2ofp - Revision history

OCA at 10:37, 30 August 2023

OCA — Wed, 30 Aug 2023 10:37:07 GMT

←Older revision		Revision as of 10:37, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='2ofp' size='340' side='right'caption='[[2ofp]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2ofp' size='340' side='right'caption='[[2ofp]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ofp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OFP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ofp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OFP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PAF:PANTOATE'>PAF</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ks9\|1ks9]], [[1yjq\|1yjq]], [[1yon\|1yon]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panE, apbA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/2-dehydropantoate_2-reductase 2-dehydropantoate 2-reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.169 1.1.1.169] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ofp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofp OCA], [https://pdbe.org/2ofp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ofp RCSB], [https://www.ebi.ac.uk/pdbsum/2ofp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ofp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ofp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofp OCA], [https://pdbe.org/2ofp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ofp RCSB], [https://www.ebi.ac.uk/pdbsum/2ofp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ofp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PANE_ECOLI PANE_ECOLI]] Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.	+	[https://www.uniprot.org/uniprot/PANE_ECOLI PANE_ECOLI] Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 2-~~dehydropantoate 2-reductase]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Ecoli~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abell, C]]	+	[[Category: Abell C]]
-	[[Category: Blundell~~, T L~~]]	+	[[Category: Blundell TL]]
-	[[Category: Chirgadze~~, D Y~~]]	+	[[Category: Chirgadze DY]]
-	[[Category: Ciulli, A]]	+	[[Category: Ciulli A]]
-	[[Category: Smith~~, A G]]~~	+	[[Category: Smith AG]]
-	~~[[Category: Apba]]~~	+
-	~~[[Category: Ketopantoate reductase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Pane]]~~	+
-	~~[[Category: Ternary complex~~]]	+

OCA at 12:55, 9 June 2021

OCA — Wed, 09 Jun 2021 12:55:16 GMT

←Older revision		Revision as of 12:55, 9 June 2021
Line 1:		Line 1:

	==Crystal structure of Escherichia coli ketopantoate reductase in a ternary complex with NADP+ and pantoate==		==Crystal structure of Escherichia coli ketopantoate reductase in a ternary complex with NADP+ and pantoate==
-	<StructureSection load='2ofp' size='340' side='right' caption='[[2ofp]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='2ofp' size='340' side='right'caption='[[2ofp]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ofp]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2OFP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ofp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OFP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ks9\|1ks9]], [[1yjq\|1yjq]], [[1yon\|1yon]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ks9\|1ks9]], [[1yjq\|1yjq]], [[1yon\|1yon]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panE, apbA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panE, apbA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/2-dehydropantoate_2-reductase 2-dehydropantoate 2-reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.169 1.1.1.169] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/2-dehydropantoate_2-reductase 2-dehydropantoate 2-reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.169 1.1.1.169] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ofp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofp OCA], [~~http~~://pdbe.org/2ofp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ofp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ofp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ofp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ofp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofp OCA], [https://pdbe.org/2ofp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ofp RCSB], [https://www.ebi.ac.uk/pdbsum/2ofp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ofp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PANE_ECOLI PANE_ECOLI]] Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.	+	[[https://www.uniprot.org/uniprot/PANE_ECOLI PANE_ECOLI]] Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 37:
	[[Category: 2-dehydropantoate 2-reductase]]		[[Category: 2-dehydropantoate 2-reductase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Abell, C]]		[[Category: Abell, C]]
	[[Category: Blundell, T L]]		[[Category: Blundell, T L]]

OCA at 07:21, 22 August 2018

OCA — Wed, 22 Aug 2018 07:21:33 GMT

←Older revision		Revision as of 07:21, 22 August 2018
Line 1:		Line 1:
		+
	==Crystal structure of Escherichia coli ketopantoate reductase in a ternary complex with NADP+ and pantoate==		==Crystal structure of Escherichia coli ketopantoate reductase in a ternary complex with NADP+ and pantoate==
	<StructureSection load='2ofp' size='340' side='right' caption='[[2ofp]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2ofp' size='340' side='right' caption='[[2ofp]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panE, apbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panE, apbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-dehydropantoate_2-reductase 2-dehydropantoate 2-reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.169 1.1.1.169] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-dehydropantoate_2-reductase 2-dehydropantoate 2-reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.169 1.1.1.169] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ofp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofp OCA], [http://pdbe.org/2ofp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ofp RCSB], [http://www.ebi.ac.uk/pdbsum/2ofp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ofp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofp OCA], [http://pdbe.org/2ofp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ofp RCSB], [http://www.ebi.ac.uk/pdbsum/2ofp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ofp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/2ofp_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/2ofp_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:36, 10 February 2016

OCA — Wed, 10 Feb 2016 08:36:50 GMT

←Older revision		Revision as of 08:36, 10 February 2016
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ofp ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:09, 11 September 2015

OCA — Fri, 11 Sep 2015 07:09:34 GMT

←Older revision		Revision as of 07:09, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2ofp' size='340' side='right' caption='[[2ofp]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2ofp' size='340' side='right' caption='[[2ofp]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ofp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OFP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ofp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OFP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ks9\|1ks9]], [[1yjq\|1yjq]], [[1yon\|1yon]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ks9\|1ks9]], [[1yjq\|1yjq]], [[1yon\|1yon]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panE, apbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ~~Escherichia coli K-12~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panE, apbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-dehydropantoate_2-reductase 2-dehydropantoate 2-reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.169 1.1.1.169] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-dehydropantoate_2-reductase 2-dehydropantoate 2-reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.169 1.1.1.169] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ofp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ofp RCSB], [http://www.ebi.ac.uk/pdbsum/2ofp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ofp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofp OCA], [http://pdbe.org/2ofp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ofp RCSB], [http://www.ebi.ac.uk/pdbsum/2ofp PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ofp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: 2-dehydropantoate 2-reductase]]		[[Category: 2-dehydropantoate 2-reductase]]
-	[[Category: ~~Escherichia coli k-12~~]]	+	[[Category: Ecoli]]
	[[Category: Abell, C]]		[[Category: Abell, C]]
	[[Category: Blundell, T L]]		[[Category: Blundell, T L]]

OCA at 13:02, 25 December 2014

OCA — Thu, 25 Dec 2014 13:02:49 GMT

←Older revision		Revision as of 13:02, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ofp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OFP FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ofp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OFP FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PAF:PANTOATE'>PAF</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ks9\|1ks9]], [[1yjq\|1yjq]], [[1yon\|1yon]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ks9\|1ks9]], [[1yjq\|1yjq]], [[1yon\|1yon]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panE, apbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panE, apbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-dehydropantoate_2-reductase 2-dehydropantoate 2-reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.169 1.1.1.169] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-dehydropantoate_2-reductase 2-dehydropantoate 2-reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.169 1.1.1.169] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ofp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ofp RCSB], [http://www.ebi.ac.uk/pdbsum/2ofp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ofp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ofp RCSB], [http://www.ebi.ac.uk/pdbsum/2ofp PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PANE_ECOLI PANE_ECOLI]] Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	[[Category: 2-dehydropantoate 2-reductase]]		[[Category: 2-dehydropantoate 2-reductase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
-	[[Category: Abell, C.]]	+	[[Category: Abell, C]]
-	[[Category: Blundell, T L.]]	+	[[Category: Blundell, T L]]
-	[[Category: Chirgadze, D Y.]]	+	[[Category: Chirgadze, D Y]]
-	[[Category: Ciulli, A.]]	+	[[Category: Ciulli, A]]
-	[[Category: Smith, A G.]]	+	[[Category: Smith, A G]]
	[[Category: Apba]]		[[Category: Apba]]
	[[Category: Ketopantoate reductase]]		[[Category: Ketopantoate reductase]]

OCA at 20:14, 30 September 2014

OCA — Tue, 30 Sep 2014 20:14:01 GMT

←Older revision		Revision as of 20:14, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2ofp.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of Escherichia coli ketopantoate reductase in a ternary complex with NADP+ and pantoate==
		+	<StructureSection load='2ofp' size='340' side='right' caption='[[2ofp]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ofp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OFP FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PAF:PANTOATE'>PAF</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ks9\|1ks9]], [[1yjq\|1yjq]], [[1yon\|1yon]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">panE, apbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-dehydropantoate_2-reductase 2-dehydropantoate 2-reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.169 1.1.1.169] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ofp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ofp RCSB], [http://www.ebi.ac.uk/pdbsum/2ofp PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/2ofp_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ketopantoate reductase (KPR, EC 1.1.1.169) catalyzes the NADPH-dependent reduction of ketopantoate to pantoate, an essential step for the biosynthesis of pantothenate (vitamin B5). Inhibitors of the enzymes of this pathway have been proposed as potential antibiotics or herbicides. Here we present the crystal structure of Escherichia coli KPR in a precatalytic ternary complex with NADP+ and pantoate bound, solved to 2.3 A of resolution. The asymmetric unit contains two protein molecules, each in a ternary complex; however, one is in a more closed conformation than the other. A hinge bending between the N- and C-terminal domains is observed, which triggers the switch of the essential Lys176 to form a key hydrogen bond with the C2 hydroxyl of pantoate. Pantoate forms additional interactions with conserved residues Ser244, Asn98, and Asn180 and with two conservatively varied residues, Asn194 and Asn241. The steady-state kinetics of active site mutants R31A, K72A, N98A, K176A, S244A, and E256A implicate Asn98 as well as Lys176 and Glu256 in the catalytic mechanism. Isothermal titration calorimetry studies with these mutants further demonstrate the importance of Ser244 for substrate binding and of Arg31 and Lys72 for cofactor binding. Further calorimetric studies show that KPR discriminates binding of ketopantoate against pantoate only with NADPH bound. This work provides insights into the roles of active site residues and conformational changes in substrate recognition and catalysis, leading to the proposal of a detailed molecular mechanism for KPR activity.

-	~~{{STRUCTURE_2ofp\| PDB=2ofp \| SCENE= }}~~	+	Crystal structure of Escherichia coli ketopantoate reductase in a ternary complex with NADP+ and pantoate bound: substrate recognition, conformational change, and cooperativity.,Ciulli A, Chirgadze DY, Smith AG, Blundell TL, Abell C J Biol Chem. 2007 Mar 16;282(11):8487-97. Epub 2007 Jan 16. PMID:17229734<ref>PMID:17229734</ref>

-	~~===Crystal structure~~ of ~~Escherichia coli ketopantoate reductase in a ternary complex with NADP+ and pantoate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17229734}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2ofp]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017229734<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: 2-dehydropantoate 2-reductase]]		[[Category: 2-dehydropantoate 2-reductase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]

OCA: Protected "2ofp" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 12:35:57 GMT

Protected "2ofp" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:35, 7 January 2013

OCA at 12:35, 7 January 2013

OCA — Mon, 07 Jan 2013 12:35:52 GMT

←Older revision		Revision as of 12:35, 7 January 2013
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-	{{Seed}}
	[[Image:2ofp.png\|left\|200px]]		[[Image:2ofp.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2ofp", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_2ofp\| PDB=2ofp \| SCENE= }}		{{STRUCTURE_2ofp\| PDB=2ofp \| SCENE= }}

	===Crystal structure of Escherichia coli ketopantoate reductase in a ternary complex with NADP+ and pantoate===		===Crystal structure of Escherichia coli ketopantoate reductase in a ternary complex with NADP+ and pantoate===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17229734}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17229734 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17229734}}		{{ABSTRACT_PUBMED_17229734}}

	==About this Structure==		==About this Structure==
-	~~2OFP~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Escherichia_coli~~ Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA].	+	[[2ofp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17229734~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017229734</ref><references group="xtra"/>
	[[Category: 2-dehydropantoate 2-reductase]]		[[Category: 2-dehydropantoate 2-reductase]]
-	[[Category: Escherichia coli]]	+	[[Category: Escherichia coli k-12]]
	[[Category: Abell, C.]]		[[Category: Abell, C.]]
	[[Category: Blundell, T L.]]		[[Category: Blundell, T L.]]
Line 33:		Line 21:
	[[Category: Apba]]		[[Category: Apba]]
	[[Category: Ketopantoate reductase]]		[[Category: Ketopantoate reductase]]
		+	[[Category: Oxidoreductase]]
	[[Category: Pane]]		[[Category: Pane]]
	[[Category: Ternary complex]]		[[Category: Ternary complex]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:53:01 2009''

OCA at 14:53, 17 February 2009

OCA — Tue, 17 Feb 2009 14:53:01 GMT

←Older revision		Revision as of 14:53, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2OFP is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA].	+	2OFP is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFP OCA].

	==Reference==		==Reference==
-	Crystal structure of Escherichia coli ketopantoate reductase in a ternary complex with NADP+ and pantoate bound: substrate recognition, conformational change, and cooperativity., Ciulli A, Chirgadze DY, Smith AG, Blundell TL, Abell C, J Biol Chem. 2007 Mar 16;282(11):8487-97. Epub 2007 Jan 16. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17229734 17229734]~~	+	<ref group="xtra">PMID:17229734</ref><references group="xtra"/>
	[[Category: 2-dehydropantoate 2-reductase]]		[[Category: 2-dehydropantoate 2-reductase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Abell, C.]]		[[Category: Abell, C.]]
	[[Category: Blundell, T L.]]		[[Category: Blundell, T L.]]
Line 37:		Line 36:
	[[Category: Ternary complex]]		[[Category: Ternary complex]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 23~~:41:~~06 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:53:01 2009''