2n8p - Revision history

OCA at 07:04, 1 May 2024

OCA — Wed, 01 May 2024 07:04:14 GMT

←Older revision		Revision as of 07:04, 1 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2n8p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8P FirstGlance]. <br>		<table><tr><td colspan='2'>[[2n8p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8p OCA], [https://pdbe.org/2n8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8p RCSB], [https://www.ebi.ac.uk/pdbsum/2n8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8p ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8p OCA], [https://pdbe.org/2n8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8p RCSB], [https://www.ebi.ac.uk/pdbsum/2n8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A4UVR2_9LACT A4UVR2_9LACT]		[https://www.uniprot.org/uniprot/A4UVR2_9LACT A4UVR2_9LACT]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Lacticin Q (LnqQ) and aureocin A53 (AucA) are leaderless bacteriocins from Lactococcus lactis QU5 and Staphylococcus aureus A53, respectively. These bacteriocins are characterized by the absence of an N-terminal leader sequence and are active against a broad range of Gram-positive bacteria. LnqQ and AucA consist of 53 and 51 amino acids, respectively, and have 47% identical sequences. In this study, their three-dimensional structures were elucidated using solution nuclear magnetic resonance and were shown to consist of four alpha-helices that assume a very similar compact, globular overall fold (root-mean-square deviation of 1.7 A) with a highly cationic surface and a hydrophobic core. The structures of LnqQ and AucA resemble the shorter two-component leaderless bacteriocins, enterocins 7A and 7B, despite having low levels of sequence identity. Homology modeling revealed that the observed structural motif may be shared among leaderless bacteriocins with broad-spectrum activity against Gram-positive organisms. The elucidated structures of LnqQ and AucA also exhibit some resemblance to circular bacteriocins. Despite their similar overall fold, inhibition studies showed that LnqQ and AucA have different antimicrobial potency against the Gram-positive strains tested, suggesting that sequence disparities play a crucial role in their mechanisms of action.
-
-	Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity.,Acedo JZ, van Belkum MJ, Lohans CT, Towle KM, Miskolzie M, Vederas JC Biochemistry. 2016 Feb 2;55(4):733-42. doi: 10.1021/acs.biochem.5b01306. Epub, 2016 Jan 15. PMID:26771761<ref>PMID:26771761</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2n8p" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:23, 15 March 2023

OCA — Wed, 15 Mar 2023 10:23:29 GMT

←Older revision		Revision as of 10:23, 15 March 2023
Line 1:		Line 1:

	==Solution Structure of Lacticin Q==		==Solution Structure of Lacticin Q==
-	<StructureSection load='2n8p' size='340' side='right'caption='[[2n8p~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2n8p' size='340' side='right'caption='[[2n8p]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n8p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacterium_lactis"_lister_1873 "bacterium~~ lactis~~" lister 1873~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n8p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8P FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2n8o\|2n8o]]</div></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8p OCA], [https://pdbe.org/2n8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8p RCSB], [https://www.ebi.ac.uk/pdbsum/2n8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8p ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lnqQ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 "Bacterium lactis" Lister 1873])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8p OCA], [https://pdbe.org/2n8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8p RCSB], [https://www.ebi.ac.uk/pdbsum/2n8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8p ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A4UVR2_9LACT A4UVR2_9LACT]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacterium~~ lactis ~~lister 1873~~]]	+	[[Category: Lactococcus lactis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Acedo~~, J Z]]~~	+	[[Category: Acedo JZ]]
-	~~[[Category: Belkum, M J.van~~]]	+	[[Category: Lohans CT]]
-	[[Category: Lohans~~, C T~~]]	+	[[Category: Miskolzie M]]
-	[[Category: Miskolzie, M]]	+	[[Category: Towle KM]]
-	[[Category: Towle~~, K M~~]]	+	[[Category: Vederas JC]]
-	[[Category: Vederas~~, J C~~]]	+	[[Category: Van Belkum MJ]]
-	[[Category: ~~Antimicrobial protein]]~~	+
-	~~[[Category: Leaderless bacteriocin~~]]	+

OCA at 15:42, 8 June 2021

OCA — Tue, 08 Jun 2021 15:42:04 GMT

←Older revision		Revision as of 15:42, 8 June 2021
Line 1:		Line 1:

	==Solution Structure of Lacticin Q==		==Solution Structure of Lacticin Q==
-	<StructureSection load='2n8p' size='340' side='right' caption='[[2n8p]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2n8p' size='340' side='right'caption='[[2n8p]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n8p]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacterium_lactis"_lister_1873 "bacterium lactis" lister 1873]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2N8P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n8p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_lactis"_lister_1873 "bacterium lactis" lister 1873]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N8P FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2n8o\|2n8o]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2n8o\|2n8o]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lnqQ ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 "Bacterium lactis" Lister 1873])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lnqQ ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 "Bacterium lactis" Lister 1873])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2n8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8p OCA], [~~http~~://pdbe.org/2n8p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2n8p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2n8p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8p OCA], [https://pdbe.org/2n8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n8p RCSB], [https://www.ebi.ac.uk/pdbsum/2n8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8p ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Bacterium lactis lister 1873]]		[[Category: Bacterium lactis lister 1873]]
		+	[[Category: Large Structures]]
	[[Category: Acedo, J Z]]		[[Category: Acedo, J Z]]
	[[Category: Belkum, M J.van]]		[[Category: Belkum, M J.van]]

OCA at 17:18, 15 August 2018

OCA — Wed, 15 Aug 2018 17:18:43 GMT

←Older revision		Revision as of 17:18, 15 August 2018
Line 3:		Line 3:
	<StructureSection load='2n8p' size='340' side='right' caption='[[2n8p]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2n8p' size='340' side='right' caption='[[2n8p]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n8p]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N8P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n8p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_lactis"_lister_1873 "bacterium lactis" lister 1873]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N8P FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2n8o\|2n8o]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2n8o\|2n8o]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8p OCA], [http://pdbe.org/2n8p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n8p RCSB], [http://www.ebi.ac.uk/pdbsum/2n8p PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lnqQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 "Bacterium lactis" Lister 1873])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8p OCA], [http://pdbe.org/2n8p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n8p RCSB], [http://www.ebi.ac.uk/pdbsum/2n8p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n8p ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacterium lactis lister 1873]]
	[[Category: Acedo, J Z]]		[[Category: Acedo, J Z]]
	[[Category: Belkum, M J.van]]		[[Category: Belkum, M J.van]]

OCA at 02:42, 21 February 2016

OCA — Sun, 21 Feb 2016 02:42:04 GMT

←Older revision		Revision as of 02:42, 21 February 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 2n8p is ~~ON HOLD until Paper~~ Publication	+	==Solution Structure of Lacticin Q==
		+	<StructureSection load='2n8p' size='340' side='right' caption='[[2n8p]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2n8p]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N8P FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2n8o\|2n8o]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n8p OCA], [http://pdbe.org/2n8p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n8p RCSB], [http://www.ebi.ac.uk/pdbsum/2n8p PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lacticin Q (LnqQ) and aureocin A53 (AucA) are leaderless bacteriocins from Lactococcus lactis QU5 and Staphylococcus aureus A53, respectively. These bacteriocins are characterized by the absence of an N-terminal leader sequence and are active against a broad range of Gram-positive bacteria. LnqQ and AucA consist of 53 and 51 amino acids, respectively, and have 47% identical sequences. In this study, their three-dimensional structures were elucidated using solution nuclear magnetic resonance and were shown to consist of four alpha-helices that assume a very similar compact, globular overall fold (root-mean-square deviation of 1.7 A) with a highly cationic surface and a hydrophobic core. The structures of LnqQ and AucA resemble the shorter two-component leaderless bacteriocins, enterocins 7A and 7B, despite having low levels of sequence identity. Homology modeling revealed that the observed structural motif may be shared among leaderless bacteriocins with broad-spectrum activity against Gram-positive organisms. The elucidated structures of LnqQ and AucA also exhibit some resemblance to circular bacteriocins. Despite their similar overall fold, inhibition studies showed that LnqQ and AucA have different antimicrobial potency against the Gram-positive strains tested, suggesting that sequence disparities play a crucial role in their mechanisms of action.

-	~~Authors:~~ Acedo~~, J.Z.~~, van Belkum~~, M.J.~~, Lohans~~, C.T.~~, Towle~~, K.M.~~, Miskolzie, M., Vederas, ~~J.C~~.	+	Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity.,Acedo JZ, van Belkum MJ, Lohans CT, Towle KM, Miskolzie M, Vederas JC Biochemistry. 2016 Feb 2;55(4):733-42. doi: 10.1021/acs.biochem.5b01306. Epub, 2016 Jan 15. PMID:26771761<ref>PMID:26771761</ref>

-	~~Description: Solution Structure~~ of ~~Lacticin Q~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Lohans~~, C.T]]	+	<div class="pdbe-citations 2n8p" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Van Belkum~~, ~~M.J~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Acedo, J Z]]
		+	[[Category: Belkum, M J.van]]
		+	[[Category: Lohans, C T]]
	[[Category: Miskolzie, M]]		[[Category: Miskolzie, M]]
-	[[Category: ~~Acedo~~, ~~J.Z~~]]	+	[[Category: Towle, K M]]
-	[[Category: ~~Towle~~, ~~K.M~~]]	+	[[Category: Vederas, J C]]
-	[[Category: ~~Vederas, J.C~~]]	+	[[Category: Antimicrobial protein]]
		+	[[Category: Leaderless bacteriocin]]

OCA at 06:40, 23 December 2015

OCA — Wed, 23 Dec 2015 06:40:33 GMT

←Older revision		Revision as of 06:40, 23 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 2n8p is ON HOLD	+	The entry 2n8p is ON HOLD until Paper Publication

	Authors: Acedo, J.Z., van Belkum, M.J., Lohans, C.T., Towle, K.M., Miskolzie, M., Vederas, J.C.		Authors: Acedo, J.Z., van Belkum, M.J., Lohans, C.T., Towle, K.M., Miskolzie, M., Vederas, J.C.

OCA: Protected "2n8p" [edit=sysop:move=sysop]

OCA — Mon, 30 Nov 2015 22:34:30 GMT

Protected "2n8p" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:34, 30 November 2015

OCA: New page: '''Unreleased structure''' The entry 2n8p is ON HOLD Authors: Acedo, J.Z., van Belkum, M.J., Lohans, C.T., Towle, K.M., Miskolzie, M., Vederas, J.C. Description: Solution Structure of ...

OCA — Mon, 30 Nov 2015 22:34:29 GMT

New page: '''Unreleased structure''' The entry 2n8p is ON HOLD Authors: Acedo, J.Z., van Belkum, M.J., Lohans, C.T., Towle, K.M., Miskolzie, M., Vederas, J.C. Description: Solution Structure of ...

New page

'''Unreleased structure'''

The entry 2n8p is ON HOLD

Authors: Acedo, J.Z., van Belkum, M.J., Lohans, C.T., Towle, K.M., Miskolzie, M., Vederas, J.C.

Description: Solution Structure of Lacticin Q
[[Category: Unreleased Structures]]
[[Category: Lohans, C.T]]
[[Category: Van Belkum, M.J]]
[[Category: Miskolzie, M]]
[[Category: Acedo, J.Z]]
[[Category: Towle, K.M]]
[[Category: Vederas, J.C]]