2l0q - Revision history

OCA at 06:51, 1 May 2024

OCA — Wed, 01 May 2024 06:51:12 GMT

←Older revision		Revision as of 06:51, 1 May 2024
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	==NMR Solution Structure of Vibrio harveyi Acyl Carrier Protein (ACP)==		==NMR Solution Structure of Vibrio harveyi Acyl Carrier Protein (ACP)==
-	<StructureSection load='2l0q' size='340' side='right'caption='[[2l0q~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2l0q' size='340' side='right'caption='[[2l0q]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l0q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Vibh1 Vibh1~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L0Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l0q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_harveyi_1DA3 Vibrio harveyi 1DA3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L0Q FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>acpP, VME_27770 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~673519 VIBH1])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0q OCA], [https://pdbe.org/2l0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l0q RCSB], [https://www.ebi.ac.uk/pdbsum/2l0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0q OCA], [https://pdbe.org/2l0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l0q RCSB], [https://www.ebi.ac.uk/pdbsum/2l0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~D0XCG4_VIBHA D0XCG4_VIBHA]~~] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217][RuleBase:RU003545]	+	[https://www.uniprot.org/uniprot/D0XCG4_VIBH1 D0XCG4_VIBH1] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217][RuleBase:RU003545]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2l0q ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2l0q ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Bacterial acyl carrier protein (ACP) is a highly anionic, 9 kDa protein that functions as a cofactor protein in fatty acid biosynthesis. Escherichia coli ACP is folded at neutral pH and in the absence of divalent cations, while Vibrio harveyi ACP, which is very similar at 86% sequence identity, is unfolded under the same conditions. V. harveyi ACP adopts a folded conformation upon the addition of divalent cations such as Ca(2+) and Mg(2+) and a mutant, A75H, was previously identified that restores the folded conformation at pH 7 in the absence of divalent cations. In this study we sought to understand the unique folding behavior of V. harveyi ACP using NMR spectroscopy and biophysical methods. The NMR solution structure of V. harveyi ACP A75H displays the canonical ACP structure with four helices surrounding a hydrophobic core, with a narrow pocket closed off from the solvent to house the acyl chain. His-75, which is charged at neutral pH, participates in a stacking interaction with Tyr-71 in the far C-terminal end of helix IV. pH titrations and the electrostatic profile of ACP suggest that V. harveyi ACP is destabilized by anionic charge repulsion around helix II that can be partially neutralized by His-75 and is further reduced by divalent cation binding. This is supported by differential scanning calorimetry data which indicate that calcium binding further increases the melting temperature of V. harveyi ACP A75H by approximately 20 degrees C. Divalent cation binding does not alter ACP dynamics on the ps-ns timescale as determined by (15)N NMR relaxation experiments, however, it clearly stabilizes the protein fold as observed by hydrogen-deuterium exchange studies. Finally, we demonstrate that the E. coli ACP H75A mutant is similarly unfolded as wild-type V. harveyi ACP, further stressing the importance of this particular residue for proper protein folding.
-
-	NMR solution structure and biophysical characterization of Vibrio harveyi acyl carrier protein A75H: effects of divalent metal ions.,Chan DI, Chu BC, Lau CK, Hunter HN, Byers DM, Vogel HJ J Biol Chem. 2010 Oct 1;285(40):30558-66. Epub 2010 Jul 21. PMID:20659901<ref>PMID:20659901</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2l0q" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Acyl carrier protein 3D structures\|Acyl carrier protein 3D structures]]		*[[Acyl carrier protein 3D structures\|Acyl carrier protein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Vibh1~~]]	+	[[Category: Vibrio harveyi 1DA3]]
-	[[Category: Byers~~, D M~~]]	+	[[Category: Byers DM]]
-	[[Category: Chan~~, D I~~]]	+	[[Category: Chan DI]]
-	[[Category: Chu~~, B C.H~~]]	+	[[Category: Chu BCH]]
-	[[Category: Hunter~~, H N~~]]	+	[[Category: Hunter HN]]
-	[[Category: Lau~~, C K.Y~~]]	+	[[Category: Lau CKY]]
-	[[Category: Vogel~~, H J]]~~	+	[[Category: Vogel HJ]]
-	~~[[Category: Acyl carrier protein]]~~	+
-	~~[[Category: Acyl chain binding]]~~	+
-	~~[[Category: Fatty acid biosynthesis]]~~	+
-	~~[[Category: Lipid binding protein~~]]	+

OCA at 07:34, 14 April 2021

OCA — Wed, 14 Apr 2021 07:34:34 GMT

←Older revision		Revision as of 07:34, 14 April 2021
Line 1:		Line 1:

	==NMR Solution Structure of Vibrio harveyi Acyl Carrier Protein (ACP)==		==NMR Solution Structure of Vibrio harveyi Acyl Carrier Protein (ACP)==
-	<StructureSection load='2l0q' size='340' side='right' caption='[[2l0q]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2l0q' size='340' side='right'caption='[[2l0q]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l0q]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Vibh1 Vibh1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2L0Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l0q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibh1 Vibh1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L0Q FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP, VME_27770 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=673519 VIBH1])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP, VME_27770 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=673519 VIBH1])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2l0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0q OCA], [~~http~~://pdbe.org/2l0q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2l0q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2l0q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0q OCA], [https://pdbe.org/2l0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l0q RCSB], [https://www.ebi.ac.uk/pdbsum/2l0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/D0XCG4_VIBHA D0XCG4_VIBHA]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217][RuleBase:RU003545]	+	[[https://www.uniprot.org/uniprot/D0XCG4_VIBHA D0XCG4_VIBHA]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217][RuleBase:RU003545]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Acyl carrier protein\|Acyl carrier protein]]	+	*[[Acyl carrier protein 3D structures\|Acyl carrier protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Vibh1]]		[[Category: Vibh1]]
	[[Category: Byers, D M]]		[[Category: Byers, D M]]

OCA at 08:14, 25 July 2018

OCA — Wed, 25 Jul 2018 08:14:25 GMT

←Older revision		Revision as of 08:14, 25 July 2018
Line 1:		Line 1:
		+
	==NMR Solution Structure of Vibrio harveyi Acyl Carrier Protein (ACP)==		==NMR Solution Structure of Vibrio harveyi Acyl Carrier Protein (ACP)==
	<StructureSection load='2l0q' size='340' side='right' caption='[[2l0q]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2l0q' size='340' side='right' caption='[[2l0q]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l0q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Vibrio_harveyi_1da3 Vibrio harveyi 1da3~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L0Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l0q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibh1 Vibh1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L0Q FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP, VME_27770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=673519 ~~Vibrio harveyi 1DA3~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP, VME_27770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=673519 VIBH1])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0q OCA], [http://pdbe.org/2l0q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l0q RCSB], [http://www.ebi.ac.uk/pdbsum/2l0q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0q OCA], [http://pdbe.org/2l0q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l0q RCSB], [http://www.ebi.ac.uk/pdbsum/2l0q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l0q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l0/2l0q_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l0/2l0q_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2l0q ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio harveyi 1da3~~]]	+	[[Category: Vibh1]]
	[[Category: Byers, D M]]		[[Category: Byers, D M]]
	[[Category: Chan, D I]]		[[Category: Chan, D I]]

OCA at 07:52, 10 September 2015

OCA — Thu, 10 Sep 2015 07:52:19 GMT

←Older revision		Revision as of 07:52, 10 September 2015
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	<table><tr><td colspan='2'>[[2l0q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi_1da3 Vibrio harveyi 1da3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L0Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[2l0q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi_1da3 Vibrio harveyi 1da3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L0Q FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP, VME_27770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=673519 Vibrio harveyi 1DA3])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP, VME_27770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=673519 Vibrio harveyi 1DA3])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l0q RCSB], [http://www.ebi.ac.uk/pdbsum/2l0q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0q OCA], [http://pdbe.org/2l0q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l0q RCSB], [http://www.ebi.ac.uk/pdbsum/2l0q PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2l0q" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 20:37, 24 December 2014

OCA — Wed, 24 Dec 2014 20:37:09 GMT

←Older revision		Revision as of 20:37, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l0q RCSB], [http://www.ebi.ac.uk/pdbsum/2l0q PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l0q RCSB], [http://www.ebi.ac.uk/pdbsum/2l0q PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/D0XCG4_VIBHA D0XCG4_VIBHA]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217][RuleBase:RU003545]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Acyl carrier protein\|Acyl carrier protein]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Vibrio harveyi 1da3]]		[[Category: Vibrio harveyi 1da3]]
-	[[Category: Byers, D M.]]	+	[[Category: Byers, D M]]
-	[[Category: Chan, D I.]]	+	[[Category: Chan, D I]]
-	[[Category: Chu, B C.H.]]	+	[[Category: Chu, B C.H]]
-	[[Category: Hunter, H N.]]	+	[[Category: Hunter, H N]]
-	[[Category: Lau, C K.Y.]]	+	[[Category: Lau, C K.Y]]
-	[[Category: Vogel, H J.]]	+	[[Category: Vogel, H J]]
	[[Category: Acyl carrier protein]]		[[Category: Acyl carrier protein]]
	[[Category: Acyl chain binding]]		[[Category: Acyl chain binding]]
	[[Category: Fatty acid biosynthesis]]		[[Category: Fatty acid biosynthesis]]
	[[Category: Lipid binding protein]]		[[Category: Lipid binding protein]]

OCA at 11:17, 20 October 2014

OCA — Mon, 20 Oct 2014 11:17:20 GMT

←Older revision		Revision as of 11:17, 20 October 2014
Line 1:		Line 1:
-	[[~~Image~~:2l0q.~~png\|left~~\|200px]]	+	==NMR Solution Structure of Vibrio harveyi Acyl Carrier Protein (ACP)==
		+	<StructureSection load='2l0q' size='340' side='right' caption='[[2l0q]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2l0q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi_1da3 Vibrio harveyi 1da3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L0Q FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acpP, VME_27770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=673519 Vibrio harveyi 1DA3])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l0q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l0q RCSB], [http://www.ebi.ac.uk/pdbsum/2l0q PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l0/2l0q_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial acyl carrier protein (ACP) is a highly anionic, 9 kDa protein that functions as a cofactor protein in fatty acid biosynthesis. Escherichia coli ACP is folded at neutral pH and in the absence of divalent cations, while Vibrio harveyi ACP, which is very similar at 86% sequence identity, is unfolded under the same conditions. V. harveyi ACP adopts a folded conformation upon the addition of divalent cations such as Ca(2+) and Mg(2+) and a mutant, A75H, was previously identified that restores the folded conformation at pH 7 in the absence of divalent cations. In this study we sought to understand the unique folding behavior of V. harveyi ACP using NMR spectroscopy and biophysical methods. The NMR solution structure of V. harveyi ACP A75H displays the canonical ACP structure with four helices surrounding a hydrophobic core, with a narrow pocket closed off from the solvent to house the acyl chain. His-75, which is charged at neutral pH, participates in a stacking interaction with Tyr-71 in the far C-terminal end of helix IV. pH titrations and the electrostatic profile of ACP suggest that V. harveyi ACP is destabilized by anionic charge repulsion around helix II that can be partially neutralized by His-75 and is further reduced by divalent cation binding. This is supported by differential scanning calorimetry data which indicate that calcium binding further increases the melting temperature of V. harveyi ACP A75H by approximately 20 degrees C. Divalent cation binding does not alter ACP dynamics on the ps-ns timescale as determined by (15)N NMR relaxation experiments, however, it clearly stabilizes the protein fold as observed by hydrogen-deuterium exchange studies. Finally, we demonstrate that the E. coli ACP H75A mutant is similarly unfolded as wild-type V. harveyi ACP, further stressing the importance of this particular residue for proper protein folding.

-	~~{{STRUCTURE_2l0q\| PDB=2l0q \| SCENE= }}~~	+	NMR solution structure and biophysical characterization of Vibrio harveyi acyl carrier protein A75H: effects of divalent metal ions.,Chan DI, Chu BC, Lau CK, Hunter HN, Byers DM, Vogel HJ J Biol Chem. 2010 Oct 1;285(40):30558-66. Epub 2010 Jul 21. PMID:20659901<ref>PMID:20659901</ref>

-	~~===NMR Solution Structure~~ of ~~Vibrio harveyi Acyl Carrier Protein (ACP)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20659901}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2l0q]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Vibrio_harveyi_1da3 Vibrio harveyi 1da3]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:020659901<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Vibrio harveyi 1da3]]		[[Category: Vibrio harveyi 1da3]]
	[[Category: Byers, D M.]]		[[Category: Byers, D M.]]

OCA: Protected "2l0q" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 13:46:14 GMT

Protected "2l0q" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:46, 7 January 2013

OCA at 13:46, 7 January 2013

OCA — Mon, 07 Jan 2013 13:46:12 GMT

←Older revision		Revision as of 13:46, 7 January 2013
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-	{{Seed}}
	[[Image:2l0q.png\|left\|200px]]		[[Image:2l0q.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2l0q", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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-	-->
	{{STRUCTURE_2l0q\| PDB=2l0q \| SCENE= }}		{{STRUCTURE_2l0q\| PDB=2l0q \| SCENE= }}

	===NMR Solution Structure of Vibrio harveyi Acyl Carrier Protein (ACP)===		===NMR Solution Structure of Vibrio harveyi Acyl Carrier Protein (ACP)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20659901}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20659901 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_20659901}}		{{ABSTRACT_PUBMED_20659901}}

	==About this Structure==		==About this Structure==
-	~~2L0Q~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi_1da3 Vibrio harveyi 1da3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA].	+	[[2l0q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi_1da3 Vibrio harveyi 1da3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20659901~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020659901</ref><references group="xtra"/>
	[[Category: Vibrio harveyi 1da3]]		[[Category: Vibrio harveyi 1da3]]
	[[Category: Byers, D M.]]		[[Category: Byers, D M.]]
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	[[Category: Fatty acid biosynthesis]]		[[Category: Fatty acid biosynthesis]]
	[[Category: Lipid binding protein]]		[[Category: Lipid binding protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 24 12:56:05 2010''

OCA at 09:44, 24 November 2010

OCA — Wed, 24 Nov 2010 09:44:57 GMT

←Older revision		Revision as of 09:44, 24 November 2010
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	{{Seed}}		{{Seed}}
-	[[Image:2l0q.~~jpg~~\|left\|200px]]	+	[[Image:2l0q.png\|left\|200px]]

	<!--		<!--
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	===NMR Solution Structure of Vibrio harveyi Acyl Carrier Protein (ACP)===		===NMR Solution Structure of Vibrio harveyi Acyl Carrier Protein (ACP)===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20659901}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20659901 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20659901}}

	==About this Structure==		==About this Structure==
	2L0Q is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi_1da3 Vibrio harveyi 1da3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA].		2L0Q is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi_1da3 Vibrio harveyi 1da3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L0Q OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20659901</ref><references group="xtra"/>
	[[Category: Vibrio harveyi 1da3]]		[[Category: Vibrio harveyi 1da3]]
	[[Category: Byers, D M.]]		[[Category: Byers, D M.]]
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	[[Category: Lipid binding protein]]		[[Category: Lipid binding protein]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jul 21 10~~:13:14 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 24 12:56:05 2010''

OCA: New page: {{Seed}} 200px