2o5z - Revision history

OCA at 00:14, 28 December 2023

OCA — Thu, 28 Dec 2023 00:14:48 GMT

←Older revision		Revision as of 00:14, 28 December 2023
Line 3:		Line 3:
	<StructureSection load='2o5z' size='340' side='right'caption='[[2o5z]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2o5z' size='340' side='right'caption='[[2o5z]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2o5z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O5Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2o5z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O5Z FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ANO:5~~-~~BETA-ANDROSTANE-3~~,~~17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2o5x\|2o5x]]~~, ~~[[2o5y\|2o5y]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANO:5-BETA-ANDROSTANE-3,17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [https://pdbe.org/2o5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [https://www.ebi.ac.uk/pdbsum/2o5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o5z ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [https://pdbe.org/2o5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [https://www.ebi.ac.uk/pdbsum/2o5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o5z ProSAT]</span></td></tr>
	</table>		</table>
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Verdino, P]]	+	[[Category: Verdino P]]
-	[[Category: Wilson~~, I A]]~~	+	[[Category: Wilson IA]]
-	~~[[Category: Antibody engineering]]~~	+
-	~~[[Category: Chimeric fab]]~~	+
-	~~[[Category: Evolution of ligand recognition]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunoglobulin~~]]	+

OCA at 07:50, 2 March 2022

OCA — Wed, 02 Mar 2022 07:50:13 GMT

←Older revision		Revision as of 07:50, 2 March 2022
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	<StructureSection load='2o5z' size='340' side='right'caption='[[2o5z]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2o5z' size='340' side='right'caption='[[2o5z]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2o5z]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O5Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2O5Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2o5z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O5Z FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANO:5-BETA-ANDROSTANE-3,17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANO:5-BETA-ANDROSTANE-3,17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o5x\|2o5x]], [[2o5y\|2o5y]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2o5x\|2o5x]], [[2o5y\|2o5y]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [~~http~~://pdbe.org/2o5z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2o5z PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2o5z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [https://pdbe.org/2o5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [https://www.ebi.ac.uk/pdbsum/2o5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o5z ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:58, 27 May 2020

OCA — Wed, 27 May 2020 07:58:05 GMT

←Older revision		Revision as of 07:58, 27 May 2020
Line 1:		Line 1:

	==Crystal structure of the 1E9 LeuH47Trp/ArgH100Trp Fab 5-beta-androstane-3,17-dione complex==		==Crystal structure of the 1E9 LeuH47Trp/ArgH100Trp Fab 5-beta-androstane-3,17-dione complex==
-	<StructureSection load='2o5z' size='340' side='right' caption='[[2o5z]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='2o5z' size='340' side='right'caption='[[2o5z]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2o5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O5Z OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2O5Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2o5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O5Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2O5Z FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANO:5-BETA-ANDROSTANE-3,17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANO:5-BETA-ANDROSTANE-3,17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o5x\|2o5x]], [[2o5y\|2o5y]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o5x\|2o5x]], [[2o5y\|2o5y]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [http://pdbe.org/2o5z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [http://www.ebi.ac.uk/pdbsum/2o5z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2o5z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [http://pdbe.org/2o5z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [http://www.ebi.ac.uk/pdbsum/2o5z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2o5z ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Verdino, P]]		[[Category: Verdino, P]]

OCA at 07:29, 22 August 2018

OCA — Wed, 22 Aug 2018 07:29:20 GMT

←Older revision		Revision as of 07:29, 22 August 2018
Line 1:		Line 1:
		+
	==Crystal structure of the 1E9 LeuH47Trp/ArgH100Trp Fab 5-beta-androstane-3,17-dione complex==		==Crystal structure of the 1E9 LeuH47Trp/ArgH100Trp Fab 5-beta-androstane-3,17-dione complex==
	<StructureSection load='2o5z' size='340' side='right' caption='[[2o5z]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2o5z' size='340' side='right' caption='[[2o5z]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANO:5-BETA-ANDROSTANE-3,17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANO:5-BETA-ANDROSTANE-3,17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o5x\|2o5x]], [[2o5y\|2o5y]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o5x\|2o5x]], [[2o5y\|2o5y]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [http://pdbe.org/2o5z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [http://www.ebi.ac.uk/pdbsum/2o5z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [http://pdbe.org/2o5z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [http://www.ebi.ac.uk/pdbsum/2o5z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2o5z ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o5/2o5z_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o5/2o5z_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:34, 9 February 2016

OCA — Tue, 09 Feb 2016 08:34:03 GMT

←Older revision		Revision as of 08:34, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o5z ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:29, 10 September 2015

OCA — Thu, 10 Sep 2015 20:29:47 GMT

←Older revision		Revision as of 20:29, 10 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANO:5-BETA-ANDROSTANE-3,17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANO:5-BETA-ANDROSTANE-3,17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o5x\|2o5x]], [[2o5y\|2o5y]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o5x\|2o5x]], [[2o5y\|2o5y]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [http://www.ebi.ac.uk/pdbsum/2o5z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [http://pdbe.org/2o5z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [http://www.ebi.ac.uk/pdbsum/2o5z PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2o5z" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:58, 19 January 2015

OCA — Mon, 19 Jan 2015 15:58:07 GMT

←Older revision		Revision as of 15:58, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2o5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O5Z FirstGlance]. <br>		<table><tr><td colspan='2'>[[2o5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O5Z FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANO:5-BETA-ANDROSTANE-3,17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANO:5-BETA-ANDROSTANE-3,17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o5x\|2o5x]], [[2o5y\|2o5y]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o5x\|2o5x]], [[2o5y\|2o5y]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [http://www.ebi.ac.uk/pdbsum/2o5z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [http://www.ebi.ac.uk/pdbsum/2o5z PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
-	[[Category: Verdino, P.]]	+	[[Category: Verdino, P]]
-	[[Category: Wilson, I A.]]	+	[[Category: Wilson, I A]]
	[[Category: Antibody engineering]]		[[Category: Antibody engineering]]
	[[Category: Chimeric fab]]		[[Category: Chimeric fab]]

OCA at 10:58, 3 October 2014

OCA — Fri, 03 Oct 2014 10:58:38 GMT

←Older revision		Revision as of 10:58, 3 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2o5z\| PDB=2o5z \| SCENE= }}~~	+	==Crystal structure of the 1E9 LeuH47Trp/ArgH100Trp Fab 5-beta-androstane-3,17-dione complex==
-	===Crystal structure of the 1E9 LeuH47Trp/ArgH100Trp Fab 5-beta-androstane-3,17-dione complex===	+	<StructureSection load='2o5z' size='340' side='right' caption='[[2o5z]], [[Resolution\|resolution]] 2.40Å' scene=''>
-	~~{{ABSTRACT_PUBMED_18689687}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2o5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2O5Z FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANO:5-BETA-ANDROSTANE-3,17-DIONE'>ANO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2o5x\|2o5x]], [[2o5y\|2o5y]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o5z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2o5z RCSB], [http://www.ebi.ac.uk/pdbsum/2o5z PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o5/2o5z_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Molecular recognition by the adaptive immune system relies on specific high-affinity antibody receptors that are generated from a restricted set of starting sequences through homologous recombination and somatic mutation. The steroid binding antibody DB3 and the catalytic Diels-Alderase antibody 1E9 derive from the same germ line sequences but exhibit very distinct specificities and functions. However, mutation of only two of the 36 sequence differences in the variable domains, Leu(H47)Trp and Arg(H100)Trp, converts 1E9 into a high-affinity steroid receptor with a ligand recognition profile similar to DB3. To understand how these changes switch binding specificity and function, we determined the crystal structures of the 1E9 Leu(H47)Trp/Arg(H100)Trp double mutant (1E9dm) as an unliganded Fab at 2.05 A resolution and in complex with two configurationally distinct steroids at 2.40 and 2.85 A. Surprisingly, despite the functional mimicry of DB3, 1E9dm employs a distinct steroid binding mechanism. Extensive structural rearrangements occur in the combining site, where residue H47 acts as a specificity switch and H100 adapts to different ligands. Unlike DB3, 1E9dm does not use alternative binding pockets or different sets of hydrogen-bonding interactions to bind configurationally distinct steroids. Rather, the different steroids are inserted more deeply into the 1E9dm combining site, creating more hydrophobic contacts that energetically compensate for the lack of hydrogen bonds. These findings demonstrate how subtle mutations within an existing molecular scaffold can dramatically modulate the function of immune receptors by inducing unanticipated, but compensating, mechanisms of ligand interaction.

-	~~==About this Structure==~~	+	Closely related antibody receptors exploit fundamentally different strategies for steroid recognition.,Verdino P, Aldag C, Hilvert D, Wilson IA Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11725-30. Epub 2008 Aug 8. PMID:18689687<ref>PMID:18689687</ref>
-	~~[[2o5z]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2O5Z OCA].~~	+

-	~~==See Also==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	*[[Antibody\|Antibody]]	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:018689687</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Verdino, P.]]		[[Category: Verdino, P.]]

OCA at 08:42, 25 December 2013

OCA — Wed, 25 Dec 2013 08:42:54 GMT

←Older revision		Revision as of 08:42, 25 December 2013
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	==About this Structure==		==About this Structure==
-	[[2o5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O5Z OCA].	+	[[2o5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O5Z OCA].

	==See Also==		==See Also==
Line 11:		Line 11:
	==Reference==		==Reference==
	<ref group="xtra">PMID:018689687</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:018689687</ref><references group="xtra"/><references/>
-	[[Category: ~~Homo sapiens]]~~	+	[[Category: Lk3 transgenic mice]]
-	~~[[Category: Mus musculus~~]]	+
	[[Category: Verdino, P.]]		[[Category: Verdino, P.]]
	[[Category: Wilson, I A.]]		[[Category: Wilson, I A.]]

OCA at 05:08, 25 March 2013

OCA — Mon, 25 Mar 2013 05:08:14 GMT

←Older revision		Revision as of 05:08, 25 March 2013
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-	[[Image:2o5z.png\|left\|200px]]
-
	{{STRUCTURE_2o5z\| PDB=2o5z \| SCENE= }}		{{STRUCTURE_2o5z\| PDB=2o5z \| SCENE= }}
-
	===Crystal structure of the 1E9 LeuH47Trp/ArgH100Trp Fab 5-beta-androstane-3,17-dione complex===		===Crystal structure of the 1E9 LeuH47Trp/ArgH100Trp Fab 5-beta-androstane-3,17-dione complex===
-
	{{ABSTRACT_PUBMED_18689687}}		{{ABSTRACT_PUBMED_18689687}}

Line 14:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:018689687</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018689687</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]