2kw0 - Revision history

OCA at 06:49, 1 May 2024

OCA — Wed, 01 May 2024 06:49:19 GMT

←Older revision		Revision as of 06:49, 1 May 2024
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [https://pdbe.org/2kw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2kw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kw0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [https://pdbe.org/2kw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2kw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kw0 ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In the Gram-negative bacterium of Escherichia coli, eight genes organized as a ccm operon (ccmABCDEFGH) are involved in the maturation of c-type cytochromes. The proteins encoded by the last three genes ccmFGH are believed to form a lyase complex functioning in the reduction of apocytochrome c and haem attachment. Among them, CcmH is a membrane-associated protein; its N-terminus is a catalytic domain with the active CXXC motif and the C-terminus is predicted as a TPR-like domain with unknown function. By using SCAM (scanning cysteine accessibility mutagenesis) and Gaussia luciferase fusion assays, we provide experimental evidence for the entire topological structure of E. coli CcmH. The mature CcmH is a periplasm-resident oxidoreductase anchored to the inner membrane by two transmembrane segments. Both N- and C-terminal domains are located and function in the periplasmic compartment. Moreover, the N-terminal domain forms a monomer in solution, while the C-terminal domain is a compact fold with helical structures. The NMR solution structure of the catalytic domain in reduced form exhibits mainly a three-helix bundle, providing further information for the redox mechanism. The redox potential suggests that CcmH exhibits a strong reductase that may function in the last step of reduction of apocytochrome c for haem attachment.
-
-	Biochemical properties and catalytic domain structure of the CcmH protein from Escherichia coli.,Zheng XM, Hong J, Li HY, Lin DH, Hu HY Biochim Biophys Acta. 2012 Dec;1824(12):1394-400. doi:, 10.1016/j.bbapap.2012.06.017. Epub 2012 Jul 10. PMID:22789558<ref>PMID:22789558</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2kw0" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:28, 6 September 2023

OCA — Wed, 06 Sep 2023 10:28:23 GMT

←Older revision		Revision as of 10:28, 6 September 2023
Line 1:		Line 1:

	==Solution structure of N-terminal domain of CcmH from Escherichia.coli==		==Solution structure of N-terminal domain of CcmH from Escherichia.coli==
-	<StructureSection load='2kw0' size='340' side='right'caption='[[2kw0~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2kw0' size='340' side='right'caption='[[2kw0]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecobb Ecobb~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21 Escherichia coli BL21]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>b2194, B21_02080, ccmH, JW2182, yejP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~511693 ECOBB])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [https://pdbe.org/2kw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2kw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kw0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [https://pdbe.org/2kw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2kw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kw0 ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecobb~~]]	+	[[Category: Escherichia coli BL21]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hong, J]]	+	[[Category: Hong J]]
-	[[Category: Hu, H]]	+	[[Category: Hu H]]
-	[[Category: Lin, D]]	+	[[Category: Lin D]]
-	[[Category: Zheng, X]]	+	[[Category: Zheng X]]
-	~~[[Category: Cytochrome c maturation]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:10, 1 December 2021

OCA — Wed, 01 Dec 2021 07:10:15 GMT

←Older revision		Revision as of 07:10, 1 December 2021
Line 3:		Line 3:
	<StructureSection load='2kw0' size='340' side='right'caption='[[2kw0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kw0' size='340' side='right'caption='[[2kw0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecobb Ecobb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecobb Ecobb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2194, B21_02080, ccmH, JW2182, yejP ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511693 ECOBB])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2194, B21_02080, ccmH, JW2182, yejP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511693 ECOBB])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [~~http~~://pdbe.org/2kw0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2kw0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2kw0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [https://pdbe.org/2kw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2kw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kw0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:45, 26 February 2020

OCA — Wed, 26 Feb 2020 09:45:16 GMT

←Older revision		Revision as of 09:45, 26 February 2020
Line 1:		Line 1:

	==Solution structure of N-terminal domain of CcmH from Escherichia.coli==		==Solution structure of N-terminal domain of CcmH from Escherichia.coli==
-	<StructureSection load='2kw0' size='340' side='right' caption='[[2kw0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2kw0' size='340' side='right'caption='[[2kw0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecobb Ecobb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecobb Ecobb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Ecobb]]		[[Category: Ecobb]]
		+	[[Category: Large Structures]]
	[[Category: Hong, J]]		[[Category: Hong, J]]
	[[Category: Hu, H]]		[[Category: Hu, H]]

OCA at 07:46, 25 July 2018

OCA — Wed, 25 Jul 2018 07:46:36 GMT

←Older revision		Revision as of 07:46, 25 July 2018
Line 1:		Line 1:
		+
	==Solution structure of N-terminal domain of CcmH from Escherichia.coli==		==Solution structure of N-terminal domain of CcmH from Escherichia.coli==
	<StructureSection load='2kw0' size='340' side='right' caption='[[2kw0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kw0' size='340' side='right' caption='[[2kw0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecobb Ecobb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecobb Ecobb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2194, B21_02080, ccmH, JW2182, yejP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511693 ECOBB])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2194, B21_02080, ccmH, JW2182, yejP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511693 ECOBB])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [http://pdbe.org/2kw0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [http://www.ebi.ac.uk/pdbsum/2kw0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [http://pdbe.org/2kw0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [http://www.ebi.ac.uk/pdbsum/2kw0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kw0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:39, 10 September 2015

OCA — Thu, 10 Sep 2015 14:39:31 GMT

←Older revision		Revision as of 14:39, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2kw0' size='340' side='right' caption='[[2kw0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2kw0' size='340' side='right' caption='[[2kw0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_bl21 Escherichia coli bl21~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecobb Ecobb]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2194, B21_02080, ccmH, JW2182, yejP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511693 ~~Escherichia coli BL21~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2194, B21_02080, ccmH, JW2182, yejP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511693 ECOBB])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [http://www.ebi.ac.uk/pdbsum/2kw0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [http://pdbe.org/2kw0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [http://www.ebi.ac.uk/pdbsum/2kw0 PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2kw0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia coli bl21~~]]	+	[[Category: Ecobb]]
	[[Category: Hong, J]]		[[Category: Hong, J]]
	[[Category: Hu, H]]		[[Category: Hu, H]]

OCA at 16:09, 19 January 2015

OCA — Mon, 19 Jan 2015 16:09:24 GMT

←Older revision		Revision as of 16:09, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_bl21 Escherichia coli bl21]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_bl21 Escherichia coli bl21]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2194, B21_02080, ccmH, JW2182, yejP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511693 Escherichia coli BL21])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2194, B21_02080, ccmH, JW2182, yejP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511693 Escherichia coli BL21])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [http://www.ebi.ac.uk/pdbsum/2kw0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [http://www.ebi.ac.uk/pdbsum/2kw0 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli bl21]]		[[Category: Escherichia coli bl21]]
-	[[Category: Hong, J.]]	+	[[Category: Hong, J]]
-	[[Category: Hu, H.]]	+	[[Category: Hu, H]]
-	[[Category: Lin, D.]]	+	[[Category: Lin, D]]
-	[[Category: Zheng, X.]]	+	[[Category: Zheng, X]]
	[[Category: Cytochrome c maturation]]		[[Category: Cytochrome c maturation]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 05:28, 29 September 2014

OCA — Mon, 29 Sep 2014 05:28:22 GMT

←Older revision		Revision as of 05:28, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2kw0.~~png~~\|~~left\|200px~~]]	+	==Solution structure of N-terminal domain of CcmH from Escherichia.coli==
		+	<StructureSection load='2kw0' size='340' side='right' caption='[[2kw0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_bl21 Escherichia coli bl21]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b2194, B21_02080, ccmH, JW2182, yejP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511693 Escherichia coli BL21])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [http://www.ebi.ac.uk/pdbsum/2kw0 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In the Gram-negative bacterium of Escherichia coli, eight genes organized as a ccm operon (ccmABCDEFGH) are involved in the maturation of c-type cytochromes. The proteins encoded by the last three genes ccmFGH are believed to form a lyase complex functioning in the reduction of apocytochrome c and haem attachment. Among them, CcmH is a membrane-associated protein; its N-terminus is a catalytic domain with the active CXXC motif and the C-terminus is predicted as a TPR-like domain with unknown function. By using SCAM (scanning cysteine accessibility mutagenesis) and Gaussia luciferase fusion assays, we provide experimental evidence for the entire topological structure of E. coli CcmH. The mature CcmH is a periplasm-resident oxidoreductase anchored to the inner membrane by two transmembrane segments. Both N- and C-terminal domains are located and function in the periplasmic compartment. Moreover, the N-terminal domain forms a monomer in solution, while the C-terminal domain is a compact fold with helical structures. The NMR solution structure of the catalytic domain in reduced form exhibits mainly a three-helix bundle, providing further information for the redox mechanism. The redox potential suggests that CcmH exhibits a strong reductase that may function in the last step of reduction of apocytochrome c for haem attachment.

-	~~{{STRUCTURE_2kw0\| PDB=2kw0 \| SCENE= }}~~	+	Biochemical properties and catalytic domain structure of the CcmH protein from Escherichia coli.,Zheng XM, Hong J, Li HY, Lin DH, Hu HY Biochim Biophys Acta. 2012 Dec;1824(12):1394-400. doi:, 10.1016/j.bbapap.2012.06.017. Epub 2012 Jul 10. PMID:22789558<ref>PMID:22789558</ref>

-	~~===Solution structure~~ of ~~N-terminal domain~~ of ~~CcmH from Escherichia~~.~~coli===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	==~~About this Structure~~==	+	<references/>
-	~~[[2kw0]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Escherichia_coli_bl21 Escherichia coli bl21]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA].~~	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli bl21]]		[[Category: Escherichia coli bl21]]
	[[Category: Hong, J.]]		[[Category: Hong, J.]]

OCA at 13:42, 8 November 2012

OCA — Thu, 08 Nov 2012 13:42:14 GMT

←Older revision		Revision as of 13:42, 8 November 2012
Line 1:		Line 1:
-	[[Image:2kw0.~~jpg~~\|left\|200px]]	+	[[Image:2kw0.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2kw0", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2kw0\| PDB=2kw0 \| SCENE= }}		{{STRUCTURE_2kw0\| PDB=2kw0 \| SCENE= }}

Line 13:		Line 7:

	==About this Structure==		==About this Structure==
-	[[2kw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli~~ Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA].	+	[[2kw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_bl21 Escherichia coli bl21]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA].
-	[[Category: Escherichia coli]]	+	[[Category: Escherichia coli bl21]]
	[[Category: Hong, J.]]		[[Category: Hong, J.]]
	[[Category: Hu, H.]]		[[Category: Hu, H.]]

OCA: Protected "2kw0" [edit=sysop:move=sysop]

OCA — Wed, 13 Jul 2011 07:43:31 GMT

Protected "2kw0" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:43, 13 July 2011