2kom - Revision history

OCA at 09:40, 22 May 2024

OCA — Wed, 22 May 2024 09:40:53 GMT

←Older revision		Revision as of 09:40, 22 May 2024
Line 1:		Line 1:

	==Solution structure of humar Par-3b PDZ2 (residues 451-549)==		==Solution structure of humar Par-3b PDZ2 (residues 451-549)==
-	<StructureSection load='2kom' size='340' side='right'caption='[[2kom~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2kom' size='340' side='right'caption='[[2kom]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kom]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KOM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kom]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KOM FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>PARD3, PAR3, PAR3A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kom OCA], [https://pdbe.org/2kom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kom RCSB], [https://www.ebi.ac.uk/pdbsum/2kom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kom ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kom OCA], [https://pdbe.org/2kom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kom RCSB], [https://www.ebi.ac.uk/pdbsum/2kom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kom ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PARD3_HUMAN PARD3_HUMAN]] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.<ref>PMID:19812038</ref>	+	[https://www.uniprot.org/uniprot/PARD3_HUMAN PARD3_HUMAN] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.<ref>PMID:19812038</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Structural genomic]]~~	+	[[Category: Peterson FC]]
-	[[Category: Peterson~~, F C~~]]	+	[[Category: Tyler RC]]
-	[[Category: Tyler~~, R C~~]]	+	[[Category: Volkman BF]]
-	[[Category: Volkman~~, B F]]~~	+
-	~~[[Category: Alternative splicing]]~~	+
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Cell division]]~~	+
-	~~[[Category: Cell junction]]~~	+
-	~~[[Category: Cell membrane]]~~	+
-	~~[[Category: Cesg]]~~	+
-	~~[[Category: Coiled coil]]~~	+
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Cytoskeleton]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Par-3b]]~~	+
-	~~[[Category: Pdz domain]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Polymorphism]]~~	+
-	~~[[Category: PSI, Protein structure initiative]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Tight junction~~]]	+

OCA at 07:29, 14 April 2021

OCA — Wed, 14 Apr 2021 07:29:37 GMT

←Older revision		Revision as of 07:29, 14 April 2021
Line 1:		Line 1:

	==Solution structure of humar Par-3b PDZ2 (residues 451-549)==		==Solution structure of humar Par-3b PDZ2 (residues 451-549)==
-	<StructureSection load='2kom' size='340' side='right' caption='[[2kom]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2kom' size='340' side='right'caption='[[2kom]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kom]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2KOM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kom]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KOM FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PARD3, PAR3, PAR3A ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PARD3, PAR3, PAR3A ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2kom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kom OCA], [~~http~~://pdbe.org/2kom PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2kom RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2kom PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2kom ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kom OCA], [https://pdbe.org/2kom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kom RCSB], [https://www.ebi.ac.uk/pdbsum/2kom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kom ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PARD3_HUMAN PARD3_HUMAN]] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.<ref>PMID:19812038</ref>	+	[[https://www.uniprot.org/uniprot/PARD3_HUMAN PARD3_HUMAN]] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.<ref>PMID:19812038</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Peterson, F C]]		[[Category: Peterson, F C]]

OCA at 08:01, 25 July 2018

OCA — Wed, 25 Jul 2018 08:01:02 GMT

←Older revision		Revision as of 08:01, 25 July 2018
Line 1:		Line 1:
		+
	==Solution structure of humar Par-3b PDZ2 (residues 451-549)==		==Solution structure of humar Par-3b PDZ2 (residues 451-549)==
	<StructureSection load='2kom' size='340' side='right' caption='[[2kom]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kom' size='340' side='right' caption='[[2kom]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2kom]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOM FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kom]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOM FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PARD3, PAR3, PAR3A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PARD3, PAR3, PAR3A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kom OCA], [http://pdbe.org/2kom PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kom RCSB], [http://www.ebi.ac.uk/pdbsum/2kom PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kom OCA], [http://pdbe.org/2kom PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kom RCSB], [http://www.ebi.ac.uk/pdbsum/2kom PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kom ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/2kom_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/2kom_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 36:		Line 37:
	[[Category: Tyler, R C]]		[[Category: Tyler, R C]]
	[[Category: Volkman, B F]]		[[Category: Volkman, B F]]
		+	[[Category: Alternative splicing]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Cell division]]		[[Category: Cell division]]
Line 41:		Line 43:
	[[Category: Cell membrane]]		[[Category: Cell membrane]]
	[[Category: Cesg]]		[[Category: Cesg]]
		+	[[Category: Coiled coil]]
		+	[[Category: Cytoplasm]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
	[[Category: Membrane]]		[[Category: Membrane]]
Line 46:		Line 50:
	[[Category: Pdz domain]]		[[Category: Pdz domain]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
		+	[[Category: Polymorphism]]
	[[Category: PSI, Protein structure initiative]]		[[Category: PSI, Protein structure initiative]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]
	[[Category: Tight junction]]		[[Category: Tight junction]]

OCA at 18:12, 8 February 2016

OCA — Mon, 08 Feb 2016 18:12:36 GMT

←Older revision		Revision as of 18:12, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kom ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:08, 10 September 2015

OCA — Thu, 10 Sep 2015 14:08:22 GMT

←Older revision		Revision as of 14:08, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2kom' size='340' side='right' caption='[[2kom]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kom' size='340' side='right' caption='[[2kom]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kom]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kom]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOM FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PARD3, PAR3, PAR3A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PARD3, PAR3, PAR3A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kom OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kom RCSB], [http://www.ebi.ac.uk/pdbsum/2kom PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kom OCA], [http://pdbe.org/2kom PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kom RCSB], [http://www.ebi.ac.uk/pdbsum/2kom PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2kom" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Peterson, F C]]		[[Category: Peterson, F C]]

OCA at 01:13, 25 December 2014

OCA — Thu, 25 Dec 2014 01:13:53 GMT

←Older revision		Revision as of 01:13, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kom]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOM FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kom]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOM FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PARD3, PAR3, PAR3A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PARD3, PAR3, PAR3A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kom OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kom RCSB], [http://www.ebi.ac.uk/pdbsum/2kom PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kom OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kom RCSB], [http://www.ebi.ac.uk/pdbsum/2kom PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PARD3_HUMAN PARD3_HUMAN]] Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.<ref>PMID:19812038</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: ~~CESG, Center for Eukaryotic~~ Structural ~~Genomics.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Peterson, F C.]]	+	[[Category: Peterson, F C]]
-	[[Category: Tyler, R C.]]	+	[[Category: Tyler, R C]]
-	[[Category: Volkman, B F.]]	+	[[Category: Volkman, B F]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Cell division]]		[[Category: Cell division]]
	[[Category: Cell junction]]		[[Category: Cell junction]]
	[[Category: Cell membrane]]		[[Category: Cell membrane]]
-	[[Category: Center for eukaryotic structural genomic]]
	[[Category: Cesg]]		[[Category: Cesg]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
Line 44:		Line 45:
	[[Category: Pdz domain]]		[[Category: Pdz domain]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
-	[[Category: Protein structure initiative]]	+	[[Category: PSI, Protein structure initiative]]
-	~~[[Category: Psi~~]]	+
	[[Category: Signaling protein]]		[[Category: Signaling protein]]
-	[[Category: Structural genomic]]
	[[Category: Tight junction]]		[[Category: Tight junction]]

OCA at 08:37, 30 September 2014

OCA — Tue, 30 Sep 2014 08:37:44 GMT

←Older revision		Revision as of 08:37, 30 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2kom.~~png\|left~~\|200px]]	+	==Solution structure of humar Par-3b PDZ2 (residues 451-549)==
		+	<StructureSection load='2kom' size='340' side='right' caption='[[2kom]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2kom]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KOM FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PARD3, PAR3, PAR3A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kom OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kom RCSB], [http://www.ebi.ac.uk/pdbsum/2kom PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/2kom_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Three-dimensional protein structure determination is a costly process due in part to the low success rate within groups of potential targets. Conventional validation methods eliminate the vast majority of proteins from further consideration through a time-consuming succession of screens for expression, solubility, purification, and folding. False negatives at each stage incur unwarranted reductions in the overall success rate. We developed a semi-automated protocol for isotopically-labeled protein production using the Maxwell-16, a commercially available bench top robot, that allows for single-step target screening by 2D NMR. In the span of a week, one person can express, purify, and screen 48 different (15)N-labeled proteins, accelerating the validation process by more than 10-fold. The yield from a single channel of the Maxwell-16 is sufficient for acquisition of a high-quality 2D (1)H-(15)N-HSQC spectrum using a 3-mm sample cell and 5-mm cryogenic NMR probe. Maxwell-16 screening of a control group of proteins reproduced previous validation results from conventional small-scale expression screening and large-scale production approaches currently employed by our structural genomics pipeline. Analysis of 18 new protein constructs identified two potential structure targets that included the second PDZ domain of human Par-3. To further demonstrate the broad utility of this production strategy, we solved the PDZ2 NMR structure using [U-(15)N,(13)C] protein prepared using the Maxwell-16. This novel semi-automated protein production protocol reduces the time and cost associated with NMR structure determination by eliminating unnecessary screening and scale-up steps.

-	~~{{STRUCTURE_2kom\| PDB=2kom \| SCENE= }}~~	+	Rapid, robotic, small-scale protein production for NMR screening and structure determination.,Jensen DR, Woytovich C, Li M, Duvnjak P, Cassidy MS, Frederick RO, Bergeman LF, Peterson FC, Volkman BF Protein Sci. 2010 Mar;19(3):570-8. PMID:20073081<ref>PMID:20073081</ref>

-	~~===Solution structure~~ of ~~humar Par-3b PDZ2 (residues 451-549)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20073081}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2kom]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:020073081<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: CESG, Center for Eukaryotic Structural Genomics.]]		[[Category: CESG, Center for Eukaryotic Structural Genomics.]]

OCA: Protected "2kom" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 13:37:12 GMT

Protected "2kom" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:37, 7 January 2013

OCA at 13:37, 7 January 2013

OCA — Mon, 07 Jan 2013 13:37:08 GMT

←Older revision		Revision as of 13:37, 7 January 2013
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-	{{Seed}}
	[[Image:2kom.png\|left\|200px]]		[[Image:2kom.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2kom\| PDB=2kom \| SCENE= }}		{{STRUCTURE_2kom\| PDB=2kom \| SCENE= }}

	===Solution structure of humar Par-3b PDZ2 (residues 451-549)===		===Solution structure of humar Par-3b PDZ2 (residues 451-549)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20073081}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_20073081}}		{{ABSTRACT_PUBMED_20073081}}

	==About this Structure==		==About this Structure==
-	~~2KOM~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA].	+	[[2kom]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20073081~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020073081</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: CESG, Center for Eukaryotic Structural Genomics.]]		[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
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	[[Category: Tyler, R C.]]		[[Category: Tyler, R C.]]
	[[Category: Volkman, B F.]]		[[Category: Volkman, B F.]]
-	[[Category: Alternative splicing]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Cell division]]		[[Category: Cell division]]
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	[[Category: Center for eukaryotic structural genomic]]		[[Category: Center for eukaryotic structural genomic]]
	[[Category: Cesg]]		[[Category: Cesg]]
-	[[Category: Coiled coil]]
-	[[Category: Cytoplasm]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
	[[Category: Membrane]]		[[Category: Membrane]]
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	[[Category: Pdz domain]]		[[Category: Pdz domain]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
-	[[Category: Polymorphism]]
	[[Category: Protein structure initiative]]		[[Category: Protein structure initiative]]
	[[Category: Psi]]		[[Category: Psi]]
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	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Tight junction]]		[[Category: Tight junction]]
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-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 17 08:55:27 2010''

OCA at 06:55, 17 March 2010

OCA — Wed, 17 Mar 2010 06:55:28 GMT

←Older revision		Revision as of 06:55, 17 March 2010
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	{{Seed}}		{{Seed}}
-	[[Image:2kom.~~jpg~~\|left\|200px]]	+	[[Image:2kom.png\|left\|200px]]

	<!--		<!--
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	===Solution structure of humar Par-3b PDZ2 (residues 451-549)===		===Solution structure of humar Par-3b PDZ2 (residues 451-549)===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20073081}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20073081 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20073081}}

	==About this Structure==		==About this Structure==
-	2KOM is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA].	+	2KOM is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOM OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20073081</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: CESG, Center for Eukaryotic Structural Genomics.]]		[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
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	[[Category: Tight junction]]		[[Category: Tight junction]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Nov 11 22~~:13:~~00 2009~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 17 08:55:27 2010''