2mel - Revision history

OCA at 11:55, 22 November 2023

OCA — Wed, 22 Nov 2023 11:55:32 GMT

←Older revision		Revision as of 11:55, 22 November 2023
Line 1:		Line 1:

	==NMR solution structure of the GS-TAMAPIN MUTATION R7A==		==NMR solution structure of the GS-TAMAPIN MUTATION R7A==
-	<StructureSection load='2mel' size='340' side='right'caption='[[2mel~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2mel' size='340' side='right'caption='[[2mel]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Buthus_tamalus Buthus tamalus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MEL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesobuthus_tamulus Mesobuthus tamulus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MEL FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[2me7\|2me7]], [[2men\|2men]], [[2meo\|2meo]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [https://pdbe.org/2mel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [https://www.ebi.ac.uk/pdbsum/2mel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mel ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [https://pdbe.org/2mel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [https://www.ebi.ac.uk/pdbsum/2mel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mel ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~KAX54_MESTA KAX54_MESTA]~~] Blocks small conductance calcium-activated potassium channels ~~in pyramidal neurons of the hippocampus~~. ~~Displays a unique, remarkable selectivity for SK2~~/KCNN2 ~~versus SK1~~/KCNN1 and ~~SK3~~/~~KCNN3 channels~~.<ref>PMID:12239213</ref>	+	[https://www.uniprot.org/uniprot/KAX54_HOTTA KAX54_HOTTA] Blocks small conductance calcium-activated potassium channels (PubMed:12239213). Shows activity on KCa2.2/KCNN2 (IC(50)=0.0243 nM), KCa2.3/KCNN3 (IC(50)=1.7 nM), and KCa2.1/KCNN1 (IC(50)=42 nM) (PubMed:12239213). Induces cell death when tested on Jurkat E6-1 and human mammary breast cancer MDA-MB-231 which constituvely express KCa2.2/KCNN2, but not on human peripheral blood lymphocytes (which do not express KCa2.2/KCNN2) (PubMed:24821061).<ref>PMID:12239213</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Buthus tamalus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ramirez-Cordero, B]]	+	[[Category: Mesobuthus tamulus]]
-	[[Category: Rio-Portilla, F ~~del]]~~	+	[[Category: Ramirez-Cordero B]]
-	~~[[Category: Alpha ktx5 4 mutant r7a]]~~	+	[[Category: Del Rio-Portilla F]]
-	~~[[Category: Scorpion toxin]]~~	+
-	~~[[Category: Tamapin]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 11:35, 16 February 2022

OCA — Wed, 16 Feb 2022 11:35:18 GMT

←Older revision		Revision as of 11:35, 16 February 2022
Line 3:		Line 3:
	<StructureSection load='2mel' size='340' side='right'caption='[[2mel]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mel' size='340' side='right'caption='[[2mel]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Buthus_tamalus Buthus tamalus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2MEL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Buthus_tamalus Buthus tamalus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MEL FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2me7\|2me7]], [[2men\|2men]], [[2meo\|2meo]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2me7\|2me7]], [[2men\|2men]], [[2meo\|2meo]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [~~http~~://pdbe.org/2mel PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2mel PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2mel ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [https://pdbe.org/2mel PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [https://www.ebi.ac.uk/pdbsum/2mel PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mel ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/KAX54_MESTA KAX54_MESTA]] Blocks small conductance calcium-activated potassium channels in pyramidal neurons of the hippocampus. Displays a unique, remarkable selectivity for SK2/KCNN2 versus SK1/KCNN1 and SK3/KCNN3 channels.<ref>PMID:12239213</ref>	+	[[https://www.uniprot.org/uniprot/KAX54_MESTA KAX54_MESTA]] Blocks small conductance calcium-activated potassium channels in pyramidal neurons of the hippocampus. Displays a unique, remarkable selectivity for SK2/KCNN2 versus SK1/KCNN1 and SK3/KCNN3 channels.<ref>PMID:12239213</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:22, 13 May 2020

OCA — Wed, 13 May 2020 06:22:59 GMT

←Older revision		Revision as of 06:22, 13 May 2020
Line 1:		Line 1:

	==NMR solution structure of the GS-TAMAPIN MUTATION R7A==		==NMR solution structure of the GS-TAMAPIN MUTATION R7A==
-	<StructureSection load='2mel' size='340' side='right' caption='[[2mel]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2mel' size='340' side='right'caption='[[2mel]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buthus_tamalus Buthus tamalus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2MEL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buthus_tamalus Buthus tamalus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2MEL FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2me7\|2me7]], [[2men\|2men]], [[2meo\|2meo]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2me7\|2me7]], [[2men\|2men]], [[2meo\|2meo]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [http://pdbe.org/2mel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [http://www.ebi.ac.uk/pdbsum/2mel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mel ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [http://pdbe.org/2mel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [http://www.ebi.ac.uk/pdbsum/2mel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mel ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Potassium channel toxin\|Potassium channel toxin]]	+	*[[Potassium channel toxin 3D structures\|Potassium channel toxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Buthus tamalus]]		[[Category: Buthus tamalus]]
		+	[[Category: Large Structures]]
	[[Category: Ramirez-Cordero, B]]		[[Category: Ramirez-Cordero, B]]
	[[Category: Rio-Portilla, F del]]		[[Category: Rio-Portilla, F del]]

OCA at 22:40, 9 August 2018

OCA — Thu, 09 Aug 2018 22:40:12 GMT

←Older revision		Revision as of 22:40, 9 August 2018
Line 1:		Line 1:
		+
	==NMR solution structure of the GS-TAMAPIN MUTATION R7A==		==NMR solution structure of the GS-TAMAPIN MUTATION R7A==
	<StructureSection load='2mel' size='340' side='right' caption='[[2mel]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mel' size='340' side='right' caption='[[2mel]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MEL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buthus_tamalus Buthus tamalus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MEL FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2me7\|2me7]], [[2men\|2men]], [[2meo\|2meo]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2me7\|2me7]], [[2men\|2men]], [[2meo\|2meo]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [http://pdbe.org/2mel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [http://www.ebi.ac.uk/pdbsum/2mel PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [http://pdbe.org/2mel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [http://www.ebi.ac.uk/pdbsum/2mel PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mel ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Buthus tamalus]]
	[[Category: Ramirez-Cordero, B]]		[[Category: Ramirez-Cordero, B]]
	[[Category: Rio-Portilla, F del]]		[[Category: Rio-Portilla, F del]]

OCA at 01:23, 12 September 2015

OCA — Sat, 12 Sep 2015 01:23:41 GMT

←Older revision		Revision as of 01:23, 12 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MEL FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MEL FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2me7\|2me7]], [[2men\|2men]], [[2meo\|2meo]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2me7\|2me7]], [[2men\|2men]], [[2meo\|2meo]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [http://www.ebi.ac.uk/pdbsum/2mel PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [http://pdbe.org/2mel PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [http://www.ebi.ac.uk/pdbsum/2mel PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2mel" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Potassium channel toxin\|Potassium channel toxin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:57, 25 December 2014

OCA — Thu, 25 Dec 2014 21:57:29 GMT

←Older revision		Revision as of 21:57, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MEL FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MEL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2me7\|2me7]], [[2men\|2men]], [[2meo\|2meo]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2me7\|2me7]], [[2men\|2men]], [[2meo\|2meo]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [http://www.ebi.ac.uk/pdbsum/2mel PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [http://www.ebi.ac.uk/pdbsum/2mel PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KAX54_MESTA KAX54_MESTA]] Blocks small conductance calcium-activated potassium channels in pyramidal neurons of the hippocampus. Displays a unique, remarkable selectivity for SK2/KCNN2 versus SK1/KCNN1 and SK3/KCNN3 channels.<ref>PMID:12239213</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Ramirez-Cordero, B.]]	+	[[Category: Ramirez-Cordero, B]]
-	[[Category: Rio-Portilla, F del.]]	+	[[Category: Rio-Portilla, F del]]
	[[Category: Alpha ktx5 4 mutant r7a]]		[[Category: Alpha ktx5 4 mutant r7a]]
	[[Category: Scorpion toxin]]		[[Category: Scorpion toxin]]
	[[Category: Tamapin]]		[[Category: Tamapin]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 06:32, 2 July 2014

OCA — Wed, 02 Jul 2014 06:32:41 GMT

←Older revision		Revision as of 06:32, 2 July 2014
Line 12:		Line 12:
	Cytotoxicity of Recombinant Tamapin and Related Toxin-Like Peptides on Model Cell Lines.,Ramirez-Cordero B, Toledano Y, Cano-Sanchez P, Hernandez-Lopez R, Flores-Solis D, Saucedo-Yanez AL, Chavez-Uribe I, Brieba LG, Del Rio-Portilla F Chem Res Toxicol. 2014 May 12. PMID:24821061<ref>PMID:24821061</ref>		Cytotoxicity of Recombinant Tamapin and Related Toxin-Like Peptides on Model Cell Lines.,Ramirez-Cordero B, Toledano Y, Cano-Sanchez P, Hernandez-Lopez R, Flores-Solis D, Saucedo-Yanez AL, Chavez-Uribe I, Brieba LG, Del Rio-Portilla F Chem Res Toxicol. 2014 May 12. PMID:24821061<ref>PMID:24821061</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 09:49, 28 May 2014

OCA — Wed, 28 May 2014 09:49:04 GMT

←Older revision		Revision as of 09:49, 28 May 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==NMR solution structure of the GS-TAMAPIN MUTATION R7A==
		+	<StructureSection load='2mel' size='340' side='right' caption='[[2mel]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2mel]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MEL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2me7\|2me7]], [[2men\|2men]], [[2meo\|2meo]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mel FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mel OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mel RCSB], [http://www.ebi.ac.uk/pdbsum/2mel PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The scorpion toxin tamapin displays the most potent and selective blockage against KCa2.2 channels known to date. In this work, we report the biosynthesis, three-dimensional structure, and cytotoxicity on cancer cell lines (Jurkat E6-1 and human mammary breast cancer MDA-MB-231) of recombinant tamapin and five related peptides bearing mutations on residues (R6A,R7A, R13A, R6A-R7A, and GS-tamapin) that were previously suggested to be important for tamapin's activity. The indicated cell lines were used as they constitutively express KCa2.2 channels. The studied toxin-like peptides displayed lethal responses on Jurkat T cells and breast cancer cells; their effect is dose- and time-dependent with IC50 values in the nanomolar range. The order of potency is r-tamapin > GS-tamapin > R6A > R13A > R6A-R7A > R7A for Jurkat T cells and r-tamapin > R7A for MDA-MB-231 breast cancer cells. Our structural determination by NMR demonstrated that r-tamapin preserves the folding of the alphaKTx5 subfamily and that neither single nor double alanine mutations affect the three-dimensional structure of the wild-type peptide. In contrast, our activity assays show that changes in cytotoxicity are related to the chemical nature of certain residues. Our results suggest that the toxic activity of r-tamapin on Jurkat and breast cancer cells could be mediated by the interaction of charged residues in tamapin with KCa2.2 channels via the apoptotic cell death pathway.

-	~~The entry 2mel is ON HOLD until Paper Publication~~	+	Cytotoxicity of Recombinant Tamapin and Related Toxin-Like Peptides on Model Cell Lines.,Ramirez-Cordero B, Toledano Y, Cano-Sanchez P, Hernandez-Lopez R, Flores-Solis D, Saucedo-Yanez AL, Chavez-Uribe I, Brieba LG, Del Rio-Portilla F Chem Res Toxicol. 2014 May 12. PMID:24821061<ref>PMID:24821061</ref>

-	~~Authors: del Rio-Portilla~~, F., Ramirez-Cordero, B.	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~NMR solution structure of the GS~~-~~TAMAPIN MUTATION R7A~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ramirez-Cordero, B.]]
		+	[[Category: Rio-Portilla, F del.]]
		+	[[Category: Alpha ktx5 4 mutant r7a]]
		+	[[Category: Scorpion toxin]]
		+	[[Category: Tamapin]]
		+	[[Category: Toxin]]

OCA at 05:20, 10 October 2013

OCA — Thu, 10 Oct 2013 05:20:34 GMT

←Older revision		Revision as of 05:20, 10 October 2013
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-	The entry 2mel is ON HOLD	+	The entry 2mel is ON HOLD until Paper Publication

	Authors: del Rio-Portilla, F., Ramirez-Cordero, B.		Authors: del Rio-Portilla, F., Ramirez-Cordero, B.

-	Description: NMR solution structure of the GS-TAMAPIN MUTATION R7A.	+	Description: NMR solution structure of the GS-TAMAPIN MUTATION R7A

OCA: Protected "2mel" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2013 06:25:14 GMT

Protected "2mel" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:25, 2 October 2013