2n6t - Revision history

OCA at 07:04, 1 May 2024

OCA — Wed, 01 May 2024 07:04:01 GMT

←Older revision		Revision as of 07:04, 1 May 2024
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6t OCA], [https://pdbe.org/2n6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n6t RCSB], [https://www.ebi.ac.uk/pdbsum/2n6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6t OCA], [https://pdbe.org/2n6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n6t RCSB], [https://www.ebi.ac.uk/pdbsum/2n6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6t ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Neisseria meningitidis causes bacterial meningitis and septicemia. It evades the host complement system by upregulating expression of immune evasion factors in response to changes in temperature. RNA thermometers within mRNAs control expression of bacterial immune evasion factors, including CssA, in the 5'-untranslated region of the operon for capsule biosynthesis. We dissect the molecular mechanisms of thermoregulation and report the structure of the CssA thermometer. We show that the RNA thermometer acts as a rheostat, whose stability is optimized to respond in a small temperature range around 37 degrees C as occur within the upper airways during infection. Small increases in temperature gradually open up the structure to allow progressively increased access to the ribosome binding site. Even small changes in stability induced by mutations of imperfect base pairs, as in naturally occurring polymorphisms, shift the thermometer response outside of the desired temperature range, suggesting that its activity could be modulated by pharmacological intervention.
-
-	Structure and mechanism of a molecular rheostat, an RNA thermometer that modulates immune evasion by Neisseria meningitidis.,Barnwal RP, Loh E, Godin KS, Yip J, Lavender H, Tang CM, Varani G Nucleic Acids Res. 2016 Jul 1. pii: gkw584. PMID:27369378<ref>PMID:27369378</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2n6t" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 01:18, 28 December 2023

OCA — Thu, 28 Dec 2023 01:18:13 GMT

←Older revision		Revision as of 01:18, 28 December 2023
Line 1:		Line 1:

	==NMR Assignment and NMR Structure of CssA3 (top stem) of CssA thermometer==		==NMR Assignment and NMR Structure of CssA3 (top stem) of CssA thermometer==
-	<StructureSection load='2n6t' size='340' side='right'caption='[[2n6t~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='2n6t' size='340' side='right'caption='[[2n6t]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n6t]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N6T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n6t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N6T FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[2n6s\|2n6s]], [[2n6w\|2n6w]], [[2n6x\|2n6x]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6t OCA], [https://pdbe.org/2n6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n6t RCSB], [https://www.ebi.ac.uk/pdbsum/2n6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6t OCA], [https://pdbe.org/2n6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n6t RCSB], [https://www.ebi.ac.uk/pdbsum/2n6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6t ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Barnwal, R~~]]	+	[[Category: Neisseria meningitidis]]
-	[[Category: ~~Godin, K~~]]	+	[[Category: Barnwal R]]
-	[[Category: ~~Structural genomic~~]]	+	[[Category: Godin K]]
-	[[Category: Varani, G]]	+	[[Category: Varani G]]
-	~~[[Category: Pathogen]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Rna regulation]]~~	+
-	~~[[Category: Rna thermometer]]~~	+
-	~~[[Category: Ssgcid~~]]	+

OCA at 12:24, 23 March 2022

OCA — Wed, 23 Mar 2022 12:24:06 GMT

←Older revision		Revision as of 12:24, 23 March 2022
Line 3:		Line 3:
	<StructureSection load='2n6t' size='340' side='right'caption='[[2n6t]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='2n6t' size='340' side='right'caption='[[2n6t]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n6t]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2N6T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n6t]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N6T FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2n6s\|2n6s]], [[2n6w\|2n6w]], [[2n6x\|2n6x]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2n6s\|2n6s]], [[2n6w\|2n6w]], [[2n6x\|2n6x]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6t OCA], [~~http~~://pdbe.org/2n6t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2n6t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2n6t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6t ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6t OCA], [https://pdbe.org/2n6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n6t RCSB], [https://www.ebi.ac.uk/pdbsum/2n6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6t ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:02, 17 June 2020

OCA — Wed, 17 Jun 2020 11:02:53 GMT

←Older revision		Revision as of 11:02, 17 June 2020
Line 1:		Line 1:

	==NMR Assignment and NMR Structure of CssA3 (top stem) of CssA thermometer==		==NMR Assignment and NMR Structure of CssA3 (top stem) of CssA thermometer==
-	<StructureSection load='2n6t' size='340' side='right' caption='[[2n6t]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='2n6t' size='340' side='right'caption='[[2n6t]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n6t]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6T OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2N6T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n6t]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2N6T FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2n6s\|2n6s]], [[2n6w\|2n6w]], [[2n6x\|2n6x]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2n6s\|2n6s]], [[2n6w\|2n6w]], [[2n6x\|2n6x]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6t OCA], [http://pdbe.org/2n6t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n6t RCSB], [http://www.ebi.ac.uk/pdbsum/2n6t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6t ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6t OCA], [http://pdbe.org/2n6t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n6t RCSB], [http://www.ebi.ac.uk/pdbsum/2n6t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6t ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Barnwal, R]]		[[Category: Barnwal, R]]
	[[Category: Godin, K]]		[[Category: Godin, K]]

OCA at 08:39, 12 September 2018

OCA — Wed, 12 Sep 2018 08:39:56 GMT

←Older revision		Revision as of 08:39, 12 September 2018
Line 22:		Line 22:
	[[Category: Barnwal, R]]		[[Category: Barnwal, R]]
	[[Category: Godin, K]]		[[Category: Godin, K]]
		+	[[Category: Structural genomic]]
	[[Category: Varani, G]]		[[Category: Varani, G]]
	[[Category: Pathogen]]		[[Category: Pathogen]]
Line 27:		Line 28:
	[[Category: Rna regulation]]		[[Category: Rna regulation]]
	[[Category: Rna thermometer]]		[[Category: Rna thermometer]]
		+	[[Category: Ssgcid]]

OCA at 04:02, 4 August 2016

OCA — Thu, 04 Aug 2016 04:02:00 GMT

←Older revision		Revision as of 04:02, 4 August 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 2n6t is ~~ON HOLD~~	+	==NMR Assignment and NMR Structure of CssA3 (top stem) of CssA thermometer==
		+	<StructureSection load='2n6t' size='340' side='right' caption='[[2n6t]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2n6t]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N6T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N6T FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2n6s\|2n6s]], [[2n6w\|2n6w]], [[2n6x\|2n6x]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n6t OCA], [http://pdbe.org/2n6t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n6t RCSB], [http://www.ebi.ac.uk/pdbsum/2n6t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n6t ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Neisseria meningitidis causes bacterial meningitis and septicemia. It evades the host complement system by upregulating expression of immune evasion factors in response to changes in temperature. RNA thermometers within mRNAs control expression of bacterial immune evasion factors, including CssA, in the 5'-untranslated region of the operon for capsule biosynthesis. We dissect the molecular mechanisms of thermoregulation and report the structure of the CssA thermometer. We show that the RNA thermometer acts as a rheostat, whose stability is optimized to respond in a small temperature range around 37 degrees C as occur within the upper airways during infection. Small increases in temperature gradually open up the structure to allow progressively increased access to the ribosome binding site. Even small changes in stability induced by mutations of imperfect base pairs, as in naturally occurring polymorphisms, shift the thermometer response outside of the desired temperature range, suggesting that its activity could be modulated by pharmacological intervention.

-	~~Authors: Barnwal~~, R., Godin, K., ~~Varani~~, G.	+	Structure and mechanism of a molecular rheostat, an RNA thermometer that modulates immune evasion by Neisseria meningitidis.,Barnwal RP, Loh E, Godin KS, Yip J, Lavender H, Tang CM, Varani G Nucleic Acids Res. 2016 Jul 1. pii: gkw584. PMID:27369378<ref>PMID:27369378</ref>

-	~~Description: NMR Assignment and NMR Structure~~ of ~~CssA3 (top stem)~~ of ~~CssA thermometer~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Varani, G]]~~	+	<div class="pdbe-citations 2n6t" style="background-color:#fffaf0;"></div>
-	~~[[Category: Godin, K]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Barnwal, R]]		[[Category: Barnwal, R]]
		+	[[Category: Godin, K]]
		+	[[Category: Varani, G]]
		+	[[Category: Pathogen]]
		+	[[Category: Rna]]
		+	[[Category: Rna regulation]]
		+	[[Category: Rna thermometer]]

OCA: Protected "2n6t" [edit=sysop:move=sysop]

OCA — Wed, 09 Sep 2015 05:25:09 GMT

Protected "2n6t" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:25, 9 September 2015

OCA: New page: '''Unreleased structure''' The entry 2n6t is ON HOLD Authors: Barnwal, R., Godin, K., Varani, G. Description: NMR Assignment and NMR Structure of CssA3 (top stem) of CssA thermometer [...

OCA — Wed, 09 Sep 2015 05:25:08 GMT

New page: '''Unreleased structure''' The entry 2n6t is ON HOLD Authors: Barnwal, R., Godin, K., Varani, G. Description: NMR Assignment and NMR Structure of CssA3 (top stem) of CssA thermometer [...

New page

'''Unreleased structure'''

The entry 2n6t is ON HOLD

Authors: Barnwal, R., Godin, K., Varani, G.

Description: NMR Assignment and NMR Structure of CssA3 (top stem) of CssA thermometer
[[Category: Unreleased Structures]]
[[Category: Varani, G]]
[[Category: Godin, K]]
[[Category: Barnwal, R]]