2kuq - Revision history

OCA at 06:48, 1 May 2024

OCA — Wed, 01 May 2024 06:48:53 GMT

←Older revision		Revision as of 06:48, 1 May 2024
Line 1:		Line 1:

	==Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK==		==Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK==
-	<StructureSection load='2kuq' size='340' side='right'caption='[[2kuq~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2kuq' size='340' side='right'caption='[[2kuq]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kuq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KUQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kuq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KUQ FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2yt2\|2yt2]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kuq OCA], [https://pdbe.org/2kuq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kuq RCSB], [https://www.ebi.ac.uk/pdbsum/2kuq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kuq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kuq OCA], [https://pdbe.org/2kuq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kuq RCSB], [https://www.ebi.ac.uk/pdbsum/2kuq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kuq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/ALK_HUMAN ALK_HUMAN] Note=A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas. Note=A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A. Note=A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17. Defects in ALK are the cause of susceptibility to neuroblastoma type 3 (NBLST3) [MIM:[https://omim.org/entry/613014 613014]. Neuroblastoma is a common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system.<ref>PMID:18724359</ref> <ref>PMID:18923523</ref> <ref>PMID:18923525</ref> Note=The ALK signaling pathway plays an important role in glioblastoma, the most common malignant brain tumor of adults and one of the most lethal cancers. It regulates both glioblastoma migration and growth.
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FRS3_HUMAN FRS3_HUMAN]] Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and nuclear translocation of ERK2.<ref>PMID:15094036</ref>	+	[https://www.uniprot.org/uniprot/FRS3_HUMAN FRS3_HUMAN] Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and nuclear translocation of ERK2.<ref>PMID:15094036</ref> [https://www.uniprot.org/uniprot/ALK_HUMAN ALK_HUMAN] Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.<ref>PMID:11387242</ref> <ref>PMID:11121404</ref> <ref>PMID:11278720</ref> <ref>PMID:11809760</ref> <ref>PMID:12107166</ref> <ref>PMID:12122009</ref> <ref>PMID:15226403</ref> <ref>PMID:15908427</ref> <ref>PMID:16317043</ref> <ref>PMID:17274988</ref> <ref>PMID:16878150</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kuq ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kuq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The nucleophosmin-anaplastic lymphoma kinase (NPM-ALK) fusion oncoprotein, formed by the t(2;5) chromosomal translocation in anaplastic large-cell lymphomas, has constitutive tyrosine kinase activity and interacts with a number of signaling molecules. One of the interacting partners of NPM-ALK is the adaptor protein, Suc1-associated neurotrophic factor-induced tyrosine-phosphorylated target (SNT), and mutations that deprive NPM-ALK of all three of the SNT-binding sites significantly reduced the transforming activity. In this study, the interactions of the three binding sites in NPM-ALK with the phosphotyrosine binding (PTB) domain of SNT-2 were analyzed. First, by isothermal titration calorimetry, we found that the phosphorylation-independent binding site in NPM-ALK interacts with the SNT-2 PTB domain more tightly than the phosphorylation-dependent binding sites. Second, the solution structure of the SNT-2 PTB domain in complex with the nonphosphorylated NPM-ALK peptide was determined by nuclear magnetic resonance spectroscopy. The NPM-ALK peptide interacts with the hydrophobic surface of the PTB domain and intermolecularly extends the PTB beta-sheet. This interaction mode is much broader and more extensive than those of the phosphorylation-dependent binding sites. Our results indicate that the higher binding activity of the phosphorylation-independent binding site is caused by additional hydrophobic interactions.
-
-	Structural basis for the recognition of nucleophosmin-anaplastic lymphoma kinase oncoprotein by the phosphotyrosine binding domain of Suc1-associated neurotrophic factor-induced tyrosine-phosphorylated target-2.,Koshiba S, Li H, Motoda Y, Tomizawa T, Kasai T, Tochio N, Yabuki T, Harada T, Watanabe S, Tanaka A, Shirouzu M, Kigawa T, Yamamoto T, Yokoyama S J Struct Funct Genomics. 2010 May 8. PMID:20454865<ref>PMID:20454865</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2kuq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Harada, T]]	+	[[Category: Synthetic construct]]
-	[[Category: Kigawa, T]]	+	[[Category: Harada T]]
-	[[Category: Koshiba, S]]	+	[[Category: Kigawa T]]
-	[[Category: Li, H]]	+	[[Category: Koshiba S]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Li H]]
-	[[Category: Tomizawa, T]]	+	[[Category: Tomizawa T]]
-	[[Category: Watanabe, S]]	+	[[Category: Watanabe S]]
-	[[Category: Yokoyama, S]]	+	[[Category: Yokoyama S]]
-	~~[[Category: Chimera]]~~	+
-	~~[[Category: Halk]]~~	+
-	~~[[Category: National project on protein structural and functional analyse]]~~	+
-	~~[[Category: Nppsfa]]~~	+
-	~~[[Category: Ptb domain]]~~	+
-	~~[[Category: Rsgi]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Snt-2~~]]	+

OCA at 07:32, 14 April 2021

OCA — Wed, 14 Apr 2021 07:32:36 GMT

←Older revision		Revision as of 07:32, 14 April 2021
Line 1:		Line 1:

	==Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK==		==Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK==
-	<StructureSection load='2kuq' size='340' side='right' caption='[[2kuq]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2kuq' size='340' side='right'caption='[[2kuq]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kuq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KUQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2KUQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kuq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KUQ FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yt2\|2yt2]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2yt2\|2yt2]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">~~FRS3~~ ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2kuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kuq OCA], [~~http~~://pdbe.org/2kuq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2kuq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2kuq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2kuq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kuq OCA], [https://pdbe.org/2kuq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kuq RCSB], [https://www.ebi.ac.uk/pdbsum/2kuq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kuq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FRS3_HUMAN FRS3_HUMAN]] Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and nuclear translocation of ERK2.<ref>PMID:15094036</ref>	+	[[https://www.uniprot.org/uniprot/FRS3_HUMAN FRS3_HUMAN]] Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and nuclear translocation of ERK2.<ref>PMID:15094036</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Harada, T]]		[[Category: Harada, T]]
	[[Category: Kigawa, T]]		[[Category: Kigawa, T]]

OCA at 08:06, 25 July 2018

OCA — Wed, 25 Jul 2018 08:06:31 GMT

←Older revision		Revision as of 08:06, 25 July 2018
Line 1:		Line 1:
		+
	==Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK==		==Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK==
	<StructureSection load='2kuq' size='340' side='right' caption='[[2kuq]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kuq' size='340' side='right' caption='[[2kuq]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yt2\|2yt2]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yt2\|2yt2]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kuq OCA], [http://pdbe.org/2kuq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kuq RCSB], [http://www.ebi.ac.uk/pdbsum/2kuq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kuq OCA], [http://pdbe.org/2kuq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kuq RCSB], [http://www.ebi.ac.uk/pdbsum/2kuq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kuq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/2kuq_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/2kuq_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kuq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 37:		Line 38:
	[[Category: Koshiba, S]]		[[Category: Koshiba, S]]
	[[Category: Li, H]]		[[Category: Li, H]]
		+	[[Category: Structural genomic]]
	[[Category: Tomizawa, T]]		[[Category: Tomizawa, T]]
	[[Category: Watanabe, S]]		[[Category: Watanabe, S]]
Line 45:		Line 47:
	[[Category: Nppsfa]]		[[Category: Nppsfa]]
	[[Category: Ptb domain]]		[[Category: Ptb domain]]
-	[[Category: Structural genomic]]
	[[Category: Rsgi]]		[[Category: Rsgi]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]
	[[Category: Snt-2]]		[[Category: Snt-2]]

OCA at 17:49, 10 September 2015

OCA — Thu, 10 Sep 2015 17:49:58 GMT

←Older revision		Revision as of 17:49, 10 September 2015
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yt2\|2yt2]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yt2\|2yt2]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kuq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kuq RCSB], [http://www.ebi.ac.uk/pdbsum/2kuq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kuq OCA], [http://pdbe.org/2kuq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kuq RCSB], [http://www.ebi.ac.uk/pdbsum/2kuq PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2kuq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 03:51, 25 December 2014

OCA — Thu, 25 Dec 2014 03:51:49 GMT

←Older revision		Revision as of 03:51, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kuq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kuq RCSB], [http://www.ebi.ac.uk/pdbsum/2kuq PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kuq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kuq RCSB], [http://www.ebi.ac.uk/pdbsum/2kuq PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FRS3_HUMAN FRS3_HUMAN]] Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and nuclear translocation of ERK2.<ref>PMID:15094036</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 16:48, 21 December 2014

OCA — Sun, 21 Dec 2014 16:48:39 GMT

←Older revision		Revision as of 16:48, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2kuq\| PDB=2kuq \| SCENE= }}~~	+	==Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK==
-	===Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK===	+	<StructureSection load='2kuq' size='340' side='right' caption='[[2kuq]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~{{ABSTRACT_PUBMED_20454865}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2kuq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KUQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KUQ FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yt2\|2yt2]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FRS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kuq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kuq RCSB], [http://www.ebi.ac.uk/pdbsum/2kuq PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ku/2kuq_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The nucleophosmin-anaplastic lymphoma kinase (NPM-ALK) fusion oncoprotein, formed by the t(2;5) chromosomal translocation in anaplastic large-cell lymphomas, has constitutive tyrosine kinase activity and interacts with a number of signaling molecules. One of the interacting partners of NPM-ALK is the adaptor protein, Suc1-associated neurotrophic factor-induced tyrosine-phosphorylated target (SNT), and mutations that deprive NPM-ALK of all three of the SNT-binding sites significantly reduced the transforming activity. In this study, the interactions of the three binding sites in NPM-ALK with the phosphotyrosine binding (PTB) domain of SNT-2 were analyzed. First, by isothermal titration calorimetry, we found that the phosphorylation-independent binding site in NPM-ALK interacts with the SNT-2 PTB domain more tightly than the phosphorylation-dependent binding sites. Second, the solution structure of the SNT-2 PTB domain in complex with the nonphosphorylated NPM-ALK peptide was determined by nuclear magnetic resonance spectroscopy. The NPM-ALK peptide interacts with the hydrophobic surface of the PTB domain and intermolecularly extends the PTB beta-sheet. This interaction mode is much broader and more extensive than those of the phosphorylation-dependent binding sites. Our results indicate that the higher binding activity of the phosphorylation-independent binding site is caused by additional hydrophobic interactions.

-	~~==Function==~~	+	Structural basis for the recognition of nucleophosmin-anaplastic lymphoma kinase oncoprotein by the phosphotyrosine binding domain of Suc1-associated neurotrophic factor-induced tyrosine-phosphorylated target-2.,Koshiba S, Li H, Motoda Y, Tomizawa T, Kasai T, Tochio N, Yabuki T, Harada T, Watanabe S, Tanaka A, Shirouzu M, Kigawa T, Yamamoto T, Yokoyama S J Struct Funct Genomics. 2010 May 8. PMID:20454865<ref>PMID:20454865</ref>
-	~~[[http://www.uniprot.org/uniprot/FRS3_HUMAN FRS3_HUMAN]] Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in~~ the ~~activation~~ of ~~MAP kinases. Down~~-~~regulates ERK2 signaling~~ by ~~interfering with~~ the ~~phosphorylation and nuclear translocation~~ of ~~ERK2~~.<ref>PMID:~~15094036~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[2kuq]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Human Human]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2KUQ OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:020454865</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Human]]		[[Category: Human]]
-	[[Category: Harada, T.]]	+	[[Category: Harada, T]]
-	[[Category: Kigawa, T.]]	+	[[Category: Kigawa, T]]
-	[[Category: Koshiba, S.]]	+	[[Category: Koshiba, S]]
-	[[Category: Li, H.]]	+	[[Category: Li, H]]
-	[[Category: Tomizawa, T.]]	+	[[Category: Tomizawa, T]]
-	[[Category: Watanabe, S.]]	+	[[Category: Watanabe, S]]
-	[[Category: Yokoyama, S.]]	+	[[Category: Yokoyama, S]]
	[[Category: Chimera]]		[[Category: Chimera]]
	[[Category: Halk]]		[[Category: Halk]]
Line 24:		Line 42:
	[[Category: Nppsfa]]		[[Category: Nppsfa]]
	[[Category: Ptb domain]]		[[Category: Ptb domain]]
-	[[Category: ~~Riken structural genomics/proteomics initiative~~]]	+	[[Category: Structural genomic]]
	[[Category: Rsgi]]		[[Category: Rsgi]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]
	[[Category: Snt-2]]		[[Category: Snt-2]]
-	[[Category: Structural genomic]]

OCA at 04:28, 13 February 2014

OCA — Thu, 13 Feb 2014 04:28:31 GMT

←Older revision		Revision as of 04:28, 13 February 2014
Line 4:		Line 4:

	==Function==		==Function==
-	[[http://www.uniprot.org/uniprot/FRS3_HUMAN FRS3_HUMAN]] Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and nuclear translocation of ERK2.<ref>PMID:15094036</ref>	+	[[http://www.uniprot.org/uniprot/FRS3_HUMAN FRS3_HUMAN]] Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and nuclear translocation of ERK2.<ref>PMID:15094036</ref>

	==About this Structure==		==About this Structure==
-	[[2kuq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KUQ OCA].	+	[[2kuq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KUQ OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:020454865</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:020454865</ref><references group="xtra"/><references/>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Harada, T.]]		[[Category: Harada, T.]]
	[[Category: Kigawa, T.]]		[[Category: Kigawa, T.]]

OCA at 01:19, 25 March 2013

OCA — Mon, 25 Mar 2013 01:19:48 GMT

←Older revision		Revision as of 01:19, 25 March 2013
Line 1:		Line 1:
-	[[Image:2kuq.png\|left\|200px]]
-
	{{STRUCTURE_2kuq\| PDB=2kuq \| SCENE= }}		{{STRUCTURE_2kuq\| PDB=2kuq \| SCENE= }}
-
	===Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK===		===Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK===
		+	{{ABSTRACT_PUBMED_20454865}}

-	~~{{ABSTRACT_PUBMED_20454865}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/FRS3_HUMAN FRS3_HUMAN]] Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and nuclear translocation of ERK2.<ref>PMID:15094036</ref>

	==About this Structure==		==About this Structure==
Line 11:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:020454865</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020454865</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Harada, T.]]		[[Category: Harada, T.]]

OCA: Protected "2kuq" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 11:43:19 GMT

Protected "2kuq" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:43, 7 January 2013

OCA at 11:43, 7 January 2013

OCA — Mon, 07 Jan 2013 11:43:14 GMT

←Older revision		Revision as of 11:43, 7 January 2013
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:2kuq.png\|left\|200px]]
-	[[Image:2kuq.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2kuq", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2kuq\| PDB=2kuq \| SCENE= }}		{{STRUCTURE_2kuq\| PDB=2kuq \| SCENE= }}

	===Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK===		===Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20454865}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20454865 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20454865}}		{{ABSTRACT_PUBMED_20454865}}

	==About this Structure==		==About this Structure==
-	~~2KUQ~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens~~,_synthetic,_synthetic~~ Homo sapiens~~, synthetic, synthetic~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KUQ OCA].	+	[[2kuq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KUQ OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20454865~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020454865</ref><references group="xtra"/>
-	[[Category: Homo sapiens~~, synthetic, synthetic~~]]	+	[[Category: Homo sapiens]]
	[[Category: Harada, T.]]		[[Category: Harada, T.]]
	[[Category: Kigawa, T.]]		[[Category: Kigawa, T.]]
Line 42:		Line 30:
	[[Category: Snt-2]]		[[Category: Snt-2]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 26 08:27:26 2010''