2nzz - Revision history

OCA at 12:52, 20 December 2023

OCA — Wed, 20 Dec 2023 12:52:45 GMT

←Older revision		Revision as of 12:52, 20 December 2023
Line 1:		Line 1:

	==NMR structure analysis of the Penetratin conjugated Gas (374-394) peptide==		==NMR structure analysis of the Penetratin conjugated Gas (374-394) peptide==
-	<StructureSection load='2nzz' size='340' side='right'caption='[[2nzz~~]], [[NMR_Ensembles_of_Models \| 30 NMR models~~]]' scene=''>	+	<StructureSection load='2nzz' size='340' side='right'caption='[[2nzz]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2nzz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NZZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2nzz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NZZ FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[2o00\|2o00]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzz OCA], [https://pdbe.org/2nzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nzz RCSB], [https://www.ebi.ac.uk/pdbsum/2nzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nzz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzz OCA], [https://pdbe.org/2nzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nzz RCSB], [https://www.ebi.ac.uk/pdbsum/2nzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nzz ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Albrizio, S]]	+	[[Category: Albrizio S]]
-	[[Category: Errico, G ~~D]]~~	+	[[Category: D'Errico G]]
-	~~[[Category: Espsito, C]]~~	+	[[Category: D'Ursi AM]]
-	~~[[Category: Novellino, E]]~~	+	[[Category: Espsito C]]
-	~~[[Category: Rovero, P~~]]	+	[[Category: Novellino E]]
-	[[Category: Ursi~~, A M.D~~]]	+	[[Category: Rovero P]]
-	[[Category: ~~A2a adenosine receptor~~]]	+
-	[[Category: ~~Cell-penetrating peptide~~]]	+
-	[[Category: ~~Conformational analysis]]~~	+
-	~~[[Category: G protein]]~~	+
-	~~[[Category: Gas subunit]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Penetratin~~]]	+

OCA at 09:08, 23 February 2022

OCA — Wed, 23 Feb 2022 09:08:22 GMT

←Older revision		Revision as of 09:08, 23 February 2022
Line 3:		Line 3:
	<StructureSection load='2nzz' size='340' side='right'caption='[[2nzz]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>		<StructureSection load='2nzz' size='340' side='right'caption='[[2nzz]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2nzz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2NZZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2nzz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NZZ FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o00\|2o00]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2o00\|2o00]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2nzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzz OCA], [~~http~~://pdbe.org/2nzz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2nzz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2nzz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2nzz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzz OCA], [https://pdbe.org/2nzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nzz RCSB], [https://www.ebi.ac.uk/pdbsum/2nzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nzz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:36, 20 May 2020

OCA — Wed, 20 May 2020 06:36:45 GMT

←Older revision		Revision as of 06:36, 20 May 2020
Line 1:		Line 1:

	==NMR structure analysis of the Penetratin conjugated Gas (374-394) peptide==		==NMR structure analysis of the Penetratin conjugated Gas (374-394) peptide==
-	<StructureSection load='2nzz' size='340' side='right' caption='[[2nzz]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>	+	<StructureSection load='2nzz' size='340' side='right'caption='[[2nzz]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2nzz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2NZZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2nzz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2NZZ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o00\|2o00]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o00\|2o00]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2nzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzz OCA], [http://pdbe.org/2nzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2nzz RCSB], [http://www.ebi.ac.uk/pdbsum/2nzz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2nzz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2nzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzz OCA], [http://pdbe.org/2nzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2nzz RCSB], [http://www.ebi.ac.uk/pdbsum/2nzz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2nzz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Albrizio, S]]		[[Category: Albrizio, S]]
	[[Category: Errico, G D]]		[[Category: Errico, G D]]

OCA at 17:11, 15 August 2018

OCA — Wed, 15 Aug 2018 17:11:24 GMT

←Older revision		Revision as of 17:11, 15 August 2018
Line 1:		Line 1:
		+
	==NMR structure analysis of the Penetratin conjugated Gas (374-394) peptide==		==NMR structure analysis of the Penetratin conjugated Gas (374-394) peptide==
	<StructureSection load='2nzz' size='340' side='right' caption='[[2nzz]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>		<StructureSection load='2nzz' size='340' side='right' caption='[[2nzz]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2nzz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NZZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2nzz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NZZ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o00\|2o00]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o00\|2o00]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzz OCA], [http://pdbe.org/2nzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2nzz RCSB], [http://www.ebi.ac.uk/pdbsum/2nzz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzz OCA], [http://pdbe.org/2nzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2nzz RCSB], [http://www.ebi.ac.uk/pdbsum/2nzz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2nzz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:16, 11 September 2015

OCA — Fri, 11 Sep 2015 03:16:18 GMT

←Older revision		Revision as of 03:16, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[2nzz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NZZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2nzz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NZZ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o00\|2o00]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o00\|2o00]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2nzz RCSB], [http://www.ebi.ac.uk/pdbsum/2nzz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzz OCA], [http://pdbe.org/2nzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2nzz RCSB], [http://www.ebi.ac.uk/pdbsum/2nzz PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2nzz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:50, 22 January 2015

OCA — Thu, 22 Jan 2015 06:50:20 GMT

←Older revision		Revision as of 06:50, 22 January 2015
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Albrizio, S.]]	+	[[Category: Albrizio, S]]
-	[[Category: Errico, G D.]]	+	[[Category: Errico, G D]]
-	[[Category: Espsito, C.]]	+	[[Category: Espsito, C]]
-	[[Category: Novellino, E.]]	+	[[Category: Novellino, E]]
-	[[Category: Rovero, P.]]	+	[[Category: Rovero, P]]
-	[[Category: Ursi, A M.D.]]	+	[[Category: Ursi, A M.D]]
	[[Category: A2a adenosine receptor]]		[[Category: A2a adenosine receptor]]
	[[Category: Cell-penetrating peptide]]		[[Category: Cell-penetrating peptide]]

OCA at 11:34, 20 October 2014

OCA — Mon, 20 Oct 2014 11:34:19 GMT

←Older revision		Revision as of 11:34, 20 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2nzz.~~png~~\|~~left~~\|~~200px~~]]	+	==NMR structure analysis of the Penetratin conjugated Gas (374-394) peptide==
		+	<StructureSection load='2nzz' size='340' side='right' caption='[[2nzz]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2nzz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NZZ FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o00\|2o00]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nzz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2nzz RCSB], [http://www.ebi.ac.uk/pdbsum/2nzz PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A42 is a chimera peptide consisting of Galphas(374-394)C379A--the 21-mer C terminus of the Galphas protein, able of adenosine inhibitory activity--and penetratin--the 16 residue fragment, derived from the homeodomain of the Drosophila transcription factor Antennapedia. A42 is able to cross cell membranes and to inhibit A2A and A2B adenosine and beta-adrenergic receptor stimulated camps (D'Ursi et al. Mol. Pharmacol. 2006, 69, 727-36). Here we present an extensive biophysical study of A42 in different membrane mimetics, with the objective to evaluate the molecular mechanisms which promote the membrane permeation. Fluorescence, CD, and NMR data were acquired in the presence of negatively charged and zwitterionic sodium dodecyl sulfate and dodecylphosphocholine surfactants. To validate the spectroscopic results in a larger scale, fluorescence microscopy experiments were performed on negatively charged and zwitterionic dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylcholine vesicles. Our results show that the internalization of A42 is mainly driven by electrostatic interactions, hydrophobic interactions playing only a secondary, sinergistic role. The distribution of the charges along the molecule has an important role, highlighting that internalization is a process which requires a specific matching of peptide and membrane properties.

-	~~{{STRUCTURE_2nzz\| PDB=2nzz \| SCENE= }}~~	+	Driving forces in the delivery of penetratin conjugated G protein fragment.,Albrizio S, Giusti L, D'Errico G, Esposito C, Porchia F, Caliendo G, Novellino E, Mazzoni MR, Rovero P, D'Ursi AM J Med Chem. 2007 Apr 5;50(7):1458-64. Epub 2007 Mar 10. PMID:17348636<ref>PMID:17348636</ref>

-	~~===NMR structure analysis~~ of the ~~Penetratin conjugated Gas (374-394) peptide===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17348636}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2nzz]] is a 1 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2NZZ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017348636<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Albrizio, S.]]		[[Category: Albrizio, S.]]
	[[Category: Errico, G D.]]		[[Category: Errico, G D.]]

OCA: Protected "2nzz" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 14:25:03 GMT

Protected "2nzz" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:25, 7 January 2013

OCA at 14:25, 7 January 2013

OCA — Mon, 07 Jan 2013 14:25:00 GMT

←Older revision		Revision as of 14:25, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2nzz.png\|left\|200px]]		[[Image:2nzz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2nzz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2nzz\| PDB=2nzz \| SCENE= }}		{{STRUCTURE_2nzz\| PDB=2nzz \| SCENE= }}

	===NMR structure analysis of the Penetratin conjugated Gas (374-394) peptide===		===NMR structure analysis of the Penetratin conjugated Gas (374-394) peptide===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17348636}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17348636 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17348636}}		{{ABSTRACT_PUBMED_17348636}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA].	+	[[2nzz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA].

	==Reference==		==Reference==
-	Driving forces in the delivery of penetratin conjugated G protein fragment., Albrizio S, Giusti L, D'Errico G, Esposito C, Porchia F, Caliendo G, Novellino E, Mazzoni MR, Rovero P, D'Ursi AM, J Med Chem. 2007 Apr 5;50(7):1458-64. Epub 2007 Mar 10. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~17348636 17348636]~~	+	<ref group="xtra">PMID:017348636</ref><references group="xtra"/>
	[[Category: Albrizio, S.]]		[[Category: Albrizio, S.]]
	[[Category: Errico, G D.]]		[[Category: Errico, G D.]]
Line 35:		Line 23:
	[[Category: G protein]]		[[Category: G protein]]
	[[Category: Gas subunit]]		[[Category: Gas subunit]]
-	[[Category: ~~Nmr~~]]	+	[[Category: Membrane protein]]
	[[Category: Penetratin]]		[[Category: Penetratin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:58:02 2008''

OCA at 15:58, 28 July 2008

OCA — Mon, 28 Jul 2008 15:58:09 GMT

←Older revision		Revision as of 15:58, 28 July 2008
Line 1:		Line 1:
-	[[Image:2nzz.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2nzz.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2nzz\| PDB=2nzz \| SCENE= }}		{{STRUCTURE_2nzz\| PDB=2nzz \| SCENE= }}

-	~~'''~~NMR structure analysis of the Penetratin conjugated Gas (374-394) peptide~~'''~~	+	===NMR structure analysis of the Penetratin conjugated Gas (374-394) peptide===


-	~~==Overview==~~	+	<!--
-	~~A42 is a chimera peptide consisting of Galphas(374~~-~~394)C379A~~-~~-the 21-mer C terminus of the Galphas protein~~, ~~able of adenosine inhibitory activity--and penetratin--the 16 residue fragment~~, ~~derived from~~ the ~~homeodomain of~~ the ~~Drosophila transcription factor Antennapedia. A42 is able to cross cell membranes and to inhibit A2A and A2B adenosine and beta-adrenergic receptor stimulated camps~~ (~~D'Ursi et al~~. ~~Mol~~. ~~Pharmacol. 2006, 69, 727-36~~)~~. Here we present an extensive biophysical study of A42 in different membrane mimetics~~, with the objective to evaluate the molecular mechanisms which promote the membrane permeation. Fluorescence, CD, and NMR data were acquired in the presence of negatively charged and zwitterionic sodium dodecyl sulfate and dodecylphosphocholine surfactants. To validate the spectroscopic results in a larger scale, fluorescence microscopy experiments were performed on negatively charged and zwitterionic dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylcholine vesicles. Our results show that the internalization of A42 is ~~mainly driven by electrostatic interactions, hydrophobic interactions playing only a secondary, sinergistic role. The distribution of~~ the ~~charges along the molecule has an important role, highlighting that internalization is a process which requires a specific matching of peptide and membrane properties~~.	+	The line below this paragraph, {{ABSTRACT_PUBMED_17348636}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17348636 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17348636}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZZ OCA].

	==Reference==		==Reference==
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	[[Category: Nmr]]		[[Category: Nmr]]
	[[Category: Penetratin]]		[[Category: Penetratin]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 10~~:07:56 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:58:02 2008''