2pco - Revision history

OCA at 12:53, 20 December 2023

OCA — Wed, 20 Dec 2023 12:53:35 GMT

←Older revision		Revision as of 12:53, 20 December 2023
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	==Spatial Structure and Membrane Permeabilization for Latarcin-1, a Spider Antimicrobial Peptide==		==Spatial Structure and Membrane Permeabilization for Latarcin-1, a Spider Antimicrobial Peptide==
-	<StructureSection load='2pco' size='340' side='right'caption='[[2pco~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2pco' size='340' side='right'caption='[[2pco]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PCO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachesana_tarabaevi Lachesana tarabaevi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PCO FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[2g9p\|2g9p]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [https://pdbe.org/2pco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [https://www.ebi.ac.uk/pdbsum/2pco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pco ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [https://pdbe.org/2pco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [https://www.ebi.ac.uk/pdbsum/2pco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pco ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LAT1_LACTA LAT1_LACTA]] Has antimicrobial activity against Gram-positive bacteria (A.globiformis VKM Ac-1112 (MIC=0.5 ug/ml), and B.subtilis VKM B-501 (MIC=1.0 ug/ml)), Gram-negative bacteria (E.coli DH5-alpha (MIC=1.0 ug/ml), E.coli MH1 (MIC=0.7 ug/ml), and P.aeruginosa PAO1 (MIC=4.1 ug/ml)), and yeasts (P.pastoris GS115 (MIC=17 ug/ml), and S.cerevisiae Y190 (MIC>33 ug/ml)). Has a moderate hemolytic activity against rabbit erythrocytes. Causes paralysis, but is not lethal when injected into insect (Musca domestica) larvae.	+	[https://www.uniprot.org/uniprot/LAT1_LACTA LAT1_LACTA] Has antimicrobial activity against Gram-positive bacteria (A.globiformis VKM Ac-1112 (MIC=0.5 ug/ml), and B.subtilis VKM B-501 (MIC=1.0 ug/ml)), Gram-negative bacteria (E.coli DH5-alpha (MIC=1.0 ug/ml), E.coli MH1 (MIC=0.7 ug/ml), and P.aeruginosa PAO1 (MIC=4.1 ug/ml)), and yeasts (P.pastoris GS115 (MIC=17 ug/ml), and S.cerevisiae Y190 (MIC>33 ug/ml)). Has a moderate hemolytic activity against rabbit erythrocytes. Causes paralysis, but is not lethal when injected into insect (Musca domestica) larvae.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lachesana tarabaevi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Arseniev~~, A S~~]]	+	[[Category: Arseniev AS]]
-	[[Category: Chupin~~, V V~~]]	+	[[Category: Chupin VV]]
-	[[Category: Dubovskii~~, P V~~]]	+	[[Category: Dubovskii PV]]
-	[[Category: Efremov~~, R G~~]]	+	[[Category: Efremov RG]]
-	[[Category: Polyansky~~, A A~~]]	+	[[Category: Polyansky AA]]
-	[[Category: Volynsky~~, P E]]~~	+	[[Category: Volynsky PE]]
-	~~[[Category: Continuous helix]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 07:44, 9 March 2022

OCA — Wed, 09 Mar 2022 07:44:40 GMT

←Older revision		Revision as of 07:44, 9 March 2022
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	<StructureSection load='2pco' size='340' side='right'caption='[[2pco]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2pco' size='340' side='right'caption='[[2pco]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2PCO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PCO FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g9p\|2g9p]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2g9p\|2g9p]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [~~http~~://pdbe.org/2pco PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2pco PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2pco ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [https://pdbe.org/2pco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [https://www.ebi.ac.uk/pdbsum/2pco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pco ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LAT1_LACTA LAT1_LACTA]] Has antimicrobial activity against Gram-positive bacteria (A.globiformis VKM Ac-1112 (MIC=0.5 ug/ml), and B.subtilis VKM B-501 (MIC=1.0 ug/ml)), Gram-negative bacteria (E.coli DH5-alpha (MIC=1.0 ug/ml), E.coli MH1 (MIC=0.7 ug/ml), and P.aeruginosa PAO1 (MIC=4.1 ug/ml)), and yeasts (P.pastoris GS115 (MIC=17 ug/ml), and S.cerevisiae Y190 (MIC>33 ug/ml)). Has a moderate hemolytic activity against rabbit erythrocytes. Causes paralysis, but is not lethal when injected into insect (Musca domestica) larvae.	+	[[https://www.uniprot.org/uniprot/LAT1_LACTA LAT1_LACTA]] Has antimicrobial activity against Gram-positive bacteria (A.globiformis VKM Ac-1112 (MIC=0.5 ug/ml), and B.subtilis VKM B-501 (MIC=1.0 ug/ml)), Gram-negative bacteria (E.coli DH5-alpha (MIC=1.0 ug/ml), E.coli MH1 (MIC=0.7 ug/ml), and P.aeruginosa PAO1 (MIC=4.1 ug/ml)), and yeasts (P.pastoris GS115 (MIC=17 ug/ml), and S.cerevisiae Y190 (MIC>33 ug/ml)). Has a moderate hemolytic activity against rabbit erythrocytes. Causes paralysis, but is not lethal when injected into insect (Musca domestica) larvae.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 06:54, 3 June 2020

OCA — Wed, 03 Jun 2020 06:54:08 GMT

←Older revision		Revision as of 06:54, 3 June 2020
Line 1:		Line 1:

	==Spatial Structure and Membrane Permeabilization for Latarcin-1, a Spider Antimicrobial Peptide==		==Spatial Structure and Membrane Permeabilization for Latarcin-1, a Spider Antimicrobial Peptide==
-	<StructureSection load='2pco' size='340' side='right' caption='[[2pco]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2pco' size='340' side='right'caption='[[2pco]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2PCO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2PCO FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g9p\|2g9p]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g9p\|2g9p]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [http://pdbe.org/2pco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [http://www.ebi.ac.uk/pdbsum/2pco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pco ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [http://pdbe.org/2pco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [http://www.ebi.ac.uk/pdbsum/2pco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pco ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Arseniev, A S]]		[[Category: Arseniev, A S]]
	[[Category: Chupin, V V]]		[[Category: Chupin, V V]]

OCA at 08:47, 29 August 2018

OCA — Wed, 29 Aug 2018 08:47:29 GMT

←Older revision		Revision as of 08:47, 29 August 2018
Line 1:		Line 1:
		+
	==Spatial Structure and Membrane Permeabilization for Latarcin-1, a Spider Antimicrobial Peptide==		==Spatial Structure and Membrane Permeabilization for Latarcin-1, a Spider Antimicrobial Peptide==
	<StructureSection load='2pco' size='340' side='right' caption='[[2pco]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2pco' size='340' side='right' caption='[[2pco]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PCO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PCO FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g9p\|2g9p]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g9p\|2g9p]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [http://pdbe.org/2pco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [http://www.ebi.ac.uk/pdbsum/2pco PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [http://pdbe.org/2pco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [http://www.ebi.ac.uk/pdbsum/2pco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pco ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:50, 11 September 2015

OCA — Fri, 11 Sep 2015 17:50:49 GMT

←Older revision		Revision as of 17:50, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PCO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PCO FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g9p\|2g9p]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g9p\|2g9p]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [http://www.ebi.ac.uk/pdbsum/2pco PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [http://pdbe.org/2pco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [http://www.ebi.ac.uk/pdbsum/2pco PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2pco" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:49, 25 December 2014

OCA — Thu, 25 Dec 2014 19:49:08 GMT

←Older revision		Revision as of 19:49, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PCO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PCO FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g9p\|2g9p]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g9p\|2g9p]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [http://www.ebi.ac.uk/pdbsum/2pco PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [http://www.ebi.ac.uk/pdbsum/2pco PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LAT1_LACTA LAT1_LACTA]] Has antimicrobial activity against Gram-positive bacteria (A.globiformis VKM Ac-1112 (MIC=0.5 ug/ml), and B.subtilis VKM B-501 (MIC=1.0 ug/ml)), Gram-negative bacteria (E.coli DH5-alpha (MIC=1.0 ug/ml), E.coli MH1 (MIC=0.7 ug/ml), and P.aeruginosa PAO1 (MIC=4.1 ug/ml)), and yeasts (P.pastoris GS115 (MIC=17 ug/ml), and S.cerevisiae Y190 (MIC>33 ug/ml)). Has a moderate hemolytic activity against rabbit erythrocytes. Causes paralysis, but is not lethal when injected into insect (Musca domestica) larvae.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Arseniev, A S.]]	+	[[Category: Arseniev, A S]]
-	[[Category: Chupin, V V.]]	+	[[Category: Chupin, V V]]
-	[[Category: Dubovskii, P V.]]	+	[[Category: Dubovskii, P V]]
-	[[Category: Efremov, R G.]]	+	[[Category: Efremov, R G]]
-	[[Category: Polyansky, A A.]]	+	[[Category: Polyansky, A A]]
-	[[Category: Volynsky, P E.]]	+	[[Category: Volynsky, P E]]
	[[Category: Continuous helix]]		[[Category: Continuous helix]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 07:05, 9 June 2014

OCA — Mon, 09 Jun 2014 07:05:17 GMT

←Older revision		Revision as of 07:05, 9 June 2014
Line 1:		Line 1:
-	[[~~Image:~~2pco.~~png~~\|~~left~~\|~~200px~~]]	+	==Spatial Structure and Membrane Permeabilization for Latarcin-1, a Spider Antimicrobial Peptide==
		+	<StructureSection load='2pco' size='340' side='right' caption='[[2pco]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PCO FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2g9p\|2g9p]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pco OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pco RCSB], [http://www.ebi.ac.uk/pdbsum/2pco PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Latarcins, linear peptides from the Lachesana tarabaevi spider venom, exhibit a broad-spectrum antimicrobial activity, likely acting on the bacterial cytoplasmic membrane. We study their spatial structures and interaction with model membranes by a combination of experimental and theoretical methods to reveal the structure-activity relationship. In this work, a 26 amino acid peptide, Ltc1, was investigated. Its spatial structure in detergent micelles was determined by (1)H nuclear magnetic resonance (NMR) and refined by Monte Carlo simulations in an implicit water-octanol slab. The Ltc1 molecule was found to form a straight uninterrupted amphiphilic helix comprising 8-23 residues. A dye-leakage fluorescent assay and (31)P NMR spectroscopy established that the peptide does not induce the release of fluorescent marker nor deteriorate the bilayer structure of the membranes. The voltage-clamp technique showed that Ltc1 induces the current fluctuations through planar membranes when the sign of the applied potential coincides with the one across the bacterial inner membrane. This implies that Ltc1 acts on the membranes via a specific mechanism, which is different from the carpet mode demonstrated by another latarcin, Ltc2a, featuring a helix-hinge-helix structure with a hydrophobicity gradient along the peptide chain. In contrast, the hydrophobic surface of the Ltc1 helix is narrow-shaped and extends with no gradient along the axis. We have also disclosed a number of peptides, structurally homologous to Ltc1 and exhibiting similar membrane activity. This indicates that the hydrophobic pattern of the Ltc1 helix and related antimicrobial peptides specifies their activity mechanism. The latter assumes the formation of variable-sized lesions, which depend upon the potential across the membrane.

-	~~{{STRUCTURE_2pco\| PDB=2pco \| SCENE= }}~~	+	Three-dimensional structure/hydrophobicity of latarcins specifies their mode of membrane activity(,).,Dubovskii PV, Volynsky PE, Polyansky AA, Karpunin DV, Chupin VV, Efremov RG, Arseniev AS Biochemistry. 2008 Mar 18;47(11):3525-33. Epub 2008 Feb 23. PMID:18293934<ref>PMID:18293934</ref>

-	~~===Spatial Structure and Membrane Permeabilization for Latarcin-1~~, a ~~Spider Antimicrobial Peptide===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18293934}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2pco]] is a 1 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2PCO OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018293934<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Arseniev, A S.]]		[[Category: Arseniev, A S.]]
	[[Category: Chupin, V V.]]		[[Category: Chupin, V V.]]

OCA: Protected "2pco" [edit=sysop:move=sysop]

OCA — Tue, 14 Aug 2012 18:51:21 GMT

Protected "2pco" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:51, 14 August 2012

OCA at 18:51, 14 August 2012

OCA — Tue, 14 Aug 2012 18:51:19 GMT

←Older revision		Revision as of 18:51, 14 August 2012
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-	{{Seed}}
	[[Image:2pco.png\|left\|200px]]		[[Image:2pco.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2pco", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2pco\| PDB=2pco \| SCENE= }}		{{STRUCTURE_2pco\| PDB=2pco \| SCENE= }}

	===Spatial Structure and Membrane Permeabilization for Latarcin-1, a Spider Antimicrobial Peptide===		===Spatial Structure and Membrane Permeabilization for Latarcin-1, a Spider Antimicrobial Peptide===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18293934}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18293934 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18293934}}		{{ABSTRACT_PUBMED_18293934}}

	==About this Structure==		==About this Structure==
-	~~2PCO is a~~ [[~~Single protein~~]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA].	+	[[2pco]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA].

	==Reference==		==Reference==
-	Three-dimensional structure/hydrophobicity of latarcins specifies their mode of membrane activity(,)., Dubovskii PV, Volynsky PE, Polyansky AA, Karpunin DV, Chupin VV, Efremov RG, Arseniev AS, Biochemistry. 2008 Mar 18;47(11):3525-33. Epub 2008 Feb 23. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18293934 18293934]~~	+	<ref group="xtra">PMID:018293934</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Arseniev, A S.]]		[[Category: Arseniev, A S.]]
	[[Category: Chupin, V V.]]		[[Category: Chupin, V V.]]
Line 33:		Line 20:
	[[Category: Continuous helix]]		[[Category: Continuous helix]]
	[[Category: Toxin]]		[[Category: Toxin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:52:52 2008''

OCA at 08:52, 29 July 2008

OCA — Tue, 29 Jul 2008 08:52:57 GMT

←Older revision		Revision as of 08:52, 29 July 2008
Line 1:		Line 1:
-	[[Image:2pco.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2pco.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2pco\| PDB=2pco \| SCENE= }}		{{STRUCTURE_2pco\| PDB=2pco \| SCENE= }}

-	~~'''~~Spatial Structure and Membrane Permeabilization for Latarcin-1, a Spider Antimicrobial Peptide~~'''~~	+	===Spatial Structure and Membrane Permeabilization for Latarcin-1, a Spider Antimicrobial Peptide===


-	~~==Overview==~~	+	<!--
-	~~Latarcins~~, ~~linear peptides from the Lachesana tarabaevi spider venom~~, ~~exhibit a broad-spectrum antimicrobial activity, likely acting on~~ the ~~bacterial cytoplasmic membrane. We study their spatial structures and interaction with model membranes by a combination of experimental and theoretical methods~~ to ~~reveal~~ the ~~structure-activity relationship. In this work, a 26 amino acid peptide, Ltc1, was investigated. Its spatial structure in detergent micelles was determined by~~ (~~1)H nuclear magnetic resonance (NMR) and refined by Monte Carlo simulations in an implicit water-octanol slab~~. ~~The Ltc1 molecule was found to form a straight uninterrupted amphiphilic helix comprising 8-23 residues~~. ~~A dye-leakage fluorescent assay and (31~~)P NMR spectroscopy established that the peptide does not induce the release of fluorescent marker nor deteriorate the bilayer structure of the membranes. The voltage-clamp technique showed that Ltc1 induces the current fluctuations through planar membranes when the sign of the applied potential coincides with the one across the bacterial inner membrane. This implies that Ltc1 acts on the membranes via a specific mechanism, ~~which~~ is ~~different from~~ the carpet mode demonstrated by another latarcin, Ltc2a, featuring a helix-hinge-helix structure with a hydrophobicity gradient along the peptide chain. In contrast, the hydrophobic surface of the Ltc1 helix is narrow-shaped and extends with no gradient along the axis. We have also disclosed a number ~~of peptides, structurally homologous to Ltc1 and exhibiting similar membrane activity~~. ~~This indicates that the hydrophobic pattern of the Ltc1 helix and related antimicrobial peptides specifies their activity mechanism. The latter assumes the formation of variable~~-~~sized lesions, which depend upon the potential across the membrane.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_18293934}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18293934 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18293934}}

	==About this Structure==		==About this Structure==
-	2PCO is a [[Single protein]] structure. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA].	+	2PCO is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PCO OCA].

	==Reference==		==Reference==
Line 29:		Line 33:
	[[Category: Continuous helix]]		[[Category: Continuous helix]]
	[[Category: Toxin]]		[[Category: Toxin]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 12~~:51:39 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:52:52 2008''