2ks4 - Revision history

OCA at 06:48, 1 May 2024

OCA — Wed, 01 May 2024 06:48:18 GMT

←Older revision		Revision as of 06:48, 1 May 2024
Line 1:		Line 1:

	==NMR structure of the sea anemone actinoporin Sticholysin==		==NMR structure of the sea anemone actinoporin Sticholysin==
-	<StructureSection load='2ks4' size='340' side='right'caption='[[2ks4~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2ks4' size='340' side='right'caption='[[2ks4]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ks4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Stihl Stihl~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KS4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ks4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KS4 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2ks3\|2ks3]], [[1kd6\|1kd6]], [[1iaz\|1iaz]], [[1gwy\|1gwy]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STCH1, STCH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6123 STIHL])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [https://pdbe.org/2ks4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ks4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ks4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [https://pdbe.org/2ks4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ks4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ks4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~ACTP1_STOHE ACTP1_STOHE]~~] Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and ~~hemolysis~~. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of ~~several~~ monomers. Cytolytic effects include red blood cells hemolysis, platelet aggregation and lysis, cytotoxic and cytostatic effects on fibroblasts. Lethality in mammals has been ascribed to severe vasospasm of coronary vessels, cardiac arrhythmia, and inotropic effects ~~(By similarity)~~.	+	[https://www.uniprot.org/uniprot/ACTP1_STIHL ACTP1_STIHL] Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and cytolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of monomers. Cytolytic effects include red blood cells hemolysis, platelet aggregation and lysis, cytotoxic and cytostatic effects on fibroblasts. Lethality in mammals has been ascribed to severe vasospasm of coronary vessels, cardiac arrhythmia, and inotropic effects.<ref>PMID:10978735</ref> <ref>PMID:11478962</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ks4 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ks4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Sticholysin I is an actinoporin, a pore forming toxin, of 176 aminoacids produced by the sea anemone Stichodactyla heliantus. Isotopically labelled (13)C/(15)N recombinant protein was produced in E. coli. Here we report the complete NMR (15)N, (13)C and (1)H chemical shifts assignments of Stn I at pH 4.0 and 25 degrees C (BMRB No. 15927).
-
-	1H, 13C, and 15N NMR assignments of the actinoporin Sticholysin I.,Castrillo I, Alegre-Cebollada J, del Pozo AM, Gavilanes JG, Santoro J, Bruix M Biomol NMR Assign. 2009 Jun;3(1):5-7. Epub 2008 Nov 6. PMID:19636934<ref>PMID:19636934</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2ks4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Stihl~~]]	+	[[Category: Stichodactyla helianthus]]
-	[[Category: Bruix, M]]	+	[[Category: Bruix M]]
-	[[Category: Castrillo, I]]	+	[[Category: Castrillo I]]
-	[[Category: Santoro, J]]	+	[[Category: Santoro J]]
-	~~[[Category: Cytolysis]]~~	+
-	~~[[Category: Hemolysis]]~~	+
-	~~[[Category: Ion transport]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Nematocyst]]~~	+
-	~~[[Category: Porin]]~~	+
-	~~[[Category: Protein]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Toxin]]~~	+
-	~~[[Category: Transmembrane]]~~	+
-	~~[[Category: Transport]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 07:31, 14 April 2021

OCA — Wed, 14 Apr 2021 07:31:09 GMT

←Older revision		Revision as of 07:31, 14 April 2021
Line 1:		Line 1:

	==NMR structure of the sea anemone actinoporin Sticholysin==		==NMR structure of the sea anemone actinoporin Sticholysin==
-	<StructureSection load='2ks4' size='340' side='right' caption='[[2ks4]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2ks4' size='340' side='right'caption='[[2ks4]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ks4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Stihl Stihl]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2KS4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ks4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stihl Stihl]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KS4 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ks3\|2ks3]], [[1kd6\|1kd6]], [[1iaz\|1iaz]], [[1gwy\|1gwy]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ks3\|2ks3]], [[1kd6\|1kd6]], [[1iaz\|1iaz]], [[1gwy\|1gwy]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STCH1, STCH ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6123 STIHL])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STCH1, STCH ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6123 STIHL])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [~~http~~://pdbe.org/2ks4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ks4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ks4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [https://pdbe.org/2ks4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [https://www.ebi.ac.uk/pdbsum/2ks4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ks4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ACTP1_STOHE ACTP1_STOHE]] Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and hemolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of several monomers. Cytolytic effects include red blood cells hemolysis, platelet aggregation and lysis, cytotoxic and cytostatic effects on fibroblasts. Lethality in mammals has been ascribed to severe vasospasm of coronary vessels, cardiac arrhythmia, and inotropic effects (By similarity).	+	[[https://www.uniprot.org/uniprot/ACTP1_STOHE ACTP1_STOHE]] Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and hemolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of several monomers. Cytolytic effects include red blood cells hemolysis, platelet aggregation and lysis, cytotoxic and cytostatic effects on fibroblasts. Lethality in mammals has been ascribed to severe vasospasm of coronary vessels, cardiac arrhythmia, and inotropic effects (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Cytolysin\|Cytolysin]]	+	*[[Cytolysin 3D structures\|Cytolysin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Stihl]]		[[Category: Stihl]]
	[[Category: Bruix, M]]		[[Category: Bruix, M]]

OCA at 07:54, 25 July 2018

OCA — Wed, 25 Jul 2018 07:54:30 GMT

←Older revision		Revision as of 07:54, 25 July 2018
Line 1:		Line 1:
		+
	==NMR structure of the sea anemone actinoporin Sticholysin==		==NMR structure of the sea anemone actinoporin Sticholysin==
	<StructureSection load='2ks4' size='340' side='right' caption='[[2ks4]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2ks4' size='340' side='right' caption='[[2ks4]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ks4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Carribean_sea_anemone Carribean sea anemone~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KS4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ks4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Stihl Stihl]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KS4 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ks3\|2ks3]], [[1kd6\|1kd6]], [[1iaz\|1iaz]], [[1gwy\|1gwy]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ks3\|2ks3]], [[1kd6\|1kd6]], [[1iaz\|1iaz]], [[1gwy\|1gwy]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STCH1, STCH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6123 ~~Carribean sea anemone~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STCH1, STCH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6123 STIHL])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [http://pdbe.org/2ks4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [http://www.ebi.ac.uk/pdbsum/2ks4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [http://pdbe.org/2ks4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [http://www.ebi.ac.uk/pdbsum/2ks4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ks4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/2ks4_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/2ks4_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ks4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 2ks4" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2ks4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytolysin\|Cytolysin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Carribean sea anemone~~]]	+	[[Category: Stihl]]
	[[Category: Bruix, M]]		[[Category: Bruix, M]]
	[[Category: Castrillo, I]]		[[Category: Castrillo, I]]

OCA at 01:29, 10 September 2015

OCA — Thu, 10 Sep 2015 01:29:53 GMT

←Older revision		Revision as of 01:29, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2ks4' size='340' side='right' caption='[[2ks4]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2ks4' size='340' side='right' caption='[[2ks4]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ks4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Stichodactyla_helianthus Stichodactyla helianthus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KS4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ks4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Carribean_sea_anemone Carribean sea anemone]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KS4 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ks3\|2ks3]], [[1kd6\|1kd6]], [[1iaz\|1iaz]], [[1gwy\|1gwy]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ks3\|2ks3]], [[1kd6\|1kd6]], [[1iaz\|1iaz]], [[1gwy\|1gwy]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STCH1, STCH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6123 ~~Stichodactyla helianthus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STCH1, STCH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6123 Carribean sea anemone])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [http://www.ebi.ac.uk/pdbsum/2ks4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [http://pdbe.org/2ks4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [http://www.ebi.ac.uk/pdbsum/2ks4 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ks4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Stichodactyla helianthus~~]]	+	[[Category: Carribean sea anemone]]
	[[Category: Bruix, M]]		[[Category: Bruix, M]]
	[[Category: Castrillo, I]]		[[Category: Castrillo, I]]

OCA at 15:05, 24 December 2014

OCA — Wed, 24 Dec 2014 15:05:38 GMT

←Older revision		Revision as of 15:05, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [http://www.ebi.ac.uk/pdbsum/2ks4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [http://www.ebi.ac.uk/pdbsum/2ks4 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACTP1_STOHE ACTP1_STOHE]] Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and hemolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of several monomers. Cytolytic effects include red blood cells hemolysis, platelet aggregation and lysis, cytotoxic and cytostatic effects on fibroblasts. Lethality in mammals has been ascribed to severe vasospasm of coronary vessels, cardiac arrhythmia, and inotropic effects (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Stichodactyla helianthus]]		[[Category: Stichodactyla helianthus]]
-	[[Category: Bruix, M.]]	+	[[Category: Bruix, M]]
-	[[Category: Castrillo, I.]]	+	[[Category: Castrillo, I]]
-	[[Category: Santoro, J.]]	+	[[Category: Santoro, J]]
	[[Category: Cytolysis]]		[[Category: Cytolysis]]
	[[Category: Hemolysis]]		[[Category: Hemolysis]]

OCA at 15:31, 12 October 2014

OCA — Sun, 12 Oct 2014 15:31:08 GMT

←Older revision		Revision as of 15:31, 12 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2ks4.~~png~~\|~~left~~\|200px]]	+	==NMR structure of the sea anemone actinoporin Sticholysin==
		+	<StructureSection load='2ks4' size='340' side='right' caption='[[2ks4]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ks4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KS4 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ks3\|2ks3]], [[1kd6\|1kd6]], [[1iaz\|1iaz]], [[1gwy\|1gwy]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STCH1, STCH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6123 Stichodactyla helianthus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ks4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ks4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ks4 RCSB], [http://www.ebi.ac.uk/pdbsum/2ks4 PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/2ks4_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sticholysin I is an actinoporin, a pore forming toxin, of 176 aminoacids produced by the sea anemone Stichodactyla heliantus. Isotopically labelled (13)C/(15)N recombinant protein was produced in E. coli. Here we report the complete NMR (15)N, (13)C and (1)H chemical shifts assignments of Stn I at pH 4.0 and 25 degrees C (BMRB No. 15927).

-	~~{{STRUCTURE_2ks4\| PDB=2ks4 \| SCENE= }}~~	+	1H, 13C, and 15N NMR assignments of the actinoporin Sticholysin I.,Castrillo I, Alegre-Cebollada J, del Pozo AM, Gavilanes JG, Santoro J, Bruix M Biomol NMR Assign. 2009 Jun;3(1):5-7. Epub 2008 Nov 6. PMID:19636934<ref>PMID:19636934</ref>

-	~~===NMR structure~~ of the ~~sea anemone actinoporin Sticholysin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19636934}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2ks4]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019636934<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Stichodactyla helianthus]]		[[Category: Stichodactyla helianthus]]
	[[Category: Bruix, M.]]		[[Category: Bruix, M.]]

OCA: Protected "2ks4" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 13:31:54 GMT

Protected "2ks4" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:31, 7 January 2013

OCA at 13:31, 7 January 2013

OCA — Mon, 07 Jan 2013 13:31:50 GMT

←Older revision		Revision as of 13:31, 7 January 2013
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:2ks4.png\|left\|200px]]
-	[[Image:2ks4.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2ks4", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2ks4\| PDB=2ks4 \| SCENE= }}		{{STRUCTURE_2ks4\| PDB=2ks4 \| SCENE= }}

	===NMR structure of the sea anemone actinoporin Sticholysin===		===NMR structure of the sea anemone actinoporin Sticholysin===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19636934}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19636934 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19636934}}		{{ABSTRACT_PUBMED_19636934}}

	==About this Structure==		==About this Structure==
-	~~2KS4~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA].	+	[[2ks4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19636934~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019636934</ref><references group="xtra"/>
	[[Category: Stichodactyla helianthus]]		[[Category: Stichodactyla helianthus]]
	[[Category: Bruix, M.]]		[[Category: Bruix, M.]]
Line 40:		Line 28:
	[[Category: Transport]]		[[Category: Transport]]
	[[Category: Transport protein]]		[[Category: Transport protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 1 09:43:48 2010''

OCA at 05:35, 1 September 2010

OCA — Wed, 01 Sep 2010 05:35:41 GMT

←Older revision		Revision as of 05:35, 1 September 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:2ks4.jpg\|left\|200px]]

-	The ~~entry 2ks4~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2ks4", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2ks4\| PDB=2ks4 \| SCENE= }}

-	~~Authors: Castrillo, I., Santoro, J., Bruix, M.~~	+	===NMR structure of the sea anemone actinoporin Sticholysin===

-	Description: NMR structure of the sea anemone actinoporin Sticholysin

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Aug 11 23~~:27:32 2010''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19636934}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19636934 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19636934}}
		+
		+	==About this Structure==
		+	2KS4 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KS4 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19636934</ref><references group="xtra"/>
		+	[[Category: Stichodactyla helianthus]]
		+	[[Category: Bruix, M.]]
		+	[[Category: Castrillo, I.]]
		+	[[Category: Santoro, J.]]
		+	[[Category: Cytolysis]]
		+	[[Category: Hemolysis]]
		+	[[Category: Ion transport]]
		+	[[Category: Membrane]]
		+	[[Category: Nematocyst]]
		+	[[Category: Porin]]
		+	[[Category: Protein]]
		+	[[Category: Secreted]]
		+	[[Category: Toxin]]
		+	[[Category: Transmembrane]]
		+	[[Category: Transport]]
		+	[[Category: Transport protein]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 1 09:43:48 2010''

OCA at 19:20, 11 August 2010

OCA — Wed, 11 Aug 2010 19:20:14 GMT

←Older revision		Revision as of 19:20, 11 August 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 2ks4 is ON HOLD	+	The entry 2ks4 is ON HOLD until Paper Publication

	Authors: Castrillo, I., Santoro, J., Bruix, M.		Authors: Castrillo, I., Santoro, J., Bruix, M.
Line 7:		Line 7:
	Description: NMR structure of the sea anemone actinoporin Sticholysin		Description: NMR structure of the sea anemone actinoporin Sticholysin

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jan 6 09~~:07:54 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 11 23:27:32 2010''