2k7x - Revision history

OCA at 09:35, 22 May 2024

OCA — Wed, 22 May 2024 09:35:59 GMT

←Older revision		Revision as of 09:35, 22 May 2024
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	==solution structure of C-terminal domain of SARS-CoV main protease==		==solution structure of C-terminal domain of SARS-CoV main protease==
-	<StructureSection load='2k7x' size='340' side='right'caption='[[2k7x~~]], [[NMR_Ensembles_of_Models \| 21 NMR models~~]]' scene=''>	+	<StructureSection load='2k7x' size='340' side='right'caption='[[2k7x]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Sars_coronavirus_sino1~~-11 ~~Sars~~ coronavirus ~~sino1~~-11]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K7X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/SARS_coronavirus_Sino1-11 SARS coronavirus Sino1-11]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K7X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [https://pdbe.org/2k7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [https://www.ebi.ac.uk/pdbsum/2k7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k7x ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [https://pdbe.org/2k7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [https://www.ebi.ac.uk/pdbsum/2k7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k7x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~Q6JH47_CVHSA Q6JH47_CVHSA~~]~~] Nsp7~~-~~nsp8 hexadecamer may possibly confer processivity~~ to the ~~polymerase~~, ~~maybe~~ by binding to ~~dsRNA or by producing primers utilized~~ by the ~~latter~~ (~~By similarity~~).[~~SAAS~~:~~SAAS008740_004_014545~~] ~~Nsp9 is~~ a ssRNA-binding protein (~~By similarity~~).~~[SAAS~~:~~SAAS008740_004_001449]~~	+	[https://www.uniprot.org/uniprot/R1AB_SARS R1AB_SARS] Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein. Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response (PubMed:23035226). May disrupt nuclear pore function by binding and displacing host NUP93 (PubMed:30943371).<ref>PMID:23035226</ref> <ref>PMID:30943371</ref> May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.<ref>PMID:19640993</ref> Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates (PubMed:17692280). Plays a role in host membrane rearrangement that leads to creation of cytoplasmic double-membrane vesicles (DMV) necessary for viral replication. Nsp3, nsp4 and nsp6 together are sufficient to form DMV (PubMed:24410069). Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3 (PubMed:19369340, PubMed:24622840). Prevents also host NF-kappa-B signaling.<ref>PMID:16271890</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> <ref>PMID:24622840</ref> <ref>PMID:24410069</ref> Plays a role in host membrane rearrangement that leads to creation of cytoplasmic double-membrane vesicles (DMV) necessary for viral replication. Alone appears incapable to induce membrane curvature, but together with nsp3 is able to induce paired membranes. Nsp3, nsp4 and nsp6 together are sufficient to form DMV.<ref>PMID:23943763</ref> <ref>PMID:24410069</ref> Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP). May cleave host ATP6V1G1 thereby modifying host vacuoles intracellular pH.[PROSITE-ProRule:PRU00772]<ref>PMID:16226257</ref> Plays a role in host membrane rearrangement that leads to creation of cytoplasmic double-membrane vesicles (DMV) necessary for viral replication. Nsp3, nsp4 and nsp6 together are sufficient to form DMV (PubMed:24410069). Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes (PubMed:24991833).<ref>PMID:24991833</ref> <ref>PMID:24410069</ref> Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.<ref>PMID:22039154</ref> Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.<ref>PMID:22039154</ref> May participate in viral replication by acting as a ssRNA-binding protein.<ref>PMID:19153232</ref> Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.<ref>PMID:22635272</ref> Responsible for replication and transcription of the viral RNA genome.<ref>PMID:22791111</ref> Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.<ref>PMID:12917423</ref> <ref>PMID:22615777</ref> Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity (PubMed:16549795, PubMed:20421945, PubMed:22635272). Acts as a proofreading exoribonuclease for RNA replication, thereby lowering The sensitivity of the virus to RNA mutagens (PubMed:23966862, PubMed:29511076, PubMed:21593585).<ref>PMID:16549795</ref> <ref>PMID:20421945</ref> <ref>PMID:21593585</ref> <ref>PMID:22635272</ref> <ref>PMID:23966862</ref> <ref>PMID:29511076</ref> Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond. Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.<ref>PMID:18417574</ref> <ref>PMID:20421945</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Sars~~ coronavirus ~~sino1~~-11]]	+	[[Category: SARS coronavirus Sino1-11]]
-	[[Category: Xia, B]]	+	[[Category: Xia B]]
-	[[Category: Zhong, N]]	+	[[Category: Zhong N]]
-	~~[[Category: C-terminal domain]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Main protease]]~~	+
-	~~[[Category: Solution structure~~]]	+

OCA at 07:32, 27 January 2022

OCA — Thu, 27 Jan 2022 07:32:21 GMT

←Older revision		Revision as of 07:32, 27 January 2022
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	<StructureSection load='2k7x' size='340' side='right'caption='[[2k7x]], [[NMR_Ensembles_of_Models \| 21 NMR models]]' scene=''>		<StructureSection load='2k7x' size='340' side='right'caption='[[2k7x]], [[NMR_Ensembles_of_Models \| 21 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Sars_coronavirus_sino1-11 Sars coronavirus sino1-11]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2K7X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sars_coronavirus_sino1-11 Sars coronavirus sino1-11]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K7X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [~~http~~://pdbe.org/2k7x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2k7x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2k7x ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [https://pdbe.org/2k7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [https://www.ebi.ac.uk/pdbsum/2k7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k7x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/Q6JH47_CVHSA Q6JH47_CVHSA]] Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity).[SAAS:SAAS008740_004_014545] Nsp9 is a ssRNA-binding protein (By similarity).[SAAS:SAAS008740_004_001449]	+	[[https://www.uniprot.org/uniprot/Q6JH47_CVHSA Q6JH47_CVHSA]] Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity).[SAAS:SAAS008740_004_014545] Nsp9 is a ssRNA-binding protein (By similarity).[SAAS:SAAS008740_004_001449]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:32, 22 April 2020

OCA — Wed, 22 Apr 2020 06:32:24 GMT

←Older revision		Revision as of 06:32, 22 April 2020
Line 1:		Line 1:

	==solution structure of C-terminal domain of SARS-CoV main protease==		==solution structure of C-terminal domain of SARS-CoV main protease==
-	<StructureSection load='2k7x' size='340' side='right' caption='[[2k7x]], [[NMR_Ensembles_of_Models \| 21 NMR models]]' scene=''>	+	<StructureSection load='2k7x' size='340' side='right'caption='[[2k7x]], [[NMR_Ensembles_of_Models \| 21 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus_sino1-11 Sars coronavirus sino1-11]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2K7X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus_sino1-11 Sars coronavirus sino1-11]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2K7X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [http://pdbe.org/2k7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [http://www.ebi.ac.uk/pdbsum/2k7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2k7x ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [http://pdbe.org/2k7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [http://www.ebi.ac.uk/pdbsum/2k7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2k7x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Sars coronavirus sino1-11]]		[[Category: Sars coronavirus sino1-11]]
	[[Category: Xia, B]]		[[Category: Xia, B]]

OCA at 11:24, 18 July 2018

OCA — Wed, 18 Jul 2018 11:24:12 GMT

←Older revision		Revision as of 11:24, 18 July 2018
Line 1:		Line 1:
		+
	==solution structure of C-terminal domain of SARS-CoV main protease==		==solution structure of C-terminal domain of SARS-CoV main protease==
	<StructureSection load='2k7x' size='340' side='right' caption='[[2k7x]], [[NMR_Ensembles_of_Models \| 21 NMR models]]' scene=''>		<StructureSection load='2k7x' size='340' side='right' caption='[[2k7x]], [[NMR_Ensembles_of_Models \| 21 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus_sino1-11 Sars coronavirus sino1-11]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K7X FirstGlance]. <br>		<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus_sino1-11 Sars coronavirus sino1-11]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K7X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [http://pdbe.org/2k7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [http://www.ebi.ac.uk/pdbsum/2k7x PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [http://pdbe.org/2k7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [http://www.ebi.ac.uk/pdbsum/2k7x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2k7x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/2k7x_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/2k7x_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:47, 9 February 2016

OCA — Tue, 09 Feb 2016 08:47:39 GMT

←Older revision		Revision as of 08:47, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k7x ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:35, 10 September 2015

OCA — Thu, 10 Sep 2015 20:35:28 GMT

←Older revision		Revision as of 20:35, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2k7x' size='340' side='right' caption='[[2k7x]], [[NMR_Ensembles_of_Models \| 21 NMR models]]' scene=''>		<StructureSection load='2k7x' size='340' side='right' caption='[[2k7x]], [[NMR_Ensembles_of_Models \| 21 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sars_coronavirus~~ Sars coronavirus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K7X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus_sino1-11 Sars coronavirus sino1-11]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K7X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [http://www.ebi.ac.uk/pdbsum/2k7x PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [http://pdbe.org/2k7x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [http://www.ebi.ac.uk/pdbsum/2k7x PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2k7x" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Sars coronavirus]]	+	[[Category: Sars coronavirus sino1-11]]
	[[Category: Xia, B]]		[[Category: Xia, B]]
	[[Category: Zhong, N]]		[[Category: Zhong, N]]

OCA at 05:49, 25 December 2014

OCA — Thu, 25 Dec 2014 05:49:16 GMT

←Older revision		Revision as of 05:49, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K7X FirstGlance]. <br>		<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K7X FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [http://www.ebi.ac.uk/pdbsum/2k7x PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [http://www.ebi.ac.uk/pdbsum/2k7x PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q6JH47_CVHSA Q6JH47_CVHSA]] Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity).[SAAS:SAAS008740_004_014545] Nsp9 is a ssRNA-binding protein (By similarity).[SAAS:SAAS008740_004_001449]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Sars coronavirus]]		[[Category: Sars coronavirus]]
-	[[Category: Xia, B.]]	+	[[Category: Xia, B]]
-	[[Category: Zhong, N.]]	+	[[Category: Zhong, N]]
	[[Category: C-terminal domain]]		[[Category: C-terminal domain]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Main protease]]		[[Category: Main protease]]
	[[Category: Solution structure]]		[[Category: Solution structure]]

OCA at 09:10, 30 September 2014

OCA — Tue, 30 Sep 2014 09:10:02 GMT

←Older revision		Revision as of 09:10, 30 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2k7x.~~png\|left~~\|200px]]	+	==solution structure of C-terminal domain of SARS-CoV main protease==
		+	<StructureSection load='2k7x' size='340' side='right' caption='[[2k7x]], [[NMR_Ensembles_of_Models \| 21 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2k7x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K7X FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k7x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k7x RCSB], [http://www.ebi.ac.uk/pdbsum/2k7x PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/2k7x_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SARS coronavirus main protease (M(pro)) plays an essential role in the extensive proteolytic processing of the viral polyproteins (pp1a and pp1ab), and it is an important target for anti-SARS drug development. We have reported that both the M(pro) C-terminal domain alone (M(pro)-C) and the N-finger deletion mutant of M(pro) (M(pro)-Delta7) exist as a stable dimer and a stable monomer (Zhong et al., J Virol 2008; 82:4227-4234). Here, we report structures of both M(pro)-C monomer and dimer. The structure of the M(pro)-C monomer is almost identical to that of the C-terminal domain in the crystal structure of M(pro). Interestingly, the M(pro)-C dimer structure is characterized by 3D domain-swapping, in which the first helices of the two protomers are interchanged and each is enwrapped by four other helices from the other protomer. Each folding subunit of the M(pro)-C domain-swapped dimer still has the same general fold as that of the M(pro)-C monomer. This special dimerization elucidates the structural basis for the observation that there is no exchange between monomeric and dimeric forms of M(pro)-C and M(pro)-Delta7.

-	~~{{STRUCTURE_2k7x\| PDB=2k7x \| SCENE= }}~~	+	C-terminal domain of SARS-CoV main protease can form a 3D domain-swapped dimer.,Zhong N, Zhang S, Xue F, Kang X, Zou P, Chen J, Liang C, Rao Z, Jin C, Lou Z, Xia B Protein Sci. 2009 Apr;18(4):839-44. PMID:19319935<ref>PMID:19319935</ref>

-	~~===solution structure~~ of ~~C-terminal domain of SARS-CoV main protease===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19319935}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2k7x]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sars_coronavirus Sars coronavirus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019319935<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Sars coronavirus]]		[[Category: Sars coronavirus]]
	[[Category: Xia, B.]]		[[Category: Xia, B.]]

OCA: Protected "2k7x" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 14:05:36 GMT

Protected "2k7x" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:05, 7 January 2013

OCA at 14:05, 7 January 2013

OCA — Mon, 07 Jan 2013 14:05:32 GMT

←Older revision		Revision as of 14:05, 7 January 2013
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:2k7x.png\|left\|200px]]
-	[[Image:2k7x.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2k7x", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2k7x\| PDB=2k7x \| SCENE= }}		{{STRUCTURE_2k7x\| PDB=2k7x \| SCENE= }}

	===solution structure of C-terminal domain of SARS-CoV main protease===		===solution structure of C-terminal domain of SARS-CoV main protease===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19319935}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19319935 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19319935}}		{{ABSTRACT_PUBMED_19319935}}

	==About this Structure==		==About this Structure==
-	~~2K7X~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA].	+	[[2k7x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K7X OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19319935~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019319935</ref><references group="xtra"/>
	[[Category: Sars coronavirus]]		[[Category: Sars coronavirus]]
	[[Category: Xia, B.]]		[[Category: Xia, B.]]
Line 31:		Line 19:
	[[Category: Main protease]]		[[Category: Main protease]]
	[[Category: Solution structure]]		[[Category: Solution structure]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 13 10:17:28 2009''