2n6b - Revision history

OCA at 18:57, 29 November 2023

OCA — Wed, 29 Nov 2023 18:57:44 GMT

←Older revision		Revision as of 18:57, 29 November 2023
Line 1:		Line 1:

	==NMR structure of the de-novo toxin Hui1==		==NMR structure of the de-novo toxin Hui1==
-	<StructureSection load='2n6b' size='340' side='right'caption='[[2n6b~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2n6b' size='340' side='right'caption='[[2n6b]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n6b]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N6B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n6b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N6B FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6b OCA], [https://pdbe.org/2n6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n6b RCSB], [https://www.ebi.ac.uk/pdbsum/2n6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6b ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6b OCA], [https://pdbe.org/2n6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n6b RCSB], [https://www.ebi.ac.uk/pdbsum/2n6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6b ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chill, J H~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Goldstein, S A.N~~]]	+	[[Category: Chill JH]]
-	[[Category: ~~Mendelman, N~~]]	+	[[Category: Goldstein SAN]]
-	[[Category: ~~Zhao, R~~]]	+	[[Category: Mendelman N]]
-	[[Category: ~~Potassium channel]]~~	+	[[Category: Zhao R]]
-	~~[[Category: Shk]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 09:05, 23 February 2022

OCA — Wed, 23 Feb 2022 09:05:19 GMT

←Older revision		Revision as of 09:05, 23 February 2022
Line 3:		Line 3:
	<StructureSection load='2n6b' size='340' side='right'caption='[[2n6b]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2n6b' size='340' side='right'caption='[[2n6b]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n6b]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6B OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2N6B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n6b]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N6B FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2n6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6b OCA], [~~http~~://pdbe.org/2n6b PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2n6b RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2n6b PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6b ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6b OCA], [https://pdbe.org/2n6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n6b RCSB], [https://www.ebi.ac.uk/pdbsum/2n6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6b ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:31, 20 May 2020

OCA — Wed, 20 May 2020 06:31:20 GMT

←Older revision		Revision as of 06:31, 20 May 2020
Line 1:		Line 1:

	==NMR structure of the de-novo toxin Hui1==		==NMR structure of the de-novo toxin Hui1==
-	<StructureSection load='2n6b' size='340' side='right' caption='[[2n6b]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2n6b' size='340' side='right'caption='[[2n6b]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n6b]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6B OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2N6B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n6b]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6B OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2N6B FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2n6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6b OCA], [http://pdbe.org/2n6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n6b RCSB], [http://www.ebi.ac.uk/pdbsum/2n6b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6b ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2n6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6b OCA], [http://pdbe.org/2n6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n6b RCSB], [http://www.ebi.ac.uk/pdbsum/2n6b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6b ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Chill, J H]]		[[Category: Chill, J H]]
	[[Category: Goldstein, S A.N]]		[[Category: Goldstein, S A.N]]

OCA at 17:10, 15 August 2018

OCA — Wed, 15 Aug 2018 17:10:07 GMT

←Older revision		Revision as of 17:10, 15 August 2018
Line 1:		Line 1:
		+
	==NMR structure of the de-novo toxin Hui1==		==NMR structure of the de-novo toxin Hui1==
	<StructureSection load='2n6b' size='340' side='right' caption='[[2n6b]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2n6b' size='340' side='right' caption='[[2n6b]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2n6b]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N6B FirstGlance]. <br>		<table><tr><td colspan='2'>[[2n6b]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N6B FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6b OCA], [http://pdbe.org/2n6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n6b RCSB], [http://www.ebi.ac.uk/pdbsum/2n6b PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6b OCA], [http://pdbe.org/2n6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n6b RCSB], [http://www.ebi.ac.uk/pdbsum/2n6b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6b ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:50, 16 December 2015

OCA — Wed, 16 Dec 2015 13:50:54 GMT

←Older revision		Revision as of 13:50, 16 December 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==NMR structure of the de-novo toxin Hui1==
		+	<StructureSection load='2n6b' size='340' side='right' caption='[[2n6b]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2n6b]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N6B FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6b OCA], [http://pdbe.org/2n6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n6b RCSB], [http://www.ebi.ac.uk/pdbsum/2n6b PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Peptide neurotoxins are powerful tools for research, diagnosis, and treatment of disease. Limiting broader use, most receptors lack an identified toxin that binds with high affinity and specificity. This paper describes isolation of toxins for one such orphan target, KcsA, a potassium channel that has been fundamental to delineating the structural basis for ion channel function. A phage-display strategy is presented whereby approximately 1.5 million novel and natural peptides are fabricated on the scaffold present in ShK, a sea anemone type I (SAK1) toxin stabilized by three disulfide bonds. We describe two toxins selected by sorting on purified KcsA, one novel (Hui1, 34 residues) and one natural (HmK, 35 residues). Hui1 is potent, blocking single KcsA channels in planar lipid bilayers half-maximally (Ki) at 1 nM. Hui1 is also specific, inhibiting KcsA-Shaker channels in Xenopus oocytes with a Ki of 0.5 nM whereas Shaker, Kv1.2, and Kv1.3 channels are blocked over 200-fold less well. HmK is potent but promiscuous, blocking KcsA-Shaker, Shaker, Kv1.2, and Kv1.3 channels with Ki of 1-4 nM. As anticipated, one Hui1 blocks the KcsA pore and two conserved toxin residues, Lys21 and Tyr22, are essential for high-affinity binding. Unexpectedly, potassium ions traversing the channel from the inside confer voltage sensitivity to the Hui1 off-rate via Arg23, indicating that Lys21 is not in the pore. The 3D structure of Hui1 reveals a SAK1 fold, rationalizes KcsA inhibition, and validates the scaffold-based approach for isolation of high-affinity toxins for orphan receptors.

-	~~The entry 2n6b is ON HOLD until Paper Publication~~	+	Designer and natural peptide toxin blockers of the KcsA potassium channel identified by phage display.,Zhao R, Dai H, Mendelman N, Cuello LG, Chill JH, Goldstein SA Proc Natl Acad Sci U S A. 2015 Dec 1. pii: 201514728. PMID:26627718<ref>PMID:26627718</ref>

-	~~Authors: Mendelman~~, N.~~, Zhao, R., Goldstein,~~ S.A.~~N.,~~ Chill, J.H.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: NMR structure of the de-novo toxin Hui1~~	+	<div class="pdbe-citations 2n6b" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chill, J H]]
		+	[[Category: Goldstein, S A.N]]
	[[Category: Mendelman, N]]		[[Category: Mendelman, N]]
	[[Category: Zhao, R]]		[[Category: Zhao, R]]
-	[[Category: ~~Chill, J.H~~]]	+	[[Category: Potassium channel]]
-	[[Category: ~~Goldstein, S.A.N~~]]	+	[[Category: Shk]]
		+	[[Category: Toxin]]

OCA at 22:30, 30 November 2015

OCA — Mon, 30 Nov 2015 22:30:45 GMT

←Older revision		Revision as of 22:30, 30 November 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 2n6b is ON HOLD	+	The entry 2n6b is ON HOLD until Paper Publication

	Authors: Mendelman, N., Zhao, R., Goldstein, S.A.N., Chill, J.H.		Authors: Mendelman, N., Zhao, R., Goldstein, S.A.N., Chill, J.H.

OCA: Protected "2n6b" [edit=sysop:move=sysop]

OCA — Wed, 26 Aug 2015 08:02:34 GMT

Protected "2n6b" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:02, 26 August 2015

OCA: New page: '''Unreleased structure''' The entry 2n6b is ON HOLD Authors: Mendelman, N., Zhao, R., Goldstein, S.A.N., Chill, J.H. Description: NMR structure of the de-novo toxin Hui1 [[Category: U...

OCA — Wed, 26 Aug 2015 08:02:33 GMT

New page: '''Unreleased structure''' The entry 2n6b is ON HOLD Authors: Mendelman, N., Zhao, R., Goldstein, S.A.N., Chill, J.H. Description: NMR structure of the de-novo toxin Hui1 [[Category: U...

New page

'''Unreleased structure'''

The entry 2n6b is ON HOLD

Authors: Mendelman, N., Zhao, R., Goldstein, S.A.N., Chill, J.H.

Description: NMR structure of the de-novo toxin Hui1
[[Category: Unreleased Structures]]
[[Category: Mendelman, N]]
[[Category: Zhao, R]]
[[Category: Chill, J.H]]
[[Category: Goldstein, S.A.N]]