2jbu - Revision history

OCA at 14:41, 13 December 2023

OCA — Wed, 13 Dec 2023 14:41:58 GMT

←Older revision		Revision as of 14:41, 13 December 2023
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	<StructureSection load='2jbu' size='340' side='right'caption='[[2jbu]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2jbu' size='340' side='right'caption='[[2jbu]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jbu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~] and [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JBU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jbu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JBU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DIO:1~~,~~4-DIETHYLENE+DIOXIDE'>DIO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Insulysin Insulysin]~~, ~~with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.4.24.56 3.4.24.56]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jbu OCA], [https://pdbe.org/2jbu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jbu RCSB], [https://www.ebi.ac.uk/pdbsum/2jbu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jbu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jbu OCA], [https://pdbe.org/2jbu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jbu RCSB], [https://www.ebi.ac.uk/pdbsum/2jbu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jbu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IDE_HUMAN IDE_HUMAN] Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.<ref>PMID:10684867</ref> <ref>PMID:17613531</ref> <ref>PMID:18986166</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
-	[[Category: ~~Human]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Insulysin~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Im, H]]	+	[[Category: Im H]]
-	[[Category: Shen, Y]]	+	[[Category: Shen Y]]
-	[[Category: Tang~~, W J]]~~	+	[[Category: Tang WJ]]
-	~~[[Category: Enzyme assay]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Insulin degrading enzyme]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Metalloprotease]]~~	+
-	~~[[Category: Protease]]~~	+
-	~~[[Category: Zinc~~]]	+

OCA at 20:44, 20 October 2021

OCA — Wed, 20 Oct 2021 20:44:37 GMT

←Older revision		Revision as of 20:44, 20 October 2021
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	<StructureSection load='2jbu' size='340' side='right'caption='[[2jbu]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2jbu' size='340' side='right'caption='[[2jbu]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jbu]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2JBU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jbu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JBU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Insulysin Insulysin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.56 3.4.24.56] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Insulysin Insulysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.56 3.4.24.56] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jbu OCA], [~~http~~://pdbe.org/2jbu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2jbu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2jbu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2jbu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jbu OCA], [https://pdbe.org/2jbu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jbu RCSB], [https://www.ebi.ac.uk/pdbsum/2jbu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jbu ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 19:46, 11 December 2019

OCA — Wed, 11 Dec 2019 19:46:15 GMT

←Older revision		Revision as of 19:46, 11 December 2019
Line 1:		Line 1:

	==Crystal structure of human insulin degrading enzyme complexed with co- purified peptides.==		==Crystal structure of human insulin degrading enzyme complexed with co- purified peptides.==
-	<StructureSection load='2jbu' size='340' side='right' caption='[[2jbu]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='2jbu' size='340' side='right'caption='[[2jbu]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2jbu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JBU FirstGlance]. <br>		<table><tr><td colspan='2'>[[2jbu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JBU FirstGlance]. <br>
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	==See Also==		==See Also==
-	*[[Insulin-~~Degrading Enzyme~~\|Insulin-~~Degrading Enzyme~~]]	+	*[[Insulin-degrading enzyme 3D structures\|Insulin-degrading enzyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Human]]		[[Category: Human]]
	[[Category: Insulysin]]		[[Category: Insulysin]]
		+	[[Category: Large Structures]]
	[[Category: Im, H]]		[[Category: Im, H]]
	[[Category: Shen, Y]]		[[Category: Shen, Y]]

OCA at 05:34, 8 March 2018

OCA — Thu, 08 Mar 2018 05:34:07 GMT

←Older revision		Revision as of 05:34, 8 March 2018
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-	==~~CRYSTAL STRUCTURE OF HUMAN INSULIN DEGRADING ENZYME COMPLEXED WITH CO~~-~~PURIFIED PEPTIDES~~.==	+
		+	==Crystal structure of human insulin degrading enzyme complexed with co- purified peptides.==
	<StructureSection load='2jbu' size='340' side='right' caption='[[2jbu]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2jbu' size='340' side='right' caption='[[2jbu]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Insulysin Insulysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.56 3.4.24.56] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Insulysin Insulysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.56 3.4.24.56] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jbu OCA], [http://pdbe.org/2jbu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jbu RCSB], [http://www.ebi.ac.uk/pdbsum/2jbu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jbu OCA], [http://pdbe.org/2jbu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jbu RCSB], [http://www.ebi.ac.uk/pdbsum/2jbu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jbu ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jb/2jbu_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jb/2jbu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	[[Category: Metalloprotease]]		[[Category: Metalloprotease]]
	[[Category: Protease]]		[[Category: Protease]]
		+	[[Category: Zinc]]

OCA at 19:14, 8 February 2016

OCA — Mon, 08 Feb 2016 19:14:43 GMT

←Older revision		Revision as of 19:14, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jbu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:35, 10 September 2015

OCA — Thu, 10 Sep 2015 14:35:15 GMT

←Older revision		Revision as of 14:35, 10 September 2015
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	<StructureSection load='2jbu' size='340' side='right' caption='[[2jbu]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2jbu' size='340' side='right' caption='[[2jbu]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jbu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli] and [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JBU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jbu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JBU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Insulysin Insulysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.56 3.4.24.56] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Insulysin Insulysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.56 3.4.24.56] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jbu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jbu RCSB], [http://www.ebi.ac.uk/pdbsum/2jbu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jbu OCA], [http://pdbe.org/2jbu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jbu RCSB], [http://www.ebi.ac.uk/pdbsum/2jbu PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2jbu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Insulysin]]		[[Category: Insulysin]]
	[[Category: Im, H]]		[[Category: Im, H]]

OCA at 16:01, 19 January 2015

OCA — Mon, 19 Jan 2015 16:01:50 GMT

←Older revision		Revision as of 16:01, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2jbu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JBU FirstGlance]. <br>		<table><tr><td colspan='2'>[[2jbu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JBU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Insulysin Insulysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.56 3.4.24.56] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Insulysin Insulysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.56 3.4.24.56] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jbu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jbu RCSB], [http://www.ebi.ac.uk/pdbsum/2jbu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jbu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jbu RCSB], [http://www.ebi.ac.uk/pdbsum/2jbu PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Insulysin]]		[[Category: Insulysin]]
-	[[Category: Im, H.]]	+	[[Category: Im, H]]
-	[[Category: Shen, Y.]]	+	[[Category: Shen, Y]]
-	[[Category: Tang, W J.]]	+	[[Category: Tang, W J]]
	[[Category: Enzyme assay]]		[[Category: Enzyme assay]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 05:25, 29 September 2014

OCA — Mon, 29 Sep 2014 05:25:43 GMT

←Older revision		Revision as of 05:25, 29 September 2014
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-	[[~~Image~~:2jbu.~~png\|left~~\|200px]]	+	==CRYSTAL STRUCTURE OF HUMAN INSULIN DEGRADING ENZYME COMPLEXED WITH CO-PURIFIED PEPTIDES.==
		+	<StructureSection load='2jbu' size='340' side='right' caption='[[2jbu]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2jbu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JBU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Insulysin Insulysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.56 3.4.24.56] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jbu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jbu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jbu RCSB], [http://www.ebi.ac.uk/pdbsum/2jbu PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jb/2jbu_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Insulin-degrading enzyme (IDE) is a zinc metalloprotease that hydrolyzes amyloid-beta (Abeta) and insulin, which are peptides associated with Alzheimer disease (AD) and diabetes, respectively. Our previous structural analysis of substrate-bound human 113-kDa IDE reveals that the N- and C-terminal domains of IDE, IDE-N and IDE-C, make substantial contact to form an enclosed catalytic chamber to entrap its substrates. Furthermore, IDE undergoes a switch between the closed and open conformations for catalysis. Here we report a substrate-free IDE structure in its closed conformation, revealing the molecular details of the active conformation of the catalytic site of IDE and new insights as to how the closed conformation of IDE may be kept in its resting, inactive conformation. We also show that Abeta is degraded more efficiently by IDE carrying destabilizing mutations at the interface of IDE-N and IDE-C (D426C and K899C), resulting in an increase in Vmax with only minimal changes to Km. Because ATP is known to activate the ability of IDE to degrade short peptides, we investigated the interaction between ATP and activating mutations. We found that these mutations rendered IDE less sensitive to ATP activation, suggesting that ATP might facilitate the transition from the closed state to the open conformation. Consistent with this notion, we found that ATP induced an increase in hydrodynamic radius, a shift in electrophoretic mobility, and changes in secondary structure. Together, our results highlight the importance of the closed conformation for regulating the activity of IDE and provide new molecular details that will facilitate the development of activators and inhibitors of IDE.

-	~~{{STRUCTURE_2jbu\| PDB=2jbu \| SCENE= }}~~	+	Structure of substrate-free human insulin-degrading enzyme (IDE) and biophysical analysis of ATP-induced conformational switch of IDE.,Im H, Manolopoulou M, Malito E, Shen Y, Zhao J, Neant-Fery M, Sun CY, Meredith SC, Sisodia SS, Leissring MA, Tang WJ J Biol Chem. 2007 Aug 31;282(35):25453-63. Epub 2007 Jul 5. PMID:17613531<ref>PMID:17613531</ref>

-	~~===CRYSTAL STRUCTURE OF HUMAN INSULIN DEGRADING ENZYME COMPLEXED WITH CO-PURIFIED PEPTIDES.===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17613531}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2jbu]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli] and [http://en~~.~~wikipedia.org~~/~~wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA].~~	+

	==See Also==		==See Also==
	*[[Insulin-Degrading Enzyme\|Insulin-Degrading Enzyme]]		*[[Insulin-Degrading Enzyme\|Insulin-Degrading Enzyme]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:017613531<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 11:11, 20 October 2012

OCA — Sat, 20 Oct 2012 11:11:46 GMT

←Older revision		Revision as of 11:11, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[2jbu]] is a 4 chain structure ~~of [[Insulin-Degrading Enzyme]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA].	+	[[2jbu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JBU OCA].

	==See Also==		==See Also==

OCA: Protected "2jbu" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 04:32:29 GMT

Protected "2jbu" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:32, 26 July 2012