2n9p - Revision history

OCA at 10:24, 15 March 2023

OCA — Wed, 15 Mar 2023 10:24:55 GMT

←Older revision		Revision as of 10:24, 15 March 2023
Line 1:		Line 1:

	==Solution structure of RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain==		==Solution structure of RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain==
-	<StructureSection load='2n9p' size='340' side='right'caption='[[2n9p~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2n9p' size='340' side='right'caption='[[2n9p]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n9p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N9P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n9p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N9P FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2n9o\|2n9o]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RNF126 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), BAG6, BAT3, G3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9p OCA], [https://pdbe.org/2n9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n9p RCSB], [https://www.ebi.ac.uk/pdbsum/2n9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9p ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9p OCA], [https://pdbe.org/2n9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n9p RCSB], [https://www.ebi.ac.uk/pdbsum/2n9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RN126_HUMAN RN126_HUMAN]] E3 ubiquitin-protein ligase that regulates several biological processes through ubiquitination of various target proteins. Depending on the associated E2 ligase, mediates 'Lys-48'- and 'Lys-63'-linked polyubiquitination of substrates. Through their polyubiquitination, may play a role in the endosomal sorting and degradation of several membrane receptors including EGFR, FLT3, MET and CXCR4. May also be part of a BAG6-dependent quality control process ensuring that proteins of the secretory pathway that are mislocalized to the cytosol are degraded by the proteasome. May provide the ubiquitin ligase activity associated with the BAG6 complex and be responsible for ubiquitination of the mislocalized proteins and their targeting to the proteasome (PubMed:24981174). May also play a role in the endosomal recycling of IGF2R, the cation-independent mannose-6-phosphate receptor (PubMed:24275455). By ubiquitinating CDKN1A/p21 and targeting it for degradation, may also promote cell proliferation (PubMed:23026136). May monoubiquitinate AICDA (PubMed:23277564).[UniProtKB:Q91YL2]<ref>PMID:23277564</ref> <ref>PMID:24275455</ref> <ref>PMID:24981174</ref> <ref>PMID:23026136</ref> [[https://www.uniprot.org/uniprot/BAG6_HUMAN BAG6_HUMAN]] Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane.<ref>PMID:14960581</ref> <ref>PMID:17403783</ref> <ref>PMID:18055229</ref> <ref>PMID:18765639</ref> <ref>PMID:18852879</ref> <ref>PMID:20516149</ref> <ref>PMID:20676083</ref> Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).<ref>PMID:14960581</ref> <ref>PMID:17403783</ref> <ref>PMID:18055229</ref> <ref>PMID:18765639</ref> <ref>PMID:18852879</ref> <ref>PMID:20516149</ref> <ref>PMID:20676083</ref> Can be released from tumor and dendritic cells in membrane vesicles or exosomes, and engage NCR3 thereby promoting natural killer cell (NK) activation and cytotoxicity.<ref>PMID:14960581</ref> <ref>PMID:17403783</ref> <ref>PMID:18055229</ref> <ref>PMID:18765639</ref> <ref>PMID:18852879</ref> <ref>PMID:20516149</ref> <ref>PMID:20676083</ref>	+	[https://www.uniprot.org/uniprot/RN126_HUMAN RN126_HUMAN] E3 ubiquitin-protein ligase that regulates several biological processes through ubiquitination of various target proteins. Depending on the associated E2 ligase, mediates 'Lys-48'- and 'Lys-63'-linked polyubiquitination of substrates. Through their polyubiquitination, may play a role in the endosomal sorting and degradation of several membrane receptors including EGFR, FLT3, MET and CXCR4. May also be part of a BAG6-dependent quality control process ensuring that proteins of the secretory pathway that are mislocalized to the cytosol are degraded by the proteasome. May provide the ubiquitin ligase activity associated with the BAG6 complex and be responsible for ubiquitination of the mislocalized proteins and their targeting to the proteasome (PubMed:24981174). May also play a role in the endosomal recycling of IGF2R, the cation-independent mannose-6-phosphate receptor (PubMed:24275455). By ubiquitinating CDKN1A/p21 and targeting it for degradation, may also promote cell proliferation (PubMed:23026136). May monoubiquitinate AICDA (PubMed:23277564).[UniProtKB:Q91YL2]<ref>PMID:23277564</ref> <ref>PMID:24275455</ref> <ref>PMID:24981174</ref> <ref>PMID:23026136</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Isaacson~~, R L~~]]	+	[[Category: Isaacson RL]]
-	[[Category: Krysztofinska~~, E M~~]]	+	[[Category: Krysztofinska EM]]
-	[[Category: Martinez-Lumbreras, S]]	+	[[Category: Martinez-Lumbreras S]]
-	[[Category: Thapaliya, A]]	+	[[Category: Thapaliya A]]
-	~~[[Category: E3 ligase]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Zinc finger~~]]	+

OCA at 15:42, 8 June 2021

OCA — Tue, 08 Jun 2021 15:42:51 GMT

←Older revision		Revision as of 15:42, 8 June 2021
Line 1:		Line 1:

	==Solution structure of RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain==		==Solution structure of RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain==
-	<StructureSection load='2n9p' size='340' side='right' caption='[[2n9p]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2n9p' size='340' side='right'caption='[[2n9p]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n9p]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2N9P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n9p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N9P FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2n9o\|2n9o]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2n9o\|2n9o]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RNF126 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), BAG6, BAT3, G3 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RNF126 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), BAG6, BAT3, G3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2n9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9p OCA], [~~http~~://pdbe.org/2n9p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2n9p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2n9p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9p OCA], [https://pdbe.org/2n9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n9p RCSB], [https://www.ebi.ac.uk/pdbsum/2n9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RN126_HUMAN RN126_HUMAN]] E3 ubiquitin-protein ligase that regulates several biological processes through ubiquitination of various target proteins. Depending on the associated E2 ligase, mediates 'Lys-48'- and 'Lys-63'-linked polyubiquitination of substrates. Through their polyubiquitination, may play a role in the endosomal sorting and degradation of several membrane receptors including EGFR, FLT3, MET and CXCR4. May also be part of a BAG6-dependent quality control process ensuring that proteins of the secretory pathway that are mislocalized to the cytosol are degraded by the proteasome. May provide the ubiquitin ligase activity associated with the BAG6 complex and be responsible for ubiquitination of the mislocalized proteins and their targeting to the proteasome (PubMed:24981174). May also play a role in the endosomal recycling of IGF2R, the cation-independent mannose-6-phosphate receptor (PubMed:24275455). By ubiquitinating CDKN1A/p21 and targeting it for degradation, may also promote cell proliferation (PubMed:23026136). May monoubiquitinate AICDA (PubMed:23277564).[UniProtKB:Q91YL2]<ref>PMID:23277564</ref> <ref>PMID:24275455</ref> <ref>PMID:24981174</ref> <ref>PMID:23026136</ref> [[~~http~~://www.uniprot.org/uniprot/BAG6_HUMAN BAG6_HUMAN]] Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane.<ref>PMID:14960581</ref> <ref>PMID:17403783</ref> <ref>PMID:18055229</ref> <ref>PMID:18765639</ref> <ref>PMID:18852879</ref> <ref>PMID:20516149</ref> <ref>PMID:20676083</ref> Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).<ref>PMID:14960581</ref> <ref>PMID:17403783</ref> <ref>PMID:18055229</ref> <ref>PMID:18765639</ref> <ref>PMID:18852879</ref> <ref>PMID:20516149</ref> <ref>PMID:20676083</ref> Can be released from tumor and dendritic cells in membrane vesicles or exosomes, and engage NCR3 thereby promoting natural killer cell (NK) activation and cytotoxicity.<ref>PMID:14960581</ref> <ref>PMID:17403783</ref> <ref>PMID:18055229</ref> <ref>PMID:18765639</ref> <ref>PMID:18852879</ref> <ref>PMID:20516149</ref> <ref>PMID:20676083</ref>	+	[[https://www.uniprot.org/uniprot/RN126_HUMAN RN126_HUMAN]] E3 ubiquitin-protein ligase that regulates several biological processes through ubiquitination of various target proteins. Depending on the associated E2 ligase, mediates 'Lys-48'- and 'Lys-63'-linked polyubiquitination of substrates. Through their polyubiquitination, may play a role in the endosomal sorting and degradation of several membrane receptors including EGFR, FLT3, MET and CXCR4. May also be part of a BAG6-dependent quality control process ensuring that proteins of the secretory pathway that are mislocalized to the cytosol are degraded by the proteasome. May provide the ubiquitin ligase activity associated with the BAG6 complex and be responsible for ubiquitination of the mislocalized proteins and their targeting to the proteasome (PubMed:24981174). May also play a role in the endosomal recycling of IGF2R, the cation-independent mannose-6-phosphate receptor (PubMed:24275455). By ubiquitinating CDKN1A/p21 and targeting it for degradation, may also promote cell proliferation (PubMed:23026136). May monoubiquitinate AICDA (PubMed:23277564).[UniProtKB:Q91YL2]<ref>PMID:23277564</ref> <ref>PMID:24275455</ref> <ref>PMID:24981174</ref> <ref>PMID:23026136</ref> [[https://www.uniprot.org/uniprot/BAG6_HUMAN BAG6_HUMAN]] Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane.<ref>PMID:14960581</ref> <ref>PMID:17403783</ref> <ref>PMID:18055229</ref> <ref>PMID:18765639</ref> <ref>PMID:18852879</ref> <ref>PMID:20516149</ref> <ref>PMID:20676083</ref> Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).<ref>PMID:14960581</ref> <ref>PMID:17403783</ref> <ref>PMID:18055229</ref> <ref>PMID:18765639</ref> <ref>PMID:18852879</ref> <ref>PMID:20516149</ref> <ref>PMID:20676083</ref> Can be released from tumor and dendritic cells in membrane vesicles or exosomes, and engage NCR3 thereby promoting natural killer cell (NK) activation and cytotoxicity.<ref>PMID:14960581</ref> <ref>PMID:17403783</ref> <ref>PMID:18055229</ref> <ref>PMID:18765639</ref> <ref>PMID:18852879</ref> <ref>PMID:20516149</ref> <ref>PMID:20676083</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[BAG ~~protein~~\|BAG ~~protein~~]]	+	*[[BAG family proteins 3D structures\|BAG family proteins 3D structures]]
-	*[[Ubiquitin protein ligase\|Ubiquitin protein ligase]]	+	*[[Ubiquitin protein ligase 3D structures\|Ubiquitin protein ligase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Isaacson, R L]]		[[Category: Isaacson, R L]]
	[[Category: Krysztofinska, E M]]		[[Category: Krysztofinska, E M]]

OCA at 17:32, 15 August 2018

OCA — Wed, 15 Aug 2018 17:32:18 GMT

←Older revision		Revision as of 17:32, 15 August 2018
Line 3:		Line 3:
	<StructureSection load='2n9p' size='340' side='right' caption='[[2n9p]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2n9p' size='340' side='right' caption='[[2n9p]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n9p]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N9P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n9p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N9P FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2n9o\|2n9o]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2n9o\|2n9o]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9p OCA], [http://pdbe.org/2n9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n9p RCSB], [http://www.ebi.ac.uk/pdbsum/2n9p PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RNF126 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), BAG6, BAT3, G3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9p OCA], [http://pdbe.org/2n9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n9p RCSB], [http://www.ebi.ac.uk/pdbsum/2n9p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 2n9p" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2n9p" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[BAG protein\|BAG protein]]
		+	*[[Ubiquitin protein ligase\|Ubiquitin protein ligase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Isaacson, R L]]		[[Category: Isaacson, R L]]
	[[Category: Krysztofinska, E M]]		[[Category: Krysztofinska, E M]]

OCA at 08:34, 1 June 2016

OCA — Wed, 01 Jun 2016 08:34:10 GMT

←Older revision		Revision as of 08:34, 1 June 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 2n9p is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Solution structure of RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain==
		+	<StructureSection load='2n9p' size='340' side='right' caption='[[2n9p]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2n9p]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N9P FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2n9o\|2n9o]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9p OCA], [http://pdbe.org/2n9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n9p RCSB], [http://www.ebi.ac.uk/pdbsum/2n9p PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RN126_HUMAN RN126_HUMAN]] E3 ubiquitin-protein ligase that regulates several biological processes through ubiquitination of various target proteins. Depending on the associated E2 ligase, mediates 'Lys-48'- and 'Lys-63'-linked polyubiquitination of substrates. Through their polyubiquitination, may play a role in the endosomal sorting and degradation of several membrane receptors including EGFR, FLT3, MET and CXCR4. May also be part of a BAG6-dependent quality control process ensuring that proteins of the secretory pathway that are mislocalized to the cytosol are degraded by the proteasome. May provide the ubiquitin ligase activity associated with the BAG6 complex and be responsible for ubiquitination of the mislocalized proteins and their targeting to the proteasome (PubMed:24981174). May also play a role in the endosomal recycling of IGF2R, the cation-independent mannose-6-phosphate receptor (PubMed:24275455). By ubiquitinating CDKN1A/p21 and targeting it for degradation, may also promote cell proliferation (PubMed:23026136). May monoubiquitinate AICDA (PubMed:23277564).[UniProtKB:Q91YL2]<ref>PMID:23277564</ref> <ref>PMID:24275455</ref> <ref>PMID:24981174</ref> <ref>PMID:23026136</ref> [[http://www.uniprot.org/uniprot/BAG6_HUMAN BAG6_HUMAN]] Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane.<ref>PMID:14960581</ref> <ref>PMID:17403783</ref> <ref>PMID:18055229</ref> <ref>PMID:18765639</ref> <ref>PMID:18852879</ref> <ref>PMID:20516149</ref> <ref>PMID:20676083</ref> Involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).<ref>PMID:14960581</ref> <ref>PMID:17403783</ref> <ref>PMID:18055229</ref> <ref>PMID:18765639</ref> <ref>PMID:18852879</ref> <ref>PMID:20516149</ref> <ref>PMID:20676083</ref> Can be released from tumor and dendritic cells in membrane vesicles or exosomes, and engage NCR3 thereby promoting natural killer cell (NK) activation and cytotoxicity.<ref>PMID:14960581</ref> <ref>PMID:17403783</ref> <ref>PMID:18055229</ref> <ref>PMID:18765639</ref> <ref>PMID:18852879</ref> <ref>PMID:20516149</ref> <ref>PMID:20676083</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RNF126 is an E3 ubiquitin ligase that collaborates with the BAG6 sortase complex to ubiquitinate hydrophobic substrates in the cytoplasm that are destined for proteasomal recycling. Composed of a trimeric complex of BAG6, TRC35 and UBL4A the BAG6 sortase is also associated with SGTA, a co-chaperone from which it can obtain hydrophobic substrates. Here we solve the solution structure of the RNF126 zinc finger domain in complex with the BAG6 UBL domain. We also characterise an interaction between RNF126 and UBL4A and analyse the competition between SGTA and RNF126 for the N-terminal BAG6 binding site. This work sheds light on the sorting mechanism of the BAG6 complex and its accessory proteins which, together, decide the fate of stray hydrophobic proteins in the aqueous cytoplasm.

-	~~Authors: Martinez-Lumbreras~~, S., Krysztofinska, ~~E.M.~~, Thapaliya, A., ~~Isaacson~~, R.L.	+	Structural and functional insights into the E3 ligase, RNF126.,Krysztofinska EM, Martinez-Lumbreras S, Thapaliya A, Evans NJ, High S, Isaacson RL Sci Rep. 2016 May 19;6:26433. doi: 10.1038/srep26433. PMID:27193484<ref>PMID:27193484</ref>

-	~~Description: Solution structure~~ of ~~RNF126 N~~-~~terminal zinc finger domain in complex with BAG6 Ubiquitin~~-~~like domain~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Thapaliya, A]]~~	+	<div class="pdbe-citations 2n9p" style="background-color:#fffaf0;"></div>
-	[[Category: Isaacson, R.L]]	+	== References ==
-	[[Category: Krysztofinska, E.M]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Isaacson, R L]]
		+	[[Category: Krysztofinska, E M]]
	[[Category: Martinez-Lumbreras, S]]		[[Category: Martinez-Lumbreras, S]]
		+	[[Category: Thapaliya, A]]
		+	[[Category: E3 ligase]]
		+	[[Category: Ligase]]
		+	[[Category: Zinc finger]]

OCA at 20:00, 10 May 2016

OCA — Tue, 10 May 2016 20:00:23 GMT

←Older revision		Revision as of 20:00, 10 May 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 2n9p is ON HOLD	+	The entry 2n9p is ON HOLD until Paper Publication

	Authors: Martinez-Lumbreras, S., Krysztofinska, E.M., Thapaliya, A., Isaacson, R.L.		Authors: Martinez-Lumbreras, S., Krysztofinska, E.M., Thapaliya, A., Isaacson, R.L.

OCA: Protected "2n9p" [edit=sysop:move=sysop]

OCA — Wed, 09 Dec 2015 07:48:19 GMT

Protected "2n9p" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:48, 9 December 2015

OCA: New page: '''Unreleased structure''' The entry 2n9p is ON HOLD Authors: Martinez-Lumbreras, S., Krysztofinska, E.M., Thapaliya, A., Isaacson, R.L. Description: Solution structure of RNF126 N-ter...

OCA — Wed, 09 Dec 2015 07:48:18 GMT

New page: '''Unreleased structure''' The entry 2n9p is ON HOLD Authors: Martinez-Lumbreras, S., Krysztofinska, E.M., Thapaliya, A., Isaacson, R.L. Description: Solution structure of RNF126 N-ter...

New page

'''Unreleased structure'''

The entry 2n9p is ON HOLD

Authors: Martinez-Lumbreras, S., Krysztofinska, E.M., Thapaliya, A., Isaacson, R.L.

Description: Solution structure of RNF126 N-terminal zinc finger domain in complex with BAG6 Ubiquitin-like domain
[[Category: Unreleased Structures]]
[[Category: Thapaliya, A]]
[[Category: Isaacson, R.L]]
[[Category: Krysztofinska, E.M]]
[[Category: Martinez-Lumbreras, S]]