2q6q - Revision history

OCA at 09:14, 21 February 2024

OCA — Wed, 21 Feb 2024 09:14:17 GMT

←Older revision		Revision as of 09:14, 21 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2q6q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q6Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[2q6q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q6Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.97Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [https://pdbe.org/2q6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [https://www.ebi.ac.uk/pdbsum/2q6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q6q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [https://pdbe.org/2q6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [https://www.ebi.ac.uk/pdbsum/2q6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q6q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/SPC42_YEAST SPC42_YEAST] Forms a polymeric layer at the periphery of the spindle pole body (SPB) central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.<ref>PMID:10339566</ref>		[https://www.uniprot.org/uniprot/SPC42_YEAST SPC42_YEAST] Forms a polymeric layer at the periphery of the spindle pole body (SPB) central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.<ref>PMID:10339566</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The spindle pole body (SPB) is a multiprotein complex that organizes microtubules in yeast. Due to its large size and association with the nuclear membrane, little is known about its detailed structure. In particular, although many SPB components and some of the interactions between them have been identified, the molecular details of how most of these interactions occur are not known. The prevalence of predicted coiled-coil regions in SPB proteins suggests that some interactions may occur via coiled coils. Here this hypothesis is supported by biochemical characterization of isolated coiled-coil peptides derived from SPB proteins. Formation of four strongly self-associating coiled-coil complexes from Spc29, Spc42, and Spc72 was demonstrated by circular dichroism (CD) spectroscopy and a fluorescence resonance energy transfer (FRET) assay. Many weaker self- and heteroassociations were also detected by CD, FRET, and/or cross-linking. The thermal stabilities of nine candidate homooligomers were assessed; six unfolded cooperatively with melting temperatures ranging from <11 to >50 degrees C. Solution studies established that coiled-coil peptides derived from Spc42 and Spc72 form parallel dimers, and this was confirmed for Spc42 by a high-resolution crystal structure. These data contribute to a growing body of knowledge that will ultimately provide a detailed model of the SPB structure.
-
-	Analysis of coiled-coil interactions between core proteins of the spindle pole body.,Zizlsperger N, Malashkevich VN, Pillay S, Keating AE Biochemistry. 2008 Nov 11;47(45):11858-68. Epub 2008 Oct 14. PMID:18850724<ref>PMID:18850724</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2q6q" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:18, 29 March 2023

OCA — Wed, 29 Mar 2023 07:18:41 GMT

←Older revision		Revision as of 07:18, 29 March 2023
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	<StructureSection load='2q6q' size='340' side='right'caption='[[2q6q]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='2q6q' size='340' side='right'caption='[[2q6q]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2q6q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q6Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2q6q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q6Q FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC42 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [https://pdbe.org/2q6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [https://www.ebi.ac.uk/pdbsum/2q6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q6q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [https://pdbe.org/2q6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [https://www.ebi.ac.uk/pdbsum/2q6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q6q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SPC42_YEAST SPC42_YEAST]] Forms a polymeric layer at the periphery of the spindle pole body (SPB) central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.<ref>PMID:10339566</ref>	+	[https://www.uniprot.org/uniprot/SPC42_YEAST SPC42_YEAST] Forms a polymeric layer at the periphery of the spindle pole body (SPB) central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.<ref>PMID:10339566</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Kim, P S~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Malashkevich, V N~~]]	+	[[Category: Kim PS]]
-	[[Category: ~~Newman, J R~~]]	+	[[Category: Malashkevich VN]]
-	[[Category: ~~Budding yeast]]~~	+	[[Category: Newman JR]]
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Spc42p]]~~	+
-	~~[[Category: Spindle pole body~~]]	+

OCA at 08:10, 25 June 2021

OCA — Fri, 25 Jun 2021 08:10:21 GMT

←Older revision		Revision as of 08:10, 25 June 2021
Line 1:		Line 1:

	==Crystal structure of Spc42p, a critical component of spindle pole body in budding yeast==		==Crystal structure of Spc42p, a critical component of spindle pole body in budding yeast==
-	<StructureSection load='2q6q' size='340' side='right' caption='[[2q6q]], [[Resolution\|resolution]] 1.97Å' scene=''>	+	<StructureSection load='2q6q' size='340' side='right'caption='[[2q6q]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2q6q]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2Q6Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2q6q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q6Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC42 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC42 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [~~http~~://pdbe.org/2q6q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2q6q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2q6q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [https://pdbe.org/2q6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [https://www.ebi.ac.uk/pdbsum/2q6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q6q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SPC42_YEAST SPC42_YEAST]] Forms a polymeric layer at the periphery of the spindle pole body (SPB) central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.<ref>PMID:10339566</ref>	+	[[https://www.uniprot.org/uniprot/SPC42_YEAST SPC42_YEAST]] Forms a polymeric layer at the periphery of the spindle pole body (SPB) central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.<ref>PMID:10339566</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Kim, P S]]		[[Category: Kim, P S]]
	[[Category: Malashkevich, V N]]		[[Category: Malashkevich, V N]]

OCA at 08:43, 29 August 2018

OCA — Wed, 29 Aug 2018 08:43:50 GMT

←Older revision		Revision as of 08:43, 29 August 2018
Line 1:		Line 1:
		+
	==Crystal structure of Spc42p, a critical component of spindle pole body in budding yeast==		==Crystal structure of Spc42p, a critical component of spindle pole body in budding yeast==
	<StructureSection load='2q6q' size='340' side='right' caption='[[2q6q]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='2q6q' size='340' side='right' caption='[[2q6q]], [[Resolution\|resolution]] 1.97Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC42 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC42 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [http://pdbe.org/2q6q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [http://www.ebi.ac.uk/pdbsum/2q6q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [http://pdbe.org/2q6q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [http://www.ebi.ac.uk/pdbsum/2q6q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2q6q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 16:55, 11 September 2015

OCA — Fri, 11 Sep 2015 16:55:22 GMT

←Older revision		Revision as of 16:55, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2q6q' size='340' side='right' caption='[[2q6q]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='2q6q' size='340' side='right' caption='[[2q6q]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2q6q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Q6Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2q6q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Q6Q FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC42 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC42 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [http://www.ebi.ac.uk/pdbsum/2q6q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [http://pdbe.org/2q6q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [http://www.ebi.ac.uk/pdbsum/2q6q PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2q6q" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Kim, P S]]		[[Category: Kim, P S]]
	[[Category: Malashkevich, V N]]		[[Category: Malashkevich, V N]]

OCA at 19:17, 25 December 2014

OCA — Thu, 25 Dec 2014 19:17:21 GMT

←Older revision		Revision as of 19:17, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [http://www.ebi.ac.uk/pdbsum/2q6q PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [http://www.ebi.ac.uk/pdbsum/2q6q PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPC42_YEAST SPC42_YEAST]] Forms a polymeric layer at the periphery of the spindle pole body (SPB) central plaque which has an essential function during SPB duplication and may facilitate attachment of the SPB to the nuclear membrane.<ref>PMID:10339566</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Kim, P S.]]	+	[[Category: Kim, P S]]
-	[[Category: Malashkevich, V N.]]	+	[[Category: Malashkevich, V N]]
-	[[Category: Newman, J R.]]	+	[[Category: Newman, J R]]
	[[Category: Budding yeast]]		[[Category: Budding yeast]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Spc42p]]		[[Category: Spc42p]]
	[[Category: Spindle pole body]]		[[Category: Spindle pole body]]

OCA at 11:19, 20 October 2014

OCA — Mon, 20 Oct 2014 11:19:37 GMT

←Older revision		Revision as of 11:19, 20 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2q6q.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Spc42p, a critical component of spindle pole body in budding yeast==
		+	<StructureSection load='2q6q' size='340' side='right' caption='[[2q6q]], [[Resolution\|resolution]] 1.97Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2q6q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Q6Q FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC42 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2q6q RCSB], [http://www.ebi.ac.uk/pdbsum/2q6q PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The spindle pole body (SPB) is a multiprotein complex that organizes microtubules in yeast. Due to its large size and association with the nuclear membrane, little is known about its detailed structure. In particular, although many SPB components and some of the interactions between them have been identified, the molecular details of how most of these interactions occur are not known. The prevalence of predicted coiled-coil regions in SPB proteins suggests that some interactions may occur via coiled coils. Here this hypothesis is supported by biochemical characterization of isolated coiled-coil peptides derived from SPB proteins. Formation of four strongly self-associating coiled-coil complexes from Spc29, Spc42, and Spc72 was demonstrated by circular dichroism (CD) spectroscopy and a fluorescence resonance energy transfer (FRET) assay. Many weaker self- and heteroassociations were also detected by CD, FRET, and/or cross-linking. The thermal stabilities of nine candidate homooligomers were assessed; six unfolded cooperatively with melting temperatures ranging from <11 to >50 degrees C. Solution studies established that coiled-coil peptides derived from Spc42 and Spc72 form parallel dimers, and this was confirmed for Spc42 by a high-resolution crystal structure. These data contribute to a growing body of knowledge that will ultimately provide a detailed model of the SPB structure.

-	~~{{STRUCTURE_2q6q\| PDB=2q6q \| SCENE= }}~~	+	Analysis of coiled-coil interactions between core proteins of the spindle pole body.,Zizlsperger N, Malashkevich VN, Pillay S, Keating AE Biochemistry. 2008 Nov 11;47(45):11858-68. Epub 2008 Oct 14. PMID:18850724<ref>PMID:18850724</ref>

-	~~===Crystal structure of Spc42p~~, a ~~critical component~~ of ~~spindle pole body in budding yeast===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18850724}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2q6q]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018850724<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Kim, P S.]]		[[Category: Kim, P S.]]

OCA: Protected "2q6q" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 18:11:28 GMT

Protected "2q6q" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:11, 7 January 2013

OCA at 18:11, 7 January 2013

OCA — Mon, 07 Jan 2013 18:11:25 GMT

←Older revision		Revision as of 18:11, 7 January 2013
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-	{{Seed}}
	[[Image:2q6q.png\|left\|200px]]		[[Image:2q6q.png\|left\|200px]]

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	{{STRUCTURE_2q6q\| PDB=2q6q \| SCENE= }}		{{STRUCTURE_2q6q\| PDB=2q6q \| SCENE= }}

	===Crystal structure of Spc42p, a critical component of spindle pole body in budding yeast===		===Crystal structure of Spc42p, a critical component of spindle pole body in budding yeast===

-
-	<!--
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	{{ABSTRACT_PUBMED_18850724}}		{{ABSTRACT_PUBMED_18850724}}

	==About this Structure==		==About this Structure==
-	~~2Q6Q~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA].	+	[[2q6q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18850724~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018850724</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Kim, P S.]]		[[Category: Kim, P S.]]
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	[[Category: Budding yeast]]		[[Category: Budding yeast]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
-	[[Category: Coiled coil]]
-	[[Category: Crystal structure]]
	[[Category: Spc42p]]		[[Category: Spc42p]]
	[[Category: Spindle pole body]]		[[Category: Spindle pole body]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 10 15:55:15 2009''

OCA at 12:55, 10 June 2009

OCA — Wed, 10 Jun 2009 12:55:15 GMT

←Older revision		Revision as of 12:55, 10 June 2009
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	==About this Structure==		==About this Structure==
-	2Q6Q is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA].	+	2Q6Q is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6Q OCA].

	==Reference==		==Reference==
-	~~Analysis of coiled-coil interactions between core proteins of the spindle pole body., Zizlsperger N, Malashkevich VN, Pillay S, Keating AE, Biochemistry. 2008 Nov 11;47(45):11858-68. Epub 2008 Oct 14.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18850724 18850724]~~	+	<ref group="xtra">PMID:18850724</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Single protein~~]]	+	[[Category: Kim, P S.]]
-	[[Category: ~~Pdbx_ordinal=~~, ~~<PDBx~~:~~audit_author~~.]]	+	[[Category: Malashkevich, V N.]]
		+	[[Category: Newman, J R.]]
	[[Category: Budding yeast]]		[[Category: Budding yeast]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
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	[[Category: Spindle pole body]]		[[Category: Spindle pole body]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jan 28 20~~:27:31 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 10 15:55:15 2009''