2l8t - Revision history

OCA at 06:54, 1 May 2024

OCA — Wed, 01 May 2024 06:54:32 GMT

←Older revision		Revision as of 06:54, 1 May 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/O54465_STAAU O54465_STAAU]		[https://www.uniprot.org/uniprot/O54465_STAAU O54465_STAAU]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Staphylococcus aureus pathogenicity island 1 (SaPI1) is a mobile genetic element that carries genes for several superantigen toxins. SaPI1 is normally stably integrated into the host genome but can become mobilized by "helper" bacteriophage 80alpha, leading to the packaging of SaPI1 genomes into phage-like transducing particles that are composed of structural proteins supplied by the helper phage but having smaller capsids. We show that the SaPI1-encoded protein gp6 is necessary for efficient formation of small capsids. The NMR structure of gp6 reveals a dimeric protein with a helix-loop-helix motif similar to that of bacteriophage scaffolding proteins. The gp6 dimer matches internal densities that bridge capsid subunits in cryo-electron microscopy reconstructions of SaPI1 procapsids, suggesting that gp6 acts as an internal scaffolding protein in capsid size determination.
-
-	The Staphylococcus aureus Pathogenicity Island 1 Protein gp6 Functions as an Internal Scaffold during Capsid Size Determination.,Dearborn AD, Spilman MS, Damle PK, Chang JR, Monroe EB, Saad JS, Christie GE, Dokland T J Mol Biol. 2011 Sep 30;412(4):710-22. Epub 2011 Jul 29. PMID:21821042<ref>PMID:21821042</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2l8t" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:13, 15 November 2023

OCA — Wed, 15 Nov 2023 12:13:13 GMT

←Older revision		Revision as of 12:13, 15 November 2023
Line 1:		Line 1:

	==Staphylococcus aureus pathogenicity island 1 protein gp6, an internal scaffold in size determination==		==Staphylococcus aureus pathogenicity island 1 protein gp6, an internal scaffold in size determination==
-	<StructureSection load='2l8t' size='340' side='right'caption='[[2l8t~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2l8t' size='340' side='right'caption='[[2l8t]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L8T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L8T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [https://pdbe.org/2l8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [https://www.ebi.ac.uk/pdbsum/2l8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8t ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [https://pdbe.org/2l8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [https://www.ebi.ac.uk/pdbsum/2l8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8t ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O54465_STAAU O54465_STAAU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chang~~, J R~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: Christie~~, G E~~]]	+	[[Category: Chang JR]]
-	[[Category: Damle~~, P K~~]]	+	[[Category: Christie GE]]
-	[[Category: Dearborn~~, A D~~]]	+	[[Category: Damle PK]]
-	[[Category: Dokland, T]]	+	[[Category: Dearborn AD]]
-	[[Category: Monroe~~, E B~~]]	+	[[Category: Dokland T]]
-	[[Category: Saad~~, J S~~]]	+	[[Category: Monroe EB]]
-	[[Category: Spilman~~, M S]]~~	+	[[Category: Saad JS]]
-	~~[[Category: Bacteriophage]]~~	+	[[Category: Spilman MS]]
-	~~[[Category: Sapi]]~~	+
-	~~[[Category: Scaffold]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 07:49, 9 February 2022

OCA — Wed, 09 Feb 2022 07:49:31 GMT

←Older revision		Revision as of 07:49, 9 February 2022
Line 3:		Line 3:
	<StructureSection load='2l8t' size='340' side='right'caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2l8t' size='340' side='right'caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2L8T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L8T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [~~http~~://pdbe.org/2l8t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2l8t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8t ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [https://pdbe.org/2l8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [https://www.ebi.ac.uk/pdbsum/2l8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8t ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:00, 6 May 2020

OCA — Wed, 06 May 2020 07:00:42 GMT

←Older revision		Revision as of 07:00, 6 May 2020
Line 1:		Line 1:

	==Staphylococcus aureus pathogenicity island 1 protein gp6, an internal scaffold in size determination==		==Staphylococcus aureus pathogenicity island 1 protein gp6, an internal scaffold in size determination==
-	<StructureSection load='2l8t' size='340' side='right' caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2l8t' size='340' side='right'caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2L8T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2L8T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [http://pdbe.org/2l8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [http://www.ebi.ac.uk/pdbsum/2l8t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8t ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [http://pdbe.org/2l8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [http://www.ebi.ac.uk/pdbsum/2l8t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8t ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Chang, J R]]		[[Category: Chang, J R]]
	[[Category: Christie, G E]]		[[Category: Christie, G E]]

OCA at 19:50, 1 August 2018

OCA — Wed, 01 Aug 2018 19:50:43 GMT

←Older revision		Revision as of 19:50, 1 August 2018
Line 1:		Line 1:
		+
	==Staphylococcus aureus pathogenicity island 1 protein gp6, an internal scaffold in size determination==		==Staphylococcus aureus pathogenicity island 1 protein gp6, an internal scaffold in size determination==
	<StructureSection load='2l8t' size='340' side='right' caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2l8t' size='340' side='right' caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8T FirstGlance]. <br>		<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [http://pdbe.org/2l8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [http://www.ebi.ac.uk/pdbsum/2l8t PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [http://pdbe.org/2l8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [http://www.ebi.ac.uk/pdbsum/2l8t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8t ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:53, 10 September 2015

OCA — Thu, 10 Sep 2015 01:53:14 GMT

←Older revision		Revision as of 01:53, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2l8t' size='340' side='right' caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2l8t' size='340' side='right' caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus Staphylococcus~~ aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [http://www.ebi.ac.uk/pdbsum/2l8t PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [http://pdbe.org/2l8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [http://www.ebi.ac.uk/pdbsum/2l8t PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 13:		Line 13:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2l8t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Staphylococcus aureus]]
	[[Category: Chang, J R]]		[[Category: Chang, J R]]
	[[Category: Christie, G E]]		[[Category: Christie, G E]]

OCA at 06:32, 22 December 2014

OCA — Mon, 22 Dec 2014 06:32:56 GMT

←Older revision		Revision as of 06:32, 22 December 2014
Line 2:		Line 2:
	<StructureSection load='2l8t' size='340' side='right' caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2l8t' size='340' side='right' caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. ~~<br>~~	+	<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8T FirstGlance]. <br>
-	~~</td></tr><tr><td class="sblockLbl">~~<b>~~Activity:~~</b>~~</td><td class="sblockDat"><span class='plainlinks'>~~[http://en.~~wikipedia~~.~~org~~/~~wiki/Glucokinase Glucokinase], with EC number [http://www.brenda~~-~~enzymes.info~~/~~php~~/~~result_flat~~.~~php4~~?~~ecno~~=~~2.7.1.2 2.7.1.2~~] <~~/span~~></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [http://www.ebi.ac.uk/pdbsum/2l8t PDBsum]</span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [http://www.ebi.ac.uk/pdbsum/2l8t PDBsum]</span></td></tr>	+	</table>
-	<table>	+
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 12:		Line 11:
	The Staphylococcus aureus Pathogenicity Island 1 Protein gp6 Functions as an Internal Scaffold during Capsid Size Determination.,Dearborn AD, Spilman MS, Damle PK, Chang JR, Monroe EB, Saad JS, Christie GE, Dokland T J Mol Biol. 2011 Sep 30;412(4):710-22. Epub 2011 Jul 29. PMID:21821042<ref>PMID:21821042</ref>		The Staphylococcus aureus Pathogenicity Island 1 Protein gp6 Functions as an Internal Scaffold during Capsid Size Determination.,Dearborn AD, Spilman MS, Damle PK, Chang JR, Monroe EB, Saad JS, Christie GE, Dokland T J Mol Biol. 2011 Sep 30;412(4):710-22. Epub 2011 Jul 29. PMID:21821042<ref>PMID:21821042</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 19:		Line 18:
	</StructureSection>		</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Chang, J R.]]	+	[[Category: Chang, J R]]
-	[[Category: Christie, G E.]]	+	[[Category: Christie, G E]]
-	[[Category: Damle, P K.]]	+	[[Category: Damle, P K]]
-	[[Category: Dearborn, A D.]]	+	[[Category: Dearborn, A D]]
-	[[Category: Dokland, T.]]	+	[[Category: Dokland, T]]
-	[[Category: Monroe, E B.]]	+	[[Category: Monroe, E B]]
-	[[Category: Saad, J S.]]	+	[[Category: Saad, J S]]
-	[[Category: Spilman, M S.]]	+	[[Category: Spilman, M S]]
	[[Category: Bacteriophage]]		[[Category: Bacteriophage]]
	[[Category: Sapi]]		[[Category: Sapi]]
	[[Category: Scaffold]]		[[Category: Scaffold]]
	[[Category: Structural protein]]		[[Category: Structural protein]]

OCA at 09:44, 1 May 2014

OCA — Thu, 01 May 2014 09:44:48 GMT

←Older revision		Revision as of 09:44, 1 May 2014
Line 2:		Line 2:
	<StructureSection load='2l8t' size='340' side='right' caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2l8t' size='340' side='right' caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. <br>	+	<table><tr><td colspan='2'>[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. <br>
-	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>	+	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
-	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [http://www.ebi.ac.uk/pdbsum/2l8t PDBsum]</span><br>	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [http://www.ebi.ac.uk/pdbsum/2l8t PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	Staphylococcus aureus pathogenicity island 1 (SaPI1) is a mobile genetic element that carries genes for several superantigen toxins. SaPI1 is normally stably integrated into the host genome but can become mobilized by "helper" bacteriophage 80alpha, leading to the packaging of SaPI1 genomes into phage-like transducing particles that are composed of structural proteins supplied by the helper phage but having smaller capsids. We show that the SaPI1-encoded protein gp6 is necessary for efficient formation of small capsids. The NMR structure of gp6 reveals a dimeric protein with a helix-loop-helix motif similar to that of bacteriophage scaffolding proteins. The gp6 dimer matches internal densities that bridge capsid subunits in cryo-electron microscopy reconstructions of SaPI1 procapsids, suggesting that gp6 acts as an internal scaffolding protein in capsid size determination.		Staphylococcus aureus pathogenicity island 1 (SaPI1) is a mobile genetic element that carries genes for several superantigen toxins. SaPI1 is normally stably integrated into the host genome but can become mobilized by "helper" bacteriophage 80alpha, leading to the packaging of SaPI1 genomes into phage-like transducing particles that are composed of structural proteins supplied by the helper phage but having smaller capsids. We show that the SaPI1-encoded protein gp6 is necessary for efficient formation of small capsids. The NMR structure of gp6 reveals a dimeric protein with a helix-loop-helix motif similar to that of bacteriophage scaffolding proteins. The gp6 dimer matches internal densities that bridge capsid subunits in cryo-electron microscopy reconstructions of SaPI1 procapsids, suggesting that gp6 acts as an internal scaffolding protein in capsid size determination.
Line 11:		Line 13:

	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>		From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:06, 30 April 2014

OCA — Wed, 30 Apr 2014 10:06:16 GMT

←Older revision		Revision as of 10:06, 30 April 2014
Line 4:		Line 4:
	[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. <br>		[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. <br>
	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>		<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
		+	<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l8t RCSB], [http://www.ebi.ac.uk/pdbsum/2l8t PDBsum]</span><br>
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
	Staphylococcus aureus pathogenicity island 1 (SaPI1) is a mobile genetic element that carries genes for several superantigen toxins. SaPI1 is normally stably integrated into the host genome but can become mobilized by "helper" bacteriophage 80alpha, leading to the packaging of SaPI1 genomes into phage-like transducing particles that are composed of structural proteins supplied by the helper phage but having smaller capsids. We show that the SaPI1-encoded protein gp6 is necessary for efficient formation of small capsids. The NMR structure of gp6 reveals a dimeric protein with a helix-loop-helix motif similar to that of bacteriophage scaffolding proteins. The gp6 dimer matches internal densities that bridge capsid subunits in cryo-electron microscopy reconstructions of SaPI1 procapsids, suggesting that gp6 acts as an internal scaffolding protein in capsid size determination.		Staphylococcus aureus pathogenicity island 1 (SaPI1) is a mobile genetic element that carries genes for several superantigen toxins. SaPI1 is normally stably integrated into the host genome but can become mobilized by "helper" bacteriophage 80alpha, leading to the packaging of SaPI1 genomes into phage-like transducing particles that are composed of structural proteins supplied by the helper phage but having smaller capsids. We show that the SaPI1-encoded protein gp6 is necessary for efficient formation of small capsids. The NMR structure of gp6 reveals a dimeric protein with a helix-loop-helix motif similar to that of bacteriophage scaffolding proteins. The gp6 dimer matches internal densities that bridge capsid subunits in cryo-electron microscopy reconstructions of SaPI1 procapsids, suggesting that gp6 acts as an internal scaffolding protein in capsid size determination.

OCA at 08:28, 30 April 2014

OCA — Wed, 30 Apr 2014 08:28:13 GMT

←Older revision		Revision as of 08:28, 30 April 2014
Line 1:		Line 1:
-	[[~~Image:~~2l8t~~.png~~\|~~left\|200px~~]]	+	==Staphylococcus aureus pathogenicity island 1 protein gp6, an internal scaffold in size determination==
		+	<StructureSection load='2l8t' size='340' side='right' caption='[[2l8t]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	[[2l8t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA]. <br>
		+	<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
		+	== Publication Abstract from PubMed ==
		+	Staphylococcus aureus pathogenicity island 1 (SaPI1) is a mobile genetic element that carries genes for several superantigen toxins. SaPI1 is normally stably integrated into the host genome but can become mobilized by "helper" bacteriophage 80alpha, leading to the packaging of SaPI1 genomes into phage-like transducing particles that are composed of structural proteins supplied by the helper phage but having smaller capsids. We show that the SaPI1-encoded protein gp6 is necessary for efficient formation of small capsids. The NMR structure of gp6 reveals a dimeric protein with a helix-loop-helix motif similar to that of bacteriophage scaffolding proteins. The gp6 dimer matches internal densities that bridge capsid subunits in cryo-electron microscopy reconstructions of SaPI1 procapsids, suggesting that gp6 acts as an internal scaffolding protein in capsid size determination.

-	~~<!--~~	+	The Staphylococcus aureus Pathogenicity Island 1 Protein gp6 Functions as an Internal Scaffold during Capsid Size Determination.,Dearborn AD, Spilman MS, Damle PK, Chang JR, Monroe EB, Saad JS, Christie GE, Dokland T J Mol Biol. 2011 Sep 30;412(4):710-22. Epub 2011 Jul 29. PMID:21821042<ref>PMID:21821042</ref>
-	The ~~line below this paragraph~~, ~~containing "STRUCTURE_2l8t"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_2l8t\| PDB=2l8t \| SCENE= }}~~	+

-	~~===Staphylococcus aureus pathogenicity island 1 protein gp6~~, ~~an internal scaffold in size determination===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	== References ==
-		+	<references/>
-	~~<!--~~	+	__TOC__
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21821042}}, adds~~ the ~~Publication Abstract to the page~~	+	</StructureSection>
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21821042 is the PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_21821042}}~~	+
-		+
-	==~~About this Structure~~==	+
-	~~[[2l8t]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8T OCA].~~	+
-		+
-	~~==Reference==~~	+
-	<~~ref group="xtra">PMID:021821042</ref><references group="xtra"~~/>	+
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Chang, J R.]]		[[Category: Chang, J R.]]