2kfs - Revision history

OCA at 09:47, 9 May 2024

OCA — Thu, 09 May 2024 09:47:16 GMT

←Older revision		Revision as of 09:47, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KFS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KFS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [https://pdbe.org/2kfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [https://www.ebi.ac.uk/pdbsum/2kfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfs ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [https://pdbe.org/2kfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [https://www.ebi.ac.uk/pdbsum/2kfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:44, 14 June 2023

OCA — Wed, 14 Jun 2023 08:44:21 GMT

←Older revision		Revision as of 08:44, 14 June 2023
Line 1:		Line 1:

	==NMR structure of Rv2175c==		==NMR structure of Rv2175c==
-	<StructureSection load='2kfs' size='340' side='right'caption='[[2kfs~~]], [[NMR_Ensembles_of_Models \| 30 NMR models~~]]' scene=''>	+	<StructureSection load='2kfs' size='340' side='right'caption='[[2kfs]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KFS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KFS FirstGlance]. <br>
-	</td></tr>~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2175c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [https://pdbe.org/2kfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [https://www.ebi.ac.uk/pdbsum/2kfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfs ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [https://pdbe.org/2kfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [https://www.ebi.ac.uk/pdbsum/2kfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfs ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/Y2175_MYCTU Y2175_MYCTU]] Binds DNA at low salt concentrations.<ref>PMID:19457863</ref>	+	[https://www.uniprot.org/uniprot/Y2175_MYCTU Y2175_MYCTU] Binds DNA at low salt concentrations.<ref>PMID:19457863</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Barthe, P]]	+	[[Category: Barthe P]]
-	[[Category: Cohen-Gonsaud, M]]	+	[[Category: Cohen-Gonsaud M]]
-	[[Category: Molle, V]]	+	[[Category: Molle V]]
-	[[Category: Roumestand, C]]	+	[[Category: Roumestand C]]
-	~~[[Category: Dna binding]]~~	+
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Dna-binding protein]]~~	+
-	~~[[Category: Phosphorylation]]~~	+
-	~~[[Category: Whth~~]]	+

OCA at 07:04, 1 December 2021

OCA — Wed, 01 Dec 2021 07:04:42 GMT

←Older revision		Revision as of 07:04, 1 December 2021
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	<StructureSection load='2kfs' size='340' side='right'caption='[[2kfs]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>		<StructureSection load='2kfs' size='340' side='right'caption='[[2kfs]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Myctu Myctu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2KFS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KFS FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2175c ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2175c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [~~http~~://pdbe.org/2kfs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2kfs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [https://pdbe.org/2kfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [https://www.ebi.ac.uk/pdbsum/2kfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/Y2175_MYCTU Y2175_MYCTU]] Binds DNA at low salt concentrations.<ref>PMID:19457863</ref>	+	[[https://www.uniprot.org/uniprot/Y2175_MYCTU Y2175_MYCTU]] Binds DNA at low salt concentrations.<ref>PMID:19457863</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:34, 26 February 2020

OCA — Wed, 26 Feb 2020 09:34:47 GMT

←Older revision		Revision as of 09:34, 26 February 2020
Line 1:		Line 1:

	==NMR structure of Rv2175c==		==NMR structure of Rv2175c==
-	<StructureSection load='2kfs' size='340' side='right' caption='[[2kfs]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>	+	<StructureSection load='2kfs' size='340' side='right'caption='[[2kfs]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KFS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KFS FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Myctu]]		[[Category: Myctu]]
	[[Category: Barthe, P]]		[[Category: Barthe, P]]

OCA at 11:07, 18 July 2018

OCA — Wed, 18 Jul 2018 11:07:47 GMT

←Older revision		Revision as of 11:07, 18 July 2018
Line 1:		Line 1:
		+
	==NMR structure of Rv2175c==		==NMR structure of Rv2175c==
	<StructureSection load='2kfs' size='340' side='right' caption='[[2kfs]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>		<StructureSection load='2kfs' size='340' side='right' caption='[[2kfs]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KFS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KFS FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2175c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2175c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [http://pdbe.org/2kfs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [http://www.ebi.ac.uk/pdbsum/2kfs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [http://pdbe.org/2kfs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [http://www.ebi.ac.uk/pdbsum/2kfs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kf/2kfs_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kf/2kfs_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kfs ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	[[Category: Roumestand, C]]		[[Category: Roumestand, C]]
	[[Category: Dna binding]]		[[Category: Dna binding]]
		+	[[Category: Dna binding protein]]
	[[Category: Dna-binding protein]]		[[Category: Dna-binding protein]]
	[[Category: Phosphorylation]]		[[Category: Phosphorylation]]
	[[Category: Whth]]		[[Category: Whth]]

OCA at 17:04, 11 September 2015

OCA — Fri, 11 Sep 2015 17:04:29 GMT

←Older revision		Revision as of 17:04, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2kfs' size='340' side='right' caption='[[2kfs]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>		<StructureSection load='2kfs' size='340' side='right' caption='[[2kfs]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis Mycobacterium tuberculosis~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KFS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KFS FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2175c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1773 Mycobacterium tuberculosis~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2175c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [http://www.ebi.ac.uk/pdbsum/2kfs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [http://pdbe.org/2kfs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [http://www.ebi.ac.uk/pdbsum/2kfs PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~O53509_MYCTU O53509_MYCTU~~]] Binds DNA at low salt concentrations.<ref>PMID:19457863</ref>	+	[[http://www.uniprot.org/uniprot/Y2175_MYCTU Y2175_MYCTU]] Binds DNA at low salt concentrations.<ref>PMID:19457863</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2kfs" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mycobacterium tuberculosis~~]]	+	[[Category: Myctu]]
	[[Category: Barthe, P]]		[[Category: Barthe, P]]
	[[Category: Cohen-Gonsaud, M]]		[[Category: Cohen-Gonsaud, M]]

OCA at 19:22, 25 December 2014

OCA — Thu, 25 Dec 2014 19:22:49 GMT

←Older revision		Revision as of 19:22, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KFS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KFS FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2175c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2175c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [http://www.ebi.ac.uk/pdbsum/2kfs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [http://www.ebi.ac.uk/pdbsum/2kfs PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/O53509_MYCTU O53509_MYCTU]] Binds DNA at low salt concentrations.<ref>PMID:19457863</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
-	[[Category: Barthe, P.]]	+	[[Category: Barthe, P]]
-	[[Category: Cohen-Gonsaud, M.]]	+	[[Category: Cohen-Gonsaud, M]]
-	[[Category: Molle, V.]]	+	[[Category: Molle, V]]
-	[[Category: Roumestand, C.]]	+	[[Category: Roumestand, C]]
	[[Category: Dna binding]]		[[Category: Dna binding]]
	[[Category: Dna-binding protein]]		[[Category: Dna-binding protein]]
	[[Category: Phosphorylation]]		[[Category: Phosphorylation]]
	[[Category: Whth]]		[[Category: Whth]]

OCA at 08:13, 30 September 2014

OCA — Tue, 30 Sep 2014 08:13:49 GMT

←Older revision		Revision as of 08:13, 30 September 2014
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-	[[~~Image~~:2kfs.~~png\|left~~\|200px]]	+	==NMR structure of Rv2175c==
		+	<StructureSection load='2kfs' size='340' side='right' caption='[[2kfs]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KFS FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv2175c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [http://www.ebi.ac.uk/pdbsum/2kfs PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kf/2kfs_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Recent efforts have underlined the role of serine/threonine protein kinases in growth, pathogenesis, and cell wall metabolism in Mycobacterium tuberculosis. Although most kinases have been investigated for their physiological roles, little information is available regarding how serine/threonine protein kinase-dependent phosphorylation regulates the activity of kinase substrates. Herein, we focused on M. tuberculosis Rv2175c, a protein of unknown function, conserved in actinomycetes, and recently identified as a substrate of the PknL kinase. We solved the solution structure of Rv2175c by multidimensional NMR and demonstrated that it possesses an original winged helix-turn-helix motif, indicative of a DNA-binding protein. The DNA-binding activity of Rv2175c was subsequently confirmed by fluorescence anisotropy, as well as in electrophoretic mobility shift assays. Mass spectrometry analyses using a combination of MALDI-TOF and LC-ESI/MS/MS identified Thr(9) as the unique phosphoacceptor. This was further supported by complete loss of PknL-dependent phosphorylation of an Rv2175c_T9A mutant. Importantly, the DNA-binding activity was completely abrogated in a Rv2175c_T9D mutant, designed to mimic constitutive phosphorylation, but not in a mutant lacking the first 13 residues. This implies that the function of the N-terminal extension is to provide a phosphoacceptor (Thr(9)), which, following phosphorylation, negatively regulates the Rv2175c DNA-binding activity. Interestingly, the N-terminal disordered extension, which bears the phosphoacceptor, was found to be restricted to members of the M. tuberculosis complex, thus suggesting the existence of an original mechanism that appears to be unique to the M. tuberculosis complex.

-	~~{{STRUCTURE_2kfs\| PDB=2kfs \| SCENE= }}~~	+	The Mycobacterium tuberculosis Ser/Thr kinase substrate Rv2175c is a DNA-binding protein regulated by phosphorylation.,Cohen-Gonsaud M, Barthe P, Canova MJ, Stagier-Simon C, Kremer L, Roumestand C, Molle V J Biol Chem. 2009 Jul 17;284(29):19290-300. Epub 2009 May 20. PMID:19457863<ref>PMID:19457863</ref>

-	~~===NMR structure~~ of ~~Rv2175c===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19457863}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2kfs]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019457863<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Barthe, P.]]		[[Category: Barthe, P.]]

OCA: Protected "2kfs" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 10:40:37 GMT

Protected "2kfs" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:40, 7 January 2013

OCA at 10:40, 7 January 2013

OCA — Mon, 07 Jan 2013 10:40:33 GMT

←Older revision		Revision as of 10:40, 7 January 2013
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-	{{Seed}}
	[[Image:2kfs.png\|left\|200px]]		[[Image:2kfs.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2kfs", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
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	{{STRUCTURE_2kfs\| PDB=2kfs \| SCENE= }}		{{STRUCTURE_2kfs\| PDB=2kfs \| SCENE= }}

	===NMR structure of Rv2175c===		===NMR structure of Rv2175c===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19457863}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19457863 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_19457863}}		{{ABSTRACT_PUBMED_19457863}}

	==About this Structure==		==About this Structure==
-	~~2KFS~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA].	+	[[2kfs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19457863~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019457863</ref><references group="xtra"/>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Barthe, P.]]		[[Category: Barthe, P.]]
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	[[Category: Phosphorylation]]		[[Category: Phosphorylation]]
	[[Category: Whth]]		[[Category: Whth]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jan 18 10:11:18 2010''