2ps0 - Revision history

OCA at 15:31, 17 June 2021

OCA — Thu, 17 Jun 2021 15:31:29 GMT

←Older revision		Revision as of 15:31, 17 June 2021
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	<StructureSection load='2ps0' size='340' side='right'caption='[[2ps0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2ps0' size='340' side='right'caption='[[2ps0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ps0]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2PS0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ps0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PS0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ogw\|2ogw]], [[2osv\|2osv]], [[2prs\|2prs]], [[2ps3\|2ps3]], [[2ps9\|2ps9]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ogw\|2ogw]], [[2osv\|2osv]], [[2prs\|2prs]], [[2ps3\|2ps3]], [[2ps9\|2ps9]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ps0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ps0 OCA], [~~http~~://pdbe.org/2ps0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2ps0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2ps0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2ps0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ps0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ps0 OCA], [https://pdbe.org/2ps0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ps0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ps0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ps0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ZNUA_ECOLI ZNUA_ECOLI]] Involved in the high-affinity zinc uptake transport system.	+	[[https://www.uniprot.org/uniprot/ZNUA_ECOLI ZNUA_ECOLI]] Involved in the high-affinity zinc uptake transport system.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:49, 31 July 2019

OCA — Wed, 31 Jul 2019 07:49:51 GMT

←Older revision		Revision as of 07:49, 31 July 2019
Line 1:		Line 1:

	==Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli==		==Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli==
-	<StructureSection load='2ps0' size='340' side='right' caption='[[2ps0]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='2ps0' size='340' side='right'caption='[[2ps0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ps0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PS0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ps0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PS0 FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/2ps0_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/2ps0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Kim, L R]]		[[Category: Kim, L R]]
	[[Category: Rosenzweig, A C]]		[[Category: Rosenzweig, A C]]

OCA at 09:32, 18 October 2017

OCA — Wed, 18 Oct 2017 09:32:21 GMT

←Older revision		Revision as of 09:32, 18 October 2017
Line 1:		Line 1:
		+
	==Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli==		==Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli==
	<StructureSection load='2ps0' size='340' side='right' caption='[[2ps0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2ps0' size='340' side='right' caption='[[2ps0]], [[Resolution\|resolution]] 2.00Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ogw\|2ogw]], [[2osv\|2osv]], [[2prs\|2prs]], [[2ps3\|2ps3]], [[2ps9\|2ps9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ogw\|2ogw]], [[2osv\|2osv]], [[2prs\|2prs]], [[2ps3\|2ps3]], [[2ps9\|2ps9]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ps0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ps0 OCA], [http://pdbe.org/2ps0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ps0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ps0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ps0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ps0 OCA], [http://pdbe.org/2ps0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ps0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ps0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ps0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ps0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:28, 11 September 2015

OCA — Fri, 11 Sep 2015 22:28:15 GMT

←Older revision		Revision as of 22:28, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2ps0' size='340' side='right' caption='[[2ps0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2ps0' size='340' side='right' caption='[[2ps0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2ps0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PS0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2ps0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PS0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ogw\|2ogw]], [[2osv\|2osv]], [[2prs\|2prs]], [[2ps3\|2ps3]], [[2ps9\|2ps9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ogw\|2ogw]], [[2osv\|2osv]], [[2prs\|2prs]], [[2ps3\|2ps3]], [[2ps9\|2ps9]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ps0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ps0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ps0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ps0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ps0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ps0 OCA], [http://pdbe.org/2ps0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ps0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ps0 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2ps0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Kim, L R]]		[[Category: Kim, L R]]
	[[Category: Rosenzweig, A C]]		[[Category: Rosenzweig, A C]]

OCA at 20:17, 25 December 2014

OCA — Thu, 25 Dec 2014 20:17:45 GMT

←Older revision		Revision as of 20:17, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2ps0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PS0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2ps0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PS0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ogw\|2ogw]], [[2osv\|2osv]], [[2prs\|2prs]], [[2ps3\|2ps3]], [[2ps9\|2ps9]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ogw\|2ogw]], [[2osv\|2osv]], [[2prs\|2prs]], [[2ps3\|2ps3]], [[2ps9\|2ps9]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ps0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ps0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ps0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ps0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ps0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ps0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ps0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ps0 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ZNUA_ECOLI ZNUA_ECOLI]] Involved in the high-affinity zinc uptake transport system.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Kim, L R.]]	+	[[Category: Kim, L R]]
-	[[Category: Rosenzweig, A C.]]	+	[[Category: Rosenzweig, A C]]
-	[[Category: Vorontsov, I I.]]	+	[[Category: Vorontsov, I I]]
-	[[Category: Yatsunyk, L A.]]	+	[[Category: Yatsunyk, L A]]
	[[Category: Metal transport]]		[[Category: Metal transport]]

OCA at 19:33, 30 September 2014

OCA — Tue, 30 Sep 2014 19:33:54 GMT

←Older revision		Revision as of 19:33, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2ps0.~~png~~\|~~left~~\|200px]]	+	==Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli==
		+	<StructureSection load='2ps0' size='340' side='right' caption='[[2ps0]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2ps0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PS0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ogw\|2ogw]], [[2osv\|2osv]], [[2prs\|2prs]], [[2ps3\|2ps3]], [[2ps9\|2ps9]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ps0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ps0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ps0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ps0 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/2ps0_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ZnuA is the periplasmic Zn(2+)-binding protein associated with the high-affinity ATP-binding cassette ZnuABC transporter from Escherichia coli. Although several structures of ZnuA and its homologs have been determined, details regarding metal ion stoichiometry, affinity, and specificity as well as the mechanism of metal uptake and transfer remain unclear. The crystal structures of E. coli ZnuA (Eco-ZnuA) in the apo, Zn(2+)-bound, and Co(2+)-bound forms have been determined. ZnZnuA binds at least two metal ions. The first, observed previously in other structures, is coordinated tetrahedrally by Glu59, His60, His143, and His207. Replacement of Zn(2+) with Co(2+) results in almost identical coordination geometry at this site. The second metal binding site involves His224 and several yet to be identified residues from the His-rich loop that is unique to Zn(2+) periplasmic metal binding receptors. Electron paramagnetic resonance and X-ray absorption spectroscopic data on CoZnuA provide additional insight into possible residues involved in this second site. The second site is also detected by metal analysis and circular dichroism (CD) titrations. Eco-ZnuA binds Zn(2+) (estimated K (d) < 20 nM), Co(2+), Ni(2+), Cu(2+), Cu(+), and Cd(2+), but not Mn(2+). Finally, conformational changes upon metal binding observed in the crystal structures together with fluorescence and CD data indicate that only Zn(2+) substantially stabilizes ZnuA and might facilitate recognition of ZnuB and subsequent metal transfer.

-	~~{{STRUCTURE_2ps0\| PDB=2ps0 \| SCENE= }}~~	+	Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli.,Yatsunyk LA, Easton JA, Kim LR, Sugarbaker SA, Bennett B, Breece RM, Vorontsov II, Tierney DL, Crowder MW, Rosenzweig AC J Biol Inorg Chem. 2008 Feb;13(2):271-88. Epub 2007 Nov 20. PMID:18027003<ref>PMID:18027003</ref>

-	~~===Structure and metal binding properties of ZnuA~~, a ~~periplasmic zinc transporter from Escherichia coli===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18027003}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2ps0]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018027003<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Kim, L R.]]		[[Category: Kim, L R.]]

OCA: Protected "2ps0" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 11:56:10 GMT

Protected "2ps0" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:56, 7 January 2013

OCA at 11:56, 7 January 2013

OCA — Mon, 07 Jan 2013 11:56:06 GMT

←Older revision		Revision as of 11:56, 7 January 2013
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-	{{Seed}}
	[[Image:2ps0.png\|left\|200px]]		[[Image:2ps0.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2ps0", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2ps0\| PDB=2ps0 \| SCENE= }}		{{STRUCTURE_2ps0\| PDB=2ps0 \| SCENE= }}

	===Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli===		===Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli===

-
-	<!--
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	{{ABSTRACT_PUBMED_18027003}}		{{ABSTRACT_PUBMED_18027003}}

	==About this Structure==		==About this Structure==
-	~~2PS0~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA].	+	[[2ps0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18027003~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018027003</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Kim, L R.]]		[[Category: Kim, L R.]]
Line 30:		Line 18:
	[[Category: Yatsunyk, L A.]]		[[Category: Yatsunyk, L A.]]
	[[Category: Metal transport]]		[[Category: Metal transport]]
-
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OCA at 03:56, 18 February 2009

OCA — Wed, 18 Feb 2009 03:56:06 GMT

←Older revision		Revision as of 03:56, 18 February 2009
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	==About this Structure==		==About this Structure==
-	2PS0 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA].	+	2PS0 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PS0 OCA].

	==Reference==		==Reference==
-	Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli., Yatsunyk LA, Easton JA, Kim LR, Sugarbaker SA, Bennett B, Breece RM, Vorontsov II, Tierney DL, Crowder MW, Rosenzweig AC, J Biol Inorg Chem. 2008 Feb;13(2):271-88. Epub 2007 Nov 20. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18027003 18027003]~~	+	<ref group="xtra">PMID:18027003</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Kim, L R.]]		[[Category: Kim, L R.]]
	[[Category: Rosenzweig, A C.]]		[[Category: Rosenzweig, A C.]]
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	[[Category: Metal transport]]		[[Category: Metal transport]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 12~~:40:~~24 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:56:04 2009''

OCA at 09:40, 29 July 2008

OCA — Tue, 29 Jul 2008 09:40:38 GMT

←Older revision		Revision as of 09:40, 29 July 2008
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-	[[Image:2ps0.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2ps0.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2ps0\| PDB=2ps0 \| SCENE= }}		{{STRUCTURE_2ps0\| PDB=2ps0 \| SCENE= }}

-	~~'''~~Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli~~'''~~	+	===Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli===


-	~~==Overview==~~	+	<!--
-	~~ZnuA is the periplasmic Zn(2+)~~-~~binding protein associated with the high~~-~~affinity ATP-binding cassette ZnuABC transporter from Escherichia coli. Although several structures of ZnuA and its homologs have been determined~~, ~~details regarding metal ion stoichiometry~~, ~~affinity, and specificity as well as~~ the ~~mechanism of metal uptake and transfer remain unclear. The crystal structures of E. coli ZnuA (Eco-ZnuA) in~~ the ~~apo, Zn~~(~~2+)-bound, and Co(2+)-bound forms have been determined~~. ~~ZnZnuA binds at least two metal ions~~. ~~The first, observed previously in other structures~~, is coordinated tetrahedrally by Glu59, His60, His143, and His207. Replacement of Zn(2+) with Co(2+) results in almost identical coordination geometry at this site. The second metal binding site involves His224 and several yet to be identified residues from the ~~His-rich loop that is unique to Zn(2+) periplasmic metal binding receptors~~. ~~Electron paramagnetic resonance and X~~-ray absorption spectroscopic data on CoZnuA provide additional insight into possible residues involved in this second site. The second site is also detected by metal analysis and circular dichroism (CD) titrations. Eco-ZnuA binds Zn(2+) (estimated K (d) < 20 nM), Co(2+), Ni(2+), Cu(2+), Cu(+), and Cd(2+), but not Mn(2+). Finally, conformational changes upon metal binding observed in the crystal structures together with fluorescence and CD data indicate that only Zn(2+) substantially stabilizes ZnuA and might facilitate recognition of ZnuB and subsequent metal transfer.	+	The line below this paragraph, {{ABSTRACT_PUBMED_18027003}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18027003 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18027003}}

	==About this Structure==		==About this Structure==
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	[[Category: Yatsunyk, L A.]]		[[Category: Yatsunyk, L A.]]
	[[Category: Metal transport]]		[[Category: Metal transport]]
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