2l62 - Revision history

OCA at 06:53, 1 May 2024

OCA — Wed, 01 May 2024 06:53:34 GMT

←Older revision		Revision as of 06:53, 1 May 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.		[https://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Metallothioneins (MTs) are small cysteine-rich proteins coordinating various transition metal ions, including Zn(II), Cd(II), and Cu(I). MTs are ubiquitously present in all phyla, indicating a successful molecular concept for metal ion binding in all organisms. The plant MT E(c)-1 from Triticum aestivum, common bread wheat, is a Zn(II)-binding protein that comprises two domains and binds up to six metal ions. The structure of the C-terminal four metal ion binding beta(E) domain was recently described. Here we present the structure of the N-terminal second domain, gamma-E(c)-1, determined by NMR spectroscopy. The gamma-E(c)-1 domain enfolds an M (2) (II) Cys(6) cluster and was characterized as part of the full-length Zn(6)E(c)-1 protein as well as in the form of the separately expressed domain, both in the Zn(II)-containing isoform and the Cd(II)-containing isoform. Extended X-ray absorption fine structure analysis of Zn(2)gamma-E(c)-1 clearly shows the presence of a ZnS(4) coordination sphere with average Zn-S distances of 2.33 A. (113)Cd NMR experiments were used to identify the M(II)-Cys connectivity pattern, and revealed two putative metal cluster conformations. In addition, the general metal ion coordination abilities of gamma-E(c)-1 were probed with Cd(II) binding experiments as well as by pH titrations of the Zn(II) and Cd(II) forms, the latter suggesting an interaction of the gamma domain and the beta(E) domain within the full-length protein.
-
-	Protein and metal cluster structure of the wheat metallothionein domain gamma-E(c)-1: the second part of the puzzle.,Loebus J, Peroza EA, Bluthgen N, Fox T, Meyer-Klaucke W, Zerbe O, Freisinger E J Biol Inorg Chem. 2011 Mar 25. PMID:21437709<ref>PMID:21437709</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2l62" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:08, 26 July 2023

OCA — Wed, 26 Jul 2023 13:08:43 GMT

←Older revision		Revision as of 13:08, 26 July 2023
Line 1:		Line 1:

	==Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.==		==Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.==
-	<StructureSection load='2l62' size='340' side='right'caption='[[2l62~~]], [[NMR_Ensembles_of_Models \| 40 NMR models~~]]' scene=''>	+	<StructureSection load='2l62' size='340' side='right'caption='[[2l62]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l62]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Canadian_hard_winter_wheat Canadian hard winter wheat~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L62 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l62]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L62 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l62 OCA], [https://pdbe.org/2l62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l62 RCSB], [https://www.ebi.ac.uk/pdbsum/2l62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l62 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l62 OCA], [https://pdbe.org/2l62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l62 RCSB], [https://www.ebi.ac.uk/pdbsum/2l62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l62 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT]] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.	+	[https://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Canadian hard winter wheat]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bluethgen, N]]	+	[[Category: Triticum aestivum]]
-	[[Category: Fox, T]]	+	[[Category: Bluethgen N]]
-	[[Category: Freisinger, E]]	+	[[Category: Fox T]]
-	[[Category: Loebus, J]]	+	[[Category: Freisinger E]]
-	[[Category: Meyer-Klaucke, W]]	+	[[Category: Loebus J]]
-	[[Category: Peroza~~, E A~~]]	+	[[Category: Meyer-Klaucke W]]
-	[[Category: Zerbe, O]]	+	[[Category: Peroza EA]]
-	~~[[Category: Metal binding protein]]~~	+	[[Category: Zerbe O]]
-	~~[[Category: Metal-thiolate cluster]]~~	+
-	~~[[Category: Metallothionein]]~~	+
-	~~[[Category: Wheat ec-1]]~~	+
-	~~[[Category: Zn binding~~]]	+

OCA at 20:47, 20 October 2021

OCA — Wed, 20 Oct 2021 20:47:50 GMT

←Older revision		Revision as of 20:47, 20 October 2021
Line 3:		Line 3:
	<StructureSection load='2l62' size='340' side='right'caption='[[2l62]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>		<StructureSection load='2l62' size='340' side='right'caption='[[2l62]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l62]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2L62 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l62]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L62 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l62 OCA], [~~http~~://pdbe.org/2l62 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2l62 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2l62 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2l62 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l62 OCA], [https://pdbe.org/2l62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l62 RCSB], [https://www.ebi.ac.uk/pdbsum/2l62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l62 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT]] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.	+	[[https://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT]] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 19:34, 11 December 2019

OCA — Wed, 11 Dec 2019 19:34:05 GMT

←Older revision		Revision as of 19:34, 11 December 2019
Line 1:		Line 1:

	==Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.==		==Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.==
-	<StructureSection load='2l62' size='340' side='right' caption='[[2l62]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>	+	<StructureSection load='2l62' size='340' side='right'caption='[[2l62]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2l62]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L62 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2l62]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L62 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Canadian hard winter wheat]]		[[Category: Canadian hard winter wheat]]
		+	[[Category: Large Structures]]
	[[Category: Bluethgen, N]]		[[Category: Bluethgen, N]]
	[[Category: Fox, T]]		[[Category: Fox, T]]

OCA at 07:08, 29 November 2017

OCA — Wed, 29 Nov 2017 07:08:13 GMT

←Older revision		Revision as of 07:08, 29 November 2017
Line 1:		Line 1:
		+
	==Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.==		==Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.==
	<StructureSection load='2l62' size='340' side='right' caption='[[2l62]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>		<StructureSection load='2l62' size='340' side='right' caption='[[2l62]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2l62]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L62 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2l62]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L62 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l62 OCA], [http://pdbe.org/2l62 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l62 RCSB], [http://www.ebi.ac.uk/pdbsum/2l62 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l62 OCA], [http://pdbe.org/2l62 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l62 RCSB], [http://www.ebi.ac.uk/pdbsum/2l62 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l62 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 16:59, 11 September 2015

OCA — Fri, 11 Sep 2015 16:59:59 GMT

←Older revision		Revision as of 16:59, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2l62' size='340' side='right' caption='[[2l62]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>		<StructureSection load='2l62' size='340' side='right' caption='[[2l62]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l62]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Triticum_aestivum Triticum aestivum~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L62 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l62]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L62 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l62 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l62 RCSB], [http://www.ebi.ac.uk/pdbsum/2l62 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l62 OCA], [http://pdbe.org/2l62 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l62 RCSB], [http://www.ebi.ac.uk/pdbsum/2l62 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2l62" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Triticum aestivum~~]]	+	[[Category: Canadian hard winter wheat]]
	[[Category: Bluethgen, N]]		[[Category: Bluethgen, N]]
	[[Category: Fox, T]]		[[Category: Fox, T]]

OCA at 19:19, 25 December 2014

OCA — Thu, 25 Dec 2014 19:19:58 GMT

←Older revision		Revision as of 19:19, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l62 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l62 RCSB], [http://www.ebi.ac.uk/pdbsum/2l62 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l62 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l62 RCSB], [http://www.ebi.ac.uk/pdbsum/2l62 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT]] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:32, 18 December 2014

OCA — Thu, 18 Dec 2014 12:32:11 GMT

←Older revision		Revision as of 12:32, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2l62\| PDB=2l62 \| SCENE= }}~~	+	==Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.==
-	===Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.===	+	<StructureSection load='2l62' size='340' side='right' caption='[[2l62]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
-	~~{{ABSTRACT_PUBMED_21437709}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2l62]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L62 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l62 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l62 RCSB], [http://www.ebi.ac.uk/pdbsum/2l62 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metallothioneins (MTs) are small cysteine-rich proteins coordinating various transition metal ions, including Zn(II), Cd(II), and Cu(I). MTs are ubiquitously present in all phyla, indicating a successful molecular concept for metal ion binding in all organisms. The plant MT E(c)-1 from Triticum aestivum, common bread wheat, is a Zn(II)-binding protein that comprises two domains and binds up to six metal ions. The structure of the C-terminal four metal ion binding beta(E) domain was recently described. Here we present the structure of the N-terminal second domain, gamma-E(c)-1, determined by NMR spectroscopy. The gamma-E(c)-1 domain enfolds an M (2) (II) Cys(6) cluster and was characterized as part of the full-length Zn(6)E(c)-1 protein as well as in the form of the separately expressed domain, both in the Zn(II)-containing isoform and the Cd(II)-containing isoform. Extended X-ray absorption fine structure analysis of Zn(2)gamma-E(c)-1 clearly shows the presence of a ZnS(4) coordination sphere with average Zn-S distances of 2.33 A. (113)Cd NMR experiments were used to identify the M(II)-Cys connectivity pattern, and revealed two putative metal cluster conformations. In addition, the general metal ion coordination abilities of gamma-E(c)-1 were probed with Cd(II) binding experiments as well as by pH titrations of the Zn(II) and Cd(II) forms, the latter suggesting an interaction of the gamma domain and the beta(E) domain within the full-length protein.

-	~~==Function==~~	+	Protein and metal cluster structure of the wheat metallothionein domain gamma-E(c)-1: the second part of the puzzle.,Loebus J, Peroza EA, Bluthgen N, Fox T, Meyer-Klaucke W, Zerbe O, Freisinger E J Biol Inorg Chem. 2011 Mar 25. PMID:21437709<ref>PMID:21437709</ref>
-	~~[[http~~:~~//www~~.~~uniprot~~.~~org~~/~~uniprot/EC1_WHEAT EC1_WHEAT]] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.~~	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[2l62]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Triticum_aestivum Triticum aestivum]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:021437709</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Triticum aestivum]]		[[Category: Triticum aestivum]]
-	[[Category: Bluethgen, N.]]	+	[[Category: Bluethgen, N]]
-	[[Category: Fox, T.]]	+	[[Category: Fox, T]]
-	[[Category: Freisinger, E.]]	+	[[Category: Freisinger, E]]
-	[[Category: Loebus, J.]]	+	[[Category: Loebus, J]]
-	[[Category: Meyer-Klaucke, W.]]	+	[[Category: Meyer-Klaucke, W]]
-	[[Category: Peroza, E A.]]	+	[[Category: Peroza, E A]]
-	[[Category: Zerbe, O.]]	+	[[Category: Zerbe, O]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]
	[[Category: Metal-thiolate cluster]]		[[Category: Metal-thiolate cluster]]

OCA at 09:22, 27 March 2013

OCA — Wed, 27 Mar 2013 09:22:50 GMT

←Older revision		Revision as of 09:22, 27 March 2013
Line 1:		Line 1:
-	[[Image:2l62.jpg\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_2l62", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2l62\| PDB=2l62 \| SCENE= }}		{{STRUCTURE_2l62\| PDB=2l62 \| SCENE= }}
-
	===Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.===		===Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.===
		+	{{ABSTRACT_PUBMED_21437709}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/EC1_WHEAT EC1_WHEAT]] Binds 5 molecules of zinc. May have a role in Zn(2+) homeostasis during embryogenesis.
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21437709}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov~~)~~, where 21437709 is the PubMed ID number~~.	+
-	~~-->~~	+
-	~~{{ABSTRACT_PUBMED_21437709}}~~	+

	==About this Structure==		==About this Structure==
Line 22:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:021437709</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021437709</ref><references group="xtra"/><references/>
	[[Category: Triticum aestivum]]		[[Category: Triticum aestivum]]
	[[Category: Bluethgen, N.]]		[[Category: Bluethgen, N.]]
Line 31:		Line 19:
	[[Category: Peroza, E A.]]		[[Category: Peroza, E A.]]
	[[Category: Zerbe, O.]]		[[Category: Zerbe, O.]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Metal-thiolate cluster]]
		+	[[Category: Metallothionein]]
		+	[[Category: Wheat ec-1]]
		+	[[Category: Zn binding]]

OCA at 05:36, 25 May 2011

OCA — Wed, 25 May 2011 05:36:52 GMT

←Older revision		Revision as of 05:36, 25 May 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:2l62.jpg\|left\|200px]]

-	The ~~entry 2l62~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2l62", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2l62\| PDB=2l62 \| SCENE= }}

-	~~Authors: Loebus, J., Peroza, E.A., Bluethgen, N., Fox, T., Meyer~~-~~Klaucke, W., Zerbe, O~~.~~, Freisinger, E~~.	+	===Protein and metal cluster structure of the wheat metallothionein domain g-Ec-1. The second part of the puzzle.===

-	~~Description~~: ~~Protein and metal cluster structure of~~ the ~~wheat metallothionein domain g~~-Ec-1. ~~The second part of the puzzle~~.	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21437709}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21437709 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21437709}}
		+
		+	==About this Structure==
		+	[[2l62]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L62 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021437709</ref><references group="xtra"/>
		+	[[Category: Triticum aestivum]]
		+	[[Category: Bluethgen, N.]]
		+	[[Category: Fox, T.]]
		+	[[Category: Freisinger, E.]]
		+	[[Category: Loebus, J.]]
		+	[[Category: Meyer-Klaucke, W.]]
		+	[[Category: Peroza, E A.]]
		+	[[Category: Zerbe, O.]]