GLUTATHIONE~~-S-~~TRANSFERASE~~ P1-1 ~~CYS~~-~~FREE MUTANT IN COMPLEX WITH~~ S-~~HEXYLGLUTATHIONE AT~~ 2.4 A ~~RESOLUTION~~==	+	==Crystal structure of human glutathione-S-transferase P1-1 cys-free mutant in complex with S-hexylglutathione at 2.4 A resolution==
	<StructureSection load='2j9h' size='340' side='right' caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2j9h' size='340' side='right' caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 12:30, 10 September 2015

OCA — Thu, 10 Sep 2015 12:30:08 GMT

←Older revision		Revision as of 12:30, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2j9h' size='340' side='right' caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2j9h' size='340' side='right' caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2j9h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J9H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2j9h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J9H FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[10gs\|10gs]], [[11gs\|11gs]], [[12gs\|12gs]], [[13gs\|13gs]], [[14gs\|14gs]], [[16gs\|16gs]], [[17gs\|17gs]], [[18gs\|18gs]], [[19gs\|19gs]], [[1aqv\|1aqv]], [[1aqw\|1aqw]], [[1aqx\|1aqx]], [[1eog\|1eog]], [[1eoh\|1eoh]], [[1gss\|1gss]], [[1kbn\|1kbn]], [[1lbk\|1lbk]], [[1md3\|1md3]], [[1md4\|1md4]], [[1pgt\|1pgt]], [[1px6\|1px6]], [[1px7\|1px7]], [[1zgn\|1zgn]], [[20gs\|20gs]], [[21gs\|21gs]], [[22gs\|22gs]], [[2a2r\|2a2r]], [[2a2s\|2a2s]], [[2gss\|2gss]], [[2pgt\|2pgt]], [[3gss\|3gss]], [[3pgt\|3pgt]], [[4gss\|4gss]], [[4pgt\|4pgt]], [[5gss\|5gss]], [[6gss\|6gss]], [[7gss\|7gss]], [[8gss\|8gss]], [[9gss\|9gss]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[10gs\|10gs]], [[11gs\|11gs]], [[12gs\|12gs]], [[13gs\|13gs]], [[14gs\|14gs]], [[16gs\|16gs]], [[17gs\|17gs]], [[18gs\|18gs]], [[19gs\|19gs]], [[1aqv\|1aqv]], [[1aqw\|1aqw]], [[1aqx\|1aqx]], [[1eog\|1eog]], [[1eoh\|1eoh]], [[1gss\|1gss]], [[1kbn\|1kbn]], [[1lbk\|1lbk]], [[1md3\|1md3]], [[1md4\|1md4]], [[1pgt\|1pgt]], [[1px6\|1px6]], [[1px7\|1px7]], [[1zgn\|1zgn]], [[20gs\|20gs]], [[21gs\|21gs]], [[22gs\|22gs]], [[2a2r\|2a2r]], [[2a2s\|2a2s]], [[2gss\|2gss]], [[2pgt\|2pgt]], [[3gss\|3gss]], [[3pgt\|3pgt]], [[4gss\|4gss]], [[4pgt\|4pgt]], [[5gss\|5gss]], [[6gss\|6gss]], [[7gss\|7gss]], [[8gss\|8gss]], [[9gss\|9gss]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j9h RCSB], [http://www.ebi.ac.uk/pdbsum/2j9h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9h OCA], [http://pdbe.org/2j9h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j9h RCSB], [http://www.ebi.ac.uk/pdbsum/2j9h PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2j9h" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Hegazy, U M]]		[[Category: Hegazy, U M]]
	[[Category: Hellman, U]]		[[Category: Hellman, U]]

OCA at 00:01, 25 December 2014

OCA — Thu, 25 Dec 2014 00:01:34 GMT

←Older revision		Revision as of 00:01, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2j9h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J9H FirstGlance]. <br>		<table><tr><td colspan='2'>[[2j9h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J9H FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[10gs\|10gs]], [[11gs\|11gs]], [[12gs\|12gs]], [[13gs\|13gs]], [[14gs\|14gs]], [[16gs\|16gs]], [[17gs\|17gs]], [[18gs\|18gs]], [[19gs\|19gs]], [[1aqv\|1aqv]], [[1aqw\|1aqw]], [[1aqx\|1aqx]], [[1eog\|1eog]], [[1eoh\|1eoh]], [[1gss\|1gss]], [[1kbn\|1kbn]], [[1lbk\|1lbk]], [[1md3\|1md3]], [[1md4\|1md4]], [[1pgt\|1pgt]], [[1px6\|1px6]], [[1px7\|1px7]], [[1zgn\|1zgn]], [[20gs\|20gs]], [[21gs\|21gs]], [[22gs\|22gs]], [[2a2r\|2a2r]], [[2a2s\|2a2s]], [[2gss\|2gss]], [[2pgt\|2pgt]], [[3gss\|3gss]], [[3pgt\|3pgt]], [[4gss\|4gss]], [[4pgt\|4pgt]], [[5gss\|5gss]], [[6gss\|6gss]], [[7gss\|7gss]], [[8gss\|8gss]], [[9gss\|9gss]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[10gs\|10gs]], [[11gs\|11gs]], [[12gs\|12gs]], [[13gs\|13gs]], [[14gs\|14gs]], [[16gs\|16gs]], [[17gs\|17gs]], [[18gs\|18gs]], [[19gs\|19gs]], [[1aqv\|1aqv]], [[1aqw\|1aqw]], [[1aqx\|1aqx]], [[1eog\|1eog]], [[1eoh\|1eoh]], [[1gss\|1gss]], [[1kbn\|1kbn]], [[1lbk\|1lbk]], [[1md3\|1md3]], [[1md4\|1md4]], [[1pgt\|1pgt]], [[1px6\|1px6]], [[1px7\|1px7]], [[1zgn\|1zgn]], [[20gs\|20gs]], [[21gs\|21gs]], [[22gs\|22gs]], [[2a2r\|2a2r]], [[2a2s\|2a2s]], [[2gss\|2gss]], [[2pgt\|2pgt]], [[3gss\|3gss]], [[3pgt\|3pgt]], [[4gss\|4gss]], [[4pgt\|4pgt]], [[5gss\|5gss]], [[6gss\|6gss]], [[7gss\|7gss]], [[8gss\|8gss]], [[9gss\|9gss]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j9h RCSB], [http://www.ebi.ac.uk/pdbsum/2j9h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j9h RCSB], [http://www.ebi.ac.uk/pdbsum/2j9h PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GSTP1_HUMAN GSTP1_HUMAN]] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.<ref>PMID:21668448</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Hegazy, U M.]]	+	[[Category: Hegazy, U M]]
-	[[Category: Hellman, U.]]	+	[[Category: Hellman, U]]
-	[[Category: Mannervik, B.]]	+	[[Category: Mannervik, B]]
-	[[Category: Tars, K.]]	+	[[Category: Tars, K]]
	[[Category: Glutathione]]		[[Category: Glutathione]]
-	[[Category: Glutathione transferase]]
	[[Category: P1-1]]		[[Category: P1-1]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 10:28, 30 September 2014

OCA — Tue, 30 Sep 2014 10:28:40 GMT

←Older revision		Revision as of 10:28, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2j9h.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF HUMAN GLUTATHIONE-S-TRANSFERASE P1-1 CYS-FREE MUTANT IN COMPLEX WITH S-HEXYLGLUTATHIONE AT 2.4 A RESOLUTION==
		+	<StructureSection load='2j9h' size='340' side='right' caption='[[2j9h]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2j9h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J9H FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[10gs\|10gs]], [[11gs\|11gs]], [[12gs\|12gs]], [[13gs\|13gs]], [[14gs\|14gs]], [[16gs\|16gs]], [[17gs\|17gs]], [[18gs\|18gs]], [[19gs\|19gs]], [[1aqv\|1aqv]], [[1aqw\|1aqw]], [[1aqx\|1aqx]], [[1eog\|1eog]], [[1eoh\|1eoh]], [[1gss\|1gss]], [[1kbn\|1kbn]], [[1lbk\|1lbk]], [[1md3\|1md3]], [[1md4\|1md4]], [[1pgt\|1pgt]], [[1px6\|1px6]], [[1px7\|1px7]], [[1zgn\|1zgn]], [[20gs\|20gs]], [[21gs\|21gs]], [[22gs\|22gs]], [[2a2r\|2a2r]], [[2a2s\|2a2s]], [[2gss\|2gss]], [[2pgt\|2pgt]], [[3gss\|3gss]], [[3pgt\|3pgt]], [[4gss\|4gss]], [[4pgt\|4pgt]], [[5gss\|5gss]], [[6gss\|6gss]], [[7gss\|7gss]], [[8gss\|8gss]], [[9gss\|9gss]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2j9h RCSB], [http://www.ebi.ac.uk/pdbsum/2j9h PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j9/2j9h_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The loop following helix alpha2 in glutathione transferase P1-1 has two conserved residues, Cys48 and Tyr50, important for glutathione (GSH) binding and catalytic activity. Chemical modification of Cys48 thwarts the catalytic activity of the enzyme, and mutation of Tyr50 generally decreases the k(cat) value and the affinity for GSH in a differential manner. Cys48 and Tyr50 were targeted by site-specific mutations and chemical modifications in order to investigate how the alpha2 loop modulates GSH binding and catalysis. Mutation of Cys48 into Ala increased K(M)(GSH) 24-fold and decreased the binding energy of GSH by 1.5 kcal/mol. Furthermore, the protein stability against thermal inactivation and chemical denaturation decreased. The crystal structure of the Cys-free variant was determined, and its similarity to the wild-type structure suggests that the mutation of Cys48 increases the flexibility of the alpha2 loop rather than dislocating the GSH-interacting residues. On the other hand, replacement of Tyr50 with Cys, producing mutant Y50C, increased the Gibbs free energy of the catalyzed reaction by 4.8 kcal/mol, lowered the affinity for S-hexyl glutathione by 2.2 kcal/mol, and decreased the thermal stability. The targeted alkylation of Cys50 in Y50C increased the affinity for GSH and protein stability. Characterization of the most active alkylated variants, S-n-butyl-, S-n-pentyl-, and S-cyclobutylmethyl-Y50C, indicated that the affinity for GSH is restored by stabilizing the alpha2 loop through positioning of the key residue into the lock structure of the neighboring subunit. In addition, k(cat) can be further modulated by varying the structure of the key residue side chain, which impinges on the rate-limiting step of catalysis.

-	~~{{STRUCTURE_2j9h\| PDB=2j9h \| SCENE= }}~~	+	Modulating catalytic activity by unnatural amino acid residues in a GSH-binding loop of GST P1-1.,Hegazy UM, Tars K, Hellman U, Mannervik B J Mol Biol. 2008 Feb 22;376(3):811-26. Epub 2007 Dec 14. PMID:18177897<ref>PMID:18177897</ref>

-	~~===CRYSTAL STRUCTURE OF HUMAN GLUTATHIONE-S-TRANSFERASE P1-1 CYS-FREE MUTANT IN COMPLEX WITH S-HEXYLGLUTATHIONE AT 2.4 A RESOLUTION===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18177897}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2j9h]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2J9H OCA].~~	+

	==See Also==		==See Also==
	*[[Glutathione S-transferase\|Glutathione S-transferase]]		*[[Glutathione S-transferase\|Glutathione S-transferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018177897<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Glutathione transferase]]		[[Category: Glutathione transferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]