2k0n - Revision history

OCA at 19:08, 29 May 2024

OCA — Wed, 29 May 2024 19:08:34 GMT

←Older revision		Revision as of 19:08, 29 May 2024
Line 1:		Line 1:

	==Solution Structure of Yeast Gal11p kix domain==		==Solution Structure of Yeast Gal11p kix domain==
-	<StructureSection load='2k0n' size='340' side='right'caption='[[2k0n~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2k0n' size='340' side='right'caption='[[2k0n]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2k0n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K0N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2k0n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K0N FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>GAL11, ABE1, MED15, RAR3, SDS4, SPT13 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~4932 ATCC 18824])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0n OCA], [https://pdbe.org/2k0n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k0n RCSB], [https://www.ebi.ac.uk/pdbsum/2k0n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k0n ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0n OCA], [https://pdbe.org/2k0n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k0n RCSB], [https://www.ebi.ac.uk/pdbsum/2k0n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k0n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MED15_YEAST MED15_YEAST]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.<ref>PMID:16076843</ref> <ref>PMID:16109375</ref> <ref>PMID:16263706</ref>	+	[https://www.uniprot.org/uniprot/MED15_YEAST MED15_YEAST] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.<ref>PMID:16076843</ref> <ref>PMID:16109375</ref> <ref>PMID:16263706</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Arthanari, H~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Frueh, D P~~]]	+	[[Category: Arthanari H]]
-	[[Category: ~~Naar, A M~~]]	+	[[Category: Frueh DP]]
-	[[Category: ~~Wagner, G K~~]]	+	[[Category: Naar AM]]
-	[[Category: ~~Activator]]~~	+	[[Category: Wagner GK]]
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Protein]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription regulation~~]]	+

OCA at 08:04, 7 April 2021

OCA — Wed, 07 Apr 2021 08:04:32 GMT

←Older revision		Revision as of 08:04, 7 April 2021
Line 1:		Line 1:

	==Solution Structure of Yeast Gal11p kix domain==		==Solution Structure of Yeast Gal11p kix domain==
-	<StructureSection load='2k0n' size='340' side='right' caption='[[2k0n]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2k0n' size='340' side='right'caption='[[2k0n]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2k0n]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2K0N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2k0n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K0N FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL11, ABE1, MED15, RAR3, SDS4, SPT13 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL11, ABE1, MED15, RAR3, SDS4, SPT13 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0n OCA], [~~http~~://pdbe.org/2k0n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2k0n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2k0n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2k0n ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0n OCA], [https://pdbe.org/2k0n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k0n RCSB], [https://www.ebi.ac.uk/pdbsum/2k0n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k0n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MED15_YEAST MED15_YEAST]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.<ref>PMID:16076843</ref> <ref>PMID:16109375</ref> <ref>PMID:16263706</ref>	+	[[https://www.uniprot.org/uniprot/MED15_YEAST MED15_YEAST]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.<ref>PMID:16076843</ref> <ref>PMID:16109375</ref> <ref>PMID:16263706</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Arthanari, H]]		[[Category: Arthanari, H]]
	[[Category: Frueh, D P]]		[[Category: Frueh, D P]]

OCA at 10:16, 18 July 2018

OCA — Wed, 18 Jul 2018 10:16:57 GMT

←Older revision		Revision as of 10:16, 18 July 2018
Line 1:		Line 1:
		+
	==Solution Structure of Yeast Gal11p kix domain==		==Solution Structure of Yeast Gal11p kix domain==
	<StructureSection load='2k0n' size='340' side='right' caption='[[2k0n]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2k0n' size='340' side='right' caption='[[2k0n]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2k0n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K0N FirstGlance]. <br>		<table><tr><td colspan='2'>[[2k0n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K0N FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL11, ABE1, MED15, RAR3, SDS4, SPT13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL11, ABE1, MED15, RAR3, SDS4, SPT13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0n OCA], [http://pdbe.org/2k0n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k0n RCSB], [http://www.ebi.ac.uk/pdbsum/2k0n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0n OCA], [http://pdbe.org/2k0n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k0n RCSB], [http://www.ebi.ac.uk/pdbsum/2k0n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2k0n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/2k0n_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/2k0n_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k0n ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:48, 11 September 2015

OCA — Fri, 11 Sep 2015 15:48:06 GMT

←Older revision		Revision as of 15:48, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2k0n' size='340' side='right' caption='[[2k0n]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2k0n' size='340' side='right' caption='[[2k0n]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2k0n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K0N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2k0n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K0N FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL11, ABE1, MED15, RAR3, SDS4, SPT13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL11, ABE1, MED15, RAR3, SDS4, SPT13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k0n RCSB], [http://www.ebi.ac.uk/pdbsum/2k0n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0n OCA], [http://pdbe.org/2k0n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k0n RCSB], [http://www.ebi.ac.uk/pdbsum/2k0n PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2k0n" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Arthanari, H]]		[[Category: Arthanari, H]]
	[[Category: Frueh, D P]]		[[Category: Frueh, D P]]

OCA at 18:36, 25 December 2014

OCA — Thu, 25 Dec 2014 18:36:04 GMT

←Older revision		Revision as of 18:36, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2k0n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K0N FirstGlance]. <br>		<table><tr><td colspan='2'>[[2k0n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K0N FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL11, ABE1, MED15, RAR3, SDS4, SPT13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL11, ABE1, MED15, RAR3, SDS4, SPT13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k0n RCSB], [http://www.ebi.ac.uk/pdbsum/2k0n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k0n RCSB], [http://www.ebi.ac.uk/pdbsum/2k0n PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MED15_YEAST MED15_YEAST]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. It has an important role in the negative regulation of Ty transcription.<ref>PMID:16076843</ref> <ref>PMID:16109375</ref> <ref>PMID:16263706</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Arthanari, H.]]	+	[[Category: Arthanari, H]]
-	[[Category: Frueh, D P.]]	+	[[Category: Frueh, D P]]
-	[[Category: Naar, A M.]]	+	[[Category: Naar, A M]]
-	[[Category: Wagner, G K.]]	+	[[Category: Wagner, G K]]
	[[Category: Activator]]		[[Category: Activator]]
	[[Category: Nucleus]]		[[Category: Nucleus]]

OCA at 10:24, 30 September 2014

OCA — Tue, 30 Sep 2014 10:24:42 GMT

←Older revision		Revision as of 10:24, 30 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2k0n.~~png\|left~~\|200px]]	+	==Solution Structure of Yeast Gal11p kix domain==
		+	<StructureSection load='2k0n' size='340' side='right' caption='[[2k0n]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2k0n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K0N FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GAL11, ABE1, MED15, RAR3, SDS4, SPT13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k0n RCSB], [http://www.ebi.ac.uk/pdbsum/2k0n PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/2k0n_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multidrug resistance (MDR) is a serious complication during treatment of opportunistic fungal infections that frequently afflict immunocompromised individuals, such as transplant recipients and cancer patients undergoing cytotoxic chemotherapy. Improved knowledge of the molecular pathways controlling MDR in pathogenic fungi should facilitate the development of novel therapies to combat these intransigent infections. MDR is often caused by upregulation of drug efflux pumps by members of the fungal zinc-cluster transcription-factor family (for example Pdr1p orthologues). However, the molecular mechanisms are poorly understood. Here we show that Pdr1p family members in Saccharomyces cerevisiae and the human pathogen Candida glabrata directly bind to structurally diverse drugs and xenobiotics, resulting in stimulated expression of drug efflux pumps and induction of MDR. Notably, this is mechanistically similar to regulation of MDR in vertebrates by the PXR nuclear receptor, revealing an unexpected functional analogy of fungal and metazoan regulators of MDR. We have also uncovered a critical and specific role of the Gal11p/MED15 subunit of the Mediator co-activator and its activator-targeted KIX domain in antifungal/xenobiotic-dependent regulation of MDR. This detailed mechanistic understanding of a fungal nuclear receptor-like gene regulatory pathway provides novel therapeutic targets for the treatment of multidrug-resistant fungal infections.

-	~~{{STRUCTURE_2k0n\| PDB=2k0n \| SCENE= }}~~	+	A nuclear receptor-like pathway regulating multidrug resistance in fungi.,Thakur JK, Arthanari H, Yang F, Pan SJ, Fan X, Breger J, Frueh DP, Gulshan K, Li DK, Mylonakis E, Struhl K, Moye-Rowley WS, Cormack BP, Wagner G, Naar AM Nature. 2008 Apr 3;452(7187):604-9. PMID:18385733<ref>PMID:18385733</ref>

-	~~===Solution Structure~~ of ~~Yeast Gal11p kix domain===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18385733}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2k0n]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018385733<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Arthanari, H.]]		[[Category: Arthanari, H.]]

OCA: Protected "2k0n" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 14:52:29 GMT

Protected "2k0n" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:52, 7 January 2013

OCA at 14:52, 7 January 2013

OCA — Mon, 07 Jan 2013 14:52:26 GMT

←Older revision		Revision as of 14:52, 7 January 2013
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	[[Image:2k0n.png\|left\|200px]]		[[Image:2k0n.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2k0n", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
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	{{STRUCTURE_2k0n\| PDB=2k0n \| SCENE= }}		{{STRUCTURE_2k0n\| PDB=2k0n \| SCENE= }}

	===Solution Structure of Yeast Gal11p kix domain===		===Solution Structure of Yeast Gal11p kix domain===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18385733}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18385733 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_18385733}}		{{ABSTRACT_PUBMED_18385733}}

	==About this Structure==		==About this Structure==
-	~~2K0N~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA].	+	[[2k0n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18385733~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018385733</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Arthanari, H.]]		[[Category: Arthanari, H.]]
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	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 22 10:39:10 2009''

OCA at 08:39, 22 March 2009

OCA — Sun, 22 Mar 2009 08:39:11 GMT

←Older revision		Revision as of 08:39, 22 March 2009
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	===Solution Structure of Yeast Gal11p kix domain===		===Solution Structure of Yeast Gal11p kix domain===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18385733}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18385733 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18385733}}

	==About this Structure==		==About this Structure==
-	2K0N is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full ~~experimental~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA].	+	2K0N is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:18385733</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Arthanari, H.]]		[[Category: Arthanari, H.]]
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	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Feb 18 02~~:40:30 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 22 10:39:10 2009''

OCA at 00:40, 18 February 2009

OCA — Wed, 18 Feb 2009 00:40:31 GMT

←Older revision		Revision as of 00:40, 18 February 2009
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-	[[Image:2k0n.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2k0n.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2k0n\| PDB=2k0n \| SCENE= }}		{{STRUCTURE_2k0n\| PDB=2k0n \| SCENE= }}

-	~~'''~~Solution Structure of Yeast Gal11p kix domain~~'''~~	+	===Solution Structure of Yeast Gal11p kix domain===


	==About this Structure==		==About this Structure==
-	2K0N is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA].	+	2K0N is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0N OCA].
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Single protein]]
	[[Category: Arthanari, H.]]		[[Category: Arthanari, H.]]
	[[Category: Frueh, D P.]]		[[Category: Frueh, D P.]]
Line 26:		Line 26:
	[[Category: Transcription]]		[[Category: Transcription]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Apr 9 14~~:40:30 ~~2008~~''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 02:40:30 2009''