2kak - Revision history

OCA at 06:44, 1 May 2024

OCA — Wed, 01 May 2024 06:44:50 GMT

←Older revision		Revision as of 06:44, 1 May 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.		[https://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Metallothioneins (MTs) are ubiquitous cysteine-rich proteins with a high affinity for divalent metal ions such as Zn(II), Cu(I), and Cd(II) that are involved in metal ion homeostasis and detoxification, as well as protection against reactive oxygen species. Here we show the NMR solution structure of the beta(E)-domain of the early cysteine-labeled protein (E(c)-1) from wheat (beta(E)-E(c)-1), which represents the first three-dimensional structure of a plant MT. The beta(E)-domain comprises the 51 C-terminal residues of E(c)-1 and exhibits a distinctive unprecedented structure with two separate metal-binding centers, a mononuclear Zn(II) binding site constituted by two cysteine and two highly conserved histidine residues as found in certain zinc-finger motifs, and a cluster formed by three Zn(II) ions coordinated by nine Cys residues that resembles the cluster in the beta-domain of vertebrate MTs. Cys-metal ion connectivities were determined by exhaustive structure calculations for all 7560 possible configurations of the three-metal cluster. Backbone dynamics investigated by (15)N relaxation experiments support the results of the structure determination in that beta(E)-E(c)-1 is a rigidly folded polypeptide. To further investigate the influence of metal ion binding on the stability of the structure, we replaced Zn(II) with Cd(II) ions and examined the effects of metal ion release on incubation with a metal ion chelator.
-
-	The beta(E)-domain of wheat E(c)-1 metallothionein: a metal-binding domain with a distinctive structure.,Peroza EA, Schmucki R, Guntert P, Freisinger E, Zerbe O J Mol Biol. 2009 Mar 20;387(1):207-18. Epub 2009 Jan 27. PMID:19361445<ref>PMID:19361445</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2kak" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:36, 30 August 2023

OCA — Wed, 30 Aug 2023 13:36:21 GMT

←Older revision		Revision as of 13:36, 30 August 2023
Line 1:		Line 1:

	==Solution structure of the beta-E-domain of wheat Ec-1 metallothionein==		==Solution structure of the beta-E-domain of wheat Ec-1 metallothionein==
-	<StructureSection load='2kak' size='340' side='right'caption='[[2kak~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2kak' size='340' side='right'caption='[[2kak]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Canadian_hard_winter_wheat Canadian hard winter wheat~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KAK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KAK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kak OCA], [https://pdbe.org/2kak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kak RCSB], [https://www.ebi.ac.uk/pdbsum/2kak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kak ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kak OCA], [https://pdbe.org/2kak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kak RCSB], [https://www.ebi.ac.uk/pdbsum/2kak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kak ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT]] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.	+	[https://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Canadian hard winter wheat]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Freisinger, E]]	+	[[Category: Triticum aestivum]]
-	[[Category: Guntert, P]]	+	[[Category: Freisinger E]]
-	[[Category: Peroza~~, E A~~]]	+	[[Category: Guntert P]]
-	[[Category: Schmucki, R]]	+	[[Category: Peroza EA]]
-	[[Category: Zerbe, O]]	+	[[Category: Schmucki R]]
-	~~[[Category: Metal binding protein]]~~	+	[[Category: Zerbe O]]
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Metal-thiolate cluster]]~~	+
-	~~[[Category: Metallothionein]]~~	+
-	~~[[Category: Solution structure]]~~	+
-	~~[[Category: Wheat ec-1]]~~	+
-	~~[[Category: Zinc]]~~	+
-	~~[[Category: Zn binding~~]]	+

OCA at 13:30, 24 November 2021

OCA — Wed, 24 Nov 2021 13:30:27 GMT

←Older revision		Revision as of 13:30, 24 November 2021
Line 3:		Line 3:
	<StructureSection load='2kak' size='340' side='right'caption='[[2kak]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kak' size='340' side='right'caption='[[2kak]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kak]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2KAK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KAK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2kak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kak OCA], [~~http~~://pdbe.org/2kak PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2kak RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2kak PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2kak ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kak OCA], [https://pdbe.org/2kak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kak RCSB], [https://www.ebi.ac.uk/pdbsum/2kak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kak ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT]] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.	+	[[https://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT]] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 2kak" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2kak" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Journal:JBIC:9\|Journal:JBIC:9]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:57, 19 February 2020

OCA — Wed, 19 Feb 2020 06:57:55 GMT

←Older revision		Revision as of 06:57, 19 February 2020
Line 1:		Line 1:

	==Solution structure of the beta-E-domain of wheat Ec-1 metallothionein==		==Solution structure of the beta-E-domain of wheat Ec-1 metallothionein==
-	<StructureSection load='2kak' size='340' side='right' caption='[[2kak]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2kak' size='340' side='right'caption='[[2kak]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KAK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KAK FirstGlance]. <br>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Canadian hard winter wheat]]		[[Category: Canadian hard winter wheat]]
		+	[[Category: Large Structures]]
	[[Category: Freisinger, E]]		[[Category: Freisinger, E]]
	[[Category: Guntert, P]]		[[Category: Guntert, P]]

OCA at 10:23, 18 July 2018

OCA — Wed, 18 Jul 2018 10:23:56 GMT

←Older revision		Revision as of 10:23, 18 July 2018
Line 1:		Line 1:
		+
	==Solution structure of the beta-E-domain of wheat Ec-1 metallothionein==		==Solution structure of the beta-E-domain of wheat Ec-1 metallothionein==
	<StructureSection load='2kak' size='340' side='right' caption='[[2kak]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kak' size='340' side='right' caption='[[2kak]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2kak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KAK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KAK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kak OCA], [http://pdbe.org/2kak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kak RCSB], [http://www.ebi.ac.uk/pdbsum/2kak PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kak OCA], [http://pdbe.org/2kak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kak RCSB], [http://www.ebi.ac.uk/pdbsum/2kak PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kak ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 36:		Line 37:
	[[Category: Solution structure]]		[[Category: Solution structure]]
	[[Category: Wheat ec-1]]		[[Category: Wheat ec-1]]
		+	[[Category: Zinc]]
	[[Category: Zn binding]]		[[Category: Zn binding]]

OCA at 06:56, 10 September 2015

OCA — Thu, 10 Sep 2015 06:56:53 GMT

←Older revision		Revision as of 06:56, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2kak' size='340' side='right' caption='[[2kak]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kak' size='340' side='right' caption='[[2kak]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Triticum_aestivum Triticum aestivum~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KAK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KAK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kak RCSB], [http://www.ebi.ac.uk/pdbsum/2kak PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kak OCA], [http://pdbe.org/2kak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kak RCSB], [http://www.ebi.ac.uk/pdbsum/2kak PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2kak" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Triticum aestivum~~]]	+	[[Category: Canadian hard winter wheat]]
	[[Category: Freisinger, E]]		[[Category: Freisinger, E]]
	[[Category: Guntert, P]]		[[Category: Guntert, P]]

OCA at 19:51, 24 December 2014

OCA — Wed, 24 Dec 2014 19:51:08 GMT

←Older revision		Revision as of 19:51, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kak RCSB], [http://www.ebi.ac.uk/pdbsum/2kak PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kak RCSB], [http://www.ebi.ac.uk/pdbsum/2kak PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT]] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 14:01, 17 December 2014

OCA — Wed, 17 Dec 2014 14:01:29 GMT

←Older revision		Revision as of 14:01, 17 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2kak\| PDB=2kak \| SCENE= }}~~	+	==Solution structure of the beta-E-domain of wheat Ec-1 metallothionein==
-	===Solution structure of the beta-E-domain of wheat Ec-1 metallothionein===	+	<StructureSection load='2kak' size='340' side='right' caption='[[2kak]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~{{ABSTRACT_PUBMED_19361445}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2kak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KAK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kak RCSB], [http://www.ebi.ac.uk/pdbsum/2kak PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metallothioneins (MTs) are ubiquitous cysteine-rich proteins with a high affinity for divalent metal ions such as Zn(II), Cu(I), and Cd(II) that are involved in metal ion homeostasis and detoxification, as well as protection against reactive oxygen species. Here we show the NMR solution structure of the beta(E)-domain of the early cysteine-labeled protein (E(c)-1) from wheat (beta(E)-E(c)-1), which represents the first three-dimensional structure of a plant MT. The beta(E)-domain comprises the 51 C-terminal residues of E(c)-1 and exhibits a distinctive unprecedented structure with two separate metal-binding centers, a mononuclear Zn(II) binding site constituted by two cysteine and two highly conserved histidine residues as found in certain zinc-finger motifs, and a cluster formed by three Zn(II) ions coordinated by nine Cys residues that resembles the cluster in the beta-domain of vertebrate MTs. Cys-metal ion connectivities were determined by exhaustive structure calculations for all 7560 possible configurations of the three-metal cluster. Backbone dynamics investigated by (15)N relaxation experiments support the results of the structure determination in that beta(E)-E(c)-1 is a rigidly folded polypeptide. To further investigate the influence of metal ion binding on the stability of the structure, we replaced Zn(II) with Cd(II) ions and examined the effects of metal ion release on incubation with a metal ion chelator.

-	~~==About this Structure==~~	+	The beta(E)-domain of wheat E(c)-1 metallothionein: a metal-binding domain with a distinctive structure.,Peroza EA, Schmucki R, Guntert P, Freisinger E, Zerbe O J Mol Biol. 2009 Mar 20;387(1):207-18. Epub 2009 Jan 27. PMID:19361445<ref>PMID:19361445</ref>
-	~~[[2kak]] is a~~ 1 ~~chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Triticum_aestivum Triticum aestivum]~~. ~~Full experimental information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=2KAK OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Journal:JBIC:9\|Journal:JBIC:9]]		*[[Journal:JBIC:9\|Journal:JBIC:9]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019361445<~~/~~ref~~><~~ref group="xtra">DOI 10.1007/s00775-011-0770-2</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Triticum aestivum]]		[[Category: Triticum aestivum]]
-	[[Category: Freisinger, E.]]	+	[[Category: Freisinger, E]]
-	[[Category: Guntert, P.]]	+	[[Category: Guntert, P]]
-	[[Category: Peroza, E A.]]	+	[[Category: Peroza, E A]]
-	[[Category: Schmucki, R.]]	+	[[Category: Schmucki, R]]
-	[[Category: Zerbe, O.]]	+	[[Category: Zerbe, O]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]

OCA at 09:14, 11 March 2013

OCA — Mon, 11 Mar 2013 09:14:10 GMT

←Older revision		Revision as of 09:14, 11 March 2013
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-	[[Image:2kak.png\|left\|200px]]
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	{{STRUCTURE_2kak\| PDB=2kak \| SCENE= }}		{{STRUCTURE_2kak\| PDB=2kak \| SCENE= }}
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	===Solution structure of the beta-E-domain of wheat Ec-1 metallothionein===		===Solution structure of the beta-E-domain of wheat Ec-1 metallothionein===
-
	{{ABSTRACT_PUBMED_19361445}}		{{ABSTRACT_PUBMED_19361445}}

OCA: Protected "2kak" [edit=sysop:move=sysop]

OCA — Wed, 29 Aug 2012 04:01:36 GMT

Protected "2kak" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:01, 29 August 2012