2mi7 - Revision history

OCA at 06:05, 15 May 2024

OCA — Wed, 15 May 2024 06:05:50 GMT

←Older revision		Revision as of 06:05, 15 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [https://pdbe.org/2mi7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [https://www.ebi.ac.uk/pdbsum/2mi7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mi7 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [https://pdbe.org/2mi7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [https://www.ebi.ac.uk/pdbsum/2mi7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mi7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:36, 14 June 2023

OCA — Wed, 14 Jun 2023 09:36:09 GMT

←Older revision		Revision as of 09:36, 14 June 2023
Line 1:		Line 1:

	==Solution NMR structure of alpha3Y==		==Solution NMR structure of alpha3Y==
-	<StructureSection load='2mi7' size='340' side='right'caption='[[2mi7~~]], [[NMR_Ensembles_of_Models \| 32 NMR models~~]]' scene=''>	+	<StructureSection load='2mi7' size='340' side='right'caption='[[2mi7]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1lq7\|1lq7]], [[2lxy\|2lxy]]</div></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [https://pdbe.org/2mi7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [https://www.ebi.ac.uk/pdbsum/2mi7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mi7 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [https://pdbe.org/2mi7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [https://www.ebi.ac.uk/pdbsum/2mi7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mi7 ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Glover~~, S D~~]]	+	[[Category: Glover SD]]
-	[[Category: Hammarstrom, L]]	+	[[Category: Hammarstrom L]]
-	[[Category: Jorge, C]]	+	[[Category: Jorge C]]
-	[[Category: Liang, L]]	+	[[Category: Liang L]]
-	[[Category: Tommos, C]]	+	[[Category: Tommos C]]
-	[[Category: Valentine~~, K G]]~~	+	[[Category: Valentine KG]]
-	~~[[Category: De novo protein]]~~	+
-	~~[[Category: Proton-coupled electron transfer]]~~	+
-	~~[[Category: Redox protein]]~~	+
-	~~[[Category: Three-helix bundle]]~~	+
-	~~[[Category: Tyrosine radical~~]]	+

OCA at 11:36, 16 February 2022

OCA — Wed, 16 Feb 2022 11:36:09 GMT

←Older revision		Revision as of 11:36, 16 February 2022
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	<StructureSection load='2mi7' size='340' side='right'caption='[[2mi7]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>		<StructureSection load='2mi7' size='340' side='right'caption='[[2mi7]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq7\|1lq7]], [[2lxy\|2lxy]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1lq7\|1lq7]], [[2lxy\|2lxy]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [~~http~~://pdbe.org/2mi7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2mi7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2mi7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [https://pdbe.org/2mi7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [https://www.ebi.ac.uk/pdbsum/2mi7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mi7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:24, 13 May 2020

OCA — Wed, 13 May 2020 06:24:05 GMT

←Older revision		Revision as of 06:24, 13 May 2020
Line 1:		Line 1:

	==Solution NMR structure of alpha3Y==		==Solution NMR structure of alpha3Y==
-	<StructureSection load='2mi7' size='340' side='right' caption='[[2mi7]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>	+	<StructureSection load='2mi7' size='340' side='right'caption='[[2mi7]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq7\|1lq7]], [[2lxy\|2lxy]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq7\|1lq7]], [[2lxy\|2lxy]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [http://pdbe.org/2mi7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/2mi7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mi7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [http://pdbe.org/2mi7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/2mi7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mi7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Synthetic construct sequences]]		[[Category: Synthetic construct sequences]]
	[[Category: Glover, S D]]		[[Category: Glover, S D]]

OCA at 23:07, 9 August 2018

OCA — Thu, 09 Aug 2018 23:07:17 GMT

←Older revision		Revision as of 23:07, 9 August 2018
Line 1:		Line 1:
		+
	==Solution NMR structure of alpha3Y==		==Solution NMR structure of alpha3Y==
	<StructureSection load='2mi7' size='340' side='right' caption='[[2mi7]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>		<StructureSection load='2mi7' size='340' side='right' caption='[[2mi7]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq7\|1lq7]], [[2lxy\|2lxy]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq7\|1lq7]], [[2lxy\|2lxy]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [http://pdbe.org/2mi7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/2mi7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [http://pdbe.org/2mi7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/2mi7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mi7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Synthetic construct sequences]]
	[[Category: Glover, S D]]		[[Category: Glover, S D]]
	[[Category: Hammarstrom, L]]		[[Category: Hammarstrom, L]]

OCA at 13:16, 11 September 2015

OCA — Fri, 11 Sep 2015 13:16:26 GMT

←Older revision		Revision as of 13:16, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq7\|1lq7]], [[2lxy\|2lxy]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq7\|1lq7]], [[2lxy\|2lxy]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/2mi7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [http://pdbe.org/2mi7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/2mi7 PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2mi7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:51, 5 January 2015

OCA — Mon, 05 Jan 2015 14:51:54 GMT

←Older revision		Revision as of 14:51, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq7\|1lq7]], [[2lxy\|2lxy]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq7\|1lq7]], [[2lxy\|2lxy]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/2mi7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/2mi7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Glover, S D.]]	+	[[Category: Glover, S D]]
-	[[Category: Hammarstrom, L.]]	+	[[Category: Hammarstrom, L]]
-	[[Category: Jorge, C.]]	+	[[Category: Jorge, C]]
-	[[Category: Liang, L.]]	+	[[Category: Liang, L]]
-	[[Category: Tommos, C.]]	+	[[Category: Tommos, C]]
-	[[Category: Valentine, K G.]]	+	[[Category: Valentine, K G]]
	[[Category: De novo protein]]		[[Category: De novo protein]]
	[[Category: Proton-coupled electron transfer]]		[[Category: Proton-coupled electron transfer]]

OCA at 05:38, 27 August 2014

OCA — Wed, 27 Aug 2014 05:38:01 GMT

←Older revision		Revision as of 05:38, 27 August 2014
Line 8:		Line 8:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	~~Catalytically essential side~~-~~chain radicals have been recognized in~~ a ~~growing number of redox enzymes~~. ~~Here we present~~ a ~~novel approach to study this class of~~ redox ~~cofactors~~. ~~Our aim is to construct a de novo~~ protein, ~~a radical maquette, that will provide a protein framework in~~ which ~~to investigate how side-chain radicals are generated, controlled,~~ and ~~directed toward catalysis~~. ~~A tryptophan~~ and a ~~tyrosine radical maquette, denoted alpha~~(3)W(1) ~~and alpha~~(3)Y(1), ~~respectively, have been synthesized~~. ~~alpha~~(3)W(1) and ~~alpha~~(3)~~Y(1) contain 65 residues each~~ and ~~have molecular masses of 7~~.~~4 kDa~~. The ~~proteins differ only in residue 32, which~~ is the ~~position~~ of ~~their single aromatic side chain. Structural characterization reveals that the proteins fold in water solution into thermodynamically stable~~, ~~alpha~~-~~helical conformations with well~~-~~defined tertiary structures. The proteins are resistant to pH changes and remain stable through~~ the ~~physiological~~ pH ~~range~~. ~~The aromatic residues are shown to be located within the protein interior~~ and ~~shielded from the bulk phase~~, as ~~designed~~. ~~Differential pulse voltammetry was used~~ to ~~examine~~ the ~~reduction potentials~~ of the ~~aromatic side chains in alpha~~(3)W(1) ~~and alpha(3)Y(1) and compare them~~ to ~~the potentials of tryptophan and tyrosine when dissolved in water~~. ~~The tryptophan and tyrosine potentials were raised considerably when moved from a solution environment to a well-ordered protein milieu. We propose~~ that the ~~increase in reduction potential~~ of ~~the aromatic residues buried within~~ the protein~~, relative to the solution potentials, is due to a lack of~~ an ~~effective protonic contact between~~ the ~~aromatic residues~~ and ~~the bulk solution~~.	+	Tyrosine oxidation-reduction involves proton-coupled electron transfer (PCET) and a reactive radical state. These properties are effectively controlled in enzymes that use tyrosine as a high-potential, one-electron redox cofactor. The alpha3Y model protein contains Y32, which can be reversibly oxidized and reduced in voltammetry measurements. Structural and kinetic properties of alpha3Y are presented. A solution NMR structural analysis reveals that Y32 is the most deeply buried residue in alpha3Y. Time-resolved spectroscopy using a soluble flash-quench generated [Ru(2,2'-bipyridine)3]3+ oxidant provides high-quality Y32-O* absorption spectra. The rate constant of Y32 oxidation (kPCET) is pH dependent: 1.4 x 104 M-1 s-1 (pH 5.5), 1.8 x 105 M-1 s-1 (pH 8.5), 5.4 x 103 M-1 s-1 (pD 5.5), and 4.0 x 104 M-1 s-1 (pD 8.5). kH/kD of Y32 oxidation is 2.5 +/- 0.5 and 4.5 +/- 0.9 at pH(D) 5.5 and 8.5, respectively. These pH and isotope characteristics suggest a concerted or stepwise, proton-first Y32 oxidation mechanism. The photochemical yield of Y32-O* is 28-58% versus the concentration of [Ru(2,2'-bipyridine)3]3+. Y32-O* decays slowly, t1/2 in the range of 2-10 s, at both pH 5.5 and 8.5, via radical-radical dimerization as shown by second-order kinetics and fluorescence data. The high stability of Y32-O* is discussed relative to the structural properties of the Y32 site. Finally, the static alpha3Y NMR structure cannot explain (i) how the phenolic proton released upon oxidation is removed or (ii) how two Y32-O* come together to form dityrosine. These observations suggest that the dynamic properties of the protein ensemble may play an essential role in controlling the PCET and radical decay characteristics of alpha3Y.

-	~~De novo proteins as models of radical enzymes~~.,~~Tommos~~ C, ~~Skalicky JJ~~, ~~Pilloud DL~~, ~~Wand AJ~~, ~~Dutton PL Biochemistry~~. ~~1999 Jul 20;38(29):9495-507~~. PMID:~~10413527~~<ref>PMID:~~10413527~~</ref>	+	Photochemical Tyrosine Oxidation in the Structurally Well-Defined alphaY Protein: Proton-Coupled Electron Transfer and a Long-Lived Tyrosine Radical.,Glover SD, Jorge C, Liang L, Valentine KG, Hammarstrom L, Tommos C J Am Chem Soc. 2014 Aug 14. PMID:25121576<ref>PMID:25121576</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 16:15, 20 August 2014

OCA — Wed, 20 Aug 2014 16:15:36 GMT

←Older revision		Revision as of 16:15, 20 August 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Solution NMR structure of alpha3Y==
		+	<StructureSection load='2mi7' size='340' side='right' caption='[[2mi7]], [[NMR_Ensembles_of_Models \| 32 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2mi7]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MI7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1lq7\|1lq7]], [[2lxy\|2lxy]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mi7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mi7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mi7 RCSB], [http://www.ebi.ac.uk/pdbsum/2mi7 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Catalytically essential side-chain radicals have been recognized in a growing number of redox enzymes. Here we present a novel approach to study this class of redox cofactors. Our aim is to construct a de novo protein, a radical maquette, that will provide a protein framework in which to investigate how side-chain radicals are generated, controlled, and directed toward catalysis. A tryptophan and a tyrosine radical maquette, denoted alpha(3)W(1) and alpha(3)Y(1), respectively, have been synthesized. alpha(3)W(1) and alpha(3)Y(1) contain 65 residues each and have molecular masses of 7.4 kDa. The proteins differ only in residue 32, which is the position of their single aromatic side chain. Structural characterization reveals that the proteins fold in water solution into thermodynamically stable, alpha-helical conformations with well-defined tertiary structures. The proteins are resistant to pH changes and remain stable through the physiological pH range. The aromatic residues are shown to be located within the protein interior and shielded from the bulk phase, as designed. Differential pulse voltammetry was used to examine the reduction potentials of the aromatic side chains in alpha(3)W(1) and alpha(3)Y(1) and compare them to the potentials of tryptophan and tyrosine when dissolved in water. The tryptophan and tyrosine potentials were raised considerably when moved from a solution environment to a well-ordered protein milieu. We propose that the increase in reduction potential of the aromatic residues buried within the protein, relative to the solution potentials, is due to a lack of an effective protonic contact between the aromatic residues and the bulk solution.

-	~~The entry 2mi7 is ON HOLD until Paper Publication~~	+	De novo proteins as models of radical enzymes.,Tommos C, Skalicky JJ, Pilloud DL, Wand AJ, Dutton PL Biochemistry. 1999 Jul 20;38(29):9495-507. PMID:10413527<ref>PMID:10413527</ref>

-	~~Authors~~: Glover, S.D., Jorge, C., Liang, L., Valentine, K.G.~~, Hammarstrom, L., Tommos, C.~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Solution NMR structure of alpha3Y~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Glover, S D.]]
		+	[[Category: Hammarstrom, L.]]
		+	[[Category: Jorge, C.]]
		+	[[Category: Liang, L.]]
		+	[[Category: Tommos, C.]]
		+	[[Category: Valentine, K G.]]
		+	[[Category: De novo protein]]
		+	[[Category: Proton-coupled electron transfer]]
		+	[[Category: Redox protein]]
		+	[[Category: Three-helix bundle]]
		+	[[Category: Tyrosine radical]]

OCA: Protected "2mi7" [edit=sysop:move=sysop]

OCA — Wed, 18 Dec 2013 08:38:46 GMT

Protected "2mi7" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:38, 18 December 2013