2mjf - Revision history

OCA at 07:00, 1 May 2024

OCA — Wed, 01 May 2024 07:00:38 GMT

←Older revision		Revision as of 07:00, 1 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MJF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MJF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [https://pdbe.org/2mjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [https://www.ebi.ac.uk/pdbsum/2mjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjf ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [https://pdbe.org/2mjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [https://www.ebi.ac.uk/pdbsum/2mjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/RSA1_YEAST RSA1_YEAST] Involved in a late nucleoplasmic step of 60S ribosomal subunit assembly.		[https://www.uniprot.org/uniprot/RSA1_YEAST RSA1_YEAST] Involved in a late nucleoplasmic step of 60S ribosomal subunit assembly.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Biogenesis of eukaryotic box C/D small nucleolar ribonucleoprotein particles (C/D snoRNPs) involves conserved trans-acting factors, which are proposed to facilitate the assembly of the core proteins Snu13p/15.5K, Nop58p/NOP58, Nop56p/NOP56 and Nop1p/Fibrillarin on box C/D small nucleolar RNAs (C/D snoRNAs). In yeast, protein Rsa1 acts as a platform, interacting with both the RNA-binding core protein Snu13 and protein Pih1 of the Hsp82-R2TP chaperone complex. In this work, a proteomic approach coupled with functional and structural studies identifies protein Hit1 as a novel Rsa1p-interacting partner involved in C/D snoRNP assembly. Hit1p contributes to in vivo C/D snoRNA stability and pre-RNA maturation kinetics. It associates with U3 snoRNA precursors and influences its 3'-end processing. Remarkably, Hit1p is required to maintain steady-state levels of Rsa1p. This stabilizing activity is likely to be general across eukaryotic species, as the human protein ZNHIT3(TRIP3) showing sequence homology with Hit1p regulates the abundance of NUFIP1, the Rsa1p functional homolog. The nuclear magnetic resonance solution structure of the Rsa1p317-352-Hit1p70-164 complex reveals a novel mode of protein-protein association explaining the strong stability of the Rsa1p-Hit1p complex. Our biochemical data show that C/D snoRNAs and the core protein Nop58 can interact with the purified Snu13p-Rsa1p-Hit1p heterotrimer.
-
-	Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction.,Rothe B, Saliou JM, Quinternet M, Back R, Tiotiu D, Jacquemin C, Loegler C, Schlotter F, Pena V, Eckert K, Morera S, Van Dorsselaer A, Branlant C, Massenet S, Sanglier-Cianferani S, Manival X, Charpentier B Nucleic Acids Res. 2014 Aug 28. pii: gku612. PMID:25170085<ref>PMID:25170085</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2mjf" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:58, 8 March 2023

OCA — Wed, 08 Mar 2023 07:58:57 GMT

←Older revision		Revision as of 07:58, 8 March 2023
Line 1:		Line 1:

	==Solution structure of the complex between the yeast Rsa1 and Hit1 proteins==		==Solution structure of the complex between the yeast Rsa1 and Hit1 proteins==
-	<StructureSection load='2mjf' size='340' side='right'caption='[[2mjf~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2mjf' size='340' side='right'caption='[[2mjf]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MJF FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RSA1, YPL193W ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), HIT1, YJR055W, J1705 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [https://pdbe.org/2mjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [https://www.ebi.ac.uk/pdbsum/2mjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjf ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [https://pdbe.org/2mjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [https://www.ebi.ac.uk/pdbsum/2mjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjf ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RSA1_YEAST RSA1_YEAST]] Involved in a late nucleoplasmic step of 60S ribosomal subunit assembly.	+	[https://www.uniprot.org/uniprot/RSA1_YEAST RSA1_YEAST] Involved in a late nucleoplasmic step of 60S ribosomal subunit assembly.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Back, R]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Jacquemin, C]]	+	[[Category: Back R]]
-	[[Category: Manival, X]]	+	[[Category: Jacquemin C]]
-	[[Category: Quinternet, M]]	+	[[Category: Manival X]]
-	[[Category: Roth, B]]	+	[[Category: Quinternet M]]
-	~~[[Category: Protein binding~~]]	+	[[Category: Roth B]]

OCA at 15:15, 2 June 2021

OCA — Wed, 02 Jun 2021 15:15:44 GMT

←Older revision		Revision as of 15:15, 2 June 2021
Line 1:		Line 1:

	==Solution structure of the complex between the yeast Rsa1 and Hit1 proteins==		==Solution structure of the complex between the yeast Rsa1 and Hit1 proteins==
-	<StructureSection load='2mjf' size='340' side='right' caption='[[2mjf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2mjf' size='340' side='right'caption='[[2mjf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2MJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MJF FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RSA1, YPL193W ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), HIT1, YJR055W, J1705 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RSA1, YPL193W ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), HIT1, YJR055W, J1705 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [~~http~~://pdbe.org/2mjf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2mjf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [https://pdbe.org/2mjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [https://www.ebi.ac.uk/pdbsum/2mjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RSA1_YEAST RSA1_YEAST]] Involved in a late nucleoplasmic step of 60S ribosomal subunit assembly.	+	[[https://www.uniprot.org/uniprot/RSA1_YEAST RSA1_YEAST]] Involved in a late nucleoplasmic step of 60S ribosomal subunit assembly.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Back, R]]		[[Category: Back, R]]
	[[Category: Jacquemin, C]]		[[Category: Jacquemin, C]]

OCA at 22:30, 9 August 2018

OCA — Thu, 09 Aug 2018 22:30:03 GMT

←Older revision		Revision as of 22:30, 9 August 2018
Line 1:		Line 1:
		+
	==Solution structure of the complex between the yeast Rsa1 and Hit1 proteins==		==Solution structure of the complex between the yeast Rsa1 and Hit1 proteins==
	<StructureSection load='2mjf' size='340' side='right' caption='[[2mjf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mjf' size='340' side='right' caption='[[2mjf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MJF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [http://pdbe.org/2mjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [http://www.ebi.ac.uk/pdbsum/2mjf PDBsum]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RSA1, YPL193W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), HIT1, YJR055W, J1705 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [http://pdbe.org/2mjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [http://www.ebi.ac.uk/pdbsum/2mjf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
	[[Category: Back, R]]		[[Category: Back, R]]
	[[Category: Jacquemin, C]]		[[Category: Jacquemin, C]]

OCA at 11:08, 11 September 2015

OCA — Fri, 11 Sep 2015 11:08:39 GMT

←Older revision		Revision as of 11:08, 11 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MJF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MJF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [http://www.ebi.ac.uk/pdbsum/2mjf PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [http://pdbe.org/2mjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [http://www.ebi.ac.uk/pdbsum/2mjf PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2mjf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:38, 25 December 2014

OCA — Thu, 25 Dec 2014 15:38:17 GMT

←Older revision		Revision as of 15:38, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MJF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MJF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [http://www.ebi.ac.uk/pdbsum/2mjf PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [http://www.ebi.ac.uk/pdbsum/2mjf PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RSA1_YEAST RSA1_YEAST]] Involved in a late nucleoplasmic step of 60S ribosomal subunit assembly.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Back, R.]]	+	[[Category: Back, R]]
-	[[Category: Jacquemin, C.]]	+	[[Category: Jacquemin, C]]
-	[[Category: Manival, X.]]	+	[[Category: Manival, X]]
-	[[Category: Quinternet, M.]]	+	[[Category: Quinternet, M]]
-	[[Category: Roth, B.]]	+	[[Category: Roth, B]]
	[[Category: Protein binding]]		[[Category: Protein binding]]

OCA at 09:50, 10 September 2014

OCA — Wed, 10 Sep 2014 09:50:52 GMT

←Older revision		Revision as of 09:50, 10 September 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Solution structure of the complex between the yeast Rsa1 and Hit1 proteins==
		+	<StructureSection load='2mjf' size='340' side='right' caption='[[2mjf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MJF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [http://www.ebi.ac.uk/pdbsum/2mjf PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Biogenesis of eukaryotic box C/D small nucleolar ribonucleoprotein particles (C/D snoRNPs) involves conserved trans-acting factors, which are proposed to facilitate the assembly of the core proteins Snu13p/15.5K, Nop58p/NOP58, Nop56p/NOP56 and Nop1p/Fibrillarin on box C/D small nucleolar RNAs (C/D snoRNAs). In yeast, protein Rsa1 acts as a platform, interacting with both the RNA-binding core protein Snu13 and protein Pih1 of the Hsp82-R2TP chaperone complex. In this work, a proteomic approach coupled with functional and structural studies identifies protein Hit1 as a novel Rsa1p-interacting partner involved in C/D snoRNP assembly. Hit1p contributes to in vivo C/D snoRNA stability and pre-RNA maturation kinetics. It associates with U3 snoRNA precursors and influences its 3'-end processing. Remarkably, Hit1p is required to maintain steady-state levels of Rsa1p. This stabilizing activity is likely to be general across eukaryotic species, as the human protein ZNHIT3(TRIP3) showing sequence homology with Hit1p regulates the abundance of NUFIP1, the Rsa1p functional homolog. The nuclear magnetic resonance solution structure of the Rsa1p317-352-Hit1p70-164 complex reveals a novel mode of protein-protein association explaining the strong stability of the Rsa1p-Hit1p complex. Our biochemical data show that C/D snoRNAs and the core protein Nop58 can interact with the purified Snu13p-Rsa1p-Hit1p heterotrimer.

-	~~The entry 2mjf is ON HOLD until Paper Publication~~	+	Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction.,Rothe B, Saliou JM, Quinternet M, Back R, Tiotiu D, Jacquemin C, Loegler C, Schlotter F, Pena V, Eckert K, Morera S, Van Dorsselaer A, Branlant C, Massenet S, Sanglier-Cianferani S, Manival X, Charpentier B Nucleic Acids Res. 2014 Aug 28. pii: gku612. PMID:25170085<ref>PMID:25170085</ref>

-	~~Authors: Quinternet~~, M.~~, Roth, B~~., Back, R., Jacquemin, C., Manival, X.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Structure of Ribosome assembly 1 protein in complex with Hit1p~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Back, R.]]
		+	[[Category: Jacquemin, C.]]
		+	[[Category: Manival, X.]]
		+	[[Category: Quinternet, M.]]
		+	[[Category: Roth, B.]]
		+	[[Category: Protein binding]]

OCA at 06:35, 22 January 2014

OCA — Wed, 22 Jan 2014 06:35:07 GMT

←Older revision		Revision as of 06:35, 22 January 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 2mjf is ON HOLD	+	The entry 2mjf is ON HOLD until Paper Publication

	Authors: Quinternet, M., Roth, B., Back, R., Jacquemin, C., Manival, X.		Authors: Quinternet, M., Roth, B., Back, R., Jacquemin, C., Manival, X.

-	Description: ~~1H,13C and 15N assignment~~ of ~~Rsa1p(317-352)/~~Hit1p~~((70-164)~~	+	Description: Structure of Ribosome assembly 1 protein in complex with Hit1p

OCA: Protected "2mjf" [edit=sysop:move=sysop]

OCA — Wed, 15 Jan 2014 07:19:55 GMT

Protected "2mjf" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:19, 15 January 2014

OCA: New page: '''Unreleased structure''' The entry 2mjf is ON HOLD Authors: Quinternet, M., Roth, B., Back, R., Jacquemin, C., Manival, X. Description: 1H,13C and 15N assignment of Rsa1p(317-352)/Hi...

OCA — Wed, 15 Jan 2014 07:19:54 GMT

New page: '''Unreleased structure''' The entry 2mjf is ON HOLD Authors: Quinternet, M., Roth, B., Back, R., Jacquemin, C., Manival, X. Description: 1H,13C and 15N assignment of Rsa1p(317-352)/Hi...

New page

'''Unreleased structure'''

The entry 2mjf is ON HOLD

Authors: Quinternet, M., Roth, B., Back, R., Jacquemin, C., Manival, X.

Description: 1H,13C and 15N assignment of Rsa1p(317-352)/Hit1p((70-164)