2kse - Revision history

OCA at 06:48, 1 May 2024

OCA — Wed, 01 May 2024 06:48:23 GMT

←Older revision		Revision as of 06:48, 1 May 2024
Line 1:		Line 1:

	==Backbone structure of the membrane domain of E. coli histidine kinase receptor QseC, Center for Structures of Membrane Proteins (CSMP) target 4311C==		==Backbone structure of the membrane domain of E. coli histidine kinase receptor QseC, Center for Structures of Membrane Proteins (CSMP) target 4311C==
-	<StructureSection load='2kse' size='340' side='right'caption='[[2kse~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2kse' size='340' side='right'caption='[[2kse]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kse]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kse]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KSE FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>b3026, JW2994, qseC, ygiY ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=83333 ECOLI])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kse OCA], [https://pdbe.org/2kse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kse RCSB], [https://www.ebi.ac.uk/pdbsum/2kse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kse ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kse OCA], [https://pdbe.org/2kse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kse RCSB], [https://www.ebi.ac.uk/pdbsum/2kse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kse ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/QSEC_ECOLI QSEC_ECOLI]] Member of a two-component regulatory system QseB/QseC. Activates the flagella regulon by activating transcription of FlhDC. May activate QseB by phosphorylation.<ref>PMID:11929534</ref>	+	[https://www.uniprot.org/uniprot/QSEC_ECOLI QSEC_ECOLI] Member of a two-component regulatory system QseB/QseC. Activates the flagella regulon by activating transcription of FlhDC. May activate QseB by phosphorylation.<ref>PMID:11929534</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kse ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kse ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	NMR structural studies of membrane proteins (MP) are hampered by complications in MP expression, technical difficulties associated with the slow process of NMR spectral peak assignment, and limited distance information obtainable for transmembrane (TM) helices. To overcome the inherent challenges in the determination of MP structures, we have developed a rapid and cost-efficient strategy that combines cell-free (CF) protein synthesis, optimized combinatorial dual-isotope labeling for nearly instant resonance assignment, and fast acquisition of long-distance information using paramagnetic probes. Here we report three backbone structures for the TM domains of the three classes of Escherichia coli histidine kinase receptors (HKRs). The ArcB and QseC TM domains are both two-helical motifs, whereas the KdpD TM domain comprises a four-helical bundle with shorter second and third helices. The interhelical distances (up to 12 A) reveal weak interactions within the TM domains of all three receptors. Determined consecutively within 8 months, these structures offer insight into the abundant and underrepresented in the Protein Data Bank class of 2-4 TM crossers and demonstrate the efficiency of our CF combinatorial dual-labeling strategy, which can be applied to solve MP structures in high numbers and at a high speed. Our results greatly expand the current knowledge of HKR structure, opening the doors to studies on their widespread and pharmaceutically important bacterial signaling mechanism.
-
-	Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis.,Maslennikov I, Klammt C, Hwang E, Kefala G, Okamura M, Esquivies L, Mors K, Glaubitz C, Kwiatkowski W, Jeon YH, Choe S Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10902-7. Epub 2010 May 24. PMID:20498088<ref>PMID:20498088</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2kse" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Histidine kinase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: CSMP, Center for Structures of Membrane Proteins]]~~	+	[[Category: Choe S]]
-	[[Category: Choe, S]]	+	[[Category: Esquivies L]]
-	[[Category: Esquivies, L]]	+	[[Category: Kefala G]]
-	[[Category: Kefala, G]]	+	[[Category: Klammt C]]
-	[[Category: Klammt, C]]	+	[[Category: Kwiatkowski W]]
-	[[Category: Kwiatkowski, W]]	+	[[Category: Maslennikov I]]
-	[[Category: Maslennikov, I]]	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Cell inner membrane]]~~	+
-	~~[[Category: Cell membrane]]~~	+
-	~~[[Category: Cell-free synthesis]]~~	+
-	~~[[Category: Center for structures of membrane protein]]~~	+
-	~~[[Category: Csmp]]~~	+
-	~~[[Category: Histidine kinase receptor]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Membrane domain]]~~	+
-	~~[[Category: Methods development]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: PSI, Protein structure initiative]]~~	+
-	~~[[Category: Structural genomic]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Transmembrane]]~~	+
-	~~[[Category: Two-component regulatory system]]~~	+
-	~~[[Category: Two-helical hairpin~~]]	+

OCA at 07:31, 14 April 2021

OCA — Wed, 14 Apr 2021 07:31:16 GMT

←Older revision		Revision as of 07:31, 14 April 2021
Line 1:		Line 1:

	==Backbone structure of the membrane domain of E. coli histidine kinase receptor QseC, Center for Structures of Membrane Proteins (CSMP) target 4311C==		==Backbone structure of the membrane domain of E. coli histidine kinase receptor QseC, Center for Structures of Membrane Proteins (CSMP) target 4311C==
-	<StructureSection load='2kse' size='340' side='right' caption='[[2kse]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2kse' size='340' side='right'caption='[[2kse]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kse]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2KSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kse]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KSE FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3026, JW2994, qseC, ygiY ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3026, JW2994, qseC, ygiY ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2kse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kse OCA], [~~http~~://pdbe.org/2kse PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2kse RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2kse PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2kse ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kse OCA], [https://pdbe.org/2kse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kse RCSB], [https://www.ebi.ac.uk/pdbsum/2kse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kse ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/QSEC_ECOLI QSEC_ECOLI]] Member of a two-component regulatory system QseB/QseC. Activates the flagella regulon by activating transcription of FlhDC. May activate QseB by phosphorylation.<ref>PMID:11929534</ref>	+	[[https://www.uniprot.org/uniprot/QSEC_ECOLI QSEC_ECOLI]] Member of a two-component regulatory system QseB/QseC. Activates the flagella regulon by activating transcription of FlhDC. May activate QseB by phosphorylation.<ref>PMID:11929534</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Ecoli]]		[[Category: Ecoli]]
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
		+	[[Category: Large Structures]]
	[[Category: CSMP, Center for Structures of Membrane Proteins]]		[[Category: CSMP, Center for Structures of Membrane Proteins]]
	[[Category: Choe, S]]		[[Category: Choe, S]]

OCA at 08:14, 25 July 2018

OCA — Wed, 25 Jul 2018 08:14:45 GMT

←Older revision		Revision as of 08:14, 25 July 2018
Line 1:		Line 1:
		+
	==Backbone structure of the membrane domain of E. coli histidine kinase receptor QseC, Center for Structures of Membrane Proteins (CSMP) target 4311C==		==Backbone structure of the membrane domain of E. coli histidine kinase receptor QseC, Center for Structures of Membrane Proteins (CSMP) target 4311C==
	<StructureSection load='2kse' size='340' side='right' caption='[[2kse]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kse' size='340' side='right' caption='[[2kse]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3026, JW2994, qseC, ygiY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3026, JW2994, qseC, ygiY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kse OCA], [http://pdbe.org/2kse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kse RCSB], [http://www.ebi.ac.uk/pdbsum/2kse PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kse OCA], [http://pdbe.org/2kse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kse RCSB], [http://www.ebi.ac.uk/pdbsum/2kse PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kse ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/2kse_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/2kse_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 17:04, 7 February 2016

OCA — Sun, 07 Feb 2016 17:04:08 GMT

←Older revision		Revision as of 17:04, 7 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kse ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:28, 10 September 2015

OCA — Thu, 10 Sep 2015 03:28:50 GMT

←Older revision		Revision as of 03:28, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2kse' size='340' side='right' caption='[[2kse]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kse' size='340' side='right' caption='[[2kse]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KSE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KSE FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3026, JW2994, qseC, ygiY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ~~Escherichia coli K-12~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3026, JW2994, qseC, ygiY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kse RCSB], [http://www.ebi.ac.uk/pdbsum/2kse PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kse OCA], [http://pdbe.org/2kse PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kse RCSB], [http://www.ebi.ac.uk/pdbsum/2kse PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2kse" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli k-12~~]]	+	[[Category: Ecoli]]
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
	[[Category: CSMP, Center for Structures of Membrane Proteins]]		[[Category: CSMP, Center for Structures of Membrane Proteins]]

OCA at 16:53, 24 December 2014

OCA — Wed, 24 Dec 2014 16:53:48 GMT

←Older revision		Revision as of 16:53, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KSE FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KSE FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3026, JW2994, qseC, ygiY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3026, JW2994, qseC, ygiY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kse RCSB], [http://www.ebi.ac.uk/pdbsum/2kse PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kse RCSB], [http://www.ebi.ac.uk/pdbsum/2kse PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/QSEC_ECOLI QSEC_ECOLI]] Member of a two-component regulatory system QseB/QseC. Activates the flagella regulon by activating transcription of FlhDC. May activate QseB by phosphorylation.<ref>PMID:11929534</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
-	[[Category: CSMP, Center for Structures of Membrane Proteins.]]	+	[[Category: CSMP, Center for Structures of Membrane Proteins]]
-	[[Category: Choe, S.]]	+	[[Category: Choe, S]]
-	[[Category: Esquivies, L.]]	+	[[Category: Esquivies, L]]
-	[[Category: Kefala, G.]]	+	[[Category: Kefala, G]]
-	[[Category: Klammt, C.]]	+	[[Category: Klammt, C]]
-	[[Category: Kwiatkowski, W.]]	+	[[Category: Kwiatkowski, W]]
-	[[Category: Maslennikov, I.]]	+	[[Category: Maslennikov, I]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Cell inner membrane]]		[[Category: Cell inner membrane]]
Line 51:		Line 53:
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
-	[[Category: Protein structure initiative]]	+	[[Category: PSI, Protein structure initiative]]
-	~~[[Category: Psi-2~~]]	+
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 10:04, 29 September 2014

OCA — Mon, 29 Sep 2014 10:04:12 GMT

←Older revision		Revision as of 10:04, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2kse.~~png\|left~~\|200px]]	+	==Backbone structure of the membrane domain of E. coli histidine kinase receptor QseC, Center for Structures of Membrane Proteins (CSMP) target 4311C==
		+	<StructureSection load='2kse' size='340' side='right' caption='[[2kse]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2kse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KSE FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b3026, JW2994, qseC, ygiY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kse RCSB], [http://www.ebi.ac.uk/pdbsum/2kse PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/2kse_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	NMR structural studies of membrane proteins (MP) are hampered by complications in MP expression, technical difficulties associated with the slow process of NMR spectral peak assignment, and limited distance information obtainable for transmembrane (TM) helices. To overcome the inherent challenges in the determination of MP structures, we have developed a rapid and cost-efficient strategy that combines cell-free (CF) protein synthesis, optimized combinatorial dual-isotope labeling for nearly instant resonance assignment, and fast acquisition of long-distance information using paramagnetic probes. Here we report three backbone structures for the TM domains of the three classes of Escherichia coli histidine kinase receptors (HKRs). The ArcB and QseC TM domains are both two-helical motifs, whereas the KdpD TM domain comprises a four-helical bundle with shorter second and third helices. The interhelical distances (up to 12 A) reveal weak interactions within the TM domains of all three receptors. Determined consecutively within 8 months, these structures offer insight into the abundant and underrepresented in the Protein Data Bank class of 2-4 TM crossers and demonstrate the efficiency of our CF combinatorial dual-labeling strategy, which can be applied to solve MP structures in high numbers and at a high speed. Our results greatly expand the current knowledge of HKR structure, opening the doors to studies on their widespread and pharmaceutically important bacterial signaling mechanism.

-	~~{{STRUCTURE_2kse\| PDB=2kse \| SCENE= }}~~	+	Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis.,Maslennikov I, Klammt C, Hwang E, Kefala G, Okamura M, Esquivies L, Mors K, Glaubitz C, Kwiatkowski W, Jeon YH, Choe S Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10902-7. Epub 2010 May 24. PMID:20498088<ref>PMID:20498088</ref>

-	~~===Backbone structure~~ of the ~~membrane domain~~ of E. ~~coli histidine kinase receptor QseC, Center for Structures of Membrane Proteins (CSMP) target 4311C===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20498088}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[2kse]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA].~~	+	</StructureSection>
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]

OCA: Protected "2kse" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 09:44:04 GMT

Protected "2kse" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:44, 5 December 2012

OCA at 09:44, 5 December 2012

OCA — Wed, 05 Dec 2012 09:44:02 GMT

←Older revision		Revision as of 09:44, 5 December 2012
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:2kse.png\|left\|200px]]
-	[[Image:2kse.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2kse", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2kse\| PDB=2kse \| SCENE= }}		{{STRUCTURE_2kse\| PDB=2kse \| SCENE= }}

	===Backbone structure of the membrane domain of E. coli histidine kinase receptor QseC, Center for Structures of Membrane Proteins (CSMP) target 4311C===		===Backbone structure of the membrane domain of E. coli histidine kinase receptor QseC, Center for Structures of Membrane Proteins (CSMP) target 4311C===

		+	{{ABSTRACT_PUBMED_20498088}}

	==About this Structure==		==About this Structure==
-	~~2KSE~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli~~ Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA].	+	[[2kse]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA].
-	[[Category: Escherichia coli]]	+	[[Category: Escherichia coli k-12]]
	[[Category: Histidine kinase]]		[[Category: Histidine kinase]]
	[[Category: CSMP, Center for Structures of Membrane Proteins.]]		[[Category: CSMP, Center for Structures of Membrane Proteins.]]
Line 44:		Line 38:
	[[Category: Two-component regulatory system]]		[[Category: Two-component regulatory system]]
	[[Category: Two-helical hairpin]]		[[Category: Two-helical hairpin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 3 16:50:05 2010''

OCA at 14:50, 3 March 2010

OCA — Wed, 03 Mar 2010 14:50:06 GMT

←Older revision		Revision as of 14:50, 3 March 2010
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:2kse.jpg\|left\|200px]]

-	The ~~entry 2kse~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2kse", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2kse\| PDB=2kse \| SCENE= }}

-	~~Authors: Maslennikov, I., Klammt, C., Kefala, G., Esquivies, L., Kwiatkowski, W., Choe, S~~., Center for Structures of Membrane Proteins (CSMP)	+	===Backbone structure of the membrane domain of E. coli histidine kinase receptor QseC, Center for Structures of Membrane Proteins (CSMP) target 4311C===

-	Description: Backbone structure of the membrane domain of E. coli histidine kinase receptor QseC

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jan 13 13~~:27:34 2010''	+	==About this Structure==
		+	2KSE is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSE OCA].
		+	[[Category: Escherichia coli]]
		+	[[Category: Histidine kinase]]
		+	[[Category: CSMP, Center for Structures of Membrane Proteins.]]
		+	[[Category: Choe, S.]]
		+	[[Category: Esquivies, L.]]
		+	[[Category: Kefala, G.]]
		+	[[Category: Klammt, C.]]
		+	[[Category: Kwiatkowski, W.]]
		+	[[Category: Maslennikov, I.]]
		+	[[Category: Atp-binding]]
		+	[[Category: Cell inner membrane]]
		+	[[Category: Cell membrane]]
		+	[[Category: Cell-free synthesis]]
		+	[[Category: Center for structures of membrane protein]]
		+	[[Category: Csmp]]
		+	[[Category: Histidine kinase receptor]]
		+	[[Category: Kinase]]
		+	[[Category: Membrane]]
		+	[[Category: Membrane domain]]
		+	[[Category: Methods development]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Phosphoprotein]]
		+	[[Category: Protein structure initiative]]
		+	[[Category: Psi-2]]
		+	[[Category: Structural genomic]]
		+	[[Category: Transferase]]
		+	[[Category: Transmembrane]]
		+	[[Category: Two-component regulatory system]]
		+	[[Category: Two-helical hairpin]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 3 16:50:05 2010''