2mlg - Revision history

OCA at 07:00, 1 May 2024

OCA — Wed, 01 May 2024 07:00:58 GMT

←Older revision		Revision as of 07:00, 1 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MLG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MLG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [https://pdbe.org/2mlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [https://www.ebi.ac.uk/pdbsum/2mlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlg ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [https://pdbe.org/2mlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [https://www.ebi.ac.uk/pdbsum/2mlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/O05472_SULIS O05472_SULIS]		[https://www.uniprot.org/uniprot/O05472_SULIS O05472_SULIS]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The hybrid plasmid-virus pSSVx from Sulfolobus islandicus presents an open reading frame encoding a 76 amino acid protein, namely Stf76, that does not show significant sequence homology with any protein with known 3D structure. The recombinant protein recognizes specifically two DNA-binding sites located in its own promoter, thus suggesting an auto-regulated role of its expression. Circular dichroism, spectrofluorimetric, light scattering and isothermal titration calorimetry experiments indicated a 2:1 molar ratio (protein:DNA) upon binding to the DNA target containing a single site. Furthermore, the solution structure of Stf76, determined by nuclear magnetic resonance (NMR) using chemical shift Rosetta software, has shown that the protein assumes a winged helix-turn-helix fold. NMR chemical shift perturbation analysis has been performed for the identification of the residues responsible for DNA interaction. In addition, a model of the Stf76-DNA complex has been built using as template a structurally related homolog.
-
-	Structural and functional studies of Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx: a novel peculiar member of the winged helix-turn-helix transcription factor family.,Contursi P, Farina B, Pirone L, Fusco S, Russo L, Bartolucci S, Fattorusso R, Pedone E Nucleic Acids Res. 2014 May 1;42(9):5993-6011. doi: 10.1093/nar/gku215. Epub 2014, Mar 25. PMID:24682827<ref>PMID:24682827</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2mlg" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:00, 8 March 2023

OCA — Wed, 08 Mar 2023 08:00:52 GMT

←Older revision		Revision as of 08:00, 8 March 2023
Line 1:		Line 1:

	==Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx==		==Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx==
-	<StructureSection load='2mlg' size='340' side='right'caption='[[2mlg~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2mlg' size='340' side='right'caption='[[2mlg]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MLG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MLG FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [https://pdbe.org/2mlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [https://www.ebi.ac.uk/pdbsum/2mlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlg ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [https://pdbe.org/2mlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [https://www.ebi.ac.uk/pdbsum/2mlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O05472_SULIS O05472_SULIS]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	[[Category: Fuselloviridae]]		[[Category: Fuselloviridae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Farina, B]]	+	[[Category: Farina B]]
-	[[Category: Fattorusso, R]]	+	[[Category: Fattorusso R]]
-	[[Category: Russo, L]]	+	[[Category: Russo L]]
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Winged helix domain~~]]	+

OCA at 15:16, 2 June 2021

OCA — Wed, 02 Jun 2021 15:16:51 GMT

←Older revision		Revision as of 15:16, 2 June 2021
Line 3:		Line 3:
	<StructureSection load='2mlg' size='340' side='right'caption='[[2mlg]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mlg' size='340' side='right'caption='[[2mlg]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2MLG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MLG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [~~http~~://pdbe.org/2mlg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2mlg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlg ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [https://pdbe.org/2mlg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [https://www.ebi.ac.uk/pdbsum/2mlg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:04, 21 August 2019

OCA — Wed, 21 Aug 2019 07:04:38 GMT

←Older revision		Revision as of 07:04, 21 August 2019
Line 1:		Line 1:

	==Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx==		==Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx==
-	<StructureSection load='2mlg' size='340' side='right' caption='[[2mlg]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2mlg' size='340' side='right'caption='[[2mlg]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MLG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MLG FirstGlance]. <br>
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Fuselloviridae]]		[[Category: Fuselloviridae]]
		+	[[Category: Large Structures]]
	[[Category: Farina, B]]		[[Category: Farina, B]]
	[[Category: Fattorusso, R]]		[[Category: Fattorusso, R]]

OCA at 14:00, 15 November 2017

OCA — Wed, 15 Nov 2017 14:00:16 GMT

←Older revision		Revision as of 14:00, 15 November 2017
Line 1:		Line 1:
		+
	==Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx==		==Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx==
	<StructureSection load='2mlg' size='340' side='right' caption='[[2mlg]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mlg' size='340' side='right' caption='[[2mlg]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MLG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MLG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [http://pdbe.org/2mlg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [http://www.ebi.ac.uk/pdbsum/2mlg PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [http://pdbe.org/2mlg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [http://www.ebi.ac.uk/pdbsum/2mlg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:56, 10 September 2015

OCA — Thu, 10 Sep 2015 23:56:01 GMT

←Older revision		Revision as of 23:56, 10 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MLG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MLG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [http://www.ebi.ac.uk/pdbsum/2mlg PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [http://pdbe.org/2mlg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [http://www.ebi.ac.uk/pdbsum/2mlg PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 13:		Line 13:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2mlg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:09, 3 January 2015

OCA — Sat, 03 Jan 2015 21:09:40 GMT

←Older revision		Revision as of 21:09, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MLG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MLG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [http://www.ebi.ac.uk/pdbsum/2mlg PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [http://www.ebi.ac.uk/pdbsum/2mlg PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 11:		Line 11:
	Structural and functional studies of Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx: a novel peculiar member of the winged helix-turn-helix transcription factor family.,Contursi P, Farina B, Pirone L, Fusco S, Russo L, Bartolucci S, Fattorusso R, Pedone E Nucleic Acids Res. 2014 May 1;42(9):5993-6011. doi: 10.1093/nar/gku215. Epub 2014, Mar 25. PMID:24682827<ref>PMID:24682827</ref>		Structural and functional studies of Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx: a novel peculiar member of the winged helix-turn-helix transcription factor family.,Contursi P, Farina B, Pirone L, Fusco S, Russo L, Bartolucci S, Fattorusso R, Pedone E Nucleic Acids Res. 2014 May 1;42(9):5993-6011. doi: 10.1093/nar/gku215. Epub 2014, Mar 25. PMID:24682827<ref>PMID:24682827</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 18:		Line 18:
	</StructureSection>		</StructureSection>
	[[Category: Fuselloviridae]]		[[Category: Fuselloviridae]]
-	[[Category: Farina, B.]]	+	[[Category: Farina, B]]
-	[[Category: Fattorusso, R.]]	+	[[Category: Fattorusso, R]]
-	[[Category: Russo, L.]]	+	[[Category: Russo, L]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Winged helix domain]]		[[Category: Winged helix domain]]

OCA at 04:38, 4 June 2014

OCA — Wed, 04 Jun 2014 04:38:52 GMT

←Older revision		Revision as of 04:38, 4 June 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2mlg\| PDB=2mlg \| SCENE= }}~~	+	==Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx==
-	===Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx===	+	<StructureSection load='2mlg' size='340' side='right' caption='[[2mlg]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2mlg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fuselloviridae Fuselloviridae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MLG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mlg RCSB], [http://www.ebi.ac.uk/pdbsum/2mlg PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The hybrid plasmid-virus pSSVx from Sulfolobus islandicus presents an open reading frame encoding a 76 amino acid protein, namely Stf76, that does not show significant sequence homology with any protein with known 3D structure. The recombinant protein recognizes specifically two DNA-binding sites located in its own promoter, thus suggesting an auto-regulated role of its expression. Circular dichroism, spectrofluorimetric, light scattering and isothermal titration calorimetry experiments indicated a 2:1 molar ratio (protein:DNA) upon binding to the DNA target containing a single site. Furthermore, the solution structure of Stf76, determined by nuclear magnetic resonance (NMR) using chemical shift Rosetta software, has shown that the protein assumes a winged helix-turn-helix fold. NMR chemical shift perturbation analysis has been performed for the identification of the residues responsible for DNA interaction. In addition, a model of the Stf76-DNA complex has been built using as template a structurally related homolog.

-	~~==About this Structure==~~	+	Structural and functional studies of Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx: a novel peculiar member of the winged helix-turn-helix transcription factor family.,Contursi P, Farina B, Pirone L, Fusco S, Russo L, Bartolucci S, Fattorusso R, Pedone E Nucleic Acids Res. 2014 May 1;42(9):5993-6011. doi: 10.1093/nar/gku215. Epub 2014, Mar 25. PMID:24682827<ref>PMID:24682827</ref>
-	~~[[2mlg]] is~~ a 1 ~~chain structure~~. ~~Full experimental information is available from [http~~://~~oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin~~/~~ocashort?id~~=~~2MLG OCA~~].	+
		+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fuselloviridae]]
	[[Category: Farina, B.]]		[[Category: Farina, B.]]
	[[Category: Fattorusso, R.]]		[[Category: Fattorusso, R.]]

OCA: Protected "2mlg" [edit=sysop:move=sysop]

OCA — Wed, 12 Mar 2014 13:53:24 GMT

Protected "2mlg" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:53, 12 March 2014

OCA: New page: {{STRUCTURE_2mlg| PDB=2mlg | SCENE= }} ===Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx=== ==About this Structure== 2mlg is a 1 chain structure. Full experimental info...

OCA — Wed, 12 Mar 2014 13:53:22 GMT

New page: {{STRUCTURE_2mlg| PDB=2mlg | SCENE= }} ===Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx=== ==About this Structure== 2mlg is a 1 chain structure. Full experimental info...

New page

{{STRUCTURE_2mlg| PDB=2mlg | SCENE= }}
===Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx===

==About this Structure==
[[2mlg]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLG OCA].
[[Category: Farina, B.]]
[[Category: Fattorusso, R.]]
[[Category: Russo, L.]]
[[Category: Dna binding protein]]
[[Category: Winged helix domain]]