2nm1 - Revision history

OCA at 10:16, 30 August 2023

OCA — Wed, 30 Aug 2023 10:16:51 GMT

←Older revision		Revision as of 10:16, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='2nm1' size='340' side='right'caption='[[2nm1]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='2nm1' size='340' side='right'caption='[[2nm1]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2nm1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896~~] and [https://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NM1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2nm1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NM1 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1f31\|1f31]], [[1z0h\|1z0h]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">botB ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nm1 OCA], [https://pdbe.org/2nm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nm1 RCSB], [https://www.ebi.ac.uk/pdbsum/2nm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nm1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nm1 OCA], [https://pdbe.org/2nm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nm1 RCSB], [https://www.ebi.ac.uk/pdbsum/2nm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nm1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BXB_CLOBO BXB_CLOBO]] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-\|-Phe-77' bond of synaptobrevin-2.	+	[https://www.uniprot.org/uniprot/BXB_CLOBO BXB_CLOBO] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-\|-Phe-77' bond of synaptobrevin-2.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus botulinus van ermengem 1896]]~~	+	[[Category: Clostridium botulinum]]
-	~~[[Category: Bontoxilysin]]~~	+
-	~~[[Category: Buffalo rat~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Binz, T~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: ~~Brunger, A~~ T]]	+	[[Category: Binz T]]
-	[[Category: ~~Jin, R~~]]	+	[[Category: Brunger AT]]
-	[[Category: ~~Rummel, A~~]]	+	[[Category: Jin R]]
-	[[Category: ~~Botulism]]~~	+	[[Category: Rummel A]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Neurotransmission]]~~	+
-	~~[[Category: Synaptotagmin]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 15:45, 8 June 2021

OCA — Tue, 08 Jun 2021 15:45:32 GMT

←Older revision		Revision as of 15:45, 8 June 2021
Line 1:		Line 1:

	==Structure of BoNT/B in complex with its protein receptor==		==Structure of BoNT/B in complex with its protein receptor==
-	<StructureSection load='2nm1' size='340' side='right' caption='[[2nm1]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='2nm1' size='340' side='right'caption='[[2nm1]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2nm1]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896] and [~~http~~://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2NM1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2nm1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896] and [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NM1 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f31\|1f31]], [[1z0h\|1z0h]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1f31\|1f31]], [[1z0h\|1z0h]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botB ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2nm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nm1 OCA], [~~http~~://pdbe.org/2nm1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2nm1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2nm1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2nm1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nm1 OCA], [https://pdbe.org/2nm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nm1 RCSB], [https://www.ebi.ac.uk/pdbsum/2nm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nm1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BXB_CLOBO BXB_CLOBO]] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-\|-Phe-77' bond of synaptobrevin-2.	+	[[https://www.uniprot.org/uniprot/BXB_CLOBO BXB_CLOBO]] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-\|-Phe-77' bond of synaptobrevin-2.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]	+	*[[Botulinum neurotoxin 3D structures\|Botulinum neurotoxin 3D structures]]
		+	*[[Synaptotagmin 3D structures\|Synaptotagmin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 40:		Line 41:
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
		+	[[Category: Large Structures]]
	[[Category: Binz, T]]		[[Category: Binz, T]]
	[[Category: Brunger, A T]]		[[Category: Brunger, A T]]

OCA at 17:26, 15 August 2018

OCA — Wed, 15 Aug 2018 17:26:14 GMT

←Older revision		Revision as of 17:26, 15 August 2018
Line 1:		Line 1:
		+
	==Structure of BoNT/B in complex with its protein receptor==		==Structure of BoNT/B in complex with its protein receptor==
	<StructureSection load='2nm1' size='340' side='right' caption='[[2nm1]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='2nm1' size='340' side='right' caption='[[2nm1]], [[Resolution\|resolution]] 2.15Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nm1 OCA], [http://pdbe.org/2nm1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2nm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2nm1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nm1 OCA], [http://pdbe.org/2nm1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2nm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2nm1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2nm1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nm/2nm1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nm/2nm1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 23:30, 7 February 2016

OCA — Sun, 07 Feb 2016 23:30:55 GMT

←Older revision		Revision as of 23:30, 7 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nm1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:51, 10 September 2015

OCA — Thu, 10 Sep 2015 05:51:21 GMT

←Older revision		Revision as of 05:51, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2nm1' size='340' side='right' caption='[[2nm1]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='2nm1' size='340' side='right' caption='[[2nm1]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2nm1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_botulinum Clostridium botulinum~~] and [http://en.wikipedia.org/wiki/~~Rattus_norvegicus Rattus norvegicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NM1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2nm1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896] and [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NM1 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f31\|1f31]], [[1z0h\|1z0h]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f31\|1f31]], [[1z0h\|1z0h]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 ~~Clostridium botulinum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nm1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2nm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2nm1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nm1 OCA], [http://pdbe.org/2nm1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2nm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2nm1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2nm1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
-	[[Category: ~~Clostridium botulinum]]~~	+	[[Category: Buffalo rat]]
-	~~[[Category: Rattus norvegicus~~]]	+
	[[Category: Binz, T]]		[[Category: Binz, T]]
	[[Category: Brunger, A T]]		[[Category: Brunger, A T]]

OCA at 18:58, 24 December 2014

OCA — Wed, 24 Dec 2014 18:58:00 GMT

←Older revision		Revision as of 18:58, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2nm1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NM1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2nm1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NM1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f31\|1f31]], [[1z0h\|1z0h]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f31\|1f31]], [[1z0h\|1z0h]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nm1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2nm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2nm1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nm1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2nm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2nm1 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BXB_CLOBO BXB_CLOBO]] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-\|-Phe-77' bond of synaptobrevin-2.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
-	[[Category: Binz, T.]]	+	[[Category: Binz, T]]
-	[[Category: Brunger, A T.]]	+	[[Category: Brunger, A T]]
-	[[Category: Jin, R.]]	+	[[Category: Jin, R]]
-	[[Category: Rummel, A.]]	+	[[Category: Rummel, A]]
	[[Category: Botulism]]		[[Category: Botulism]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 19:35, 30 September 2014

OCA — Tue, 30 Sep 2014 19:35:29 GMT

←Older revision		Revision as of 19:35, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2nm1.~~png~~\|~~left~~\|200px]]	+	==Structure of BoNT/B in complex with its protein receptor==
		+	<StructureSection load='2nm1' size='340' side='right' caption='[[2nm1]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2nm1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NM1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f31\|1f31]], [[1z0h\|1z0h]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">botB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 Clostridium botulinum])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nm1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2nm1 RCSB], [http://www.ebi.ac.uk/pdbsum/2nm1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nm/2nm1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Botulinum neurotoxins (BoNTs) are produced by Clostridium botulinum and cause the neuroparalytic syndrome of botulism. With a lethal dose of 1 ng kg(-1), they pose a biological hazard to humans and a serious potential bioweapon threat. BoNTs bind with high specificity at neuromuscular junctions and they impair exocytosis of synaptic vesicles containing acetylcholine through specific proteolysis of SNAREs (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors), which constitute part of the synaptic vesicle fusion machinery. The molecular details of the toxin-cell recognition have been elusive. Here we report the structure of a BoNT in complex with its protein receptor: the receptor-binding domain of botulinum neurotoxin serotype B (BoNT/B) bound to the luminal domain of synaptotagmin II, determined at 2.15 A resolution. On binding, a helix is induced in the luminal domain which binds to a saddle-shaped crevice on a distal tip of BoNT/B. This crevice is adjacent to the non-overlapping ganglioside-binding site of BoNT/B. Synaptotagmin II interacts with BoNT/B with nanomolar affinity, at both neutral and acidic endosomal pH. Biochemical and neuronal ex vivo studies of structure-based mutations indicate high specificity and affinity of the interaction, and high selectivity of BoNT/B among synaptotagmin I and II isoforms. Synergistic binding of both synaptotagmin and ganglioside imposes geometric restrictions on the initiation of BoNT/B translocation after endocytosis. Our results provide the basis for the rational development of preventive vaccines or inhibitors against these neurotoxins.

-	~~{{STRUCTURE_2nm1\| PDB=2nm1 \| SCENE= }}~~	+	Botulinum neurotoxin B recognizes its protein receptor with high affinity and specificity.,Jin R, Rummel A, Binz T, Brunger AT Nature. 2006 Dec 21;444(7122):1092-5. Epub 2006 Dec 13. PMID:17167421<ref>PMID:17167421</ref>

-	~~===Structure of BoNT~~/~~B in complex with its protein receptor===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17167421}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2nm1]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_botulinum Clostridium botulinum] and [http://en~~.~~wikipedia.org~~/~~wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM1 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017167421<~~/~~ref~~><~~ref group="xtra">PMID:010932256</ref><ref group="xtra">PMID:015781237</ref><references group="xtra"~~/>	+
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]

OCA at 13:10, 30 January 2013

OCA — Wed, 30 Jan 2013 13:10:03 GMT

←Older revision		Revision as of 13:10, 30 January 2013
Line 1:		Line 1:
	[[Image:2nm1.png\|left\|200px]]		[[Image:2nm1.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2nm1", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2nm1\| PDB=2nm1 \| SCENE= }}		{{STRUCTURE_2nm1\| PDB=2nm1 \| SCENE= }}

	===Structure of BoNT/B in complex with its protein receptor===		===Structure of BoNT/B in complex with its protein receptor===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17167421}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17167421 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17167421}}		{{ABSTRACT_PUBMED_17167421}}

Line 22:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17167421~~</ref><ref group="xtra">PMID:~~10932256~~</ref><ref group="xtra">PMID:~~15781237~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017167421</ref><ref group="xtra">PMID:010932256</ref><ref group="xtra">PMID:015781237</ref><references group="xtra"/>
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]

OCA: Protected "2nm1" [edit=sysop:move=sysop]

OCA — Mon, 14 Mar 2011 22:36:44 GMT

Protected "2nm1" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:36, 14 March 2011

OCA at 22:36, 14 March 2011

OCA — Mon, 14 Mar 2011 22:36:43 GMT

←Older revision		Revision as of 22:36, 14 March 2011
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2nm1.png\|left\|200px]]		[[Image:2nm1.png\|left\|200px]]

Line 20:		Line 19:

	==About this Structure==		==About this Structure==
-	~~2NM1~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM1 OCA].	+	[[2nm1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NM1 OCA].

	==Reference==		==Reference==
Line 32:		Line 31:
	[[Category: Rummel, A.]]		[[Category: Rummel, A.]]
	[[Category: Botulism]]		[[Category: Botulism]]
		+	[[Category: Hydrolase]]
	[[Category: Neurotransmission]]		[[Category: Neurotransmission]]
	[[Category: Synaptotagmin]]		[[Category: Synaptotagmin]]
-		+	[[Category: Toxin]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 17:12:14 2009''~~	+