2jml - Revision history

OCA at 10:07, 20 December 2023

OCA — Wed, 20 Dec 2023 10:07:16 GMT

←Older revision		Revision as of 10:07, 20 December 2023
Line 1:		Line 1:

	==Solution structure of the N-terminal domain of CarA repressor==		==Solution structure of the N-terminal domain of CarA repressor==
-	<StructureSection load='2jml' size='340' side='right'caption='[[2jml~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2jml' size='340' side='right'caption='[[2jml]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myxxd Myxxd~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JML FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus_DK_1622 Myxococcus xanthus DK 1622]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JML FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [https://pdbe.org/2jml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [https://www.ebi.ac.uk/pdbsum/2jml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jml ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [https://pdbe.org/2jml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [https://www.ebi.ac.uk/pdbsum/2jml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jml ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q1DDV9_MYXXD Q1DDV9_MYXXD]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myxxd~~]]	+	[[Category: Myxococcus xanthus DK 1622]]
-	[[Category: Elias-Arnanz, M]]	+	[[Category: Elias-Arnanz M]]
-	[[Category: Gonzalez, C]]	+	[[Category: Gonzalez C]]
-	[[Category: Jimenez, M]]	+	[[Category: Jimenez M]]
-	[[Category: Murillo~~, F J~~]]	+	[[Category: Murillo FJ]]
-	[[Category: Padmanabhan, S]]	+	[[Category: Padmanabhan S]]
-	[[Category: Perez-Marin~~, M C~~]]	+	[[Category: Perez-Marin MC]]
-	[[Category: Rico, M]]	+	[[Category: Rico M]]
-	~~[[Category: Anti-repressor]]~~	+
-	~~[[Category: Carotenogenesis]]~~	+
-	~~[[Category: Merr]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 08:15, 19 January 2022

OCA — Wed, 19 Jan 2022 08:15:21 GMT

←Older revision		Revision as of 08:15, 19 January 2022
Line 3:		Line 3:
	<StructureSection load='2jml' size='340' side='right'caption='[[2jml]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2jml' size='340' side='right'caption='[[2jml]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Myxxd Myxxd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2JML FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxxd Myxxd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JML FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [~~http~~://pdbe.org/2jml PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2jml PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2jml ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [https://pdbe.org/2jml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [https://www.ebi.ac.uk/pdbsum/2jml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jml ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:56, 15 April 2020

OCA — Wed, 15 Apr 2020 07:56:06 GMT

←Older revision		Revision as of 07:56, 15 April 2020
Line 1:		Line 1:

	==Solution structure of the N-terminal domain of CarA repressor==		==Solution structure of the N-terminal domain of CarA repressor==
-	<StructureSection load='2jml' size='340' side='right' caption='[[2jml]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2jml' size='340' side='right'caption='[[2jml]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myxxd Myxxd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2JML FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myxxd Myxxd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2JML FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [http://pdbe.org/2jml PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [http://www.ebi.ac.uk/pdbsum/2jml PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jml ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [http://pdbe.org/2jml PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [http://www.ebi.ac.uk/pdbsum/2jml PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jml ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 29:		Line 29:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Myxxd]]		[[Category: Myxxd]]
	[[Category: Elias-Arnanz, M]]		[[Category: Elias-Arnanz, M]]

OCA at 07:02, 11 July 2018

OCA — Wed, 11 Jul 2018 07:02:55 GMT

←Older revision		Revision as of 07:02, 11 July 2018
Line 1:		Line 1:
		+
	==Solution structure of the N-terminal domain of CarA repressor==		==Solution structure of the N-terminal domain of CarA repressor==
	<StructureSection load='2jml' size='340' side='right' caption='[[2jml]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2jml' size='340' side='right' caption='[[2jml]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myxxd Myxxd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JML FirstGlance]. <br>		<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myxxd Myxxd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JML FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [http://pdbe.org/2jml PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [http://www.ebi.ac.uk/pdbsum/2jml PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [http://pdbe.org/2jml PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [http://www.ebi.ac.uk/pdbsum/2jml PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jml ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 9:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/2jml_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/2jml_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 19:10, 9 February 2016

OCA — Tue, 09 Feb 2016 19:10:35 GMT

←Older revision		Revision as of 19:10, 9 February 2016
Line 13:		Line 13:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jml ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:13, 11 September 2015

OCA — Fri, 11 Sep 2015 01:13:30 GMT

←Older revision		Revision as of 01:13, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2jml' size='340' side='right' caption='[[2jml]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2jml' size='340' side='right' caption='[[2jml]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Myxococcus_xanthus_dk_1622 Myxococcus xanthus dk 1622~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JML FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myxxd Myxxd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JML FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [http://www.ebi.ac.uk/pdbsum/2jml PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [http://pdbe.org/2jml PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [http://www.ebi.ac.uk/pdbsum/2jml PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 23:		Line 23:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2jml" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Myxococcus xanthus dk 1622~~]]	+	[[Category: Myxxd]]
	[[Category: Elias-Arnanz, M]]		[[Category: Elias-Arnanz, M]]
	[[Category: Gonzalez, C]]		[[Category: Gonzalez, C]]

OCA at 18:18, 19 January 2015

OCA — Mon, 19 Jan 2015 18:18:57 GMT

←Older revision		Revision as of 18:18, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myxococcus_xanthus_dk_1622 Myxococcus xanthus dk 1622]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JML FirstGlance]. <br>		<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myxococcus_xanthus_dk_1622 Myxococcus xanthus dk 1622]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JML FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [http://www.ebi.ac.uk/pdbsum/2jml PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [http://www.ebi.ac.uk/pdbsum/2jml PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Myxococcus xanthus dk 1622]]		[[Category: Myxococcus xanthus dk 1622]]
-	[[Category: Elias-Arnanz, M.]]	+	[[Category: Elias-Arnanz, M]]
-	[[Category: Gonzalez, C.]]	+	[[Category: Gonzalez, C]]
-	[[Category: Jimenez, M.]]	+	[[Category: Jimenez, M]]
-	[[Category: Murillo, F J.]]	+	[[Category: Murillo, F J]]
-	[[Category: Padmanabhan, S.]]	+	[[Category: Padmanabhan, S]]
-	[[Category: Perez-Marin, M C.]]	+	[[Category: Perez-Marin, M C]]
-	[[Category: Rico, M.]]	+	[[Category: Rico, M]]
	[[Category: Anti-repressor]]		[[Category: Anti-repressor]]
	[[Category: Carotenogenesis]]		[[Category: Carotenogenesis]]
	[[Category: Merr]]		[[Category: Merr]]
	[[Category: Transcription]]		[[Category: Transcription]]

OCA at 10:45, 30 September 2014

OCA — Tue, 30 Sep 2014 10:45:45 GMT

←Older revision		Revision as of 10:45, 30 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2jml.~~png\|left~~\|200px]]	+	==Solution structure of the N-terminal domain of CarA repressor==
		+	<StructureSection load='2jml' size='340' side='right' caption='[[2jml]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myxococcus_xanthus_dk_1622 Myxococcus xanthus dk 1622]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JML FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [http://www.ebi.ac.uk/pdbsum/2jml PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/2jml_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Blue light induces carotenogenesis in Myxococcus xanthus. The carB operon encodes all but one of the structural genes involved, and its expression is regulated by the CarA-CarS repressor-antirepressor pair. In the dark, CarA-operator binding represses carB. CarS, produced on illumination, interacts physically with CarA to dismantle the CarA-operator complex and activate carB. Both operator and CarS bind to the autonomously folded N-terminal domain of CarA, CarA(Nter), which in excess represses carB. Here, we report the NMR structure of CarA(Nter), and map residues that interact with operator and CarS by NMR chemical shift perturbations, and in vivo and in vitro analyses of site-directed mutants. We show CarA(Nter) adopts the winged-helix topology of MerR-family DNA-binding domains, and conserves the majority of the helix-turn-helix and wing contacts with DNA. Tellingly, helix alpha2 in CarA, a key element in operator DNA recognition, is also critical for interaction with CarS, implying that the CarA-CarS protein-protein and the CarA-operator protein-DNA interfaces overlap. Thus, binding of CarA to operator and to antirepressor are mutually exclusive, and CarA may discern structural features in the acidic CarS protein that resemble operator DNA. Repressor inactivation by occluding the DNA-binding region may be a recurrent mechanism of action for acidic antirepressors.

-	~~{{STRUCTURE_2jml\| PDB=2jml \| SCENE= }}~~	+	Structural basis for operator and antirepressor recognition by Myxococcus xanthus CarA repressor.,Navarro-Aviles G, Jimenez MA, Perez-Marin MC, Gonzalez C, Rico M, Murillo FJ, Elias-Arnanz M, Padmanabhan S Mol Microbiol. 2007 Feb;63(4):980-94. PMID:17233828<ref>PMID:17233828</ref>

-	~~===Solution structure~~ of the ~~N-terminal domain of CarA repressor===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17233828}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2jml]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Myxococcus_xanthus_dk_1622 Myxococcus xanthus dk 1622]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017233828<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Myxococcus xanthus dk 1622]]		[[Category: Myxococcus xanthus dk 1622]]
	[[Category: Elias-Arnanz, M.]]		[[Category: Elias-Arnanz, M.]]

OCA: Protected "2jml" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 12:34:09 GMT

Protected "2jml" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:34, 7 January 2013

OCA at 12:34, 7 January 2013

OCA — Mon, 07 Jan 2013 12:34:03 GMT

←Older revision		Revision as of 12:34, 7 January 2013
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-	{{Seed}}
	[[Image:2jml.png\|left\|200px]]		[[Image:2jml.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2jml", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2jml\| PDB=2jml \| SCENE= }}		{{STRUCTURE_2jml\| PDB=2jml \| SCENE= }}

	===Solution structure of the N-terminal domain of CarA repressor===		===Solution structure of the N-terminal domain of CarA repressor===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17233828}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17233828 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17233828}}		{{ABSTRACT_PUBMED_17233828}}

	==About this Structure==		==About this Structure==
-	~~2JML~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/~~Myxococcus_xanthus~~ Myxococcus xanthus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA].	+	[[2jml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myxococcus_xanthus_dk_1622 Myxococcus xanthus dk 1622]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17233828~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017233828</ref><references group="xtra"/>
-	[[Category: Myxococcus xanthus]]	+	[[Category: Myxococcus xanthus dk 1622]]
	[[Category: Elias-Arnanz, M.]]		[[Category: Elias-Arnanz, M.]]
	[[Category: Gonzalez, C.]]		[[Category: Gonzalez, C.]]
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	[[Category: Carotenogenesis]]		[[Category: Carotenogenesis]]
	[[Category: Merr]]		[[Category: Merr]]
-		+	[[Category: Transcription]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 12:05:56 2009''~~	+