2nzg - Revision history

OCA: Redirecting to 3efx

OCA — Wed, 24 Sep 2008 07:28:31 GMT

Redirecting to 3efx

←Older revision		Revision as of 07:28, 24 September 2008
Line 1:		Line 1:
-	~~{{Seed}}~~	+	#REDIRECT [[3efx]] This PDB entry is obsolete and replaced by 3efx
-	[[~~Image:2nzg.png\|left\|200px~~]]	+
-		+
-	~~<!--~~	+
-	~~The line below this paragraph, containing "STRUCTURE_2nzg", creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	~~-->~~	+
-	~~{{STRUCTURE_2nzg\| PDB=2nzg \| SCENE= }}~~	+
-		+
-	~~===Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details===~~	+
-		+
-		+
-	~~<!--~~	+
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_15341730}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 15341730 is the PubMed ID number.~~	+
-	~~-->~~	+
-	~~{{ABSTRACT_PUBMED_15341730}}~~	+
-		+
-	~~==About this Structure==~~	+
-	This ~~structure supersedes the now removed~~ PDB entry ~~[http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information~~ is ~~available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZG OCA].~~	+
-		+
-	~~==Reference==~~	+
-	~~Novel binding site identified in a hybrid between cholera toxin~~ and heat-labile enterotoxin: 1.9 A crystal structure reveals the details., Holmner A, Lebens M, Teneberg S, Angstrom J, Okvist M, Krengel U, Structure. 2004 Sep;12(9):1655-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341730 15341730]	+
-	~~[[Category: Angstrom, J.]]~~	+
-	~~[[Category: Holmner, A.]]~~	+
-	~~[[Category: Krengel, U.]]~~	+
-	~~[[Category: Lebens, M.]]~~	+
-	~~[[Category: Okvist, M.]]~~	+
-	~~[[Category: Teneberg, S.]]~~	+
-	~~[[Category: Blood group antigen]]~~	+
-	~~[[Category: Cholera toxin]]~~	+
-	~~[[Category: Heat-labile enterotoxin]]~~	+
-	~~[[Category: Protein-carbohydrate complex]]~~	+
-		+
-	~~''Page seeded~~ by ~~[http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:22:43 2008''~~	+

OCA at 03:22, 29 July 2008

OCA — Tue, 29 Jul 2008 03:22:45 GMT

←Older revision		Revision as of 03:22, 29 July 2008
Line 1:		Line 1:
-	[[Image:2nzg.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2nzg.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2nzg\| PDB=2nzg \| SCENE= }}		{{STRUCTURE_2nzg\| PDB=2nzg \| SCENE= }}

-	~~'''~~Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details~~'''~~	+	===Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details===


-	~~==Overview==~~	+	<!--
-	A hybrid between the B subunits of cholera toxin and Escherichia coli heat-labile enterotoxin has been described, which exhibits a novel binding specificity to blood group A and B type 2 determinants. In the present investigation, we have determined the crystal structure of this ~~protein hybrid~~, ~~termed LCTBK~~, ~~in complex with~~ the ~~blood group A pentasaccharide GalNAcalpha3(Fucalpha2)Galbeta4(Fucalpha3)GlcNAcbeta, confirming not only~~ the ~~novel binding specificity but also a distinct new oligosaccharide binding site. Binding studies revealed that the new specificity can be ascribed to a single mutation~~ (~~S4N) introduced into the sequence of Escherichia coli heat-labile enterotoxin~~. ~~At a resolution of 1~~.~~9 A~~, ~~the new binding site is resolved in excellent detail. Main features include a complex network of water molecules, which~~ is ~~well preserved by~~ the ~~parent toxins, and an unexpectedly modest contribution to binding by the critical residue Asn4, which interacts with the ligand only via a single water molecule~~.	+	The line below this paragraph, {{ABSTRACT_PUBMED_15341730}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15341730 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15341730}}

	==About this Structure==		==About this Structure==
Line 30:		Line 34:
	[[Category: Heat-labile enterotoxin]]		[[Category: Heat-labile enterotoxin]]
	[[Category: Protein-carbohydrate complex]]		[[Category: Protein-carbohydrate complex]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 10:~~06:59 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:22:43 2008''

OCA at 07:06, 4 May 2008

OCA — Sun, 04 May 2008 07:06:59 GMT

←Older revision		Revision as of 07:06, 4 May 2008
Line 1:		Line 1:
	[[Image:2nzg.gif\|left\|200px]]		[[Image:2nzg.gif\|left\|200px]]

-	{{Structure	+	<!--
-	\|PDB~~= 2nzg \|SIZE=350\|CAPTION= <~~scene ~~name='initialview01'>2nzg</scene>~~, ~~resolution 1~~.~~94Å~~	+	The line below this paragraph, containing "STRUCTURE_2nzg", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <scene name='pdbligand=FUC:ALPHA~~-L-~~FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene~~>	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	\|~~ACTIVITY~~=	+	or leave the SCENE parameter empty for the default display.
-	\|~~GENE~~=	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_2nzg\| PDB=2nzg \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzg OCA], [http://www.ebi.ac.uk/pdbsum/2nzg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nzg RCSB]</span>	+
-	}}	+

	'''Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details'''		'''Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details'''
Line 19:		Line 16:

	==About this Structure==		==About this Structure==
-	~~2NZG is a [[Protein complex]]~~ structure ~~of sequences from~~ [http://en.~~wikipedia~~.~~org~~/~~wiki~~/~~Vibrio_cholerae Vibrio cholerae~~]~~. This structure supersedes the now removed PDB entry 1TL0~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZG OCA].	+	This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1tl0 1tl0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZG OCA].

	==Reference==		==Reference==
	Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin: 1.9 A crystal structure reveals the details., Holmner A, Lebens M, Teneberg S, Angstrom J, Okvist M, Krengel U, Structure. 2004 Sep;12(9):1655-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341730 15341730]		Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin: 1.9 A crystal structure reveals the details., Holmner A, Lebens M, Teneberg S, Angstrom J, Okvist M, Krengel U, Structure. 2004 Sep;12(9):1655-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341730 15341730]
-	[[Category: Protein complex]]
-	[[Category: Vibrio cholerae]]
	[[Category: Angstrom, J.]]		[[Category: Angstrom, J.]]
	[[Category: Holmner, A.]]		[[Category: Holmner, A.]]
Line 31:		Line 26:
	[[Category: Okvist, M.]]		[[Category: Okvist, M.]]
	[[Category: Teneberg, S.]]		[[Category: Teneberg, S.]]
-	[[Category: ~~blood~~ group antigen]]	+	[[Category: Blood group antigen]]
-	[[Category: ~~cholera~~ toxin]]	+	[[Category: Cholera toxin]]
-	[[Category: ~~heat~~-labile enterotoxin]]	+	[[Category: Heat-labile enterotoxin]]
-	[[Category: ~~protein~~-carbohydrate complex]]	+	[[Category: Protein-carbohydrate complex]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:06:59 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 04:~~10:36 2008''	+

OCA at 01:10, 31 March 2008

OCA — Mon, 31 Mar 2008 01:10:37 GMT

←Older revision		Revision as of 01:10, 31 March 2008
Line 4:		Line 4:
	\|PDB= 2nzg \|SIZE=350\|CAPTION= <scene name='initialview01'>2nzg</scene>, resolution 1.94Å		\|PDB= 2nzg \|SIZE=350\|CAPTION= <scene name='initialview01'>2nzg</scene>, resolution 1.94Å
	\|SITE=		\|SITE=
-	\|LIGAND=	+	\|LIGAND= <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>
	\|ACTIVITY=		\|ACTIVITY=
	\|GENE=		\|GENE=
		+	\|DOMAIN=
		+	\|RELATEDENTRY=
		+	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzg OCA], [http://www.ebi.ac.uk/pdbsum/2nzg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nzg RCSB]</span>
	}}		}}

Line 33:		Line 36:
	[[Category: protein-carbohydrate complex]]		[[Category: protein-carbohydrate complex]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Mar ~~20 17:53~~:10 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:10:36 2008''

OCA at 15:53, 20 March 2008

OCA — Thu, 20 Mar 2008 15:53:12 GMT

←Older revision		Revision as of 15:53, 20 March 2008
Line 1:		Line 1:
-	[[Image:2nzg.gif\|left\|200px]]~~<br /><applet load~~="2nzg~~" size~~="350~~" color~~=~~"white" frame~~=~~"true" align="right" spinBox="true"~~	+	[[Image:2nzg.gif\|left\|200px]]
-	~~caption="~~2nzg, resolution 1.94Å~~" />~~	+
-	'''Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details'''~~<br />~~	+	{{Structure
		+	\|PDB= 2nzg \|SIZE=350\|CAPTION= <scene name='initialview01'>2nzg</scene>, resolution 1.94Å
		+	\|SITE=
		+	\|LIGAND=
		+	\|ACTIVITY=
		+	\|GENE=
		+	}}
		+
		+	'''Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details'''
		+

	==Overview==		==Overview==
Line 7:		Line 16:

	==About this Structure==		==About this Structure==
-	2NZG is a [~~http://en.wikipedia.org/wiki/Protein_complex~~ Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. This structure supersedes the now removed PDB entry 1TL0. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZG OCA].	+	2NZG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. This structure supersedes the now removed PDB entry 1TL0. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZG OCA].

	==Reference==		==Reference==
-	Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin: 1.9 A crystal structure reveals the details., Holmner A, Lebens M, Teneberg S, Angstrom J, Okvist M, Krengel U, Structure. 2004 Sep;12(9):1655-67. PMID:[http://~~ispc~~.~~weizmann~~.ac.~~il/~~/~~pmbin~~/~~getpm?pmid=~~15341730 15341730]	+	Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin: 1.9 A crystal structure reveals the details., Holmner A, Lebens M, Teneberg S, Angstrom J, Okvist M, Krengel U, Structure. 2004 Sep;12(9):1655-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341730 15341730]
	[[Category: Protein complex]]		[[Category: Protein complex]]
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
Line 24:		Line 33:
	[[Category: protein-carbohydrate complex]]		[[Category: protein-carbohydrate complex]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu ~~Feb 21 18~~:12:49 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:53:10 2008''

OCA at 16:12, 21 February 2008

OCA — Thu, 21 Feb 2008 16:12:50 GMT

←Older revision		Revision as of 16:12, 21 February 2008
Line 1:		Line 1:
-	[[Image:2nzg.gif\|left\|200px]]<br /><applet load="2nzg" size="~~450~~" color="white" frame="true" align="right" spinBox="true"	+	[[Image:2nzg.gif\|left\|200px]]<br /><applet load="2nzg" size="350" color="white" frame="true" align="right" spinBox="true"
	caption="2nzg, resolution 1.94Å" />		caption="2nzg, resolution 1.94Å" />
	'''Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details'''<br />		'''Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details'''<br />

	==Overview==		==Overview==
-	A hybrid between the B subunits of cholera toxin and Escherichia coli, heat-labile enterotoxin has been described, which exhibits a novel binding, specificity to blood group A and B type 2 determinants. In the present, investigation, we have determined the crystal structure of this protein, hybrid, termed LCTBK, in complex with the blood group A pentasaccharide, GalNAcalpha3(Fucalpha2)Galbeta4(Fucalpha3)GlcNAcbeta, confirming not only, the novel binding specificity but also a distinct new oligosaccharide, binding site. Binding studies revealed that the new specificity can be, ascribed to a single mutation (S4N) introduced into the sequence of, Escherichia coli heat-labile enterotoxin. At a resolution of 1.9 A, the, new binding site is resolved in excellent detail. Main features include a, complex network of water molecules, which is well preserved by the parent, toxins, and an unexpectedly modest contribution to binding by the critical, residue Asn4, which interacts with the ligand only via a single water, molecule.	+	A hybrid between the B subunits of cholera toxin and Escherichia coli heat-labile enterotoxin has been described, which exhibits a novel binding specificity to blood group A and B type 2 determinants. In the present investigation, we have determined the crystal structure of this protein hybrid, termed LCTBK, in complex with the blood group A pentasaccharide GalNAcalpha3(Fucalpha2)Galbeta4(Fucalpha3)GlcNAcbeta, confirming not only the novel binding specificity but also a distinct new oligosaccharide binding site. Binding studies revealed that the new specificity can be ascribed to a single mutation (S4N) introduced into the sequence of Escherichia coli heat-labile enterotoxin. At a resolution of 1.9 A, the new binding site is resolved in excellent detail. Main features include a complex network of water molecules, which is well preserved by the parent toxins, and an unexpectedly modest contribution to binding by the critical residue Asn4, which interacts with the ligand only via a single water molecule.

	==About this Structure==		==About this Structure==
-	2NZG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. This structure ~~superseeds~~ the now removed PDB entry 1TL0. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2NZG OCA].	+	2NZG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. This structure supersedes the now removed PDB entry 1TL0. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZG OCA].

	==Reference==		==Reference==
Line 24:		Line 24:
	[[Category: protein-carbohydrate complex]]		[[Category: protein-carbohydrate complex]]

-	''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 12:59:~~55 2007~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:12:49 2008''

OCA: New page: 200px
'''Novel binding site i...
OCA — Wed, 21 Nov 2007 10:52:39 GMT

New page: 200px<br /><applet load="2nzg" size="450" color="white" frame="true" align="right" spinBox="true" caption="2nzg, resolution 1.94Å" /> '''Novel binding site i...

New page
[[Image:2nzg.gif|left|200px]]<br /><applet load="2nzg" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2nzg, resolution 1.94Å" />
'''Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin, 1.9A crystal structure reveals the details'''<br />

==Overview==
A hybrid between the B subunits of cholera toxin and Escherichia coli, heat-labile enterotoxin has been described, which exhibits a novel binding, specificity to blood group A and B type 2 determinants. In the present, investigation, we have determined the crystal structure of this protein, hybrid, termed LCTBK, in complex with the blood group A pentasaccharide, GalNAcalpha3(Fucalpha2)Galbeta4(Fucalpha3)GlcNAcbeta, confirming not only, the novel binding specificity but also a distinct new oligosaccharide, binding site. Binding studies revealed that the new specificity can be, ascribed to a single mutation (S4N) introduced into the sequence of, Escherichia coli heat-labile enterotoxin. At a resolution of 1.9 A, the, new binding site is resolved in excellent detail. Main features include a, complex network of water molecules, which is well preserved by the parent, toxins, and an unexpectedly modest contribution to binding by the critical, residue Asn4, which interacts with the ligand only via a single water, molecule.

==About this Structure==
2NZG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. This structure superseeds the now removed PDB entry 1TL0. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2NZG OCA].

==Reference==
Novel binding site identified in a hybrid between cholera toxin and heat-labile enterotoxin: 1.9 A crystal structure reveals the details., Holmner A, Lebens M, Teneberg S, Angstrom J, Okvist M, Krengel U, Structure. 2004 Sep;12(9):1655-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15341730 15341730]
[[Category: Protein complex]]
[[Category: Vibrio cholerae]]
[[Category: Angstrom, J.]]
[[Category: Holmner, A.]]
[[Category: Krengel, U.]]
[[Category: Lebens, M.]]
[[Category: Okvist, M.]]
[[Category: Teneberg, S.]]
[[Category: blood group antigen]]
[[Category: cholera toxin]]
[[Category: heat-labile enterotoxin]]
[[Category: protein-carbohydrate complex]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:59:55 2007''