2jk8 - Revision history

OCA at 14:52, 13 December 2023

OCA — Wed, 13 Dec 2023 14:52:32 GMT

←Older revision		Revision as of 14:52, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='2jk8' size='340' side='right'caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2jk8' size='340' side='right'caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_49882 Atcc 49882~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JK8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JK8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vy3\|2vy3]], [[2vza\|2vza]]</div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [https://pdbe.org/2jk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [https://www.ebi.ac.uk/pdbsum/2jk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jk8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [https://pdbe.org/2jk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [https://www.ebi.ac.uk/pdbsum/2jk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jk8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BEPA_BARHE BEPA_BARHE]] Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific residue of host target proteins.<ref>PMID:21213248</ref>	+	[https://www.uniprot.org/uniprot/BEPA_BARHE BEPA_BARHE] Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific residue of host target proteins.<ref>PMID:21213248</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 49882~~]]	+	[[Category: Bartonella henselae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Palanivelu~~, D V~~]]	+	[[Category: Palanivelu DV]]
-	[[Category: Schirmer, T]]	+	[[Category: Schirmer T]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Fic domain]]~~	+
-	~~[[Category: Ob fold]]~~	+
-	~~[[Category: Protein translocation]]~~	+
-	~~[[Category: Substrate protein]]~~	+
-	~~[[Category: T4ss~~]]	+

OCA at 08:13, 19 January 2022

OCA — Wed, 19 Jan 2022 08:13:16 GMT

←Older revision		Revision as of 08:13, 19 January 2022
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	<StructureSection load='2jk8' size='340' side='right'caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2jk8' size='340' side='right'caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_49882 Atcc 49882]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2JK8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_49882 Atcc 49882]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JK8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vy3\|2vy3]], [[2vza\|2vza]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vy3\|2vy3]], [[2vza\|2vza]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [~~http~~://pdbe.org/2jk8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2jk8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2jk8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [https://pdbe.org/2jk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [https://www.ebi.ac.uk/pdbsum/2jk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jk8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BEPA_BARHE BEPA_BARHE]] Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific residue of host target proteins.<ref>PMID:21213248</ref>	+	[[https://www.uniprot.org/uniprot/BEPA_BARHE BEPA_BARHE]] Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific residue of host target proteins.<ref>PMID:21213248</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:53, 15 April 2020

OCA — Wed, 15 Apr 2020 07:53:56 GMT

←Older revision		Revision as of 07:53, 15 April 2020
Line 1:		Line 1:

	==Type IV secretion system effector protein BepA complexed with a pyrophosphate moiety==		==Type IV secretion system effector protein BepA complexed with a pyrophosphate moiety==
-	<StructureSection load='2jk8' size='340' side='right' caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='2jk8' size='340' side='right'caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_49882 Atcc 49882]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2JK8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_49882 Atcc 49882]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2JK8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vy3\|2vy3]], [[2vza\|2vza]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vy3\|2vy3]], [[2vza\|2vza]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://pdbe.org/2jk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jk8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://pdbe.org/2jk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jk8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 49882]]		[[Category: Atcc 49882]]
		+	[[Category: Large Structures]]
	[[Category: Palanivelu, D V]]		[[Category: Palanivelu, D V]]
	[[Category: Schirmer, T]]		[[Category: Schirmer, T]]

OCA at 06:51, 11 July 2018

OCA — Wed, 11 Jul 2018 06:51:49 GMT

←Older revision		Revision as of 06:51, 11 July 2018
Line 1:		Line 1:
-	==~~TYPE~~ IV ~~SECRETION SYSTEM EFFECTOR PROTEIN BEPA COMPLEXED WITH A PYROPHOSPHATE MOIETY~~==	+
		+	==Type IV secretion system effector protein BepA complexed with a pyrophosphate moiety==
	<StructureSection load='2jk8' size='340' side='right' caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2jk8' size='340' side='right' caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vy3\|2vy3]], [[2vza\|2vza]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vy3\|2vy3]], [[2vza\|2vza]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://pdbe.org/2jk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://pdbe.org/2jk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jk8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jk/2jk8_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jk/2jk8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jk8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:19, 12 September 2015

OCA — Sat, 12 Sep 2015 02:19:48 GMT

←Older revision		Revision as of 02:19, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='2jk8' size='340' side='right' caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2jk8' size='340' side='right' caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bartonella_henselae Bartonella henselae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JK8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_49882 Atcc 49882]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JK8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vy3\|2vy3]], [[2vza\|2vza]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vy3\|2vy3]], [[2vza\|2vza]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://pdbe.org/2jk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q6G2A9_BARHE Q6G2A9_BARHE~~]] Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific residue of host target proteins.	+	[[http://www.uniprot.org/uniprot/BEPA_BARHE BEPA_BARHE]] Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific residue of host target proteins.<ref>PMID:21213248</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2jk8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bartonella henselae~~]]	+	[[Category: Atcc 49882]]
	[[Category: Palanivelu, D V]]		[[Category: Palanivelu, D V]]
	[[Category: Schirmer, T]]		[[Category: Schirmer, T]]

OCA at 22:36, 25 December 2014

OCA — Thu, 25 Dec 2014 22:36:40 GMT

←Older revision		Revision as of 22:36, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JK8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JK8 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vy3\|2vy3]], [[2vza\|2vza]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vy3\|2vy3]], [[2vza\|2vza]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q6G2A9_BARHE Q6G2A9_BARHE]] Adenylyltransferase involved in virulence by mediating the addition of adenosine 5'-monophosphate (AMP) to specific residue of host target proteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Bartonella henselae]]		[[Category: Bartonella henselae]]
-	[[Category: Palanivelu, D V.]]	+	[[Category: Palanivelu, D V]]
-	[[Category: Schirmer, T.]]	+	[[Category: Schirmer, T]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Fic domain]]		[[Category: Fic domain]]

OCA at 11:41, 3 October 2014

OCA — Fri, 03 Oct 2014 11:41:04 GMT

←Older revision		Revision as of 11:41, 3 October 2014
Line 2:		Line 2:
	<StructureSection load='2jk8' size='340' side='right' caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2jk8' size='340' side='right' caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. <br>	+	<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JK8 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene><br>		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene><br>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vy3\|2vy3]], [[2vza\|2vza]]</td></tr>		<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vy3\|2vy3]], [[2vza\|2vza]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum]</span></td></tr>
	<table>		<table>
Line 24:		Line 23:
	Fic domain catalyzed adenylylation: Insight provided by the structural analysis of the type IV secretion system effector BepA.,Palanivelu DV, Goepfert A, Meury M, Guye P, Dehio C, Schirmer T Protein Sci. 2011 Jan 6. PMID:21213248<ref>PMID:21213248</ref>		Fic domain catalyzed adenylylation: Insight provided by the structural analysis of the type IV secretion system effector BepA.,Palanivelu DV, Goepfert A, Meury M, Guye P, Dehio C, Schirmer T Protein Sci. 2011 Jan 6. PMID:21213248<ref>PMID:21213248</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 10:02, 1 May 2014

OCA — Thu, 01 May 2014 10:02:39 GMT

←Older revision		Revision as of 10:02, 1 May 2014
Line 2:		Line 2:
	<StructureSection load='2jk8' size='340' side='right' caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='2jk8' size='340' side='right' caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. <br>	+	<table><tr><td colspan='2'>[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. <br>
-	<b>[[Ligand\|Ligands:]]</b> <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene><br>	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene><br>
-	<b>[[Related_structure\|Related:]]</b> [[2vy3\|2vy3]], [[2vza\|2vza]]<br>	+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vy3\|2vy3]], [[2vza\|2vza]]</td></tr>
-	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
-	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum]</span><br>	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum]</span></td></tr>
		+	<table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
-	[[Image:Consurf_key_small.gif\|right]]	+	[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
Line 17:		Line 18:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	Numerous bacterial pathogens subvert cellular functions of eukaryotic host cells by the injection of effector proteins via dedicated secretion systems. The type IV secretion system (T4SS) effector protein BepA from Bartonella henselae is composed of an N-terminal Fic domain and a C-terminal BID domain, the latter being responsible for T4SS-mediated translocation into host cells. A proteolysis resistant fragment (residues 10 to 302) that includes the Fic domain shows auto-adenylylation activity and adenylyl transfer onto Hela cell extract proteins as demonstrated by autoradiography upon incubation with alpha-[(32)P]-ATP. Its crystal structure, determined to 2.9 A resolution by the SeMet-SAD method, exhibits the canonical Fic fold including the HPFxxGNGRxxR signature motif with several elaborations in loop regions and an additional beta-rich domain at the C-terminus. Upon crystal soaking with ATP/Mg(2+), additional electron density indicated the presence of a PP(i)/Mg(2+) moiety, the side product of the adenylylation reaction, in the anion binding nest of the signature motif. Based on this information and that of the recent structure of IbpA(Fic2) in complex with the eukaryotic target protein Cdc42, we present a detailed model for the ternary complex of Fic with the two substrates, ATP/Mg(2+) and target tyrosine. The model is consistent with an in-line nucleophilic attack of the deprotonated side-chain hydroxyl group onto the alpha-phosphorus of the nucleotide to accomplish AMP transfer. Furthermore, a general, sequence independent mechanism of target positioning through antiparallel beta-strand interactions between enzyme and target is suggested.		Numerous bacterial pathogens subvert cellular functions of eukaryotic host cells by the injection of effector proteins via dedicated secretion systems. The type IV secretion system (T4SS) effector protein BepA from Bartonella henselae is composed of an N-terminal Fic domain and a C-terminal BID domain, the latter being responsible for T4SS-mediated translocation into host cells. A proteolysis resistant fragment (residues 10 to 302) that includes the Fic domain shows auto-adenylylation activity and adenylyl transfer onto Hela cell extract proteins as demonstrated by autoradiography upon incubation with alpha-[(32)P]-ATP. Its crystal structure, determined to 2.9 A resolution by the SeMet-SAD method, exhibits the canonical Fic fold including the HPFxxGNGRxxR signature motif with several elaborations in loop regions and an additional beta-rich domain at the C-terminus. Upon crystal soaking with ATP/Mg(2+), additional electron density indicated the presence of a PP(i)/Mg(2+) moiety, the side product of the adenylylation reaction, in the anion binding nest of the signature motif. Based on this information and that of the recent structure of IbpA(Fic2) in complex with the eukaryotic target protein Cdc42, we present a detailed model for the ternary complex of Fic with the two substrates, ATP/Mg(2+) and target tyrosine. The model is consistent with an in-line nucleophilic attack of the deprotonated side-chain hydroxyl group onto the alpha-phosphorus of the nucleotide to accomplish AMP transfer. Furthermore, a general, sequence independent mechanism of target positioning through antiparallel beta-strand interactions between enzyme and target is suggested.
Line 23:		Line 25:

	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:27, 30 April 2014

OCA — Wed, 30 Apr 2014 10:27:55 GMT

←Older revision		Revision as of 10:27, 30 April 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. <br>		[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. <br>
-	<b>Related:</b> [[2vy3\|2vy3]], [[2vza\|2vza]]<br>	+	<b>[[Ligand\|Ligands:]]</b> <scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene><br>
		+	<b>[[Related_structure\|Related:]]</b> [[2vy3\|2vy3]], [[2vza\|2vza]]<br>
	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>		<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
		+	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jk8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jk8 RCSB], [http://www.ebi.ac.uk/pdbsum/2jk8 PDBsum]</span><br>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|right]]		[[Image:Consurf_key_small.gif\|right]]

OCA at 08:45, 30 April 2014

OCA — Wed, 30 Apr 2014 08:45:32 GMT

←Older revision		Revision as of 08:45, 30 April 2014
Line 1:		Line 1:
-	[[Image:~~2jk8~~.~~png~~\|~~left~~\|~~200px~~]]	+	==TYPE IV SECRETION SYSTEM EFFECTOR PROTEIN BEPA COMPLEXED WITH A PYROPHOSPHATE MOIETY==
		+	<StructureSection load='2jk8' size='340' side='right' caption='[[2jk8]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	[[2jk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA]. <br>
		+	<b>Related:</b> [[2vy3\|2vy3]], [[2vza\|2vza]]<br>
		+	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jk/2jk8_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	== Publication Abstract from PubMed ==
		+	Numerous bacterial pathogens subvert cellular functions of eukaryotic host cells by the injection of effector proteins via dedicated secretion systems. The type IV secretion system (T4SS) effector protein BepA from Bartonella henselae is composed of an N-terminal Fic domain and a C-terminal BID domain, the latter being responsible for T4SS-mediated translocation into host cells. A proteolysis resistant fragment (residues 10 to 302) that includes the Fic domain shows auto-adenylylation activity and adenylyl transfer onto Hela cell extract proteins as demonstrated by autoradiography upon incubation with alpha-[(32)P]-ATP. Its crystal structure, determined to 2.9 A resolution by the SeMet-SAD method, exhibits the canonical Fic fold including the HPFxxGNGRxxR signature motif with several elaborations in loop regions and an additional beta-rich domain at the C-terminus. Upon crystal soaking with ATP/Mg(2+), additional electron density indicated the presence of a PP(i)/Mg(2+) moiety, the side product of the adenylylation reaction, in the anion binding nest of the signature motif. Based on this information and that of the recent structure of IbpA(Fic2) in complex with the eukaryotic target protein Cdc42, we present a detailed model for the ternary complex of Fic with the two substrates, ATP/Mg(2+) and target tyrosine. The model is consistent with an in-line nucleophilic attack of the deprotonated side-chain hydroxyl group onto the alpha-phosphorus of the nucleotide to accomplish AMP transfer. Furthermore, a general, sequence independent mechanism of target positioning through antiparallel beta-strand interactions between enzyme and target is suggested.

-	~~<!--~~	+	Fic domain catalyzed adenylylation: Insight provided by the structural analysis of the type IV secretion system effector BepA.,Palanivelu DV, Goepfert A, Meury M, Guye P, Dehio C, Schirmer T Protein Sci. 2011 Jan 6. PMID:21213248<ref>PMID:21213248</ref>
-	~~The line below this paragraph, containing "STRUCTURE_2jk8", creates~~ the ~~"Structure Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_2jk8\| PDB=2jk8 \| SCENE= }}~~	+

-	~~===TYPE IV SECRETION SYSTEM EFFECTOR PROTEIN BEPA COMPLEXED WITH A PYROPHOSPHATE MOIETY===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	== References ==
-		+	<references/>
-	~~<!--~~	+	__TOC__
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21213248}}, adds~~ the ~~Publication Abstract to the page~~	+	</StructureSection>
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21213248 is the PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_21213248}}~~	+
-		+
-	==~~About this Structure~~==	+
-	~~[[2jk8]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Bartonella_henselae Bartonella henselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JK8 OCA].~~	+
-		+
-	~~==Reference==~~	+
-	<~~ref group="xtra">PMID:021213248</ref><references group="xtra"~~/>	+
	[[Category: Bartonella henselae]]		[[Category: Bartonella henselae]]
	[[Category: Palanivelu, D V.]]		[[Category: Palanivelu, D V.]]