2mqf - Revision history

OCA at 09:42, 14 June 2023

OCA — Wed, 14 Jun 2023 09:42:58 GMT

←Older revision		Revision as of 09:42, 14 June 2023
Line 1:		Line 1:

	==NMR structure of spider toxin-TRTX-Hhn2b==		==NMR structure of spider toxin-TRTX-Hhn2b==
-	<StructureSection load='2mqf' size='340' side='right'caption='[[2mqf~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2mqf' size='340' side='right'caption='[[2mqf]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mqf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chinese_bird_spider Chinese bird spider~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MQF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mqf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haplopelma_hainanum Haplopelma hainanum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MQF FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqf OCA], [https://pdbe.org/2mqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mqf RCSB], [https://www.ebi.ac.uk/pdbsum/2mqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mqf ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqf OCA], [https://pdbe.org/2mqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mqf RCSB], [https://www.ebi.ac.uk/pdbsum/2mqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mqf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~H1A03_HAPHA H1A03_HAPHA~~]~~] Is a depressant toxin. Blocks~~ the SCN2A/SCN1B (Nav1.2/~~Beta1~~) sodium channel ~~receptor~~ (IC(50) is 68 ~~+-6~~ uM) and the insect sodium channel para/tipE (IC(50) is 4~~.3 +-0~~.3 uM), without altering the activation or inactivation kinetics.<ref>PMID:14675784</ref>	+	[https://www.uniprot.org/uniprot/H1A03_CYRHA H1A03_CYRHA] Weakly blocks the rat SCN2A/SCN1B (Nav1.2/beta-1) sodium channel (IC(50)=68 uM) and the insect sodium channel para/tipE (IC(50)=4.3 uM), without altering the activation or inactivation kinetics (depressant toxin).<ref>PMID:14675784</ref> <ref>PMID:26429937</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chinese bird spider~~]]	+	[[Category: Haplopelma hainanum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chin~~, Y K.Y~~]]	+	[[Category: Chin YKY]]
-	[[Category: Klint~~, J K~~]]	+	[[Category: Klint JK]]
-	[[Category: Mobli, M]]	+	[[Category: Mobli M]]
-	~~[[Category: Nav1 7]]~~	+
-	~~[[Category: Spider toxin]]~~	+
-	~~[[Category: Toxin]]~~	+
-	~~[[Category: Voltage gated ion channel~~]]	+

OCA at 09:02, 23 February 2022

OCA — Wed, 23 Feb 2022 09:02:32 GMT

←Older revision		Revision as of 09:02, 23 February 2022
Line 3:		Line 3:
	<StructureSection load='2mqf' size='340' side='right'caption='[[2mqf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mqf' size='340' side='right'caption='[[2mqf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mqf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chinese_bird_spider Chinese bird spider]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2MQF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mqf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chinese_bird_spider Chinese bird spider]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MQF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2mqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqf OCA], [~~http~~://pdbe.org/2mqf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2mqf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2mqf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2mqf ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqf OCA], [https://pdbe.org/2mqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mqf RCSB], [https://www.ebi.ac.uk/pdbsum/2mqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mqf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/H1A03_HAPHA H1A03_HAPHA]] Is a depressant toxin. Blocks the SCN2A/SCN1B (Nav1.2/Beta1) sodium channel receptor (IC(50) is 68 +-6 uM) and the insect sodium channel para/tipE (IC(50) is 4.3 +-0.3 uM), without altering the activation or inactivation kinetics.<ref>PMID:14675784</ref>	+	[[https://www.uniprot.org/uniprot/H1A03_HAPHA H1A03_HAPHA]] Is a depressant toxin. Blocks the SCN2A/SCN1B (Nav1.2/Beta1) sodium channel receptor (IC(50) is 68 +-6 uM) and the insect sodium channel para/tipE (IC(50) is 4.3 +-0.3 uM), without altering the activation or inactivation kinetics.<ref>PMID:14675784</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:27, 20 May 2020

OCA — Wed, 20 May 2020 06:27:16 GMT

←Older revision		Revision as of 06:27, 20 May 2020
Line 1:		Line 1:

	==NMR structure of spider toxin-TRTX-Hhn2b==		==NMR structure of spider toxin-TRTX-Hhn2b==
-	<StructureSection load='2mqf' size='340' side='right' caption='[[2mqf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2mqf' size='340' side='right'caption='[[2mqf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chinese_bird_spider Chinese bird spider]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQF OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2MQF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chinese_bird_spider Chinese bird spider]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2MQF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2mqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqf OCA], [http://pdbe.org/2mqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mqf RCSB], [http://www.ebi.ac.uk/pdbsum/2mqf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mqf ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2mqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqf OCA], [http://pdbe.org/2mqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mqf RCSB], [http://www.ebi.ac.uk/pdbsum/2mqf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mqf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Chinese bird spider]]		[[Category: Chinese bird spider]]
		+	[[Category: Large Structures]]
	[[Category: Chin, Y K.Y]]		[[Category: Chin, Y K.Y]]
	[[Category: Klint, J K]]		[[Category: Klint, J K]]

OCA at 17:18, 15 August 2018

OCA — Wed, 15 Aug 2018 17:18:57 GMT

←Older revision		Revision as of 17:18, 15 August 2018
Line 1:		Line 1:
		+
	==NMR structure of spider toxin-TRTX-Hhn2b==		==NMR structure of spider toxin-TRTX-Hhn2b==
	<StructureSection load='2mqf' size='340' side='right' caption='[[2mqf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2mqf' size='340' side='right' caption='[[2mqf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2mqf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MQF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2mqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chinese_bird_spider Chinese bird spider]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MQF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqf OCA], [http://pdbe.org/2mqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mqf RCSB], [http://www.ebi.ac.uk/pdbsum/2mqf PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqf OCA], [http://pdbe.org/2mqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mqf RCSB], [http://www.ebi.ac.uk/pdbsum/2mqf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mqf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chinese bird spider]]
	[[Category: Chin, Y K.Y]]		[[Category: Chin, Y K.Y]]
	[[Category: Klint, J K]]		[[Category: Klint, J K]]

OCA at 06:26, 23 December 2015

OCA — Wed, 23 Dec 2015 06:26:37 GMT

←Older revision		Revision as of 06:26, 23 December 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2mqf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MQF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2mqf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MQF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mqf RCSB], [http://www.ebi.ac.uk/pdbsum/2mqf PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqf OCA], [http://pdbe.org/2mqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mqf RCSB], [http://www.ebi.ac.uk/pdbsum/2mqf PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/H1A03_HAPHA H1A03_HAPHA]] Is a depressant toxin. Blocks the SCN2A/SCN1B (Nav1.2/Beta1) sodium channel receptor (IC(50) is 68 +-6 uM) and the insect sodium channel para/tipE (IC(50) is 4.3 +-0.3 uM), without altering the activation or inactivation kinetics.<ref>PMID:14675784</ref>		[[http://www.uniprot.org/uniprot/H1A03_HAPHA H1A03_HAPHA]] Is a depressant toxin. Blocks the SCN2A/SCN1B (Nav1.2/Beta1) sodium channel receptor (IC(50) is 68 +-6 uM) and the insect sodium channel para/tipE (IC(50) is 4.3 +-0.3 uM), without altering the activation or inactivation kinetics.<ref>PMID:14675784</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many spider-venom peptides are known to modulate the activity of the voltage-gated sodium (NaV) subtype 1.7 (NaV1.7) channel, which has emerged as a promising analgesic target. In particular, a class of spider-venom peptides (NaSpTx1) has been found to potently inhibit NaV1.7 (nanomolar IC50), and has been shown to produce analgesic effects in animals. However, one member of this family [micro-TRTX-Hhn2b (Hhn2b)] does not inhibit mammalian NaV channels expressed in dorsal root ganglia at concentrations up to 100 microM. This peptide is classified as a NaSpTx1 member by virtue of its cysteine spacing and sequence conservation over functionally important residues. Here, we have performed detailed structural and functional analyses of Hhn2b, leading us to identify two nonpharmacophore residues that contribute to human NaV1.7 (hNaV1.7) inhibition by nonoverlapping mechanisms. These findings allowed us to produce a double mutant of Hhn2b that shows nanomolar inhibition of hNaV1.7. Traditional structure/function analysis did not provide sufficient resolution to identify the mechanism underlying the observed gain of function. However, by solving the high-resolution structure of both the wild-type and mutant peptides using advanced multidimensional NMR experiments, we were able to uncover a previously unknown network of interactions that stabilize the pharmacophore region of this class of venom peptides. We further monitored the lipid binding properties of the peptides and identified that one of the key amino acid substitutions also selectively modulates the binding of the peptide to anionic lipids. These results will further aid the development of peptide-based analgesics for the treatment of chronic pain.
		+
		+	Rational Engineering Defines a Molecular Switch That Is Essential for Activity of Spider-Venom Peptides against the Analgesics Target NaV1.7.,Klint JK, Chin YK, Mobli M Mol Pharmacol. 2015 Dec;88(6):1002-10. doi: 10.1124/mol.115.100784. Epub 2015 Oct, 1. PMID:26429937<ref>PMID:26429937</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 2mqf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:28, 15 July 2015

OCA — Wed, 15 Jul 2015 13:28:48 GMT

←Older revision		Revision as of 13:28, 15 July 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==NMR structure of spider toxin-TRTX-Hhn2b==
-		+	<StructureSection load='2mqf' size='340' side='right' caption='[[2mqf]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~The entry~~ 2mqf is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[2mqf]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MQF FirstGlance]. <br>
-	~~Authors~~: ~~Klint, J~~.K., ~~Chin~~, Y.K.Y., ~~Mobli, M~~.	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mqf RCSB], [http://www.ebi.ac.uk/pdbsum/2mqf PDBsum]</span></td></tr>
-		+	</table>
-	~~Description~~: ~~NMR structure of spider~~ toxin-~~TRTX~~-~~Hhn2b~~	+	== Function ==
-	[[Category: ~~Unreleased Structures~~]]	+	[[http://www.uniprot.org/uniprot/H1A03_HAPHA H1A03_HAPHA]] Is a depressant toxin. Blocks the SCN2A/SCN1B (Nav1.2/Beta1) sodium channel receptor (IC(50) is 68 +-6 uM) and the insect sodium channel para/tipE (IC(50) is 4.3 +-0.3 uM), without altering the activation or inactivation kinetics.<ref>PMID:14675784</ref>
-	[[Category: ~~Chin~~, Y.K.Y]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chin, Y K.Y]]
		+	[[Category: Klint, J K]]
	[[Category: Mobli, M]]		[[Category: Mobli, M]]
-	[[Category: ~~Klint, J.K~~]]	+	[[Category: Nav1 7]]
		+	[[Category: Spider toxin]]
		+	[[Category: Toxin]]
		+	[[Category: Voltage gated ion channel]]

OCA at 12:16, 24 December 2014

OCA — Wed, 24 Dec 2014 12:16:08 GMT

←Older revision		Revision as of 12:16, 24 December 2014
Line 6:		Line 6:

	Description: NMR structure of spider toxin-TRTX-Hhn2b		Description: NMR structure of spider toxin-TRTX-Hhn2b
		+	[[Category: Unreleased Structures]]
		+	[[Category: Chin, Y.K.Y]]
		+	[[Category: Mobli, M]]
		+	[[Category: Klint, J.K]]

OCA at 06:44, 30 July 2014

OCA — Wed, 30 Jul 2014 06:44:39 GMT

←Older revision		Revision as of 06:44, 30 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 2mqf is ON HOLD	+	The entry 2mqf is ON HOLD until Paper Publication

	Authors: Klint, J.K., Chin, Y.K.Y., Mobli, M.		Authors: Klint, J.K., Chin, Y.K.Y., Mobli, M.

	Description: NMR structure of spider toxin-TRTX-Hhn2b		Description: NMR structure of spider toxin-TRTX-Hhn2b

OCA at 09:25, 16 July 2014

OCA — Wed, 16 Jul 2014 09:25:44 GMT

←Older revision		Revision as of 09:25, 16 July 2014
Line 5:		Line 5:
	Authors: Klint, J.K., Chin, Y.K.Y., Mobli, M.		Authors: Klint, J.K., Chin, Y.K.Y., Mobli, M.

-	Description: NMR structure of spider toxin -TRTX-Hhn2b	+	Description: NMR structure of spider toxin-TRTX-Hhn2b

OCA: Protected "2mqf" [edit=sysop:move=sysop]

OCA — Wed, 25 Jun 2014 05:30:27 GMT

Protected "2mqf" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:30, 25 June 2014