2n9x - Revision history

OCA at 07:04, 1 May 2024

OCA — Wed, 01 May 2024 07:04:25 GMT

←Older revision		Revision as of 07:04, 1 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2n9x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N9X FirstGlance]. <br>		<table><tr><td colspan='2'>[[2n9x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N9X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9x OCA], [https://pdbe.org/2n9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n9x RCSB], [https://www.ebi.ac.uk/pdbsum/2n9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9x ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9x OCA], [https://pdbe.org/2n9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n9x RCSB], [https://www.ebi.ac.uk/pdbsum/2n9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN] Involved in formation of autophagosomal vacuoles (autophagosomes).		[https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN] Involved in formation of autophagosomal vacuoles (autophagosomes).
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Mitophagy is a fundamental process that determines mitochondrial quality and homeostasis. Several mitophagy receptors, including the newly identified FUNDC1, mediate selective removal of damaged or superfluous mitochondria through their specific interaction with LC3. However, the precise mechanism by which this interaction is regulated to initiate mitophagy is not understood. Here, we report the solution structure of LC3 in complex with a peptide containing the FUNDC1 LC3-interacting region (LIR) motif. The structure reveals a noncanonical LC3-LIR binding conformation, in which the third LIR residue (Val20) is also inserted into the hydrophobic pocket of LC3, together with the conserved residues Tyr18 and Leu21. This enables Tyr18 to be positioned near Asp19 of LC3, and thus phosphorylation of Tyr18 significantly weakens the binding affinity due to electrostatic repulsion. Functional analysis revealed that mitochondrial targeting of the LIR-containing cytosolic portion of FUNDC1 is necessary and sufficient to initiate mitophagy when Tyr18 is unphosphorylated, even in the absence of mitochondrial fragmentation. Thus, we demonstrated that phosphorylation of Tyr18 of FUNDC1 serves as a molecular switch for mitophagy. This may represent a novel target for therapeutic intervention.
-
-	Structural basis for the phosphorylation of FUNDC1 LIR as a molecular switch of mitophagy.,Kuang Y, Ma K, Zhou C, Ding P, Zhu Y, Chen Q, Xia B Autophagy. 2016 Dec;12(12):2363-2373. Epub 2016 Sep 21. PMID:27653272<ref>PMID:27653272</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2n9x" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:25, 15 March 2023

OCA — Wed, 15 Mar 2023 10:25:04 GMT

←Older revision		Revision as of 10:25, 15 March 2023
Line 1:		Line 1:

	==LC3 FUNDC1 complex structure==		==LC3 FUNDC1 complex structure==
-	<StructureSection load='2n9x' size='340' side='right'caption='[[2n9x~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2n9x' size='340' side='right'caption='[[2n9x]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n9x]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N9X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n9x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N9X FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9x OCA], [https://pdbe.org/2n9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n9x RCSB], [https://www.ebi.ac.uk/pdbsum/2n9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9x ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9x OCA], [https://pdbe.org/2n9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n9x RCSB], [https://www.ebi.ac.uk/pdbsum/2n9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes). ~~[[https://www.uniprot.org/uniprot/FUND1_HUMAN FUND1_HUMAN]] Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.<ref>PMID:22267086</ref>~~	+	[https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN] Involved in formation of autophagosomal vacuoles (autophagosomes).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kuang, Y]]	+	[[Category: Kuang Y]]
-	[[Category: Xia, B]]	+	[[Category: Xia B]]
-	~~[[Category: Protein binding-peptide complex]]~~	+
-	~~[[Category: Protein/peptide~~]]	+

OCA at 15:42, 8 June 2021

OCA — Tue, 08 Jun 2021 15:42:57 GMT

←Older revision		Revision as of 15:42, 8 June 2021
Line 3:		Line 3:
	<StructureSection load='2n9x' size='340' side='right'caption='[[2n9x]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2n9x' size='340' side='right'caption='[[2n9x]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2n9x]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2N9X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2n9x]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N9X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2n9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9x OCA], [~~http~~://pdbe.org/2n9x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2n9x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2n9x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9x ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9x OCA], [https://pdbe.org/2n9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n9x RCSB], [https://www.ebi.ac.uk/pdbsum/2n9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes). [[~~http~~://www.uniprot.org/uniprot/FUND1_HUMAN FUND1_HUMAN]] Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.<ref>PMID:22267086</ref>	+	[[https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes). [[https://www.uniprot.org/uniprot/FUND1_HUMAN FUND1_HUMAN]] Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.<ref>PMID:22267086</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:41, 21 August 2019

OCA — Wed, 21 Aug 2019 07:41:46 GMT

←Older revision		Revision as of 07:41, 21 August 2019
Line 1:		Line 1:

	==LC3 FUNDC1 complex structure==		==LC3 FUNDC1 complex structure==
-	<StructureSection load='2n9x' size='340' side='right' caption='[[2n9x]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2n9x' size='340' side='right'caption='[[2n9x]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2n9x]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N9X FirstGlance]. <br>		<table><tr><td colspan='2'>[[2n9x]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N9X FirstGlance]. <br>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Kuang, Y]]		[[Category: Kuang, Y]]
	[[Category: Xia, B]]		[[Category: Xia, B]]
	[[Category: Protein binding-peptide complex]]		[[Category: Protein binding-peptide complex]]
	[[Category: Protein/peptide]]		[[Category: Protein/peptide]]

OCA at 11:36, 3 August 2017

OCA — Thu, 03 Aug 2017 11:36:07 GMT

←Older revision		Revision as of 11:36, 3 August 2017
Line 8:		Line 8:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes). [[http://www.uniprot.org/uniprot/FUND1_HUMAN FUND1_HUMAN]] Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.<ref>PMID:22267086</ref>		[[http://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes). [[http://www.uniprot.org/uniprot/FUND1_HUMAN FUND1_HUMAN]] Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.<ref>PMID:22267086</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mitophagy is a fundamental process that determines mitochondrial quality and homeostasis. Several mitophagy receptors, including the newly identified FUNDC1, mediate selective removal of damaged or superfluous mitochondria through their specific interaction with LC3. However, the precise mechanism by which this interaction is regulated to initiate mitophagy is not understood. Here, we report the solution structure of LC3 in complex with a peptide containing the FUNDC1 LC3-interacting region (LIR) motif. The structure reveals a noncanonical LC3-LIR binding conformation, in which the third LIR residue (Val20) is also inserted into the hydrophobic pocket of LC3, together with the conserved residues Tyr18 and Leu21. This enables Tyr18 to be positioned near Asp19 of LC3, and thus phosphorylation of Tyr18 significantly weakens the binding affinity due to electrostatic repulsion. Functional analysis revealed that mitochondrial targeting of the LIR-containing cytosolic portion of FUNDC1 is necessary and sufficient to initiate mitophagy when Tyr18 is unphosphorylated, even in the absence of mitochondrial fragmentation. Thus, we demonstrated that phosphorylation of Tyr18 of FUNDC1 serves as a molecular switch for mitophagy. This may represent a novel target for therapeutic intervention.
		+
		+	Structural basis for the phosphorylation of FUNDC1 LIR as a molecular switch of mitophagy.,Kuang Y, Ma K, Zhou C, Ding P, Zhu Y, Chen Q, Xia B Autophagy. 2016 Dec;12(12):2363-2373. Epub 2016 Sep 21. PMID:27653272<ref>PMID:27653272</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 2n9x" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:12, 15 December 2016

OCA — Thu, 15 Dec 2016 19:12:13 GMT

←Older revision		Revision as of 19:12, 15 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 2n9x ~~is ON HOLD until Paper Publication~~	+	==LC3 FUNDC1 complex structure==
-		+	<StructureSection load='2n9x' size='340' side='right' caption='[[2n9x]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~Authors~~: ~~Xia~~, B., ~~Kuang~~, Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[2n9x]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N9X FirstGlance]. <br>
-	~~Description: LC3 FUNDC1 complex structure~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n9x OCA], [http://pdbe.org/2n9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n9x RCSB], [http://www.ebi.ac.uk/pdbsum/2n9x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n9x ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes). [[http://www.uniprot.org/uniprot/FUND1_HUMAN FUND1_HUMAN]] Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.<ref>PMID:22267086</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Kuang, Y]]		[[Category: Kuang, Y]]
	[[Category: Xia, B]]		[[Category: Xia, B]]
		+	[[Category: Protein binding-peptide complex]]
		+	[[Category: Protein/peptide]]

OCA at 12:05, 30 December 2015

OCA — Wed, 30 Dec 2015 12:05:03 GMT

←Older revision		Revision as of 12:05, 30 December 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 2n9x is ON HOLD	+	The entry 2n9x is ON HOLD until Paper Publication

-	Authors: Xia, b., Kuang, y.	+	Authors: Xia, B., Kuang, Y.

	Description: LC3 FUNDC1 complex structure		Description: LC3 FUNDC1 complex structure

OCA: Protected "2n9x" [edit=sysop:move=sysop]

OCA — Wed, 23 Dec 2015 06:42:59 GMT

Protected "2n9x" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:42, 23 December 2015

OCA: New page: '''Unreleased structure''' The entry 2n9x is ON HOLD Authors: Xia, b., Kuang, y. Description: LC3 FUNDC1 complex structure Category: Unreleased Structures Category: Kuang, Y [[...

OCA — Wed, 23 Dec 2015 06:42:57 GMT

New page: '''Unreleased structure''' The entry 2n9x is ON HOLD Authors: Xia, b., Kuang, y. Description: LC3 FUNDC1 complex structure Category: Unreleased Structures Category: Kuang, Y [[...

New page

'''Unreleased structure'''

The entry 2n9x is ON HOLD

Authors: Xia, b., Kuang, y.

Description: LC3 FUNDC1 complex structure
[[Category: Unreleased Structures]]
[[Category: Kuang, Y]]
[[Category: Xia, B]]