2ksz - Revision history

OCA at 06:48, 1 May 2024

OCA — Wed, 01 May 2024 06:48:31 GMT

←Older revision		Revision as of 06:48, 1 May 2024
Line 1:		Line 1:

	==The solution structure of the Magnesium bound soybean calmodulin isoform 4 N-domain==		==The solution structure of the Magnesium bound soybean calmodulin isoform 4 N-domain==
-	<StructureSection load='2ksz' size='340' side='right'caption='[[2ksz~~]], [[NMR_Ensembles_of_Models \| 5 NMR models~~]]' scene=''>	+	<StructureSection load='2ksz' size='340' side='right'caption='[[2ksz]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ksz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Glycine_hispida~~ Glycine ~~hispida~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KSZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ksz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KSZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[2roa\|2roa]], [[1f70\|1f70]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">SCaM-4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3847 Glycine hispida])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ksz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksz OCA], [https://pdbe.org/2ksz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ksz RCSB], [https://www.ebi.ac.uk/pdbsum/2ksz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ksz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ksz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksz OCA], [https://pdbe.org/2ksz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ksz RCSB], [https://www.ebi.ac.uk/pdbsum/2ksz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ksz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q39890_SOYBN Q39890_SOYBN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ksz ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ksz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Soybean calmodulin isoform 4 (sCaM4) is a plant calcium binding protein, regulating cellular responses to the second messenger Ca(2+). We have found that the metal ion free (apo-) form of sCaM4 possesses a half unfolded structure, with the N-terminal domain unfolded and the C-terminal domain folded. This result was unexpected as the apo-forms of both soybean calmodulin isoform 1 (sCaM1) and mammalian CaM (mCaM) are fully folded. Due to the fact that free Mg(2+) ions are always present at high concentrations in cells (0.5 approximately 2 mM), we suggest that Mg(2+) should be bound to sCaM4 in non-activated cells. CD studies revealed that in the presence of Mg(2+) the initially unfolded N-terminal domain of sCaM4 folds into an alpha-helix-rich structure, similar to the Ca(2+)-form. We have used the NMR backbone residual dipolar coupling (RDC) restraints (1)D(NH), (1)D(CalphaHalpha), and (1)D(C)'(Calpha) to determine the solution structure of the N-terminal domain of Mg(2+)-sCaM4 (Mg(2+)-sCaM4-NT). Compared with the known structure of Ca(2+)-sCaM4, the structure of the Mg(2+)-sCaM4-NT does not fully open the hydrophobic pocket, which was further confirmed by the use of the fluorescent probe ANS. Tryptophan fluorescence experiments were used to study the interactions between Mg(2+)-sCaM4 and CaM-binding peptides derived from smooth muscle myosin light chain kinase as well as plant glutamate decarboxylase. These results suggest that Mg(2+)-sCaM4 does not bind to Ca(2+)-CaM target peptides, and therefore is functionally similar to apo-mCaM. The Mg(2+)- and apo- structures of the sCaM4-NT provide unique insights into the structure and function of some plant calmodulins in resting cells.
-
-	The solution structure of the Mg(2+)- form of soybean calmodulin isoform 4 reveals unique features of plant calmodulins in resting cells.,Huang H, Ishida H, Vogel HJ Protein Sci. 2010 Jan 6. PMID:20054830<ref>PMID:20054830</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2ksz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Calmodulin 3D structures\|Calmodulin 3D structures]]		*[[Calmodulin 3D structures\|Calmodulin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Glycine ~~hispida~~]]	+	[[Category: Glycine max]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Huang, H]]	+	[[Category: Huang H]]
-	[[Category: Ishida, H]]	+	[[Category: Ishida H]]
-	[[Category: Vogel~~, H J]]~~	+	[[Category: Vogel HJ]]
-	~~[[Category: Magnesium]]~~	+
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Residual dipolar coupling]]~~	+
-	~~[[Category: Soybean calmodulin isoform 4~~]]	+

OCA at 07:31, 14 April 2021

OCA — Wed, 14 Apr 2021 07:31:40 GMT

←Older revision		Revision as of 07:31, 14 April 2021
Line 1:		Line 1:

	==The solution structure of the Magnesium bound soybean calmodulin isoform 4 N-domain==		==The solution structure of the Magnesium bound soybean calmodulin isoform 4 N-domain==
-	<StructureSection load='2ksz' size='340' side='right' caption='[[2ksz]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>	+	<StructureSection load='2ksz' size='340' side='right'caption='[[2ksz]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ksz]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Glycine_hispida Glycine hispida]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2KSZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ksz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycine_hispida Glycine hispida]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KSZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roa\|2roa]], [[1f70\|1f70]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2roa\|2roa]], [[1f70\|1f70]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCaM-4 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3847 Glycine hispida])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCaM-4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3847 Glycine hispida])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ksz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksz OCA], [~~http~~://pdbe.org/2ksz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ksz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ksz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ksz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ksz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksz OCA], [https://pdbe.org/2ksz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ksz RCSB], [https://www.ebi.ac.uk/pdbsum/2ksz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ksz ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Calmodulin\|Calmodulin]]	+	*[[Calmodulin 3D structures\|Calmodulin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Glycine hispida]]		[[Category: Glycine hispida]]
		+	[[Category: Large Structures]]
	[[Category: Huang, H]]		[[Category: Huang, H]]
	[[Category: Ishida, H]]		[[Category: Ishida, H]]

OCA at 08:37, 25 July 2018

OCA — Wed, 25 Jul 2018 08:37:42 GMT

←Older revision		Revision as of 08:37, 25 July 2018
Line 1:		Line 1:
		+
	==The solution structure of the Magnesium bound soybean calmodulin isoform 4 N-domain==		==The solution structure of the Magnesium bound soybean calmodulin isoform 4 N-domain==
	<StructureSection load='2ksz' size='340' side='right' caption='[[2ksz]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>		<StructureSection load='2ksz' size='340' side='right' caption='[[2ksz]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roa\|2roa]], [[1f70\|1f70]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roa\|2roa]], [[1f70\|1f70]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCaM-4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3847 Glycine hispida])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCaM-4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3847 Glycine hispida])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ksz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksz OCA], [http://pdbe.org/2ksz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ksz RCSB], [http://www.ebi.ac.uk/pdbsum/2ksz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ksz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksz OCA], [http://pdbe.org/2ksz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ksz RCSB], [http://www.ebi.ac.uk/pdbsum/2ksz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ksz ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/2ksz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/2ksz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ksz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:36, 11 September 2015

OCA — Fri, 11 Sep 2015 09:36:16 GMT

←Older revision		Revision as of 09:36, 11 September 2015
Line 6:		Line 6:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roa\|2roa]], [[1f70\|1f70]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roa\|2roa]], [[1f70\|1f70]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCaM-4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3847 Glycine hispida])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCaM-4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3847 Glycine hispida])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ksz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ksz RCSB], [http://www.ebi.ac.uk/pdbsum/2ksz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ksz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksz OCA], [http://pdbe.org/2ksz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ksz RCSB], [http://www.ebi.ac.uk/pdbsum/2ksz PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ksz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 12:28, 10 December 2014

OCA — Wed, 10 Dec 2014 12:28:05 GMT

←Older revision		Revision as of 12:28, 10 December 2014
Line 2:		Line 2:
	<StructureSection load='2ksz' size='340' side='right' caption='[[2ksz]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>		<StructureSection load='2ksz' size='340' side='right' caption='[[2ksz]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ksz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Glycine_max~~ Glycine ~~max~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KSZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ksz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Glycine_hispida Glycine hispida]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KSZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roa\|2roa]], [[1f70\|1f70]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roa\|2roa]], [[1f70\|1f70]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCaM-4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3847 Glycine ~~max~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCaM-4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3847 Glycine hispida])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ksz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ksz RCSB], [http://www.ebi.ac.uk/pdbsum/2ksz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ksz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ksz RCSB], [http://www.ebi.ac.uk/pdbsum/2ksz PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Glycine ~~max~~]]	+	[[Category: Glycine hispida]]
-	[[Category: Huang, H.]]	+	[[Category: Huang, H]]
-	[[Category: Ishida, H.]]	+	[[Category: Ishida, H]]
-	[[Category: Vogel, H J.]]	+	[[Category: Vogel, H J]]
	[[Category: Magnesium]]		[[Category: Magnesium]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]
	[[Category: Residual dipolar coupling]]		[[Category: Residual dipolar coupling]]
	[[Category: Soybean calmodulin isoform 4]]		[[Category: Soybean calmodulin isoform 4]]

OCA at 09:22, 29 September 2014

OCA — Mon, 29 Sep 2014 09:22:34 GMT

←Older revision		Revision as of 09:22, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2ksz.~~png~~\|~~left~~\|200px]]	+	==The solution structure of the Magnesium bound soybean calmodulin isoform 4 N-domain==
		+	<StructureSection load='2ksz' size='340' side='right' caption='[[2ksz]], [[NMR_Ensembles_of_Models \| 5 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ksz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KSZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2roa\|2roa]], [[1f70\|1f70]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCaM-4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3847 Glycine max])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ksz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ksz RCSB], [http://www.ebi.ac.uk/pdbsum/2ksz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ks/2ksz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Soybean calmodulin isoform 4 (sCaM4) is a plant calcium binding protein, regulating cellular responses to the second messenger Ca(2+). We have found that the metal ion free (apo-) form of sCaM4 possesses a half unfolded structure, with the N-terminal domain unfolded and the C-terminal domain folded. This result was unexpected as the apo-forms of both soybean calmodulin isoform 1 (sCaM1) and mammalian CaM (mCaM) are fully folded. Due to the fact that free Mg(2+) ions are always present at high concentrations in cells (0.5 approximately 2 mM), we suggest that Mg(2+) should be bound to sCaM4 in non-activated cells. CD studies revealed that in the presence of Mg(2+) the initially unfolded N-terminal domain of sCaM4 folds into an alpha-helix-rich structure, similar to the Ca(2+)-form. We have used the NMR backbone residual dipolar coupling (RDC) restraints (1)D(NH), (1)D(CalphaHalpha), and (1)D(C)'(Calpha) to determine the solution structure of the N-terminal domain of Mg(2+)-sCaM4 (Mg(2+)-sCaM4-NT). Compared with the known structure of Ca(2+)-sCaM4, the structure of the Mg(2+)-sCaM4-NT does not fully open the hydrophobic pocket, which was further confirmed by the use of the fluorescent probe ANS. Tryptophan fluorescence experiments were used to study the interactions between Mg(2+)-sCaM4 and CaM-binding peptides derived from smooth muscle myosin light chain kinase as well as plant glutamate decarboxylase. These results suggest that Mg(2+)-sCaM4 does not bind to Ca(2+)-CaM target peptides, and therefore is functionally similar to apo-mCaM. The Mg(2+)- and apo- structures of the sCaM4-NT provide unique insights into the structure and function of some plant calmodulins in resting cells.

-	~~{{STRUCTURE_2ksz\| PDB=2ksz \| SCENE= }}~~	+	The solution structure of the Mg(2+)- form of soybean calmodulin isoform 4 reveals unique features of plant calmodulins in resting cells.,Huang H, Ishida H, Vogel HJ Protein Sci. 2010 Jan 6. PMID:20054830<ref>PMID:20054830</ref>

-	~~===The solution structure~~ of the ~~Magnesium bound soybean calmodulin isoform 4 N-domain===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20054830}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2ksz]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Glycine_max Glycine max]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA].~~	+

	==See Also==		==See Also==
	*[[Calmodulin\|Calmodulin]]		*[[Calmodulin\|Calmodulin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020054830<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Glycine max]]		[[Category: Glycine max]]
	[[Category: Huang, H.]]		[[Category: Huang, H.]]

OCA at 17:53, 20 October 2012

OCA — Sat, 20 Oct 2012 17:53:04 GMT

←Older revision		Revision as of 17:53, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2ksz]] is a 1 chain structure ~~of [[Calmodulin]]~~ with sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA].	+	[[2ksz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA].

	==See Also==		==See Also==

OCA: Protected "2ksz" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 02:18:08 GMT

Protected "2ksz" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:18, 27 July 2012

OCA at 02:18, 27 July 2012

OCA — Fri, 27 Jul 2012 02:18:06 GMT

←Older revision		Revision as of 02:18, 27 July 2012
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-	{{Seed}}
	[[Image:2ksz.png\|left\|200px]]		[[Image:2ksz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2ksz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2ksz\| PDB=2ksz \| SCENE= }}		{{STRUCTURE_2ksz\| PDB=2ksz \| SCENE= }}

	===The solution structure of the Magnesium bound soybean calmodulin isoform 4 N-domain===		===The solution structure of the Magnesium bound soybean calmodulin isoform 4 N-domain===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20054830}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20054830 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20054830}}		{{ABSTRACT_PUBMED_20054830}}

	==About this Structure==		==About this Structure==
-	~~2KSZ~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA].	+	[[2ksz]] is a 1 chain structure of [[Calmodulin]] with sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA].
		+
		+	==See Also==
		+	*[[Calmodulin\|Calmodulin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20054830~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020054830</ref><references group="xtra"/>
	[[Category: Glycine max]]		[[Category: Glycine max]]
	[[Category: Huang, H.]]		[[Category: Huang, H.]]
Line 32:		Line 23:
	[[Category: Residual dipolar coupling]]		[[Category: Residual dipolar coupling]]
	[[Category: Soybean calmodulin isoform 4]]		[[Category: Soybean calmodulin isoform 4]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 5 11:25:53 2010''

OCA at 08:25, 5 May 2010

OCA — Wed, 05 May 2010 08:25:54 GMT

←Older revision		Revision as of 08:25, 5 May 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:2ksz.~~jpg~~\|left\|200px]]	+	[[Image:2ksz.png\|left\|200px]]

	<!--		<!--
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	===The solution structure of the Magnesium bound soybean calmodulin isoform 4 N-domain===		===The solution structure of the Magnesium bound soybean calmodulin isoform 4 N-domain===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20054830}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20054830 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20054830}}

	==About this Structure==		==About this Structure==
	2KSZ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA].		2KSZ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSZ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20054830</ref><references group="xtra"/>
	[[Category: Glycine max]]		[[Category: Glycine max]]
	[[Category: Huang, H.]]		[[Category: Huang, H.]]
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	[[Category: Soybean calmodulin isoform 4]]		[[Category: Soybean calmodulin isoform 4]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar 10 14~~:17:55 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 5 11:25:53 2010''